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公开(公告)号：US08062843B2
公开(公告)日：2011-11-22
申请号：US12480981
申请日：2009-06-09
CPC分类号： C12N15/1013 , C12N15/1003 , C12Q1/6806 , C12Q2527/125
摘要： The invention provides the use of water-miscible liquid cyclic acetals for adsorbing a nucleic acid to a solid phase such as a substrate with a silica surface. To this end, the invention also provides compositions comprising said acetals. Methods are disclosed and claimed to purify nucleic acids from samples, as well as kits useful for performing these methods. The nucleic acids purified by a method of the invention are suited for assays aiming at the detection of a target nucleic acid.
摘要翻译： 本发明提供了水混溶性液体环状缩醛用于将核酸吸附到固相（例如具有二氧化硅表面的基底）的用途。 为此，本发明还提供包含所述缩醛的组合物。 方法被公开并要求从样品中纯化核酸，以及用于实施这些方法的试剂盒。 通过本发明的方法纯化的核酸适合用于检测靶核酸的测定。
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22.发明授权Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification 有权苯并咪唑鎓化合物和苯并咪唑化合物的盐用于核酸扩增公开(公告)号：US07781165B2
公开(公告)日：2010-08-24
申请号：US11581735
申请日：2006-10-16
IPC分类号： C12Q1/68 , C12P19/34 , C07D235/00
CPC分类号： C12Q1/6844 , C12Q2527/125
摘要： The present invention is directed to the use of a benzimidazolium compound comprising a side chain at at least one of its N-residues, said chain being either a Cn-alkyl or a substituted Cn-alkyl, characterized in that preferably n is ≧3, as an additive for a nucleic acid amplification reaction.
摘要翻译： 本发明涉及在其N-残基中的至少一个上含有侧链的苯并咪唑化合物的用途，所述链是C 1 - 烷基或取代的C n - 烷基，其特征在于n优选≥3， 作为核酸扩增反应的添加剂。
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公开(公告)号：US07759470B2
公开(公告)日：2010-07-20
申请号：US11707399
申请日：2007-02-16
申请人： Dieter Heindl , Frank Bergmann , Hans-Peter Josel , Dirk Kessler , Gunter Lampert , Christian Weilke
发明人： Dieter Heindl , Frank Bergmann , Hans-Peter Josel , Dirk Kessler , Gunter Lampert , Christian Weilke
摘要： The current invention restates substituted indole nucleosides as both terminal as well as internal building blocks of labeled oligonucleotide probes for the detection, analysis and quantitation of nucleic acids. The substituent comprises a linker and a detectable group or a linker and a reactive group for post synthesis coupling. These modified nucleosides grant access to a wide application area. These new substituted indole nucleosides can be used as labeling reagents for the facile preparation of e.g. optimized hybridization probes, Taqman-probes, or molecular beacon probes.
摘要翻译： 本发明将取代的吲哚核苷作为两个末端以及用于核酸检测，分析和定量的标记的寡核苷酸探针的内部构建块重述。 取代基包括接头和可检测的基团或接头以及用于后合成偶联的反应性基团。 这些修饰的核苷允许进入广泛的应用领域。 这些新的取代的吲哚核苷可以用作标记试剂，用于容易地制备例如 优化的杂交探针，Taqman探针或分子信标探针。
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24.发明授权N8- and C8-linked purine bases and structurally related heterocycles as universal nucleosides used for oligonucleotides hybridization 有权N8和C8连接的嘌呤碱基和结构相关杂环作为用于寡核苷酸杂交的通用核苷公开(公告)号：US07566775B2
公开(公告)日：2009-07-28
申请号：US11338149
申请日：2006-01-23
摘要： The present invention is directed to a nucleic acid binding compound comprising N8- or C8-linked purine bases or structurally related heterocycles, a compound useful for the preparation of such compound, a binding product of this nucleic acid binding compound with a nucleic acid, a method for the determination of a nucleic acid using said compound, and several uses of 8-linked purine bases and structurally related heterocycles. Compounds according to the present invention have advantageous properties when used in hybridization methods.
