公开(公告)号：US20130323382A1

公开(公告)日：2013-12-05

申请号：US13941342

申请日：2013-07-12

申请人： Solazyme, Inc.

发明人： Scott Franklin ,  Aravind Somanchi ,  George Rudenko ,  Riyaz Bhat ,  Xinhua Zhao ,  Risha Bond ,  Walter Rakitsky ,  Alejandro Marangoni ,  Diza Braksmayer

IPC分类号： C12P7/64 ,  C07J9/00 ,  A23D9/00 ,  C07C57/03

CPC分类号： C12P7/6409 ,  A23D9/00 ,  A23D9/013 ,  C07C57/03 ,  C07J9/00 ,  C11B1/00 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/2402 ,  C12N15/113 ,  C12N15/8247 ,  C12N2310/531 ,  C12P7/64 ,  C12P7/6445 ,  C12P7/6463 ,  C12P7/6472 ,  C12P33/00 ,  C12R1/89 ,  C12Y203/01015 ,  C12Y203/0102 ,  C12Y203/01041 ,  C12Y203/01051 ,  C12Y203/01179 ,  C12Y203/0118 ,  C12Y302/01014 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P20/52

摘要： Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsatturated-saturated type.

摘要翻译： 重组DNA技术用于产生产生具有所需脂肪酸谱和区域特异性或立体特异性谱的甘油三酯油的含油重组细胞。 基因操作包括编码硬脂酰-ACP脱氧酶，δ12脂肪酸去饱和酶，酰基-ACP硫酯酶，酮酰基-ACP合成酶和溶血磷脂酰基转移酶的那些。 所生产的油可以具有增强的氧化或热稳定性，或可用作煎炸油，起酥油，卷入起酥油，回火脂肪，可可脂替代物，润滑剂或用作各种化学方法的原料。 脂肪酸分布可以富集于中链轮廓，或者油可以富集饱和不饱和饱和型甘油三酸酯。

公开(公告)号：US20130316410A1

公开(公告)日：2013-11-28

申请号：US13865974

申请日：2013-04-18

申请人： Solazyme, Inc.

发明人： Scott Franklin ,  Aravind Somanchi ,  George Rudenko ,  Riyaz Bhat ,  Xinhua Zhao ,  Risha Bond ,  Walter Rakitsky ,  Alejandro Marangoni ,  Diza Braksmayer

IPC分类号： C12P7/64

CPC分类号： C12P7/6409 ,  A23D9/00 ,  A23D9/013 ,  C07C57/03 ,  C07J9/00 ,  C11B1/00 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/2402 ,  C12N15/113 ,  C12N15/8247 ,  C12N2310/531 ,  C12P7/64 ,  C12P7/6445 ,  C12P7/6463 ,  C12P7/6472 ,  C12P33/00 ,  C12R1/89 ,  C12Y203/01015 ,  C12Y203/0102 ,  C12Y203/01041 ,  C12Y203/01051 ,  C12Y203/01179 ,  C12Y203/0118 ,  C12Y302/01014 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P20/52

摘要： Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.

摘要翻译： 重组DNA技术用于产生产生具有所需脂肪酸谱和区域特异性或立体特异性谱的甘油三酯油的含油重组细胞。 基因操作包括编码硬脂酰-ACP脱氧酶，δ12脂肪酸去饱和酶，酰基-ACP硫酯酶，酮酰基-ACP合成酶和溶血磷脂酰基转移酶的那些。 所生产的油可以具有增强的氧化或热稳定性，或可用作煎炸油，起酥油，卷入起酥油，回火脂肪，可可脂替代物，润滑剂或用作各种化学方法的原料。 脂肪酸分布可以富集中链型，或者油可富含饱和不饱和饱和型甘油三酸酯。

公开(公告)号：US20160186191A1

公开(公告)日：2016-06-30

申请号：US14975016

申请日：2015-12-18

申请人： Solazyme, Inc.

发明人： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walter Rakitsky

IPC分类号： C12N15/79 ,  C12N9/02 ,  C12N9/26 ,  C12N9/40 ,  C12P7/64 ,  C12N9/16

CPC分类号： C12N15/79 ,  A23D7/00 ,  A23D9/00 ,  A23K20/158 ,  C11B1/00 ,  C11C3/00 ,  C12N9/0071 ,  C12N9/16 ,  C12N9/2431 ,  C12N9/2465 ,  C12N15/67 ,  C12N15/8216 ,  C12P7/64 ,  C12P7/6445 ,  C12P7/6463 ,  C12Y114/19001 ,  C12Y301/02 ,  C12Y301/02014 ,  C12Y302/01022 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P30/20 ,  Y02T50/678

摘要： Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.

摘要翻译： 提供了用于在重组微生物中生产油，燃料，油化合物和其它化合物的方法和组合物，包括含油微生物和这种微生物的低成本培养的方法。 包含编码例如脂肪酶，蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖降解酶，酮酰基-ACP合成酶，脂肪酰基-ACP硫酯酶，脂肪酰辅酶A的外源基因的微藻细胞 /醛还原酶，脂肪酰辅酶A还原酶，脂肪醛还原酶，脂肪醛脱羰酶和/或酰基载体蛋白质可用于制造运输燃料如可再生柴油，生物柴油和可再生喷气燃料，以及油脂化学品 例如功能流体，表面活性剂，肥皂和润滑剂。

公开(公告)号：US20150344917A1

公开(公告)日：2015-12-03

申请号：US14730671

申请日：2015-06-04

申请人： Solazyme, Inc.

