公开(公告)号：US20170032079A1

公开(公告)日：2017-02-02

申请号：US15259311

申请日：2016-09-08

Applicant: Yeda Research and Development Co. Ltd.

Inventor： Sarel FLEISHMAN ,  Adi Goldenzweig ,  Dan S. Tawfik

IPC: G06F19/16 ,  C12N9/16 ,  G06F19/00 ,  C40B30/02 ,  G06F19/22 ,  C12N9/18 ,  C12N9/10

CPC classification number: G16B15/00 ,  C12N9/1007 ,  C12N9/16 ,  C12N9/18 ,  C12Y201/01037 ,  C12Y301/01007 ,  C12Y301/08001 ,  G16B15/20 ,  G16B30/00 ,  G16B35/00 ,  G16C20/10 ,  G16C20/60

Abstract: A method for designing and selecting a protein having a stabilized structure compared to a corresponding wild type protein, and proteins having at least six amino acid substitutions with respect to a corresponding wild type protein, designed for improved thermal stability, improved specific activity and/or improved expression levels, are provided herein.

Abstract translation: 与相应的野生型蛋白质相比，设计和选择具有稳定结构的蛋白质的方法，以及相对于相应野生型蛋白质具有至少6个氨基酸取代的蛋白质，其设计用于改善热稳定性，改善的比活性和/或 改善的表达水平。

公开(公告)号：US08962333B2

公开(公告)日：2015-02-24

申请号：US13439069

申请日：2012-04-04

Applicant: Janet Westpheling ,  DaeHwan Chung ,  Jennifer Huddleston ,  Joel A. Farkas

Inventor： Janet Westpheling ,  DaeHwan Chung ,  Jennifer Huddleston ,  Joel A. Farkas

IPC: C12N15/74 ,  C12N9/22 ,  C12N9/10 ,  C12P19/34

CPC classification number: C12N9/22 ,  C12N9/1007 ,  C12P19/34 ,  C12Y201/01037 ,  C12Y301/21004

Abstract: The present invention relates to the discovery of a novel restriction/modification system in Caldicellulosiruptor bescii. The discovered restriction enzyme is a HaeIII-like restriction enzyme that possesses a thermophilic activity profile. The restriction/modification system also includes a methyltransferase, M.CbeI, that methylates at least one cytosine residue in the CbeI recognition sequence to m4C. Thus, the invention provides, in various aspects, isolated CbeI or M.CbeI polypeptides, or biologically active fragments thereof; isolated polynucleotides that encode the CbeI or M.CbeI polypeptides or biologically active fragments thereof, including expression vectors that include such polynucleotide sequences; methods of digesting DNA using a CbeI polypeptide; methods of treating a DNA molecule using a M.CbeI polypeptide; and methods of transforming a Caldicellulosiruptor cell.

Abstract translation: 本发明涉及在Caldicellulosiruptor bescii中发现新的限制/修饰系统。 所发现的限制酶是具有嗜热活性分布的HaeIII样限制酶。 限制/修饰系统还包括甲基转移酶M.CbeI，其将CbeI识别序列中的至少一个胞嘧啶残基甲基化为m4C。 因此，本发明在各个方面提供了分离的CbeI或M.CbeI多肽或其生物活性片段; 编码CbeI或M.CbeI多肽或其生物活性片段的分离的多核苷酸，包括包括这种多核苷酸序列的表达载体; 使用CbeI多肽消化DNA的方法; 使用M.CbeI多肽处理DNA分子的方法; 和转化Caldicellulosiruptor细胞的方法。

公开(公告)号：US20240327812A1

公开(公告)日：2024-10-03

申请号：US18632018

申请日：2024-04-10

Applicant: Mammoth Biosciences, Inc.