摘要翻译： 本发明涉及包含N8-或C8-连接的嘌呤碱基或结构相关杂环的核酸结合化合物，可用于制备该化合物的化合物，该核酸结合化合物与核酸的结合产物， 使用所述化合物测定核酸的方法以及8-连接的嘌呤碱基和结构相关杂环的几种用途。 当用于杂交方法时，根据本发明的化合物具有有利的性质。
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公开(公告)号：US07276592B2
公开(公告)日：2007-10-02
申请号：US10816298
申请日：2004-04-01
CPC分类号： A61K31/513 , A61K31/52 , A61K31/675 , C07D405/04 , C07D495/04 , C07H19/06 , C07H19/10 , C07H19/16 , C07H21/00 , C07H21/04 , C12P19/34 , C12P19/38 , C12Q1/68 , G01N33/53 , G01N33/566 , G01N33/58 , G01N37/00 , Y02P20/55
摘要： The present invention is related to compounds comprising six-membered rings capable of being incorporated into nucleic acids. Particularly, the six-membered ring is a derivative of cyclohexane, cyclohexene, tetrahydropyran, tetrahydrothiopyran or piperidine. These compounds may be used to build up oligomeric compounds. The invention is further related to uses of these oligomeric compounds for hybridization and as probes. In addition, methods for the detection of nucleic acids are provided wherein the oligomeric compounds are used.
摘要翻译： 本发明涉及能够并入核酸中的包含六元环的化合物。 特别地，六元环是环己烷，环己烯，四氢吡喃，四氢噻喃或哌啶的衍生物。 这些化合物可用于构建低聚化合物。 本发明还涉及这些寡聚化合物用于杂交和作为探针的用途。 此外，提供了检测核酸的方法，其中使用低聚化合物。
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公开(公告)号：US06929697B2
公开(公告)日：2005-08-16
申请号：US10503871
申请日：2003-02-20
CPC分类号： C23C2/24
摘要： The invention relates to a device for hot dip coating metal strand (1), particularly strip steel, in which the metal strand (1) can be vertically guided through a reservoir (3), which accommodates the molten coating metal (2), and though a guide channel (4) connected upstream therefrom. An electromagnetic inductor (5) is mounted in the area of the guide channel (4) and in order to retain the coating metal (2) inside the reservoir (3), can induce induction currents in the coating metal (2) by an electromagnetic traveling field. While interacting with the electromagnetic traveling field, the induction currents exert an electromagnetic force. The inductor (5) has at least two main coils (6) that are arranged in succession in movement direction (X) of the metal strand (1), and has at least two correction coils (7) for controlling the position of the metal strand (1) inside the guide channel (4) in direction (N), which is normal to the surface of the metal strand (1). These correction coils are also arranged in succession in movement direction (X) of the metal strand (1). In order to improve the efficiency of the control of the metal strip inside the guide channel, the invention provides that at least a portion of the correction coils (7), when viewed in movement direction (X) of the metal strand (1), are arranged so that they are offset with regard to one another perpendicular to movement direction (X) and perpendicular to direction (N) that is normal to the surface of the metal strand (1).
摘要翻译： 本发明涉及一种用于热浸镀金属线（1），特别是带钢的装置，其中金属股线（1）可以垂直地引导通过容纳熔融涂层金属（2）的储存器（3），以及 尽管其上游连接有引导通道（4）。 电磁感应器（5）安装在引导通道（4）的区域中，为了将涂层金属（2）保持在储存器（3）内部，可以通过电磁感应器（2）诱导涂层金属（2）中的感应电流 旅游领域。 当与电磁行进场相互作用时，感应电流施加电磁力。 电感器（5）具有至少两个主要线圈（6），它们沿金属线束（1）的移动方向（X）连续布置，并且具有至少两个用于控制金属线的位置的校正线圈（7） 在导向通道（4）内的方向（N）上的线（1），其垂直于金属线（1）的表面。 这些校正线圈也在金属线（1）的移动方向（X）上相继排列。 为了提高引导通道内的金属带的控制效率，本发明提供了当从金属线（1）的移动方向（X）观察时，至少一部分校正线圈（7） 被布置成使得它们相对于垂直于运动方向（X）并且垂直于垂直于金属线（1）的表面的方向（N）彼此偏移。
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公开(公告)号：US20120164643A1
公开(公告)日：2012-06-28
申请号：US13361549
申请日：2012-01-30
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6841 , C12Q1/6876 , C12Q1/6886 , C12Q2565/1025 , C12Q2563/107
摘要： The present disclosure relates to a set of at least 100 single-stranded oligonucleotide probes directed against (or complementary to) portions of a genomic target sequence of interest. The present disclosure also relates to a method of detecting a genomic target sequence of interest using the set of oligonucleotide probes and a method of generating the set of oligonucleotide probes. Further, the present disclosure relates to a kit comprising the set of oligonucleotide probes and at least one further component.
