公开(公告)号：US4730076A

公开(公告)日：1988-03-08

申请号：US942146

申请日：1986-12-16

申请人： Tadashi Fujii ,  Hiroshi Yagiuchi ,  Akikazu Mitsunobu ,  Shigeru Aoki ,  Makoto Tsuda

发明人： Tadashi Fujii ,  Hiroshi Yagiuchi ,  Akikazu Mitsunobu ,  Shigeru Aoki ,  Makoto Tsuda

IPC分类号： C07K5/075 ,  C07C101/02

CPC分类号： C07K5/0613

摘要： The present invention relates to a process for producing .alpha.-aspartyl-phenylalanine ester represented by the general formula ##STR1## wherein R' represents an alkyl group having 1 to 4 carbon atoms, by reducing an N-protected-N-hydroxymethyl-.alpha.-aspartyl-phenylalanine ester represented by the general formula ##STR2## wherein R represents an organic mioety which can be substituted reductively by hydrogen, and R' is as defined above, with hydrogen gas or formic acid, in the presence of a reduction catalyst and an aromatic primary amine. According to the present invention, there can be obtained a high purity .alpha.-aspartyl-phenylalanine ester with a high yield.

摘要翻译： 本发明涉及通过还原N-保护的N-羟甲基-α-谷氨酰胺来制备由通式“IMAGE”表示的α-天门冬酰胺 - 苯丙氨酸酯的方法，其中R'代表具有1至4个碳原子的烷基， 由通式表示的天冬氨酰基 - 苯丙氨酸酯，其中R表示可在氢还原催化剂和还原催化剂存在下用氢气或甲酸还原的氢被还原反应的R'和R'定义的有机二氧化物， 芳族伯胺。 根据本发明，可以高产率地得到高纯度的α-天冬氨酰基苯丙氨酸酯。

公开(公告)号：US4597292A

公开(公告)日：1986-07-01

申请号：US725797

申请日：1985-04-22

申请人： Tadashi Fujii ,  Yoshinori Hayakawa

发明人： Tadashi Fujii ,  Yoshinori Hayakawa

IPC分类号： G01N29/44 ,  G01N29/34 ,  G01S7/52 ,  G01S15/89 ,  G01N29/04

CPC分类号： G01S15/8952 ,  G01N29/0645 ,  G01N29/069 ,  G01N29/245 ,  G01N29/348 ,  G01S15/8997 ,  G01S7/52036

摘要： An ultrasonic transducer is adapted to transmit spherical ultrasonic waves which have a plurality of frequencies into an object under examination while being scanned in a direction at right angles to the direction of transmission. By repeating this operation, echo signals are obtained, on the basis of which a B-scan distribution image of the echo signals is obtained through use of a synthetic aperture method. Using the distribution image, there is determined position information relating to positions of interest in the object as indicated in the distribution image, as well as scanning positions of the ultrasonic transducer situated on extensions of straight lines connecting the positions of interest. The attenuation coefficient of the object is measured based on echo signals at the scanning positions.

摘要翻译： 超声波换能器适于将具有多个频率的球面超声波在与传播方向成直角的方向扫描的同时将被检测物体发送到被检查物体中。 通过重复该操作，获得回波信号，基于此，通过使用合成孔径法获得回波信号的B扫描分布图像。 使用分布图像，确定与分布图像中指示的对象中感兴趣的位置相关的位置信息，以及位于连接感兴趣的位置的直线的延伸部上的超声波换能器的扫描位置。 基于扫描位置处的回波信号测量物体的衰减系数。

公开(公告)号：US4594895A

公开(公告)日：1986-06-17

申请号：US651963

申请日：1984-09-18

申请人： Tadashi Fujii

发明人： Tadashi Fujii

IPC分类号： A61B8/14 ,  A61B8/00 ,  G01N29/00 ,  G01N29/04 ,  G01N29/11 ,  G01N29/27 ,  G01N29/44 ,  G01S15/88

CPC分类号： G01N29/27 ,  G01N29/11 ,  G01N2291/048

摘要： A method and apparatus wherein two probes are arranged to oppose each other on either side of an object under investigation and each of two probes emits ultrasonic waves to measure the acoustic characteristics of the object respectively.Mean attenuation coefficients for small intervals can be calculated solely from the received echo signals by processing signals indicative of echos of the ultrasonic waves from the two probes. Reflection coefficients can be calculated from the mean attenuation coefficients obtained. In addition, a distribution image of either of the mean attenuation coefficient and reflection coefficient distributions are obtained from these mean attenuation coefficients and reflection coefficients for the small intervals.

