公开(公告)号：US09128953B1

公开(公告)日：2015-09-08

申请号：US12460677

申请日：2009-07-21

申请人： Pratap Singh ,  Hua Zhong

发明人： Pratap Singh ,  Hua Zhong

IPC分类号： G06F17/30

CPC分类号： G06F17/30174 ,  G06F17/30212 ,  G06F17/3023

摘要： A system for replicating a filesystem running on multiple nodes comprising an originator system and a replica system. The originator system includes a plurality of nodes. The replica system includes a plurality of nodes and a processor. The plurality of nodes of the replica system are paired with the plurality of nodes of the originator system. The processor is configured to receive an indicator of a node of the plurality of nodes of the replica system finishing replication for a view of a filesystem at a point in time. The processor is further configured to indicate that replication for the view of the filesystem is complete in the event that all replica system nodes have received their indication for the view.

摘要翻译： 用于复制在包含发起者系统和副本系统的多个节点上运行的文件系统的系统。 发起者系统包括多个节点。 复制系统包括多个节点和处理器。 复制系统的多个节点与发起者系统的多个节点配对。 所述处理器被配置为接收所述副本系统的所述多个节点中的节点的指示符，以完成针对文件系统的视图在某个时间点的复制。 处理器还被配置为指示在所有副本系统节点已经接收到其对于视图的指示的情况下，用于文件系统的视图的复制是完整的。

公开(公告)号：US20140356885A1

公开(公告)日：2014-12-04

申请号：US13905459

申请日：2013-05-30

申请人： Rafael Bartz ,  Pratap Singh ,  Liping Geng ,  Gladys Privon

发明人： Rafael Bartz ,  Pratap Singh ,  Liping Geng ,  Gladys Privon

IPC分类号： G01N33/53

CPC分类号： G01N33/54333

摘要： Methods and reagents are disclosed for preparing a support for reaction of the support with an assay molecule. In the method the support is treated with a detergent at a concentration of about 0.01% to about 5% (by weight) at a temperature of about 4° C. to about 50° C. for a period of about 1 hour to about 24 hours and subsequently the support is washed. The support is contacted with the assay molecule under conditions for covalently binding the assay molecule to the support to form a conjugate of the support and the assay molecule.

摘要翻译： 公开了用于制备载体与测定分子反应的载体的方法和试剂。 在该方法中，在约4℃​​至约50℃的温度下，以约0.01％至约5％（重量）的浓度的洗涤剂处理载体约1小时至约24 小时，随后洗涤支持。 在将分析分子共价结合到载体以形成载体和测定分子的缀合物的条件下，将支持物与测定分子接触。

公开(公告)号：US20130345357A1

公开(公告)日：2013-12-26

申请号：US13973372

申请日：2013-08-22

申请人： Bhalchandra Lele ,  Pratap Singh ,  Raphael Bartz ,  Jason A. Kellogg

发明人： Bhalchandra Lele ,  Pratap Singh ,  Raphael Bartz ,  Jason A. Kellogg

IPC分类号： C09D177/06 ,  C09D179/04 ,  C09D181/00

CPC分类号： C09D133/26 ,  C08F120/60 ,  C08F126/10 ,  C08F220/06 ,  C08F220/26 ,  C08F220/38 ,  C08F220/58 ,  C08F2800/20 ,  C09D139/06 ,  C09D177/06 ,  C09D179/04 ,  C09D181/00 ,  G01N33/533 ,  G01N33/582

摘要： A composition for use as an assay reagent comprises a solid support comprising a member of a signal producing system and a coating of a synthetic copolymer. The synthetic copolymer comprises a first polymerized monomer comprising a pendant moiety comprising a reactive functionality or a derivative of a reactive functionality and a second polymerized monomer comprising a pendant moiety comprising at least 1 carbon atoms and at least 2 heteroatoms. In some embodiments the copolymer comprises a polyethylenic backbone comprising the pendant moiety comprising a reactive functionality or a derivative of a reactive functionality and the pendant moiety comprising at least 1 carbon atom and at least 2 heteroatoms.

摘要翻译： 用作测定试剂的组合物包括固体支持物，其包括信号产生系统的成员和合成共聚物的涂层。 合成共聚物包含第一聚合单体，其包括包含反应性官能团或反应性官能团的衍生物的侧基部分和包含至少包含1个碳原子和至少2个杂原子的侧链部分的第二聚合单体。 在一些实施方案中，共聚物包含包含侧链部分的聚乙烯主链，其包含反应性官能团或反应性官能团的衍生物，并且所述侧链部分包含至少1个碳原子和至少2个杂原子。

公开(公告)号：US08394647B2

公开(公告)日：2013-03-12

申请号：US13029786

申请日：2011-02-17

申请人： Bhalchandra Lele ,  Pratap Singh ,  Asoke Chatterjee

发明人： Bhalchandra Lele ,  Pratap Singh ,  Asoke Chatterjee

IPC分类号： G01N33/544

CPC分类号： C08B37/0021 ,  C08B37/00 ,  C08L5/06 ,  C08L25/06

摘要： Methods and reagents are disclosed for reducing an amount of non-covalently bound polysaccharide on a support. The method comprises contacting a support comprising both covalently bound polysaccharide and non-covalently bound polysaccharide with an aqueous solution comprising an amount of a chaotropic agent effective to remove non-covalently bound polysaccharide from the support.

