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    Inverse linkage oligonucleotides for chemical and enzymatic processes
    1.
    发明授权
    Inverse linkage oligonucleotides for chemical and enzymatic processes 失效
    用于化学和酶法处理的逆连接寡核苷酸

    公开(公告)号：US5462854A

    公开(公告)日：1995-10-31

    申请号：US50681

    申请日：1993-04-19

    申请人： Peter J. Coassin ,  Kenneth D. Konrad ,  Jang B. Rampal ,  Ronald M. Cook

    发明人： Peter J. Coassin ,  Kenneth D. Konrad ,  Jang B. Rampal ,  Ronald M. Cook

    IPC分类号： C12N15/09 ,  C07H21/00 ,  C07H21/04 ,  C12N15/10 ,  C12Q1/68 ,  C12P19/34

    CPC分类号： C07H21/00 ,  C12N15/1034 ,  C12Q1/6853 ,  C12Q1/6862

    摘要： Inverse Linkage Oligonucleotides ("ILO") useful in enzymatic process are disclosed. Particularly preferred ILOs are amenable to enzymatic elongation from either, or most preferably, both termini. In a particularly preferred embodiment, each terminus of an ILO has an enzymatically functional 3' group. Accordingly, under appropriate conditions and in the presence of, e.g., dNTPs, enzyme, sample DNA, and ILO comprising a first region complementary to a first region of the sample DNA and a second region complementary to a second, different region of the sample DNA, exponential amplification of the sample DNA can be effectuated.

    摘要翻译： 公开了可用于酶促过程的反向连接寡核苷酸（“ILO”）。 特别优选的ILO适于从两末端或最优选两个末端的酶促延伸。 在特别优选的实施方案中，国际劳工组织的每个末端具有酶功能3'基团。 因此，在适当的条件下和在例如dNTPs，酶，样品DNA和ILO的存在下，包含与样品DNA的第一区域互补的第一区域和与样品DNA的第二个不同区域互补的第二区域 ，可以实现样品DNA的指数扩增。

    Oligonucleotide repeat arrays
    2.
    发明授权
    Oligonucleotide repeat arrays 失效
    寡核苷酸重复阵列

    公开(公告)号：US5981185A

    公开(公告)日：1999-11-09

    申请号：US863639

    申请日：1997-05-28

    申请人： Robert S. Matson ,  Peter J. Coassin ,  Jang B. Rampal ,  Charles Thomas Caskey

    发明人： Robert S. Matson ,  Peter J. Coassin ,  Jang B. Rampal ,  Charles Thomas Caskey

    IPC分类号： C12Q1/68 ,  C07H21/00 ,  C07H21/02

    CPC分类号： C12Q1/6837 ,  C12Q1/6827 ,  C12Q1/6874

    摘要： A solid support based hybridization assay is provided which allows for the systematic and reproducible analysis of repeat and tandem repeat oligonucleotide sequences of DNA and RNA by hybridization to a reverse dot blot array comprising strings of such repeats complementary to those found in particular nucleic acid targets (e.g., analyte PCR product). An addressable library (i.e., an indexed set) of complementary repeats is synthesized on a suitable support. Preferably, the support comprises a low fluorescent background support, thereby facilitating the use of non-radioisotopic modes of detection (such as fluorescence or chemiluminescence); particularly suitable in this regard is an aminated polypropylene support or similar material. Preferred arrays permit screening of DNA and RNA samples for complete sets of particular types of nucleotide repeat sequences (e.g., all nucleotide doublet or triplet repeats).