摘要翻译： 一种方法和装置，其中两个探针被布置为在被研究物体的任一侧彼此相对，并且两个探针中的每一个分别发射超声波以分别测量物体的声学特性。 通过处理表示来自两个探针的超声波的回波的信号，可以仅从接收的回波信号计算小间隔的平均衰减系数。 可以从获得的平均衰减系数计算反射系数。 此外，从这些平均衰减系数和小间隔的反射系数中获得平均衰减系数和反射系数分布中的任一个的分布图像。

公开(公告)号：US4592000A

公开(公告)日：1986-05-27

申请号：US504235

申请日：1983-06-14

申请人： Hideo Ishizaka ,  Tadashi Fujii

发明人： Hideo Ishizaka ,  Tadashi Fujii

IPC分类号： G01K7/24 ,  G01K7/42

CPC分类号： G01K7/245 ,  G01K7/42

摘要： An electronic clinical thermometer having a store of plural temperature prediction functions in which elapsed measurement time is a variable, each function prescribing a temperature change up to a final, stable temperature. Temperature at a specific point in time is predicted in accordance with a selected temperature prediction function based on a temperature at a specific sampling instant and elapsed measurement time up to the past sampling instant. The predicted value and a value actually measured at the specific point in time are compared to obtain a difference between them. When the difference falls outside prescribed limits, another temperature prediction function is selected, a subsequent predicted value is obtained based thereon, and the foregoing process is repeated until a difference is found which lies within the prescribed limits. When this occurs, a predicted value of the final, stable temperature corresponding to the prediction function is obtained and delivered as an output.

摘要翻译： 一种具有多个温度预测功能的电子体温计，其中经过的测量时间是可变的，每个功能规定温度变化直到最终的稳定的温度。 根据特定采样时间的温度和直到过去采样时刻的经过的测量时间，根据所选择的温度预测函数预测特定时间点的温度。 将预测值和在特定时间点实际测量的值进行比较以获得它们之间的差异。 当差异超出规定限度时，选择另一温度预测函数，基于此获得随后的预测值，并且重复上述处理，直到发现位于规定限度内的差异。 当发生这种情况时，获得与预测函数相对应的最终稳定温度的预测值并将其输出作为输出。

公开(公告)号：US09006412B2

公开(公告)日：2015-04-14

申请号：US13122675

申请日：2009-10-05

申请人： Yoshikazu Fujii ,  Tadashi Fujii ,  Akira Arisawa ,  Tomohiro Tamura

发明人： Yoshikazu Fujii ,  Tadashi Fujii ,  Akira Arisawa ,  Tomohiro Tamura

IPC分类号： C07H21/04 ,  C12N15/00 ,  C12N15/64 ,  C12P21/06 ,  C12P21/02 ,  C12N9/02 ,  C12N15/74 ,  C12P7/02 ,  C12P7/62 ,  C12P17/06

CPC分类号： C12P21/02 ,  C12N9/0071 ,  C12N9/0073 ,  C12N15/74 ,  C12P7/02 ,  C12P7/62 ,  C12P17/06 ,  C12Y114/13013

摘要： An expression vector capable of expressing a foreign gene in Pseudonocardia autotrophica; a transformant of Pseudonocardia autotrophica produced by using the expression vector; a method for producing a protein by using the transformant; a method for producing an active form of vitamin D3 from vitamin D3, which comprises highly expressing a gene encoding an enzyme involved in the synthesis of the active form of vitamin D3 in a transformant by using the expression vector or the transformant; a method for producing 25-hydroxyvitamin D2 from vitamin D2; and a method for producing pravastatin from compactin, which comprises highly expressing a compactin hydroxylase gene in a transformant by using the expression vector or the transformant.

摘要翻译： 能够在自养假诺卡氏菌中表达外源基因的表达载体; 通过使用表达载体产生的假心内膜自养基因的转化体; 通过使用该转化体生产蛋白质的方法; 从维生素D3生产活性形式的维生素D3的方法，其包括通过使用表达载体或转化体高度表达编码参与转化体中活性形式的维生素D3的活性形式的酶的基因; 从维生素D2生产25-羟基维生素D 2的方法; 以及通过使用表达载体或转化体高效表达转化体中的致密蛋白羟化酶基因的来自美伐他汀的普伐他汀的制造方法。

