-
21.发明授权公开(公告)号：US09139607B2
公开(公告)日：2015-09-22
申请号：US10540723
申请日：2003-12-25
IPC分类号： C07H1/08 , C07C239/20
CPC分类号： C07H1/08 , C07C239/20 , C07H1/06 , C07H3/06 , C07K1/14 , C07K14/77 , C07K14/79 , C07K16/00 , G01N33/5308 , G01N33/6842 , G01N2333/77 , G01N2333/79 , G01N2400/00 , G01N2560/00
摘要： The present invention provides substance which can specifically interact with sugar chains. Further, the present invention provides a method for separating, concentrating, or purifying sugar chains or a sugar chain-containing substance in a sample, comprising the steps of: a) contacting a sugar chain-trapping carrier comprising a substance which can specifically interact with sugar chains with the sample in a fluid phase under conditions that the sugar chain-trapping carrier can react with the sugar chains or sugar chain-containing substance; b) isolating a composite of the sugar chain-trapping carrier and the sugar chains or sugar chain-containing substance from the fluid phase; and c) exposing the composite to the conditions that the interaction between the sugar chain-trapping carrier and the sugar chains or sugar chain-containing substance is at least partially eliminated.
摘要翻译： 本发明提供可以与糖链特异性相互作用的物质。 此外，本发明提供了一种用于在样品中分离，浓缩或纯化糖链或含糖链物质的方法，包括以下步骤：a）使含糖链捕获载体与包含可特异性相互作用的物质 在链条捕获载体可以与糖链或含糖链物质反应的条件下，样品在液相中的糖链; b）从流体相中分离捕获糖链的载体和糖链或含糖链的物质的复合物; 以及c）将所述复合材料暴露于所述糖链捕获载体和所述糖链或含糖链物质之间的相互作用被至少部分消除的条件。
-
公开(公告)号：US20090306291A1
公开(公告)日：2009-12-10
申请号：US12309945
申请日：2007-08-06
申请人： Hideyuki Shimaoka , Shinichiro Nishimura , Yasuro Shinohara , Yoshiaki Miura , Jun-ichi Furukawa
发明人： Hideyuki Shimaoka , Shinichiro Nishimura , Yasuro Shinohara , Yoshiaki Miura , Jun-ichi Furukawa
IPC分类号： C08G81/00
CPC分类号： C08J3/24 , C07C233/20 , C07C271/08 , C07C281/02 , C08F8/30 , C08F122/38 , C08F2222/1013 , G01N33/5308 , G01N2030/027 , G01N2030/8813 , G01N2030/8836 , Y02P20/55 , Y10T428/2982 , C08F220/60
摘要： The present invention provides a method for preparing a sample characterized by binding a substance A containing a hydrazide group to a sugar chain and/or a sugar derivative via hydrazone formation between the hydrazide group of the substance A and the reducing end of the sugar chain and/or the sugar derivative thereby to enable the separation and purification of the sugar chain and/or the sugar derivative for an analytical sample from a biological sample containing the sugar chain and/or the sugar derivative by a simple operation.
摘要翻译： 本发明提供一种制备样品的方法，其特征在于，通过在物质A的酰肼基和糖链的还原端之间形成腙将含有酰肼基团的物质A与糖链和/或糖衍生物结合， /或糖衍生物，从而能够通过简单的操作从含有糖链和/或糖衍生物的生物样品中分离和纯化分析样品的糖链和/或糖衍生物。
-
23.发明申请Process for synthesis of mucin-type peptides and muc1-related glycopeptides 审中-公开用于合成粘蛋白型肽和muc1相关糖肽的方法公开(公告)号：US20090263858A1
公开(公告)日：2009-10-22
申请号：US11663081
申请日：2005-09-14
CPC分类号： C12P21/005 , C07K14/4727 , C12P21/06
摘要： The invention aims at providing novel compounds useful as primer in producing mucin-type glycopeptides which are useful in a wide field including materials for biochemical research, drugs, and food and the production of which was difficult in the prior art; and a process for the production of glycopeptides by using the primers. The aim is attained by providing novel glycopeptide derivatives (represented by the general formula (I)) which each bear an aldehyde or ketone group at the end and each contain an amino acid residue cleavable with a protease; and an easy and simple process for the production of glycopeptides by using the derivative as the primer.