Inventor： Lucas Benjamin HARRINGTON ,  Yuchen GAO ,  Yuxuan ZHENG

IPC: C12N9/22 ,  C12N9/10 ,  C12N9/78 ,  C12N15/11 ,  C12N15/86

CPC classification number: C12N9/22 ,  C12N9/1007 ,  C12N9/78 ,  C12N15/111 ,  C12N15/86 ,  C07K2319/80 ,  C12N2750/14143 ,  C12Y201/01037 ,  C12Y305/04002

Abstract: The present disclosure provides compositions of CRISPR associated (Cas) effector proteins fused to partner proteins. Compositions typically comprise a guide nucleic acid. Also disclosed are the methods and systems for detecting and modifying target nucleic acids using the same. The cells, progenies thereof, and populations thereof produced by the compositions, methods, or systems provided herein are also described.

公开(公告)号：US20170369855A1

公开(公告)日：2017-12-28

申请号：US15539256

申请日：2015-12-24

Applicant: DANA-FARBER CANCER INSTITUTE, INC. ,  The Johns Hopkins University

Inventor： Carl NOVINA ,  Glenna MEISTER ,  Marc OSTERMEIER ,  Tina XIONG

IPC: C12N9/10 ,  C12Q1/68 ,  C12N15/11 ,  C12N9/22 ,  C12N2310/20

CPC classification number: C12N9/1007 ,  A61K48/00 ,  C07K2319/09 ,  C07K2319/80 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/11 ,  C12N15/85 ,  C12N15/907 ,  C12N2310/20 ,  C12N2800/24 ,  C12N2800/40 ,  C12Q1/6897 ,  C12Q2600/154 ,  C12Y201/01 ,  C12Y201/01037 ,  C12Y301/00

Abstract: The present invention provides methods of systems and methods of site specific methylation.

公开(公告)号：US20170335402A1

公开(公告)日：2017-11-23

申请号：US15502623

申请日：2015-08-08

Applicant: Baylor College of Medicine

Inventor： Rachel Rau ,  Margaret A. Goodell

IPC: C12Q1/68 ,  A61K31/519 ,  A61K31/52

CPC classification number: C12Q1/6886 ,  A61K31/00 ,  A61K31/519 ,  A61K31/52 ,  C07H19/16 ,  C12Q2600/106 ,  C12Q2600/154 ,  C12Q2600/156 ,  C12Y201/01037

Abstract: The present disclosure relates to methods of treating AML, associated with DNMT3A mutations by administering one or more DOT1L inhibitors or related pharmaceutical compositions to subjects in need thereof.

公开(公告)号：US20170114318A1

公开(公告)日：2017-04-27

申请号：US15394468

申请日：2016-12-29

Applicant: SCARAB GENOMICS, LLC

Inventor： Frederick R. BLATTNER ,  Balint CSORGO ,  Gyorgy POSFAI

IPC: C12N1/08 ,  C12N9/10

CPC classification number: C12N1/08 ,  C12N9/1007 ,  C12N9/1252 ,  C12N15/70 ,  C12P21/00 ,  C12P21/02 ,  C12R1/19 ,  C12Y201/01037

Abstract: Reduced genome bacteria with improved genetic stability are provided. Also provided are methods of producing polypeptides using the reduced genome bacteria with improved genetic stability.

公开(公告)号：US09605268B2

公开(公告)日：2017-03-28

申请号：US14385940

申请日：2013-02-21

Applicant: Institute of Microbiology, Chinese Academy of Sciences

Inventor： Tingyi Wen ,  Guoqiang Zhang ,  Aihua Deng

IPC: C12N1/20 ,  C12N15/70 ,  C12R1/19 ,  C12N9/10 ,  C12N15/74 ,  C12N15/75

CPC classification number: C12N15/70 ,  C12N1/20 ,  C12N9/1007 ,  C12N15/74 ,  C12N15/75 ,  C12R1/19 ,  C12Y201/01037

Abstract: Disclosed is a method for introducing an exogenous DNA by overcoming the restriction modification barrier of the target bacterium. The method includes the steps of 1) co-expressing all DNA-methyltransferase-encoding genes in the genome of the target bacterium in E. coli in which the restriction modification system thereof has been deleted to obtain a recombinant bacterium A, 2) introducing an exogenous DNA molecule into the recombinant bacterium A for in vivo modification so as to obtain a methylation-modified exogenous DNA molecule, and 3) introducing the methylation-modified exogenous DNA molecule into the target bacterium.

