公开(公告)号：US20200208202A1

公开(公告)日：2020-07-02

申请号：US16812173

申请日：2020-03-06

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： Daniel T. Chiu ,  Jason E. Kreutz ,  Gloria S. Yen ,  Bryant S. Fujimoto

IPC: C12Q1/6816 ,  G06K9/46 ,  G06K9/00 ,  G01N21/64 ,  G01N21/47 ,  G06T7/62 ,  G01N15/14

Abstract: Methods, devices, and systems for performing digital assays are provided. In certain aspects, the methods, devices, and systems can be used for the amplification and detection of nucleic acids. In certain aspects, the methods, devices, and systems can be used for the recognition, detection, and sizing of droplets in a volume. Also provided are compositions and kits suitable for use with the methods and devices of the present disclosure.

公开(公告)号：US10590470B2

公开(公告)日：2020-03-17

申请号：US15962944

申请日：2018-04-25

Applicant: University of Washington Through Its Center for Commercialization

Inventor： Daniel T. Chiu ,  Bryant S. Fujimoto ,  Alexander R. Gansen ,  Gloria S. Yen ,  Robert M. Lorenz

IPC: C12Q1/68 ,  C12Q1/6851 ,  C12Q1/6806

Abstract: Methods, devices and systems for performing digital measurements are provided.

公开(公告)号：US10444243B2

公开(公告)日：2019-10-15

申请号：US15100056

申请日：2014-11-25

Applicant: University of Washington through its Center for Commercialization

Inventor： Daniel T. Chiu ,  Changfeng Wu ,  Jiangbo Yu

IPC: G01N33/58 ,  C09K11/06 ,  G01N21/64 ,  C09K11/02

Abstract: The present disclosure provides encoded chromophoric polymer particles that are capable of, for example, optical and/or biomolecular encoding of analytes. The present disclosure also provides suspensions comprising a plurality of encoded chromophoric polymer particles. The present disclosure also provides methods of using the encoded chromophoric polymer particles and systems for performing multiplex analysis with encoded chromophoric polymer particles.

公开(公告)号：US12216124B2

公开(公告)日：2025-02-04

申请号：US18058458

申请日：2022-11-23

Applicant: University of Washington through its Center for Commercialization

Inventor： Daniel T. Chiu ,  Changfeng Wu ,  Xuanjun Zhang ,  Jiangbo Yu ,  Fangmao Ye

IPC: C09K11/06 ,  B82Y15/00 ,  B82Y30/00 ,  B82Y40/00 ,  C08G61/12 ,  C08J3/11 ,  C09K11/02 ,  G01N33/58 ,  H10K85/10

Abstract: The present invention provides, among other aspects, stabilized chromophoric nanoparticles. In certain embodiments, the chromophoric nanoparticles provided herein are rationally functionalized with a pre-determined number of functional groups. In certain embodiments, the stable chromophoric nanoparticles provided herein are modified with a low density of functional groups. In yet other embodiments, the chromophoric nanoparticles provided herein are conjugated to one or more molecules. Also provided herein are methods for making rationally functionalized chromophoric nanoparticles.

公开(公告)号：US20240254288A1

公开(公告)日：2024-08-01

申请号：US18317288

申请日：2023-05-15

Applicant: University of Washington through its Center for Commercialization

Inventor： Daniel T. Chiu ,  Changfeng Wu ,  Yu Rong ,  Yong Zhang ,  Yi-Che Wu ,  Yang-Hsiang Chan ,  Xuanjun Zhang ,  Jiangbo Yu ,  Wei Sun

IPC: C08G75/32 ,  A61K49/00 ,  B82Y30/00 ,  B82Y40/00 ,  C08G79/00 ,  C09B69/10 ,  G01N21/64 ,  G01N33/58 ,  H10K50/11 ,  H10K85/10

CPC classification number: C08G75/32 ,  A61K49/0019 ,  A61K49/0067 ,  C08G79/00 ,  C09B69/105 ,  G01N33/582 ,  G01N33/587 ,  H10K85/10 ,  H10K85/113 ,  H10K85/115 ,  H10K85/151 ,  B82Y30/00 ,  B82Y40/00 ,  G01N2021/6439 ,  H10K50/11

Abstract: Polymers, monomers, chromophoric polymer dots and related methods are provided. Highly fluorescent chromophoric polymer dots with narrow-band emissions are provided. Methods for synthesizing the chromophoric polymers, preparation methods for forming the chromophoric polymer dots, and biological applications using the unique properties of narrow-band emissions are also provided.