公开(公告)号：US08460915B2

公开(公告)日：2013-06-11

申请号：US12449876

申请日：2008-02-28

申请人： Tadashi Fujii ,  Yoshikazu Fujii ,  Atsushi Ochiai ,  Masashi Ito ,  Kazuhiro Machida

发明人： Tadashi Fujii ,  Yoshikazu Fujii ,  Atsushi Ochiai ,  Masashi Ito ,  Kazuhiro Machida

IPC分类号： C12N9/00 ,  C12N9/02 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

CPC分类号： C12P7/18 ,  C12N9/0077

摘要： Disclosed is a means for improving the poor conversion efficiency in a bioconversion system using an Escherichia coli cell having a bacterium-originated cytochrome P-450 gene integrated therein. A recombinant Escherichia coli cell is produced by introducing aciB and aciC which encode a gene for the electron transport system originated from the Acinetobacter sp. OC4 strain into an Escherichia coli cell, and adding a polynucleotide encoding an N-terminal sequence composed of 48 amino acid residues of AciA and the like to the 5′-terminus of a bacterium-originated cytochrome P-450 gene, wherein AciA is an alkane-oxidative cytochrome P-450 originated from the Acinetobacter sp. OC4 strain. Use of the recombinant Escherichia coli cell results in much effective microbial conversion of a hydrophobic or amphipathic substrate compound into a desired compound.

摘要翻译： 公开了一种用于提高生物转化系统中转化效率差的方法，该系统使用其中整合有细菌色素细胞色素P-450基因的大肠杆菌细胞。 通过引入编码源自不动杆菌属的电子传递系统的基因的aciB和aciC来产生重组大肠杆菌细胞。 OC4菌株加入到大肠杆菌细胞中，并将编码由AciA等的48个氨基酸残基组成的N末端序列的多核苷酸加入到细菌来源的细胞色素P-450基因的5'末端，其中AciA为 链烷氧化细胞色素P-450源自不动杆菌属。 OC4菌株。 重组大肠杆菌细胞的使用导致疏水性或两亲性底物化合物成为所需化合物的有效微生物转化。

公开(公告)号：US20070026170A1

公开(公告)日：2007-02-01

申请号：US11489621

申请日：2006-07-20

申请人： Kenichi Kawano ,  Tadashi Fujii ,  Tatsuo Hiyoshi

发明人： Kenichi Kawano ,  Tadashi Fujii ,  Tatsuo Hiyoshi

IPC分类号： B41M5/40

CPC分类号： B41M5/506

摘要： A recording medium has an ink-receiving layer provided on at least one surface of a substrate, wherein the substrate comprises a base material, and a resin layer having ink absorbency and/or ink permeability formed at least on the side of the base material on which the ink-receiving layer is provided, and wherein the resin layer has a contact angle with respect to water of 60° or smaller, a water vapor transmission rate of 100 g/m2/24 hr or larger according to JIS-Z-0208 and the thickness of 10 to 100 μm.

摘要翻译： 记录介质具有设置在基板的至少一个表面上的油墨接收层，其中所述基板包括基材，以及至少在所述基材的侧面上形成油墨吸收性和/或油墨渗透性的树脂层， 其中设置有油墨接收层，并且其中树脂层相对于水的接触角为60°或更小，水蒸汽透过率为100g / m 2 / 24hr或 根据JIS-Z-0208较大，厚度为10〜100μm。

公开(公告)号：US07022502B2

公开(公告)日：2006-04-04

申请号：US10169257

申请日：2000-12-22

申请人： Tadashi Fujii ,  Yasuhide Aritoku ,  Manabu Mukaihara ,  Takao Narita ,  Hitosi Agematu ,  Kunio Isshiki

发明人： Tadashi Fujii ,  Yasuhide Aritoku ,  Manabu Mukaihara ,  Takao Narita ,  Hitosi Agematu ,  Kunio Isshiki

IPC分类号： C12P17/00

CPC分类号： C12Y105/01002 ,  C12N9/0026 ,  C12N9/0028 ,  C12P17/12

摘要： A process for the production of L-pipecolic acid which comprises the step of reducing delta-1-piperideine-6-carboxylic acid by the use of pyrroline-5-carboxylate reductase. The delta-1-piperideine-6-carboxylic acid is obtained by the step of converting L-lysine by the use of lysine 6-aminotransferase encoded by a gene of Flavobacterium lutescens. The steps of reducing delta-1-piperideine-6-carboxylic acid and the converting of L-lysine into L-pipecolic acid by the use of lysine 6-aminotransferase are carried out by using a bacterium transformed with a gene encoding lysine 6-aminotransferase wherein such bacterium comprises pyrroline-5-carboxylate reductase encoded by a gene of Escherichia coli or a coryneform bacterium. A recombinant bacterium which can be used in this production process is also provided. Thus, the present invention can provide an efficient biological process for the production of L-pipecolic acid (or 2-piperidinecarboxylic acid).