公开(公告)号：US20170016017A1

公开(公告)日：2017-01-19

申请号：US14806867

申请日：2015-07-23

Applicant: Michael E Fromm

Inventor： Michael E Fromm

IPC: C12N15/82

CPC classification number: C12N15/8261 ,  C12N9/1007 ,  C12N9/22 ,  C12N15/8216 ,  C12Y201/01037 ,  Y02A40/146

Abstract: The present invention provides methods for obtaining plants that exhibit useful traits by expression of a DNA methyltransferase fusion protein in progenitor plants. Methods for identifying genetic loci that provide for useful traits in plants and plants produced with those loci are also provided. In addition, plants that exhibit the useful traits, parts of the plants including seeds, and products of the plants are provided as well as methods of using the plants. Recombinant DNA vectors and transgenic plants comprising those vectors that express a DNA methyltransferase fusion protein are also provided.

Abstract translation: 本发明提供了通过在祖细胞植物中表达DNA甲基转移酶融合蛋白获得表现出有用性状的植物的方法。 还提供了用于鉴定在这些基因座产生的植物和植物中提供有用性状的遗传基因座的方法。 此外，提供显示有用性状的植物，包括种子的植物的部分和植物的产物以及使用植物的方法。 还提供了重组DNA载体和包含表达DNA甲基转移酶融合蛋白的载体的转基因植物。

公开(公告)号：US20160032310A1

公开(公告)日：2016-02-04

申请号：US14716681

申请日：2015-05-19

Applicant: Michael E. Fromm

Inventor： Michael E. Fromm

IPC: C12N15/82 ,  A01H1/02 ,  C12N9/10

CPC classification number: C12N15/8261 ,  C12N9/1007 ,  C12Y201/01037 ,  Y02A40/146

Abstract: The present invention provides methods for obtaining plants that exhibit useful traits by expression of a recombinant DNA methyltransferase in progenitor plants. Methods for identifying genetic loci that provide for useful traits in plants and plants produced with those loci are also provided. In addition, plants that exhibit the useful traits, parts of the plants including seeds, and products of the plants are provided as well as methods of using the plants. Recombinant DNA vectors and transgenic plants comprising those vectors that express a recombinant DNA methyltransferase are also provided.

Abstract translation: 本发明提供了通过在祖细胞植物中表达重组DNA甲基转移酶来获得表现出有用性状的植物的方法。 还提供了用于鉴定在这些基因座产生的植物和植物中提供有用性状的遗传基因座的方法。 此外，提供显示有用性状的植物，包括种子的植物的部分和植物的产物以及使用植物的方法。 还提供了重组DNA载体和包含表达重组DNA甲基转移酶的那些载体的转基因植物。

公开(公告)号：US20150167004A1

公开(公告)日：2015-06-18

申请号：US14413004

申请日：2013-07-09

Applicant: METHEOR THERAPEUTICS CORPORATION

Inventor： Andrew Z. Sledziewski ,  Theodore Devos ,  Ryszard Kole

IPC: C12N15/113

CPC classification number: C12N15/1137 ,  C12N2310/13 ,  C12N2310/33 ,  C12N2310/3341 ,  C12N2310/531 ,  C12Y201/01037

Abstract: Modified oligonucleotides comprising CpG sites, wherein the cytosine is replaced by cytosine analogs are provided as well as methods of making the oligonucleotides and their use in inhibiting DNA Methyltransferase, inhibiting or reversing methylation of genes and in treating cancer, tumorigenesis and hyper-proliferative disorders.

Abstract translation: 提供了包含CpG位点的修饰的寡核苷酸，其中胞嘧啶被胞嘧啶类似物替代，以及制备寡核苷酸及其在抑制DNA甲基转移酶中的用途，抑制或逆转基因甲基化和治疗癌症，肿瘤发生和高增殖性疾病的方法。