摘要翻译： 本发明旨在提供可用作生产粘蛋白型糖肽的引物的新化合物，其可用于广泛的领域，包括用于生物化学研究，药物和食品的材料，并且其生产在现有技术中是困难的; 以及通过使用引物生产糖肽的方法。 目的是通过提供新的糖肽衍生物（由通式（I）表示），其末端具有醛基或酮基，并且各自含有可用蛋白酶切割的氨基酸残基; 以及通过使用该衍生物作为引物制备糖肽的简单和简单的方法。
-
公开(公告)号：US20090206245A1
公开(公告)日：2009-08-20
申请号：US10594848
申请日：2005-03-29
CPC分类号： C07C323/12 , C07D339/04 , C07H15/04 , C07H15/08
摘要： It is an object of the present invention to provide a novel mass spectrometry method which overcomes the conventional problems mentioned above, which can analyze at a high sensitivity and high accuracy a chemical reaction on a surface of a self-organized membrane bound to a metal, and which can be applied to analysis of structures of a sugar chain in future. According to the present invention, a method for performing mass spectrometry of sulfur atom-containing derivatives of an organic residue, characterized in that the method includes ionizing a metal-organic residue complex into the derivatives, wherein the complex has the organic residue bound through a sulfur atom to the metal is provided, thereby solving the above problems.
摘要翻译： 本发明的目的是提供一种克服上述常规问题的新型质谱方法，其可以高灵敏度和高精度地分析与金属结合的自组织膜的表面上的化学反应， 并且可以应用于将来对糖链结构的分析。 根据本发明，一种对有机残基的含硫原子的衍生物进行质谱分析的方法，其特征在于，所述方法包括将金属 - 有机残基配合物离子化为所述衍生物，其中所述络合物的有机残基通过 提供硫原子与金属，从而解决上述问题。
-
公开(公告)号：US07556962B2
公开(公告)日：2009-07-07
申请号：US11061536
申请日：2005-02-18
CPC分类号： C12P19/00 , B01J20/3242 , B01J2220/603 , C12N11/00 , C12P21/005 , C12P21/06
摘要： The present invention provides a sugar chain purification instrument which can continuously and efficiently purify the sugar chain. According to the present invention, the instrument includes an immobilized protease column in which a carrier which can be packed in the column and on which protease is immobilized is packed and an immobilized glycopeptidase column in which a carrier which can be packed in the column and on which glycopeptidase is immobilized is packed, these columns being connected in series, and the reaction is carried out while continuously passing a glycoprotein to be decomposed together with a suitable buffer solution through the above columns. The step of liberation of sugar chain from the glycoprotein is highly simplified and labor-saving, and liberation of the sugar chain can be moderately and simply performed.
摘要翻译： 本发明提供一种可连续高效地纯化糖链的糖链纯化装置。 根据本发明，仪器包括固定化的蛋白酶柱，其中可以填充在柱中并且固定有蛋白酶的载体和固定的糖肽酶柱，其中可以包装在柱中的载体和 将这些糖肽酶固定化，将这些柱串联连接，在连续通过糖蛋白的同时进行反应，并通过上述柱与合适的缓冲溶液一起分解。 从糖蛋白释放糖链的步骤被高度简化和省力，可以适度简单地进行糖链的释放。
-
公开(公告)号：US20070207457A1
公开(公告)日：2007-09-06
申请号：US10551156
申请日：2004-03-24
CPC分类号： G01N33/543 , C12Q1/6837 , G01N33/5308 , C12Q2525/205
摘要： A microarray originally developed for analyzing intracellular RNA expressions can be used to easily load polynucleotides onto a chip and to instantaneously determine polynucleotides (aptamers) capable of binding to a target molecule. Namely, the present inventors discovered that a microarray can be used to rapidly and efficiently obtain aptamers that bind to a target molecule.
摘要翻译： 最初用于分析细胞内RNA表达的微阵列可用于容易地将多核苷酸加载到芯片上并且瞬时确定能够结合靶分子的多核苷酸（适体）。 也就是说，本发明人发现微阵列可用于快速有效地获得与目标分子结合的适体。
-
27.发明申请公开(公告)号：US20060188996A1
公开(公告)日：2006-08-24
申请号：US10540723
申请日：2003-12-25
CPC分类号： C07H1/08 , C07C239/20 , C07H1/06 , C07H3/06 , C07K1/14 , C07K14/77 , C07K14/79 , C07K16/00 , G01N33/5308 , G01N33/6842 , G01N2333/77 , G01N2333/79 , G01N2400/00 , G01N2560/00
摘要： The present invention provides substance which can specifically interact with sugar chains. Further, the present invention provides a method for separating, concentrating, or purifying sugar chains or a sugar chain-containing substance in a sample, comprising the steps of: a) contacting a sugar chain-trapping carrier comprising a substance which can specifically interact with sugar chains with the sample in a fluid phase under conditions that the sugar chain-trapping carrier can react with the sugar chains or sugar chain-containing substance; b) isolating a composite of the sugar chain-trapping carrier and the sugar chains or sugar chain-containing substance from the fluid phase; and c) exposing the composite to the conditions that the interaction between the sugar chain-trapping carrier and the sugar chains or sugar chain-containing substance is at least partially eliminated.