公开(公告)号：US11982678B2

公开(公告)日：2024-05-14

申请号：US16171918

申请日：2018-10-26

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： Daniel T. Chiu ,  Perry G. Schiro ,  Jason S. Kuo

IPC: G01N33/58 ,  G01N21/25 ,  G01N22/00 ,  G01N33/53 ,  G01N33/574 ,  G01N33/68 ,  G01N15/1433 ,  G01N21/64

CPC classification number: G01N33/6893 ,  G01N21/25 ,  G01N22/00 ,  G01N33/5302 ,  G01N33/5308 ,  G01N33/57407 ,  G01N33/582 ,  G01N15/1433 ,  G01N21/64 ,  Y02A50/30

Abstract: Provided herein, among other aspects, are methods and apparatuses for ranking aliquots from a suspension containing bioparticles. In certain embodiments, the bioparticles may be cells, organelles, proteins, DNAs, debris of biological origin, microbeads coated with biological compounds, or viral particles. As such, the methods and apparatuses provided herein may be used to quantify rare cells such as circulating cancer cells, fetal cells and other rare cells present in bodily fluids for disease diagnosis, prognosis, or treatment.

公开(公告)号：US11939626B2

公开(公告)日：2024-03-26

申请号：US17813415

申请日：2022-07-19

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： Daniel T. Chiu ,  Jason E. Kreutz ,  Gloria S. Yen ,  Bryant S. Fujimoto

IPC: C12Q1/6816 ,  G01N15/14 ,  G01N15/1434 ,  G01N21/47 ,  G01N21/64 ,  G06T7/62 ,  G06V20/69

CPC classification number: C12Q1/6816 ,  G01N15/1434 ,  G01N21/47 ,  G01N21/6428 ,  G06T7/62 ,  G06V20/695 ,  G01N2015/1445 ,  G01N2021/6439 ,  G06T2207/10056 ,  G06T2207/30072 ,  C12Q1/6816 ,  C12Q2531/113 ,  C12Q2537/157 ,  C12Q2563/107 ,  C12Q2563/159

Abstract: Methods, devices, and systems for performing digital assays are provided. In certain aspects, the methods, devices, and systems can be used for the amplification and detection of nucleic acids. In certain aspects, the methods, devices, and systems can be used for the recognition, detection, and sizing of droplets in a volume. Also provided are compositions and kits suitable for use with the methods and devices of the present disclosure.

公开(公告)号：US20220082566A1

公开(公告)日：2022-03-17

申请号：US17538447

申请日：2021-11-30

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： Daniel T. Chiu ,  Changfeng Wu ,  Jiangbo Yu

IPC: G01N33/58 ,  C09K11/06 ,  G01N21/64 ,  C09K11/02

Abstract: The present disclosure provides encoded chromophoric polymer particles that are capable of, for example, optical and/or biomolecular encoding of analytes. The present disclosure also provides suspensions comprising a plurality of encoded chromophoric polymer particles. The present disclosure also provides methods of using the encoded chromophoric polymer particles and systems for performing multiplex analysis with encoded chromophoric polymer particles.

公开(公告)号：US11221336B2

公开(公告)日：2022-01-11

申请号：US16556006

申请日：2019-08-29

Applicant: UNIVERSITY OF WASHINGTON THROUGH ITS CENTER FOR COMMERCIALIZATION

Inventor： Daniel T. Chiu ,  Changfeng Wu ,  Jiangbo Yu

IPC: G01N33/58 ,  C09K11/06 ,  G01N21/64 ,  C09K11/02

Abstract: The present disclosure provides encoded chromophoric polymer particles that are capable of, for example, optical and/or biomolecular encoding of analytes. The present disclosure also provides suspensions comprising a plurality of encoded chromophoric polymer particles. The present disclosure also provides methods of using the encoded chromophoric polymer particles and systems for performing multiplex analysis with encoded chromophoric polymer particles.

公开(公告)号：US20200371109A1

公开(公告)日：2020-11-26

申请号：US16989491

申请日：2020-08-10

Applicant: University of Washington through its Center for Commercialization

Inventor： Daniel T. Chiu ,  Changfeng Wu ,  Xuanjun Zhang ,  Jiangbo Yu ,  Fangmao Ye

IPC: G01N33/58 ,  B82Y30/00 ,  B82Y40/00 ,  C08G61/12 ,  C08J3/11 ,  C09K11/02 ,  C09K11/06 ,  H01L51/00 ,  B82Y15/00

Abstract: The present invention provides, among other aspects, stabilized chromophoric nanoparticles. In certain embodiments, the chromophoric nanoparticles provided herein are rationally functionalized with a pre-determined number of functional groups. In certain embodiments, the stable chromophoric nanoparticles provided herein are modified with a low density of functional groups. In yet other embodiments, the chromophoric nanoparticles provided herein are conjugated to one or more molecules. Also provided herein are methods for making rationally functionalized chromophoric nanoparticles.