    摘要翻译： 提供了基于固体支持的杂交测定法，其通过与包含与特定核酸靶标中发现的重复序列互补的反向斑点印迹阵列杂交，从而对DNA和RNA的重复和串联重复寡核苷酸序列进行系统和可重复的分析（ 例如分析物PCR产物）。 互补重复序列的可寻址库（即，索引集）在合适的支持上合成。 优选地，载体包含低荧光背景载体，从而有利于使用非放射性同位素检测模式（如荧光或化学发光）; 在这方面特别合适的是胺化聚丙烯支撑体或类似材料。 优选的阵列允许筛选DNA和RNA样品用于完整的特定类型的核苷酸重复序列（例如，所有核苷酸双重或三重重复）。

    Synthesis reaction column
    3.
    发明授权
    Synthesis reaction column 失效
    合成反应柱

    公开(公告)号：US5419874A

    公开(公告)日：1995-05-30

    申请号：US909552

    申请日：1992-07-06

    申请人： Peter J. Coassin ,  Edward G. Hanna ,  Carlton B. Mc Kinney ,  Jang B. Rampal ,  Glenn C. Sasaki ,  Stephen W. Wunderly

    发明人： Peter J. Coassin ,  Edward G. Hanna ,  Carlton B. Mc Kinney ,  Jang B. Rampal ,  Glenn C. Sasaki ,  Stephen W. Wunderly

    IPC分类号： C07H21/04 ,  B01J19/00 ,  B01L1/00 ,  B01L3/00 ,  C07H21/00 ,  C07K1/04 ,  C40B40/06 ,  C40B50/14 ,  C40B60/14 ,  C08F8/00

    CPC分类号： C07H21/00 ,  B01J19/0046 ,  B01L3/502 ,  C07K1/045 ,  B01J2219/00286 ,  B01J2219/00292 ,  B01J2219/00335 ,  B01J2219/00373 ,  B01J2219/00454 ,  B01J2219/005 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00686 ,  B01J2219/00722 ,  C40B40/06 ,  C40B50/14 ,  C40B60/14

    摘要： The present invention is directed to a synthesis reaction column which can be conveniently implemented in post synthesis procedures. The reaction column is configured to allow easy connection to a syringe and post synthesis reagent container without requiring connection adapters.In one embodiment, the reaction column configured in the form of a cylindrical column containing the solid-phase support and having a narrow bore tube extending at one end. The tube functions as a piercing tip for accessing a septum sealed vial containing post synthesis reagent. In another embodiment, the reaction column is configured in the form of a cylindrical barrel enclosing the solid-phase support and having a threaded mount at one end. To complement the reaction column for post synthesis procedures, a vial having a complementary threaded mount at the opening is used for containing a reagent. This vial is conveniently threaded onto the end of the barrel without adapters. Consequently, post synthesis procedures can be implemented conveniently. Both embodiments may be conveniently connected to the reagent delivery system in an automated instrument for carrying out synthesis procedures, as well as post synthesis procedures.

    摘要翻译： 本发明涉及一种合成反应塔，其可以在后合成方法中方便地实施。 反应塔被配置为允许容易地连接到注射器和后合成试剂容器而不需要连接适配器。 在一个实施方案中，反应塔以包含固相载体并且在一端延伸的窄孔管的圆柱形柱形式构成。 该管用作用于进入含有后合成试剂的隔膜密封小瓶的穿刺尖端。 在另一个实施方案中，反应塔被配置成包围固相载体并在一端具有螺纹安装座的圆柱形筒形式。 为了补充后合成方法的反应塔，在开口处具有互补螺纹安装座的小瓶被用于容纳试剂。 该小瓶方便地拧到筒的端部，而没有适配器。 因此，可以方便地实现后合成程序。 两个实施方案可以方便地连接到用于进行合成过程的自动化仪器中的试剂递送系统以及后合成程序。

    Method and apparatus for creating biopolymer arrays on a solid support surface
    5.
    发明授权
    Method and apparatus for creating biopolymer arrays on a solid support surface 失效
    在固体支撑体表面上生成生物聚合物阵列的方法和装置