摘要翻译： 本发明提供可以与糖链特异性相互作用的物质。 此外，本发明提供了一种用于在样品中分离，浓缩或纯化糖链或含糖链物质的方法，包括以下步骤：a）使含糖链捕获载体与包含可特异性相互作用的物质 在链条捕获载体可以与糖链或含糖链物质反应的条件下，样品在液相中的糖链; b）从流体相中分离捕获糖链的载体和糖链或含糖链的物质的复合物; 以及c）将所述复合材料暴露于所述糖链捕获载体和所述糖链或含糖链物质之间的相互作用被至少部分消除的条件。
-
28.发明申请Novel azasugar derivative and drug containing the same as the active ingredient 审中-公开新的azasugar衍生物和含有与活性成分相同的药物公开(公告)号：US20050137221A1
公开(公告)日：2005-06-23
申请号：US10919475
申请日：2004-08-17
IPC分类号： A61K31/445 , A61P17/00 , A61P43/00 , C07D211/96 , C07D491/04 , C07D491/056 , C07D491/02 , A61K31/4741
CPC分类号： C07D491/04 , A61K31/445 , C07D211/96
摘要： A novel azasugar derivative, and a drug containing the same as an active ingredient are disclosed. The drug is useful for treatment of keratinocyte-proliferative diseases.
摘要翻译： 公开了一种新型的azasagar衍生物和含有其作为活性成分的药物。 该药物可用于治疗角化细胞增生性疾病。
-
公开(公告)号：US08877454B2
公开(公告)日：2014-11-04
申请号：US12681573
申请日：2008-10-03
申请人： Shinichiro Nishimura , Yasuro Shinohara , Yoshiaki Miura , Hiroshi Yamazaki , Michio Horiuchi , Hiroaki Motoki , Toshiharu Kuroda , Yoko Kita , Mika Nakano
发明人： Shinichiro Nishimura , Yasuro Shinohara , Yoshiaki Miura , Hiroshi Yamazaki , Michio Horiuchi , Hiroaki Motoki , Toshiharu Kuroda , Yoko Kita , Mika Nakano
CPC分类号： G01N33/6848 , G01N30/7233 , G01N2400/00 , H01J49/00
摘要： To provide an autoanalyzer for analyzing a sugar chain contained in a biological sample, in particular, serum. Namely, it is intended to provide a method of analyzing a sugar chain in a sample, which comprises the following steps: A) the sugar chain-releasing step of releasing the sugar chain in the sample; B) the detection sample-preparing step of preparing the released sugar chain for detection; and, in the case of conducting mass spectrometry using a plate, C) the step of forming a plate for the mass spectrometry having the captured sugar chain dotted thereon which comprises the step of providing the tagged sugar chain sample solution obtained in the step B) on a collection plate; and, if required, the step of conducting an operation in a solid phase support-enclosed plate to form the plate for mass spectrometry; and D) the step of analyzing the sugar chain to be assayed.
摘要翻译： 提供一种用于分析生物样品，特别是血清中所含的糖链的自动分析仪。 即，提供一种分析样品中的糖链的方法，其包括以下步骤：A）释放样品中的糖链的糖链释放步骤; B）制备释放的糖链进行检测的检测样品制备步骤; 并且在使用板进行质谱分析的情况下，C）形成具有点状捕获的糖链的用于质谱的板的步骤，其包括提供步骤B）中获得的标记的糖链样品溶液的步骤， 在收集板上; 如果需要，在固相支持封闭板中进行操作以形成质谱板的步骤; 和D）分析要测定的糖链的步骤。
-
公开(公告)号：US08232079B2
公开(公告)日：2012-07-31
申请号：US11197335
申请日：2005-08-05
CPC分类号： C12P19/30
摘要： After various sugar nucleotide solutions and glycosyltransferases (or primers) have been mixed, they are introduced into a reaction tank (column) with primers (or glycosyltransferases) immobilized thereon. Then solutions coming out of the reaction tank are led to an ultrafiltration column. The oligosaccharide synthesizer according to the present invention is equipped with a flow path for ensuring that glycosyltransferases or primers separated by the ultrafiltration column are returned into a container for storing each solution in a sample injector.
摘要翻译： 在各种糖核苷酸溶液和糖基转移酶（或引物）已被混合之后，将它们引入固定在其上的引物（或糖基转移酶）的反应罐（柱）中。 然后将从反应槽出来的溶液引入超滤柱。 根据本发明的寡糖合成仪配备有用于确保由超滤塔分离的糖基转移酶或引物返回到用于将每种溶液存储在样品注射器中的容器中的流动路径。
-
-
-
-
-
-
-
-
-
搜索结果
41 条记录 (耗时 0.045 秒)