摘要翻译： 本公开内容涉及针对感兴趣的基因组靶序列的（或互补）部分的一组至少100个单链寡核苷酸探针。 本公开还涉及使用该组寡核苷酸探针检测感兴趣的基因组靶序列的方法和产生寡核苷酸探针组的方法。 此外，本公开涉及包含该组寡核苷酸探针和至少一种另外的组分的试剂盒。
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![Nucleic acid binding compounds containing pyrazolo[3,4-D]pyrimidine analogues of purin-2,6-diamine and their uses](/abs-image/US/2011/11/15/US08057997B2/abs.jpg.150x150.jpg)
28.发明授权Nucleic acid binding compounds containing pyrazolo[3,4-D]pyrimidine analogues of purin-2,6-diamine and their uses 有权含有嘌呤-2,6-二胺的吡唑并[3,4-D]嘧啶类似物的核酸结合化合物及其用途公开(公告)号：US08057997B2
公开(公告)日：2011-11-15
申请号：US11742416
申请日：2007-04-30
申请人： Frank Seela , Frank Bergmann , Herbert Von Der Eltz , Dieter Heindl , Christoph Seidel , Georg Becher
发明人： Frank Seela , Frank Bergmann , Herbert Von Der Eltz , Dieter Heindl , Christoph Seidel , Georg Becher
CPC分类号： C07H21/00 , C07H19/16 , Y10T436/143333
摘要： The present invention is in the field of nucleic acid binding compounds comprising 7-substituted 7-deaza-8aza-2,6-diamino-purine bases, compounds useful for the preparation of such compounds, various uses thereof and methods for the determination of nucleic acids using said compounds in the field of diagnostics.
摘要翻译： 本发明涉及包含7-取代的7-脱氮-8-氮杂-2,6-二氨基嘌呤碱基的核酸结合化合物领域，可用于制备这些化合物的化合物，其各种用途和用于测定核酸的方法 在诊断领域使用所述化合物的酸。
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公开(公告)号：US20110266172A1
公开(公告)日：2011-11-03
申请号：US13182691
申请日：2011-07-14
CPC分类号： C12N15/1006
摘要： The invention provides the use of tetraethylene glycol dimethyl ether for adsorbing nucleic acids to solid phases such as those with silica surfaces. To this end, the invention also provides compositions comprising TDE. Methods are disclosed and claimed to purify nucleic acids from samples, as well as kits useful for performing these methods. Particularly, the invention encompasses methods for the purification of nucleic acids with low molecular weight. The nucleic acids purified by a method of the invention are suited for assays aiming at the detection of a target nucleic acid.
摘要翻译： 本发明提供四乙二醇二甲醚用于将核酸吸附到固相上，例如具有二氧化硅表面的那些。 为此，本发明还提供了包含TDE的组合物。 方法被公开并要求从样品中纯化核酸，以及用于实施这些方法的试剂盒。 特别地，本发明包括用于纯化低分子量核酸的方法。 通过本发明的方法纯化的核酸适合用于检测靶核酸的测定。
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公开(公告)号：US20100086923A1
公开(公告)日：2010-04-08
申请号：US12480981
申请日：2009-06-09
CPC分类号： C12N15/1013 , C12N15/1003 , C12Q1/6806 , C12Q2527/125
摘要： The invention provides the use of water-miscible liquid cyclic acetals for adsorbing a nucleic acid to a solid phase such as a substrate with a silica surface. To this end, the invention also provides compositions comprising said acetals. Methods are disclosed and claimed to purify nucleic acids from samples, as well as kits useful for performing these methods. The nucleic acids purified by a method of the invention are suited for assays aiming at the detection of a target nucleic acid.
摘要翻译： 本发明提供了水混溶性液体环状缩醛用于将核酸吸附到固相（例如具有二氧化硅表面的基底）的用途。 为此，本发明还提供包含所述缩醛的组合物。 方法被公开并要求从样品中纯化核酸，以及用于实施这些方法的试剂盒。 通过本发明的方法纯化的核酸适合用于检测靶核酸的测定。
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