公开(公告)号：US07501276B2

公开(公告)日：2009-03-10

申请号：US10202419

申请日：2002-07-23

申请人： Werner Baumann ,  Ralf Ehret ,  Mirko Lehmann ,  Günter Igel ,  Hans-Jürgen Gahle ,  Ulrich Sieben ,  Ingo Freund ,  Martin Brischwein

发明人： Werner Baumann ,  Ralf Ehret ,  Mirko Lehmann ,  Günter Igel ,  Hans-Jürgen Gahle ,  Ulrich Sieben ,  Ingo Freund ,  Martin Brischwein

IPC分类号： C12M1/42 ,  C12N15/87

CPC分类号： C12N15/89 ,  C12M35/02 ,  C12M35/04 ,  C12M35/08 ,  C12N13/00

摘要： A process is provided for the intracellular manipulation of a biological cell (3) which is positioned adhering to a support area (5) in a culture medium (2). Inside the support area (5) for the cell (3) an opening into the membrane of the cell (3) is created spaced from its support edge. The edge of the cell membrane surrounding the opening, adhering to the support area (5), thus seals off the cell fluid situated in the interior of the cell (3) from the culture medium (2) and insulates the cell fluid against the culture medium (2). The interior of the cell (3) is manipulated through the opening. An apparatus for implementing the process is also provided, including an object carrier (4) with a support area (5) for adhering the cell and a poration tool (6) for creating the opening in the cell membrane. The poration tool (6) may be any of various chemical, mechanical and/or electrical devices.

摘要翻译： 提供了一种用于细胞内操作生物细胞（3）的方法，所述生物细胞（3）定位为粘附在培养基（2）中的支持区域（5）。 在电池（3）的支撑区域（5）的内部，与电池（3）的隔膜形成的开口与其支撑边缘间隔开。 围绕开口的细胞膜的边缘附着在支撑区域（5）上，从而将位于细胞（3）内部的细胞液体与培养基（2）密封在一起，并使细胞液体抵抗培养物 中等（2）。 电池（3）的内部通过开口被操纵。 还提供了一种用于实现该方法的装置，包括具有用于粘附细胞的支撑区域（5）的物体载体（4）和用于在细胞膜中形成开口的引锭工具（6）。 引锭工具（6）可以是各种化学，机械和/或电气装置中的任何一种。

公开(公告)号：US20060233662A1

公开(公告)日：2006-10-19

申请号：US10569329

申请日：2004-08-19

申请人： Mirko Lehmann ,  Ulrich Sieben

发明人： Mirko Lehmann ,  Ulrich Sieben

IPC分类号： G01N31/22

CPC分类号： B01L3/502707 ,  B01J2219/00378 ,  B01J2219/00527 ,  B01J2219/00545 ,  B01J2219/00549 ,  B01J2219/00576 ,  B01J2219/00578 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00704 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L2300/0636 ,  B01L2300/0654 ,  C40B40/06 ,  C40B40/10 ,  G01N21/78 ,  G01N33/54366

摘要： In a carrier medium having at least two defined regions, biological and/or chemical substances are applied to the regions of the carrier medium for analyzing an analyte. The carrier medium is also provided with a stored code that indicates which biological and/or chemical substance is located in which defined region. The application of multiple biological and/or chemical substances to one carrier medium enables multiple analyses to be performed simultaneously on the analyte. This approach reduces the required quantities of the biological and/or chemical substances needed to perform the desired analyses of the analyte. The carrier medium itself does not reveal which biological and/or chemical substance is located in which region; this information may instead be provided by the code.

摘要翻译： 在具有至少两个限定区域的载体介质中，将生物和/或化学物质施加到载体介质的区域以分析分析物。 载体介质还具有指示哪个生物和/或化学物质位于哪个限定区域的存储代码。 将多种生物和/或化学物质应用于一种载体介质使得能够在分析物上同时进行多次分析。 该方法减少了对分析物进行所需分析所需的生物和/或化学物质所需的量。 载体介质本身不显露哪个生物和/或化学物质位于哪个区域; 该信息可以由代码提供。

公开(公告)号：US06638743B2

公开(公告)日：2003-10-28

申请号：US10119354

申请日：2002-04-09

申请人： Werner Baumann ,  Ralf Ehret ,  Mirko Lehmann ,  Günter Igel ,  Hans-Jörg Gahle ,  Bernhard Wolf ,  Ulrich Sieben ,  Ingo Freund ,  Martin Brischwein

发明人： Werner Baumann ,  Ralf Ehret ,  Mirko Lehmann ,  Günter Igel ,  Hans-Jörg Gahle ,  Bernhard Wolf ,  Ulrich Sieben ,  Ingo Freund ,  Martin Brischwein

IPC分类号： C12N1300

CPC分类号： C12N15/89 ,  C12M35/02 ,  C12M35/04 ,  C12M35/08 ,  C12N13/00

摘要： A method is provided for measuring a state variable of a biological cell (3) located in a nutrient medium (2) and supported on and adhering to a support area (5). Within the support area (5) for the cell (3) and at a distance from the support area edge, an opening is made in the membrane of the cell (3). The edge of the cell membrane that surrounds the opening and adheres to the support area (5) seals off the liquid found inside the cell (3) from the nutrient medium (2). Through the opening the state variable (2) is measured. An apparatus for performing the method is also provided.

