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357 条记录 (耗时 0.031 秒)

    Controls and standards for assays and method for manufacture thereof
    24.
    发明申请
    Controls and standards for assays and method for manufacture thereof 失效
    测定的对照和标准及其制备方法

    公开(公告)号：US20040101972A1

    公开(公告)日：2004-05-27

    申请号：US10303947

    申请日：2002-11-25

    申请人： Agdia, Inc.

    发明人： Barbara A. Schoedel

    IPC分类号： C12Q001/70 ,  G01N033/558

    CPC分类号： G01N33/558 ,  G01N33/96 ,  Y10S435/967 ,  Y10T436/10 ,  Y10T436/105831 ,  Y10T436/108331

    摘要： Controls and standards for assays and method for manufacturing the controls and standards is described. The controls or standards comprise a porous carrier material which has quantitatively reversibly absorbed and dried thereon an analyte for the control or standard wherein the analyte is not degraded over time while it is absorbed to the porous carrier material. Preferably, the controls and standards are provided as a control strip (10) in which the porous carrier material (20) is adhered to the distal end (16) of a support member (12). To quantitatively elute the analyte from the carrier to a solvent for an assay, the carrier is contacted to the same solvent used for the samples in the assay. After the analyte has been eluted, the carrier is discarded and the assay is performed. The controls and standards are useful for assays which detect bacteria, fungi, viruses, and proteins.

    摘要翻译： 描述了用于测定的控制和标准以及用于制造控制和标准的方法。 对照物或标准品包括定量可逆地吸收并在其上干燥用于对照或标准物的分析物的多孔载体材料，其中分析物在被多孔载体材料吸收时不会随时间而降解。 优选地，控制和标准被提供为控制条（10），其中多孔载体材料（20）粘附到支撑构件（12）的远端（16）。 为了将分析物从载体定量洗脱至用于测定的溶剂，载体在测定中与用于样品的相同溶剂接触。 分析物被洗脱后，丢弃载体并进行测定。 对照和标准对于检测细菌，真菌，病毒和蛋白质的检测是有用的。

    Methods and reagents for cyanide-free determination of hemoglobin and leukocytes in whole blood
    25.
    发明授权
    Methods and reagents for cyanide-free determination of hemoglobin and leukocytes in whole blood 失效
    无血清测定全血中血红蛋白和白细胞的方法和试剂

    公开(公告)号：US06740527B1

    公开(公告)日：2004-05-25

    申请号：US08863121

    申请日：1997-05-27

    申请人： Show-Chu Wong ,  Sylvia Khoo

    发明人： Show-Chu Wong ,  Sylvia Khoo

    IPC分类号： G01N3348

    CPC分类号： G01N33/5094 ,  G01N33/721 ,  Y10S435/962 ,  Y10S435/967 ,  Y10S436/826 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/107497 ,  Y10T436/108331 ,  Y10T436/115831 ,  Y10T436/2525

    摘要： Cyanide-free reagents for the determination of hemoglobin and leukocytes present in a blood sample comprise an aqueous solutions of at least one quaternary ammonium salt, preferably selected from the group of alkyltrimethylammonium salts, alkyldimethylbenzylammonium salts or alkylpyridium salts consisting of tetradecyltrimethyl ammonium bromide (TTAB), dodecyltrimethyl ammonium chloride, cetyltrimethyl ammonium bromide, hexadecyltrimethyl ammonium bromide and benzalkonium chlorides, and hydroxylamine salts, especially hydrochloride, sulfate and phosphates and other acid salts. The method involves mixing the reagent with a diluent-diluted blood sample, presenting it to an absorbance spectrophotometer and measuring the resulting optical density as an indicator of hemoglobin concentration. This cyanide-free reagent could be used solely for hemoglobin determinations, or, it can also be used in leukocyte counting and sizing using hematology instrumentation.