发明人： Scott Franklin ,  Walter Rakitsky ,  George Rudenko ,  Xinhua Zhao ,  Felipe Arana Rodriguez ,  Wenhua Lu ,  Janice Wee

IPC分类号： C12P7/64 ,  A23D9/02 ,  C11B3/00 ,  H01B3/20 ,  C10M169/04

CPC分类号： C12P7/6463 ,  A23D9/007 ,  A23D9/02 ,  C10M169/041 ,  C11B1/025 ,  C11B1/06 ,  C11B1/10 ,  C11B3/001 ,  C11B3/006 ,  C11B3/008 ,  C11B3/06 ,  C11B3/10 ,  C11B3/14 ,  C12N9/2442 ,  C12N15/79 ,  C12P7/6409 ,  C12Y301/02014 ,  H01B3/20 ,  H01F27/105 ,  H01F27/321

摘要： Methods and compositions for the production of dielectric fluids from lipids produced by microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a lipid pathway modification enzyme, a fatty acyl-ACP thioesterase, a desaturase, a fatty acyl-CoA/aldehyde reductase, and/or an acyl carrier protein are useful in manufacturing dielectric fluids.

摘要翻译： 提供了由微生物产生的脂质生产介电流体的方法和组合物，包括含油微生物和这种微生物的低成本培养方法。 含有编码例如蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖分解酶，脂质途径修饰酶，脂肪酰基-ACP硫酯酶，去饱和酶，脂肪酰辅酶A /醛的外源基因的微藻细胞 还原酶和/或酰基载体蛋白质可用于制造介电流体。

公开(公告)号：US20150125914A1

公开(公告)日：2015-05-07

申请号：US14506491

申请日：2014-10-03

申请人： SOLAZYME, INC.

发明人： Scott Franklin ,  Aravind Somanchi ,  George Rudenko ,  Riyaz Bhat ,  Xinhua Zhao ,  Jeffrey L. Moseley

IPC分类号： C12P7/64 ,  C11C1/00 ,  C11D9/00

CPC分类号： C12P7/64 ,  C11C1/002 ,  C11D9/00 ,  C12P7/6409 ,  C12Y203/01041 ,  C12Y301/02014

摘要： Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desaturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.

摘要翻译： 重组DNA技术用于产生产生具有所需脂肪酸谱和区域特异性或立体特异性谱的甘油三酯油的含油重组细胞。 基因操作包括编码硬脂酰-ACP去饱和酶，δ12脂肪酸去饱和酶，酰基-ACP硫酯酶，酮酰基-ACP合成酶和溶血磷脂酰基转移酶的那些。 所生产的油可以具有增强的氧化或热稳定性，或可用作煎炸油，起酥油，卷入起酥油，回火脂肪，可可脂替代物，润滑剂或用作各种化学方法的原料。 脂肪酸分布可以富集中链型，或者油可富含饱和不饱和饱和型甘油三酸酯。

公开(公告)号：US20140256024A1

公开(公告)日：2014-09-11

申请号：US14285354

申请日：2014-05-22

申请人： Solazyme, Inc.

发明人： Scott Franklin ,  Aravind Somanchi ,  Karen Espina ,  George Rudenko ,  Penelope Chua

IPC分类号： C12N9/24

CPC分类号： C12P7/6427 ,  C07C1/2078 ,  C07K2319/01 ,  C10L1/02 ,  C12N9/00 ,  C12N9/16 ,  C12N9/2402 ,  C12N15/79 ,  C12P7/20 ,  C12P7/64 ,  C12P7/6409 ,  C12P7/6418 ,  C12P7/6463 ,  C12P7/649 ,  C12Y301/02014 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P20/52 ,  Y02P60/877 ,  Y02T50/678

摘要： Novel gene sequences from microalgae are disclosed, as well as novel gene sequences useful in the manufacture of triglyceride oils. Also disclosed are sequences and vectors that allow microalgae to be cultivated on sugar cane and sugar beets as a feedstock. In some embodiments, the vectors are useful for the purpose of performing targeted modifications to the nuclear genome of heterotrophic microalgae.

摘要翻译： 公开了来自微藻的新型基因序列，以及可用于制备甘油三酯油的新型基因序列。 还公开了允许将微藻种植在甘蔗和甜菜作为原料的序列和载体。 在一些实施方案中，载体可用于进行对异养微藻的核基因组的靶向修饰。

公开(公告)号：US20130323823A1

公开(公告)日：2013-12-05

申请号：US13941353

申请日：2013-07-12

申请人： Solazyme, Inc.

发明人： Scott Franklin ,  Aravind Somanchi ,  George Rudenko ,  Riyaz Bhat ,  Xinhua Zhao ,  Risha Bond ,  Walter Rakitsky ,  Alejandro Marangoni ,  Diza Braksmayer

IPC分类号： C12P7/64

CPC分类号： C12P7/6409 ,  A23D9/00 ,  A23D9/013 ,  C07C57/03 ,  C07J9/00 ,  C11B1/00 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/2402 ,  C12N15/113 ,  C12N15/8247 ,  C12N2310/531 ,  C12P7/64 ,  C12P7/6445 ,  C12P7/6463 ,  C12P7/6472 ,  C12P33/00 ,  C12R1/89 ,  C12Y203/01015 ,  C12Y203/0102 ,  C12Y203/01041 ,  C12Y203/01051 ,  C12Y203/01179 ,  C12Y203/0118 ,  C12Y302/01014 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P20/52

摘要： Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.