公开(公告)号：US06475760B1

公开(公告)日：2002-11-05

申请号：US09321051

申请日：1999-05-27

申请人： Werner Baumann ,  Ralf Ehret ,  Mirko Lehmann ,  Günter Igel ,  Hans-Jürgen Gahle ,  Bernhard Wolf ,  Ulrich Sieben ,  Ingo Freund ,  Martin Brischwein

发明人： Werner Baumann ,  Ralf Ehret ,  Mirko Lehmann ,  Günter Igel ,  Hans-Jürgen Gahle ,  Bernhard Wolf ,  Ulrich Sieben ,  Ingo Freund ,  Martin Brischwein

IPC分类号： C12N1300

CPC分类号： C12N15/89 ,  C12M35/02 ,  C12M35/04 ,  C12M35/08 ,  C12N13/00

摘要： A process is provided for the intracellular manipulation of a biological cell (3) which is positioned adhering to a support area (5) in a culture medium (2). Inside the support area (5) for the cell (3) an opening into the membrane of the cell (3) is created spaced from its support edge. The edge of the cell membrane surrounding the opening, adhering to the support area (5), thus seals off the cell fluid situated in the interior of the cell (3) from the culture medium (2) and insulates the cell fluid against the culture medium (2). The interior of the cell (3) is manipulated through the opening. An apparatus for implementing the process is also provided, including an object carrier (4) with a support area (5) for adhering the cell and a poration tool (6) for creating the opening in the cell membrane. The poration tool (6) may be any of various chemical, mechanical and/or electrical devices.

摘要翻译： 提供了一种用于细胞内操作生物细胞（3）的方法，所述生物细胞（3）定位为粘附在培养基（2）中的支持区域（5）。 在电池（3）的支撑区域（5）的内部，与电池（3）的隔膜形成的开口与其支撑边缘间隔开。 围绕开口的细胞膜的边缘附着在支撑区域（5）上，从而将位于细胞（3）内部的细胞液体与培养基（2）密封并使细胞液体抵抗培养物 中等（2）。 电池（3）的内部通过开口被操纵。 还提供了一种用于实现该方法的装置，包括具有用于粘附细胞的支撑区域（5）的物体载体（4）和用于在细胞膜中形成开口的引锭工具（6）。 引锭工具（6）可以是各种化学，机械和/或电气装置中的任何一种。

公开(公告)号：US08084792B2

公开(公告)日：2011-12-27

申请号：US12840597

申请日：2010-07-21

申请人： Mirko Lehmann ,  Ingo Freund

发明人： Mirko Lehmann ,  Ingo Freund

IPC分类号： H01L29/78

CPC分类号： G01N27/4148

摘要： An electric component comprising a sensor and/or actuator chip with a substrate on which a passivating layer and a sensor and/or actuator structure consisting of an active surface area is arranged. The chip is surrounded by an encapsulation having an opening which forms an access to the at least one active surface area. A layer stack is arranged on the substrate, said stack of layers comprising from the passivating layer to the substrate at least one first strip conductor layer, a first electric insulating layer, a second strip conductor layer and a second electric insulating layer. The first conductor strip layer is fully arranged outside the area of the chip covered by the opening. At least one conductor strip of the second conductor strip layer is connected to the sensor and/or actuator structure.

摘要翻译： 一种电气部件，包括具有衬底的传感器和/或致动器芯片，其上布置有由活性表面积组成的钝化层和传感器和/或致动器结构。 芯片由具有开口的封装围绕，该开口形成对至少一个有源表面区域的访问。 层叠在衬底上，层叠层包括至少一个第一带状导体层，第一电绝缘层，第二带状导体层和第二电绝缘层，从钝化层到衬底。 第一导体带层完全布置在由开口覆盖的芯片的区域的外部。 第二导体带层的至少一个导体条连接到传感器和/或致动器结构。