    摘要翻译： 用于测定存在于血液样品中的血红蛋白和白细胞的无氰试剂包含至少一种季铵盐的水溶液，优选选自烷基三甲基铵盐，烷基二甲基苄基铵盐或由十四烷基三甲基溴化铵（TTAB）组成的烷基吡啶盐， 十六烷基三甲基氯化铵，十六烷基三甲基溴化铵，十六烷基三甲基溴化铵和苯扎氯铵，以及羟胺盐，特别是盐酸盐，硫酸盐和磷酸盐等酸盐。 该方法包括将试剂与稀释剂稀释的血液样品混合，将其呈现给吸光度分光光度计，并测量所得光密度作为血红蛋白浓度的指标。 该无氰试剂可用于血红蛋白测定，也可用于使用血液学仪器进行白细胞计数和上浆。

    Self-calibration system for a magnetic binding assay
    26.
    发明申请
    Self-calibration system for a magnetic binding assay 有权
    用于磁性结合测定的自校准系统

    公开(公告)号：US20040043512A1

    公开(公告)日：2004-03-04

    申请号：US10228837

    申请日：2002-08-27

    申请人： Kimberly-Clark Worldwide, Inc.

    发明人： Xuedong Song ,  Rosann Kaylor

    IPC分类号： G01N033/553

    CPC分类号： G01N33/582 ,  G01N33/54326 ,  Y10S435/967 ,  Y10S436/80 ,  Y10S436/905 ,  Y10T436/10 ,  Y10T436/101666 ,  Y10T436/105831

    摘要： A self-calibrated, magnetic binding assay (e.g., sandwich, competitive, etc.) for detecting the presence or quantity of an analyte residing in a test sample is provided. The magnetic binding assay includes detection probes capable of generating a detection signal (e.g., fluorescent non-magnetic particles) and calibration probes capable of generating calibration signal (e.g., fluorescent magnetic particles). The amount of the analyte within the test sample is proportional to the intensity of the detection signal calibrated by the intensity of the calibration signal.

    摘要翻译： 提供了用于检测驻留在测试样品中的分析物的存在或数量的自校准的磁性结合测定（例如，夹心，竞争性等）。 磁结合测定法包括能够产生检测信号的检测探针（例如，荧光非磁性颗粒）和能产生校准信号的校准探针（例如，荧光磁性颗粒）。 测试样品中分析物的量与由校准信号的强度校准的检测信号的强度成比例。

    Solid phase assay for detection of ligands
    27.
    发明授权
    Solid phase assay for detection of ligands 失效
    用于检测配体的固相测定

    公开(公告)号：US06664114B1

    公开(公告)日：2003-12-16

    申请号：US08277225

    申请日：1994-07-18

    申请人： Steve J. Lackie ,  Thomas R. Glass

    发明人： Steve J. Lackie ,  Thomas R. Glass

    IPC分类号： G01N33543

    CPC分类号： G01N21/6408 ,  G01N21/6428 ,  G01N21/75 ,  G01N33/54306 ,  G01N33/54313 ,  Y10S435/962 ,  Y10S435/967

    摘要： The present invention provides an improved system for detecting the presence or level of an analyte in a sample. In “competition-like” assays of the present invention, a sample including an analyte is mixed with a second ligand to which the analyte binds, and the mixture is exposed to a solid phase containing a first ligand that can compete with the analyte for binding to the second ligand. According to the present invention, the time of exposure of the mixture to the solid phase is limited so that substantially no dissociation of analyte/second ligand complex occurs. The competition-like assays of the present invention are preferably performed with a solid phase containing a substantial excess of first ligand. In “sandwich-type” assays of the present invention, a sample including an analyte is contacted with a solid phase including a first ligand that binds the analyte and, simultaneously or subsequently, is contacted with a second ligand that binds the analyte (or the analyte/first ligand complex). The time of contact between the second ligand and the solid phase is limited so that substantially no non-specific binding between the second ligand and the solid phase occurs.