摘要翻译： 一种生产L-哌啶酸的方法，其包括通过使用吡咯啉-5-羧酸酯还原酶还原δ-1-哌啶-6-羧酸的步骤。 通过使用由黄杆菌（Flavobacterium lutescens）基因编码的赖氨酸6-氨基转移酶转化L-赖氨酸的步骤获得δ-1-哌啶-6-羧酸。 通过使用赖氨酸6-氨基转移酶减少δ-1-哌啶-6-羧酸和L-赖氨酸转化成L-哌啶酸的步骤是通过使用转染有编码赖氨酸6-氨基转移酶的基因的细菌 其中这种细菌包括由大肠杆菌或棒杆菌型细菌的基因编码的吡咯啉-5-羧酸还原酶。 还提供了可用于该生产方法的重组细菌。 因此，本发明可以提供生产L-哌啶酸（或2-哌啶羧酸）的有效的生物学方法。

公开(公告)号：US06890746B1

公开(公告)日：2005-05-10

申请号：US09762230

申请日：1999-08-04

申请人： Tadashi Fujii ,  Takao Narita ,  Kuniho Nakata ,  Hitosi Agematu ,  Hiroshi Tsunekawa ,  Kunio Isshiki ,  Takeo Yoshioka

发明人： Tadashi Fujii ,  Takao Narita ,  Kuniho Nakata ,  Hitosi Agematu ,  Hiroshi Tsunekawa ,  Kunio Isshiki ,  Takeo Yoshioka

IPC分类号： C12N1/21 ,  C12N9/00 ,  C12N9/02 ,  C12N9/10 ,  C12N15/53 ,  C12N15/54 ,  C12P13/14

CPC分类号： C12N9/0004 ,  C12N9/00 ,  C12N9/1096 ,  C12P13/14

摘要： Provided are an isolated gene capable of participating in the production of L-homoglutamic acid, and a production system of L-homoglutamic acid by using this gene. The gene is derived from the genome of Flavobacterium lutescens.

摘要翻译： 提供能够参与L-高谷氨酸生产的分离基因，以及使用该基因的L-高谷氨酸的生产体系​​。 该基因衍生自黄杆菌（Flavobacterium lutescens）的基因组。

公开(公告)号：US4701659A

公开(公告)日：1987-10-20

申请号：US777284

申请日：1985-09-18

申请人： Tadashi Fujii ,  Hiroyuki Yagami ,  Iwao Seo ,  Masahiro Sasaki

发明人： Tadashi Fujii ,  Hiroyuki Yagami ,  Iwao Seo ,  Masahiro Sasaki

IPC分类号： B06B1/06 ,  H04R17/00

CPC分类号： B06B1/0688 ,  Y10S310/80 ,  Y10T29/42 ,  Y10T29/49144

摘要： An ultrasonic transducer includes a piezoelectric member consisting of either a unpolarized piezoelectric polymer material or the like, or an in-advance polarized piezoelectric polymer material or the like, a first flexible substrate on which a first electrode set and their connecting leads are formed, these being bonded to one surface of the piezoelectric member, and a second flexible substrate on which a second electrode and its connecting lead are formed, these being bonded to the other surface of the piezoelectric member, wherein the piezoelectric member is sandwiched between the first electrode set and second electrode. The electrodes can apply a voltage to polarize the unpolarized piezoelectric polymer material in a manufacturing process of the piezoelectric member, or apply a drive voltage to the finished ultrasonic transducer. Also disclosed is a method of manufacturing the ultrasonic transducer.

摘要翻译： 超声波换能器包括由非极化压电聚合物材料等构成的压电元件或者先进的偏振压电聚合物材料等，其上形成有第一电极组及其连接引线的第一柔性基板，这些 被连接到压电元件的一个表面上，第二柔性基板上形成有第二电极及其连接引线，这些基板与压电元件的另一个表面接合，其中压电元件被夹在第一电极组 和第二电极。 电极可以在压电构件的制造过程中施加电压以使非极化的压电聚合物材料偏振，或者对最终的超声换能器施加驱动电压。 还公开了一种制造超声换能器的方法。