公开(公告)号：US07592195B2

公开(公告)日：2009-09-22

申请号：US10504738

申请日：2003-02-07

申请人： Markus Rogalla ,  Ingo Freund ,  Mirko Lehmann

发明人： Markus Rogalla ,  Ingo Freund ,  Mirko Lehmann

IPC分类号： H01L21/00

CPC分类号： B81B7/0061

摘要： In a method for producing a sensor arrangement and the resulting sensor arrangement, a sensor is provided on or in a chip and the chip is covered with a protective cover, the cover being an interface between the sensor and the environment. An adhesive layer is provided between the chip and the protective cover, the adhesive layer alone or together with the protective cover being an interface between the sensor and the environment. The protective cover and/or the adhesive layer may have a channel formed therein, the channel functioning as the reception channel for a sensor. In an alternative embodiment, the protective cover placed on a wafer with several chips, and the wafer is cut up to produce the individual chips with the protective cover. Thus, a sensor arrangement may have the protective cover applied to the individual chip after the chip is cut from the wafer, or the protective cover may be applied to the wafer, and the wafer and cover are then cut up into the individual chips and corresponding covers. The channel leading from one side of the arrangement to the sensor may be taken through the adhesive layer, through the protective cover, or through both. A hole may be formed in the protective cover above the sensor, with the sensor lying loosely in the hole. The reaction volume may be determined by the dimensions of the hole.

摘要翻译： 在用于制造传感器装置和所得到的传感器装置的方法中，传感器设置在芯片上或芯片中，并且芯片被保护盖覆盖，盖是传感器和环境之间的接口。 粘合剂层设置在芯片和保护盖之间，粘合剂层单独或与保护罩一起作为传感器和环境之间的界面。 保护盖和/或粘合剂层可以具有形成在其中的通道，该通道用作传感器的接收通道。 在替代实施例中，将保护盖放置在具有几个芯片的晶片上，并且切割晶片以用保护盖产生单独的芯片。 因此，在从晶片切割芯片之后，传感器装置可以具有施加到单个芯片的保护盖，或者可以将保护盖施加到晶片，然后将晶片和盖切割成各个芯片，并且对应于 盖子。 从布置的一侧引导到传感器的通道可以穿过粘合剂层，通过保护盖，或者通过两者。 可以在传感器上方的保护罩中形成孔，传感器松动地位于孔中。 反应体积可以由孔的尺寸确定。

公开(公告)号：US07554135B2

公开(公告)日：2009-06-30

申请号：US10592848

申请日：2005-03-04

申请人： Mirko Lehmann ,  Heinz-Peter Frerichs ,  Christoph Wilbertz

发明人： Mirko Lehmann ,  Heinz-Peter Frerichs ,  Christoph Wilbertz

IPC分类号： H01L23/58

CPC分类号： G01N27/4143 ,  H01L2924/0002 ,  H01L2924/00

摘要： A device for detecting a gas or gas mixture having at least one first gas sensor designed as an SGFET and at least—one second, additional gas sensor designed as a Lundström-FET. The gas sensors are connected to a processing device designed to analyze the measurement signals from both types of gas sensors in order to detect the gas or gas mixture.

摘要翻译： 一种用于检测气体或气体混合物的装置，其具有设计为SGFET的至少一个第一气体传感器和设计为Lundström-FET的至少一个第二附加气体传感器。 气体传感器连接到设计用于分析来自两种类型的气体传感器的测量信号的处理装置，以便检测气体或气体混合物。

公开(公告)号：US07422816B2

公开(公告)日：2008-09-09

申请号：US11074594

申请日：2005-03-08

申请人： Gilbert Erdler ,  Mirko Lehmann ,  Class Müller

发明人： Gilbert Erdler ,  Mirko Lehmann ,  Class Müller

IPC分类号： H01M2/00

CPC分类号： H01M8/16 ,  H01M8/186 ,  H01M14/005 ,  Y02E60/527 ,  Y02E60/528

摘要： A fuel cell system includes a fuel cell and a fuel infeed device. The fuel cell has first and second electrodes (cathode and anode). A layer with catalytic activity permeable to protons is between the electrodes. A fuel supply device provides a fuel, and a reactant supply device provides a reactant that reacts with protons from the fuel to produce current. The fuel and reactant supply devices are located directly on the face of an electrode. The fuel infeed device is connected to the fuel supply device for feeding in the fuel, the fuel supply device being one of the electrodes. The fuel supply device also stores the fuel. Besides supplying an electrical circuit with the fuel cell system as a micro energy supply device, the fuel cell system as a fuel infeed device also has an integrated electrolysis unit for regenerating the fuel supply.