摘要翻译： 公开了减少载体上非共价结合多糖的量的方法和试剂。 该方法包括将包含共价结合的多糖和非共价结合的多糖的载体与包含一定量的有效从载体中除去非共价结合的多糖的离液剂的水溶液接触。

公开(公告)号：US20120286832A1

公开(公告)日：2012-11-15

申请号：US13300318

申请日：2011-11-18

申请人： Stéphane Le Tual ,  Pratap Singh

发明人： Stéphane Le Tual ,  Pratap Singh

IPC分类号： H03L7/00

CPC分类号： G06F13/423

摘要： The invention concerns a circuit comprising: a first circuit block (302) adapted to receive a first clock signal (CLK1) and to provide a first output data signal at a time determined by said first clock signal; a second circuit block (304) adapted to receive a second clock signal (CLK2) and to provide a second output data signal at a time determined by said second clock signal; a clock bus (314) coupled to corresponding outputs of said first and second circuit blocks for receiving a third clock signal (BCLK) based on said first and second clock signals; and a synchronization unit (312) coupled to said clock bus and adapted to sample said first and second output data signals based on said third clock signal.

摘要翻译： 本发明涉及一种电路，包括：适于接收第一时钟信号（CLK1）并在由所述第一时钟信号确定的时间提供第一输出数据信号的第一电路块（302） 适于接收第二时钟信号（CLK2）并在由所述第二时钟信号确定的时间提供第二输出数据信号的第二电路块（304） 连接到所述第一和第二电路块的相应输出的时钟总线（314），用于基于所述第一和第二时钟信号接收第三时钟信号（BCLK）; 以及耦合到所述时钟总线并适于基于所述第三时钟信号对所述第一和第二输出数据信号进行采样的同步单元（312）。

公开(公告)号：US20110306148A1

公开(公告)日：2011-12-15

申请号：US12815007

申请日：2010-06-14

申请人： Bhalchandra Lele ,  Pratap Singh ,  Raphael Bartz ,  Jason A. Kellogg

发明人： Bhalchandra Lele ,  Pratap Singh ,  Raphael Bartz ,  Jason A. Kellogg

IPC分类号： G01N33/53 ,  C08F218/02

CPC分类号： C09D133/26 ,  C08F120/60 ,  C08F126/10 ,  C08F220/06 ,  C08F220/26 ,  C08F220/38 ,  C08F220/58 ,  C08F2800/20 ,  C09D139/06 ,  C09D177/06 ,  C09D179/04 ,  C09D181/00 ,  G01N33/533 ,  G01N33/582

摘要： A composition for use as an assay reagent comprises a solid support comprising a member of a signal producing system and a coating of a synthetic copolymer. The synthetic copolymer comprises a first polymerized monomer comprising a pendant moiety comprising a reactive functionality or a derivative of a reactive functionality and a second polymerized monomer comprising a pendant moiety comprising at least 1 carbon atoms and at least 2 heteroatoms. In some embodiments the copolymer comprises a polyethylenic backbone comprising the pendant moiety comprising a reactive functionality or a derivative of a reactive functionality and the pendant moiety comprising at least 1 carbon atom and at least 2 heteroatoms.

摘要翻译： 用作测定试剂的组合物包括固体支持物，其包括信号产生系统的成员和合成共聚物的涂层。 合成共聚物包含第一聚合单体，其包括包含反应性官能团或反应性官能团的衍生物的侧基部分和包含至少包含1个碳原子和至少2个杂原子的侧链部分的第二聚合单体。 在一些实施方案中，共聚物包含包含侧链部分的聚乙烯主链，其包含反应性官能团或反应性官能团的衍生物，并且所述侧链部分包含至少1个碳原子和至少2个杂原子。

公开(公告)号：US20100233725A1

公开(公告)日：2010-09-16

申请号：US12403299

申请日：2009-03-12

申请人： Pratap Singh ,  Yi Feng Zheng ,  Liping Geng ,  Roland Janzen ,  Carsten Schelp

发明人： Pratap Singh ,  Yi Feng Zheng ,  Liping Geng ,  Roland Janzen ,  Carsten Schelp

IPC分类号： G01N33/53

CPC分类号： G01N33/78 ,  G01N33/582 ,  G01N2333/59

摘要： Methods and reagents are disclosed for conducting assays. Embodiments of the present methods and reagents are concerned with chemiluminescent reagents for determining the presence and/or amount of an analyte in a sample suspected of containing the analyte. The reagent is non-particulate and comprises a binding partner for the analyte and a chemiluminescent composition comprising an olefinic compound and a metal chelate. In embodiments of an assay, a combination is provided that comprises a sample suspected of containing the analyte, a chemiluminescent reagent as described above and a sensitizer reagent capable of generating singlet oxygen. The combination is subjected to conditions for binding of the analyte to the binding partner for the analyte. The sensitizer is activated and the amount of luminescence generated by the chemiluminescent composition is detected wherein the amount of luminescence is related to the amount of the analyte in the sample.