    摘要翻译： 本发明提供了一种用于检测样品中分析物的存在或水平的改进​​系统。 在本发明的“竞争样”测定中，将包含分析物的样品与分析物结合的第二配体混合，并将混合物暴露于含有可与分析物竞争结合的第一配体的固相 到第二配体。 根据本发明，混合物暴露于固相的时间受到限制，从而基本上不发生分析物/第二配体络合物的解离。 本发明的竞争样试验优选用含有大量过量的第一配体的固相进行。 在本发明的“夹心型”测定中，将包含分析物的样品与包含结合分析物的第一配体的固相接触，并且同时或随后与结合分析物的第二配体（或 分析物/第一配体复合物）。 第二配体和固相之间的接触时间受到限制，使得第二配体和固相之间基本上不发生非特异性结合。

    Device and method for detecting polyvalent substances
    28.
    发明授权
    Device and method for detecting polyvalent substances 失效
    多价物质检测装置及方法

    公开(公告)号：US06653066B1

    公开(公告)日：2003-11-25

    申请号：US08261639

    申请日：1994-06-17

    申请人： Siegfried R. Krutzik

    发明人： Siegfried R. Krutzik

    IPC分类号： C12Q170

    CPC分类号： G01N33/558 ,  Y10S435/967 ,  Y10S435/971 ,  Y10S435/973 ,  Y10S435/974 ,  Y10S436/809 ,  Y10S436/81 ,  Y10S436/811 ,  Y10S436/825

    摘要： Disclosed is a device and method of use for detecting polyvalent analytes such as antibody to the AIDS virus, utilizing an inverse sandwich method. The test device comprises a first substance having an epitope, bound to a label and capable of moving within the test device. The test device further comprises a second substance immobilized to the test device and spatially separated from the first substance. The second substance has an epitope substantially similar to the epitope of the first substance. Upon application to the test device, the polyvalent analyte binds to the first substance and moves within the test device to the location of the second substance with both polyvalent analyte and first substance are immobilized at location of the second substance. Polyvalent analyte is detected by the presence of the label at the location of the second substance. Also disclosed is a control substance for use with the device that can be used to determine completion of the test and viability of the device.

    摘要翻译： 本发明公开了一种利用逆向夹心法检测多种分析物（如抗艾滋病病毒抗体）的装置和方法。 测试装置包括具有结合到标签上并能够在测试装置内移动的表位的第一物质。 测试装置还包括固定在测试装置上并与第一物质空间分离的第二物质。 第二物质具有与第一物质的表位基本上相似的表位。 当应用于测试装置时，多价分析物与第一物质结合并在测试装置内移动到第二物质的位置，同时多分析物和第一物质都被固定在第二物质的位置。 通过在第二物质的位置处存在标签来检测多价分析物。 还公开了一种用于该装置的控制物质，其可以用于确定装置的测试和活力的完成。

    Human procalcitonin and the preparation and use thereof
    30.
    发明授权
    Human procalcitonin and the preparation and use thereof 有权

    公开(公告)号：US06562946B2

    公开(公告)日：2003-05-13

    申请号：US09742373

    申请日：2000-12-22

    申请人： Harald Althaus ,  Hans-Peter Hauser

    发明人： Harald Althaus ,  Hans-Peter Hauser

    IPC分类号： C07K14585

    CPC分类号： C07K14/585 ,  A61K38/00 ,  C07K2319/00 ,  Y10S435/967

    摘要： The invention relates to human procalcitonin and the preparation and use thereof. In particular, a process for preparing human procalcitonin is described wherein a gene coding for a polypeptide comprising the amino acid sequence of human procalcitonin is inserted into a vector; a host organism is transformed with this gene-containing vector; and the polypeptide expressed by the host organism is isolated. Furthermore the use of the polypeptides according to the invention, in particular as medicaments and diagnostic agents is described.