    公开(公告)号：US5429807A

    公开(公告)日：1995-07-04

    申请号：US144954

    申请日：1993-10-28

    申请人： Robert S. Matson ,  Peter J. Coassin ,  Jang B. Rampal ,  Edwin M. Southern

    发明人： Robert S. Matson ,  Peter J. Coassin ,  Jang B. Rampal ,  Edwin M. Southern

    IPC分类号： C12M1/00 ,  B01J19/00 ,  C07H21/00 ,  C07H21/02 ,  C07H21/04 ,  C07K1/04 ,  C12Q1/68 ,  C40B40/06 ,  C40B60/14 ,  G01N35/10 ,  C08F2/00 ,  B01L11/00 ,  G01N33/543 ,  G05B17/00

    CPC分类号： C07H21/00 ,  B01J19/0046 ,  C07K1/045 ,  C12Q1/6874 ,  G01N35/1072 ,  B01J2219/00427 ,  B01J2219/0043 ,  B01J2219/00527 ,  B01J2219/0059 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00659 ,  B01J2219/00686 ,  B01J2219/00722 ,  C40B40/06 ,  C40B60/14

    摘要： An automated method and apparatus for performing biopolymer synthesis on a two-dimensional support surface whereby a two-dimensional matrix or array of biopolymers are obtained on the surface. An applicator in the form of a thick plate having in a surface at least one cavity in the form of an open chamber or channel is used for applying reagents to the surface of a solid phase support material. The applicator is positioned over the solid support material with the surface having the cavity sealed against the material surface. For each cavity, reagents for synthesis are introduced sequentially into one end of the cavity and collected from the other end of the cavity. A one-dimensional array of biopolymers are thus formed on the support material, where each element of the array contains a population of biopolymers having identical sequence. Further synthesis of additional biopolymers is performed by linearly moving or rotating the applicator relative to the support material and carrying out synthesis procedure so that another one-dimensional matrix or array of biopolymers are formed at an offset position to the previous array. At each overlapping region of the two arrays, a cell of biopolymers are formed which each comprises a strand from the first array and a strand from the second array. The result is a two-dimensional array of biopolymers of different sequences at each discrete cell. The arrays of discrete cell containing different biopolymers may be used to conduct hybridization reaction analysis of a biological sample.

    摘要翻译： 一种用于在二维支撑表面上进行生物聚合物合成的自动化方法和装置，由此在表面上获得二维基质或生物聚合物阵列。 厚板形式的施涂器在表面上具有至少一个开口腔或通道形式的空腔，用于将试剂施加到固相支撑材料的表面。 施用器位于固体支撑材料上方，其表面具有密封材料表面。 对于每个空腔，合成试剂依次引入空腔的一端并从空腔的另一端收集。 因此，在载体材料上形成一维生物聚合物阵列，其中阵列的每个元件含有具有相同序列的生物聚合物群。 另外的生物聚合物的进一步合成是通过相对于载体材料线性移动或旋转施加器并执行合成程序来进行的，以便在与先前阵列的偏移位置处形成另一个一维矩阵或生物聚合物阵列。 在两个阵列的每个重叠区域，形成生物聚合物的细胞，每个细胞包含来自第一阵列的链和来自第二阵列的链。 其结果是在每个离散的细胞上具有不同序列的生物聚合物的二维阵列。 含有不同生物聚合物的离散细胞阵列可用于进行生物样品的杂交反​​应分析。

    Low fluorescence assay platforms and related methods for drug discovery
    8.
    发明授权
    Low fluorescence assay platforms and related methods for drug discovery 有权
    低荧光测定平台及药物发现的相关方法

    公开(公告)号：US06730520B2

    公开(公告)日：2004-05-04

    申请号：US10120644

    申请日：2002-04-09

    申请人： Peter J. Coassin ,  Alec Tate Harootunian ,  Andrew A. Pham ,  Harry Stylli ,  Roger Y. Tsien

    发明人： Peter J. Coassin ,  Alec Tate Harootunian ,  Andrew A. Pham ,  Harry Stylli ,  Roger Y. Tsien