摘要翻译： 燃料电池系统包括燃料电池和燃料供给装置。 燃料电池具有第一和第二电极（阴极和阳极）。 具有对质子可渗透的催化活性的层位于电极之间。 燃料供应装置提供燃料，并且反应物供应装置提供与来自燃料的质子反应以产生电流的反应物。 燃料和反应物供应装置直接位于电极的表面上。 燃料供给装置连接到用于供给燃料的燃料供给装置，燃料供给装置是电极之一。 燃料供给装置还存储燃料。 除了将燃料电池系统的电路作为微能量供给装置提供之外，作为燃料供给装置的燃料电池系统还具有用于再生燃料供应的集成电解单元。

公开(公告)号：US20070105111A1

公开(公告)日：2007-05-10

申请号：US10564172

申请日：2004-06-30

申请人： Mirko Lehmann

发明人： Mirko Lehmann

IPC分类号： C12Q1/68

CPC分类号： B29C59/18 ,  B01L3/508 ,  B29C70/64

摘要： In a method for structuring the surface of a substrate, a substrate is elastically expanded by the application of a tensile stress so that a surface area of the substrate in which a structure is to be created, is enlarged. Then, in the surface area, a structure is produced that is larger than the structure to be produced. For this purpose at least one solution is applied to the substrate, which solvent contains at least one solid substance dissolved in a solvent. The solvent is then removed from the surface of the substrate so that the solid substance remains behind. The expansion of the substrate is at least partly reversed by the reduction or removal of the tensile stress so that the size of the structure is reduced to the size of the structure to be produced. In the material of the substrate, a compression stress can also be created to reduce the size of the structure to the size of the structure to be produced.

摘要翻译： 在用于构造基板的表面的方法中，通过施加拉伸应力使基板弹性膨胀，从而扩大了其中将产生结构的基板的表面积。 然后，在表面积中产生比要制造的结构大的结构。 为此目的，将至少一种溶液应用于底物，该溶剂含有溶解在溶剂中的至少一种固体物质。 然后从基材的表面除去溶剂，使得固体物质保留在后面。 通过拉伸应力的减少或去除，基材的膨胀至少部分地反转，使得结构的尺寸减小到要生产的结构的尺寸。 在基材的材料中，还可以产生压缩应力以将结构的尺寸减小到待生产的结构的尺寸。

公开(公告)号：US06191489B1

公开(公告)日：2001-02-20

申请号：US09506634

申请日：2000-02-18

申请人： Günter Igel ,  Hans-Jürgen Gahle ,  Mirko Lehmann

发明人： Günter Igel ,  Hans-Jürgen Gahle ,  Mirko Lehmann

IPC分类号： H01L2348

CPC分类号： H01L24/12 ,  H01L23/3192 ,  H01L24/11 ,  H01L2224/13018 ,  H01L2224/13022 ,  H01L2224/13099 ,  H01L2224/13144 ,  H01L2224/13147 ,  H01L2924/01004 ,  H01L2924/01005 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01022 ,  H01L2924/01029 ,  H01L2924/01033 ,  H01L2924/01046 ,  H01L2924/01047 ,  H01L2924/01049 ,  H01L2924/01074 ,  H01L2924/01078 ,  H01L2924/01079 ,  H01L2924/01082 ,  H01L2924/014 ,  H01L2924/14 ,  H01L2924/19043 ,  H01L2924/351 ,  H01L2924/00

摘要： A process is provided for manufacturing a layer arrangement (1) having a bump for a flip chip or similar connection. The layer arrangement has a plurality of layers (2, 3, 4, 5, 6, 7, 11) made of solid material and stacked into a layer stack (8). A recess (10) that extends over several layers (2, 3, 4, 5, 6, 7, 11) is made in the layer stack (8) transverse to the coating planes of the layers (2, 3, 4, 5, 6, 7, 11). A bump material (14) is placed in the recess (10). A profiling is created on the lateral boundary wall of the recess (10) by removal of layer material of different layers (2, 3, 4, 5, 6, 7, 11) of the layer stack (8). The profiling, starting from the surface (9) of the layer stack (8) and progressing in layers to the inside of the recess (10), has at least two indentations (12) and at least one projection (13) located between them. After the production of the profiling, a bump material (14) is brought into the recess (10) in such a way that it grasps behind the indentations (12).

摘要翻译： 提供了一种用于制造具有用于倒装芯片或类似连接的凸块的层布置（1）的工艺。 层布置具有由固体材料制成并堆叠成层堆叠（8）的多个层（2,3,4,5,6,7,11）。 在层叠体（8）中横跨多层（2,3,4,5,6,7,11）的层（2,3,4,5,11）的涂层平面（2,3,4,5） ，6,7,11）。 凸起材料（14）放置在凹部（10）中。 通过去除层叠（8）的不同层（2,3,4,5,6,7,11）的层材料，在凹槽（10）的侧边界壁上形成轮廓。 从层叠体（8）的表面（9）开始并且分层进入凹部（10）的内部的成型具有至少两个凹口（12）和位于它们之间的至少一个突起（13） 。 在生产轮廓之后，将凸块材料（14）以这样一种方式被带到凹槽（10）中，使得它抓住压痕（12）的后面。