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    Enzymatic hydrolysis of biomasses having a high dry matter (DM) content
    21.
    发明授权
    Enzymatic hydrolysis of biomasses having a high dry matter (DM) content 有权
    具有高干物质（DM）含量的生物质的酶水解

    公开(公告)号：US07598069B2

    公开(公告)日：2009-10-06

    申请号：US12102976

    申请日：2008-04-15

    Applicant: Claus Felby ,  Jan Larsen ,  Henning Jørgensen ,  Jakob Vibe-Pedersen

    Inventor： Claus Felby ,  Jan Larsen ,  Henning Jørgensen ,  Jakob Vibe-Pedersen

    IPC: C12N9/42 ,  C12P7/10

    CPC classification number: C12P19/12 ,  C12P7/10 ,  C12P19/14 ,  Y02E50/16 ,  Y02E50/343

    Abstract: Disclosed are processes for liquefaction and saccharification of polysacharide containing biomasses having high dry matter content (>20%) and preferably possessing large average particle size. The polysaccharide containing biomasses are subject to enzymatic hydrolysis in a mixer that utilizes “free fall” mixing. “Free fall” mixing provides mechanical degradation of the biomass during hydrolysis and, in the case of lignocellulosic biomass, promotes movement of cellulase enzymes along cellulose chains, which improves enzymatic hydrolysis at high dry matter.

    Abstract translation: 公开了具有高干物质含量（> 20％）且优选具有大的平均粒度的含有多糖的生物质的液化和糖化的方法。 含有多糖的生物质在利用“自由落体”混合的混合器中进行酶水解。 “自由落体”混合在水解期间提供生物质的机械降解，并且在木质纤维素生物质的情况下促进纤维素酶沿着纤维素链的移动，这改善了在高干物质下的酶水解。

    METHODS OF PROCESSING LIGNOCELLULOSIC BIOMASS USING SINGLE-STAGE AUTOHYDROLYSIS PRETREATMENT AND ENZYMATIC HYDROLYSIS
    27.
    发明申请
    METHODS OF PROCESSING LIGNOCELLULOSIC BIOMASS USING SINGLE-STAGE AUTOHYDROLYSIS PRETREATMENT AND ENZYMATIC HYDROLYSIS 有权
    使用单级自动溶解预处理和酶水解处理线粒体生物量的方法

    公开(公告)号：US20160160253A1

    公开(公告)日：2016-06-09

    申请号：US14908913

    申请日：2014-02-05

    Applicant: INBICON A/S

    Inventor： Jan Larsen ,  Niels Nielsen Poulsen ,  Martin Dan Jeppesen ,  Kit Kellebjerg Mogensen

    IPC: C12P19/14 ,  C12P19/02

    CPC classification number: C12P19/14 ,  C12P5/023 ,  C12P7/10 ,  C12P19/02 ,  C12P2201/00 ,  D21C1/02 ,  D21C1/04 ,  D21C5/005 ,  Y02E50/16 ,  Y02E50/343

    Abstract: Methods of processing lignocellulosic biomass to fermentable sugars are provided which rely on hydrothermal pretreatment. Soft lignocellulosic biomass feedstock is pretreated in a single-stage pressurized hydrothermal pretreatment to very low severity. The pre-treated biomass is hydrolysed, typically as a whole slurry, using enzymatic hydrolysis catalysed by an enzyme mixture comprising endoglucanase, exoglucanase, β-glucosidase, endoxylanase, and β-xylosidase activities at activity levels in nkat/g glucan of endoglucanase of at least 1100, exoglucanase of at least 280, β-glucosidase of at least 3000, endoxylanase of at least 1400, and β-xylosidase of at least 75, so as to produce a hydrolysate in which the yield of C5 monomers is at least 55% of the original xylose and arabinose content of the feedstock prior to pretreatment.

    Abstract translation: 提供了依靠水热预处理将木质纤维素生物质加工成可发酵糖的方法。 软木质纤维素生物质原料在单级加压水热预处理中预处理至非常低的严重性。 使用由含有内切葡聚糖酶，外切葡聚糖酶，葡萄糖苷酶，内切木聚糖酶和β-木糖苷酶活性的酶混合物催化的酶水解，预处理的生物质通常作为整体浆液水解，其活性水平在内切葡聚糖酶的nkat / g葡聚糖 至少为1100，至少280的外切葡聚糖酶，至少为3000的葡萄糖苷酶，至少为1400的内切木聚糖酶，至少为75的木糖苷酶，以及至少75的β-木糖苷酶，以产生其中C5单体的产率为 在预处理之前，原料中原始木糖和阿拉伯糖含量的至少55％。

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