    IPC分类号： G01N2176

    CPC分类号： B01L3/5085 ,  B01J2219/00315 ,  B01J2219/00317 ,  B01J2219/00659 ,  B01J2219/00707 ,  B01J2219/0072 ,  B01L9/523 ,  B01L2300/021 ,  B01L2300/0829 ,  B01L2300/12 ,  C12M23/12 ,  C12Q1/00 ,  C40B60/14 ,  G01N1/30 ,  G01N21/03 ,  G01N21/6428 ,  G01N21/6452 ,  G01N33/5308 ,  G01N33/542 ,  G01N2021/0346

    摘要： One aspect of the present invention is a multi-well platform for fluorescence measurements, comprising a plurality of wells within a frame, wherein the multi-well platform has low fluorescence background. Another aspect of the present invention is a system for spectroscopic measurements, comprising reagents for an assay and a multi-well platform for fluorescence measurements. A further aspect of the present invention is a method for detecting the presence of an analyte in a sample contained in a multi-well platform by detecting light emitted from the sample. Another aspect of the present invention is a method from identifying a modulator of a biological process or target in a sample contained in a multi-well platform by detecting light emitted from the sample. Another aspect of the present invention is a composition identified by this method. A further aspect of the present invention is a method to identify a therapeutic. A further aspect of the present invention is a method of testing a therapeutic for therapeutic activity and toxicology by identifying a therapeutic using a method of the present invention and monitoring the toxicology and efficacy of the therapeutic in an in vivo model.

    摘要翻译： 本发明的一个方面是用于荧光测量的多孔平台，其包括框架内的多个孔，其中所述多孔平台具有低荧光背景。 本发明的另一方面是用于光谱测量的系统，其包括用于测定的试剂和用于荧光测量的多孔平台。 本发明的另一方面是通过检测从样品发射的光来检测多孔平台中包含的样品中分析物的存在的方法。 本发明的另一方面是通过检测从样品发射的光来识别多孔平台中包含的样品中生物过程或靶的调节剂的方法。 本发明的另一方面是通过该方法鉴定的组合物。 本发明的另一方面是鉴定治疗剂的方法。 本发明的另一方面是通过使用本发明的方法鉴定治疗剂并监测治疗剂在体内模型中的毒理学和功效来测试治疗活性和毒理学治疗剂的方法。

    Multi-well plate providing a high-density storage and assay platform
    10.
    发明授权
    Multi-well plate providing a high-density storage and assay platform 有权
    多孔板提供高密度存储和测定平台

    公开(公告)号：US07858044B2

    公开(公告)日：2010-12-28

    申请号：US10837219

    申请日：2004-04-30

    申请人： Peter J. Coassin ,  Todd Bennett ,  Brian Grot ,  David Nicol

    发明人： Peter J. Coassin ,  Todd Bennett ,  Brian Grot ,  David Nicol

    IPC分类号： B01L3/00

    CPC分类号： B01L3/50851 ,  B01L9/523 ,  B01L2200/025 ,  B01L2200/142 ,  B01L2300/0829 ,  B01L2300/12

    摘要： A dual-use, high density plate for storage and assays includes a frame including a matrix of wells. The matrix includes preferably 3456 wells with top portions being arranged preferably approximately flush with a plane of the frame. A solvent-resistant material such as cyclo-olefin polymer forms at least the bottom portions of the wells, and preferably the same solvent resistant material forms the frame, although varying from the bottoms of the wells by being rendered opaque. Evaporation control wells are preferably included at the periphery of the matrix for reducing effects of evaporation on edge wells.

    摘要翻译： 用于储存和测定的双重用途的高密度板包括包括孔的矩阵的框架。 该基质优选包括3456个孔，顶部优选地与框架的平面大致齐平。 耐溶剂材料如环烯烃聚合物至少形成孔的底部，并且优选地，相同的耐溶剂材料形成框架，尽管通过变得不透明而从孔的底部变化。 蒸发控制孔优选包含在基质的周边，以减少边缘孔蒸发的影响。