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137 条记录 (耗时 0.036 秒)

    Affinity capture of peptides by microarray and related methods
    21.
    发明授权
    Affinity capture of peptides by microarray and related methods 有权
    通过微阵列和相关方法亲和捕获肽

    公开(公告)号：US07794947B2

    公开(公告)日：2010-09-14

    申请号：US10888084

    申请日：2004-07-09

    申请人： Rudolf H. Aebersold ,  Hui Zhang

    发明人： Rudolf H. Aebersold ,  Hui Zhang

    IPC分类号： G01N33/53

    CPC分类号： G01N33/6848 ,  C12Q1/37 ,  G01N33/6845

    摘要： The invention provides methods of detecting polypeptides in a sample. The method can include the steps of cleaving polypeptides in a test sample to generate peptides; adding a predetermined amount of isotopically labeled peptide standards to the cleaved test sample, wherein the peptide standards correspond to peptides cleaved with the same reagent used to cleave the test sample; contacting the cleaved test sample containing peptide standards with an array of immobilized binding agents specific for the peptide standards; washing the array to remove unbound peptides, thereby retaining affinity captured sample peptides and standard peptides; analyzing the affinity captured peptides using mass spectrometry; and determining the presence of bound test peptides and standard peptides. The method can further include the step of quantifying the amount of the test peptides by comparing the ratio of test peptide to corresponding standard peptide.

    摘要翻译： 本发明提供了检测样品中多肽的方法。 该方法可以包括在测试样品中切割多肽以产生肽的步骤; 向切割的测试样品中加入预定量的同位素标记的肽标准物，其中肽标准物对应于用用于切割测试样品的相同试剂切割的肽; 使含有肽标准物的切割的测试样品与肽标准物特异性的固定化结合剂阵列接触; 洗涤阵列以除去未结合的肽，从而保留亲和力捕获的样品肽和标准肽; 使用质谱分析亲和力捕获的肽; 并确定结合的测试肽和标准肽的存在。 该方法还可以包括通过比较测试肽与相应的标准肽的比例来量化测试肽的量的步骤。

    KRAS-specific capture agents, compositions, and methods of making and using
    26.
    发明授权
    KRAS-specific capture agents, compositions, and methods of making and using 有权

    公开(公告)号：US11414460B2

    公开(公告)日：2022-08-16

    申请号：US16933679

    申请日：2020-07-20

    申请人： Institute for Systems Biology

    发明人： James R. Heath

    IPC分类号： A61K38/00 ,  C07K7/50

    摘要： Disclosed are compounds, compositions, and methods involving cyclic peptides that can bind to KRAS (G12D) oncogenic protein. For example, disclosed are cyclic peptides that selectively bind KRAS (G12D) oncogenic protein. Also disclosed are methods of inhibiting KRAS (G12D) oncogenic protein in a cancer cell expressing KRAS (G12D) oncogenic protein. In some forms, the method comprises incubating the cancer cell with any one or more of the disclosed cyclic peptides. In some forms, the method comprises bringing into contact the cancer cell with any one or more of the disclosed cyclic peptides.

    KRAS-Specific Capture Agents, Compositions, and Methods of Making and Using
    27.
    发明申请
    KRAS-Specific Capture Agents, Compositions, and Methods of Making and Using 有权

    公开(公告)号：US20210032293A1

    公开(公告)日：2021-02-04

    申请号：US16933679

    申请日：2020-07-20

    申请人： Institute for Systems Biology

    发明人： James R. Heath

    IPC分类号： C07K7/50

    摘要： Disclosed are compounds, compositions, and methods involving cyclic peptides that can bind to KRAS (G12D) oncogenic protein. For example, disclosed are cyclic peptides that selectively bind KRAS (G12D) oncogenic protein. Also disclosed are methods of inhibiting KRAS (G12D) oncogenic protein in a cancer cell expressing KRAS (G12D) oncogenic protein. In some forms, the method comprises incubating the cancer cell with any one or more of the disclosed cyclic peptides. In some forms, the method comprises bringing into contact the cancer cell with any one or more of the disclosed cyclic peptides.