摘要翻译： 公开了用于进行测定的方法和试剂。 本发明方法和试剂的实施方案涉及用于确定怀疑含有分析物的样品中分析物的存在和/或量的化学发光试剂。 试剂是非颗粒的并且包含用于分析物的结合配偶体和包含烯属化合物和金属螯合物的化学发光组合物。 在测定的实施方案中，提供了包含疑似含有分析物的样品，如上所述的化学发光试剂和能够产生单线态氧的敏化剂的组合。 该组合经受用于将分析物与分析物的结合配偶体结合的条件。 敏化剂被激活，并且检测由化学发光组合物产生的发光量，其中发光量与样品中分析物的量相关。

公开(公告)号：US5948630A

公开(公告)日：1999-09-07

申请号：US689004

申请日：1996-08-05

申请人： Pratap Singh ,  Randy W. Johnson

发明人： Pratap Singh ,  Randy W. Johnson

IPC分类号： G01N33/531 ,  C12Q1/42 ,  G01N33/543 ,  G01N33/58 ,  A01N43/78 ,  C12N9/16 ,  G01N33/53

CPC分类号： C12Q1/42 ,  G01N33/54393 ,  G01N33/581

摘要： A composition and method are disclosed to reduce the effects that endogenous alkaline phosphatase found in some human bodily fluid samples have on solid phase immunoassay systems. The composition comprises a detergent, inhibitors to alkaline phosphatase, and a buffer and may contain a substrate to alkaline phosphatase.

摘要翻译： 公开了一种组合物和方法，以减少在一些人体液样品中发现的内源性碱性磷酸酶对固相免疫测定系统的影响。 组合物包含洗涤剂，碱性磷酸酶抑制剂和缓冲液，并且可以含有碱性磷酸酶的底物。

公开(公告)号：US5898005A

公开(公告)日：1999-04-27

申请号：US227364

申请日：1994-04-13

申请人： Pratap Singh ,  Fred Moll, III ,  Peter Cronin ,  Spencer H. Lin ,  Charles Ferzli ,  Kent Koski ,  Richard Saul

发明人： Pratap Singh ,  Fred Moll, III ,  Peter Cronin ,  Spencer H. Lin ,  Charles Ferzli ,  Kent Koski ,  Richard Saul

IPC分类号： G01N33/547 ,  G01N33/543 ,  G01N33/545 ,  G01N33/552

CPC分类号： G01N33/54306 ,  G01N33/545 ,  G01N33/552 ,  Y10S436/823

摘要： Methods are provided for conducting specific binding assays to determine the concentration or presence of at least one analyte in a sample. Dendrimer-reagent preparations with particular analyte specificities are mixed in solution with a sample to form dendrimer-reagent-sample complexes. The complexes are then immobilized on a solid phase. Immobilization is facilitated by coupling specific binding assay reagents such as polypeptide receptors or analytes with water soluble polymers. Such water soluble polymers, for example star polymers such as dendrimers, provide production advantages of lot-to-lot uniformity and homogeneity, and can enhance sensitivity due to low non-specific binding to the solid phase.

摘要翻译： 提供了用于进行特异性结合测定以确定样品中至少一种分析物的浓度或存在的方法。 具有特定分析物特异性的树枝状反应剂制剂在溶液中与样品混合以形成树枝状聚合物 - 试剂 - 样品复合物。 然后将络合物固定在固相上。 通过将特异性结合测定试剂如多肽受体或分析物与水溶性聚合物偶联来促进固定化。 这种水溶性聚合物，例如星形聚合物，例如树枝状聚合物，提供了批次均匀性和均匀性的生产优点，并且由于低固定相的非特异性结合而提高了灵敏度。

公开(公告)号：US5764071A

公开(公告)日：1998-06-09

申请号：US583321

申请日：1996-01-05

申请人： Benson Chan ,  Hong H. Chan ,  Pratap Singh

发明人： Benson Chan ,  Hong H. Chan ,  Pratap Singh

IPC分类号： G01R1/04 ,  H05K3/32 ,  G01R31/02

CPC分类号： G01R1/0408 ,  H05K3/325

摘要： A method in system for the testing of the electrical characteristics of an electronic module mounted on a printed circuit board. The method and system monitors selected signals from the Bottom Signal Pads of the electronic module. Thus, eliminating the need for a prototype or specialized printed circuit board in order to perform desired testing of signals.

摘要翻译： 一种用于测试安装在印刷电路板上的电子模块的电气特性的系统中的方法。 该方法和系统监视来自电子模块的底部信号垫的选定信号。 因此，为了执行期望的信号测试，不需要原型或专用印刷电路板。