公开(公告)号：US11932893B2

公开(公告)日：2024-03-19

申请号：US15971977

申请日：2018-05-04

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Robin E. Osterhout

IPC: C12P5/02 ,  C12N9/12 ,  C12N9/88 ,  C12N15/52 ,  C12P5/00

CPC classification number: C12P5/026 ,  C12N9/1205 ,  C12N9/1229 ,  C12N9/1235 ,  C12N9/88 ,  C12N15/52 ,  C12P5/002 ,  C12Y402/03 ,  Y02E50/30

Abstract: The invention provides non-naturally occurring microbial organisms containing an alkene pathway having at least one exogenous nucleic acid encoding an alkene pathway enzyme expressed in a sufficient amount to convert an alcohol to an alkene. The invention additionally provides methods of using such microbial organisms to produce an alkene, by culturing a non-naturally occurring microbial organism containing an alkene pathway as described herein under conditions and for a sufficient period of time to produce an alkene.

公开(公告)号：US11932845B2

公开(公告)日：2024-03-19

申请号：US14405390

申请日：2013-06-03

Applicant: Genomatica, Inc.

Inventor： Priti Pharkya ,  Anthony P. Burgard ,  Stephen J. Van Dien ,  Robin E. Osterhout ,  Mark J. Burk ,  John D. Trawick ,  Michael P. Kuchinskas ,  Brian Steer

IPC: C12N9/18 ,  C12P7/42 ,  C12P7/52 ,  C12N1/20 ,  C12P7/18 ,  C12N15/52 ,  C12N1/14 ,  C12N1/16 ,  C12N15/63 ,  C07C31/20 ,  C07C59/01 ,  C08G18/28 ,  C08G18/32 ,  C08G63/06 ,  C08G63/16 ,  C08G63/181 ,  C08G69/02

CPC classification number: C12N1/20 ,  C07C31/207 ,  C07C59/01 ,  C08G18/284 ,  C08G18/3206 ,  C08G63/06 ,  C08G63/16 ,  C08G63/181 ,  C08G69/02 ,  C12N1/14 ,  C12N1/16 ,  C12N9/18 ,  C12N15/52 ,  C12N15/63 ,  C12P7/18 ,  C12P7/42 ,  C12Y301/01 ,  Y02P20/52

Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate, 1,4-butanediol, or other product pathway and being capable of producing 4-hydroxybutyrate, 1,4-butanediol, or other product, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate, 1,4-butanediol, or other product or related products using the microbial organisms.

公开(公告)号：US11753663B2

公开(公告)日：2023-09-12

申请号：US14652727

申请日：2013-12-16

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P17/10 ,  C12P13/02 ,  C12P7/24 ,  C12P19/02 ,  C12P7/44 ,  C12P13/00 ,  C12N9/04 ,  C08G63/78 ,  C08G73/02 ,  C08G69/16 ,  C08G69/08 ,  C12N15/52 ,  A23L29/00

CPC classification number: C12P17/10 ,  A23L29/065 ,  C08G63/78 ,  C08G69/08 ,  C08G69/16 ,  C08G73/0213 ,  C12N9/0006 ,  C12N15/52 ,  C12P7/24 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12Y101/01244

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as adipate, 6-aminocaproate, hexamethylenediamine or caprolactam. Also provided herein are methods for using such an organism to produce adipate, 6-aminocaproate, hexamethylenediamine or caprolactam.

公开(公告)号：US11702682B2

公开(公告)日：2023-07-18

申请号：US16927396

申请日：2020-07-13

Applicant: Genomatica, Inc.

Inventor： Lauri H. Suominen ,  Connor J. Galleher ,  Michael Japs ,  Mark J. Burk ,  Cara Tracewell

IPC: C12P13/00 ,  C12N9/88 ,  C12N1/02 ,  C12N1/20 ,  C12N9/96 ,  C12N11/16 ,  C12N15/52

CPC classification number: C12P13/001 ,  C12N1/02 ,  C12N1/20 ,  C12N9/88 ,  C12N9/96 ,  C12N11/16 ,  C12N15/52 ,  C12Y402/01001

Abstract: Provided is a method of producing and isolating a diamine produced by microbial fermentation that minimizes undesirable salt formation to provide a lower cost process.

公开(公告)号：US20230139515A1

公开(公告)日：2023-05-04

申请号：US17812636

申请日：2022-07-14

Applicant: Genomatica, Inc.

Inventor： Kevin Hoff ,  Cara Ann Tracewell ,  Kui Chan ,  Michael Kuchinskas ,  Harish Nagarajan

IPC: C12N9/04 ,  C12N9/10 ,  C12P7/18 ,  C12P7/24 ,  C12P7/52 ,  C12P19/32

Abstract: The present disclosure provides thiolases and polypeptide variants of 3-hydroxybutyryl-CoA dehydrogenase, nucleic acids encoding the same, vectors comprising the nucleic acids, and cells comprising the polypeptide variants and/or thiolase, the nucleic acids, and/or the vectors. The present disclosure also provides methods of making and using the same, including methods for culturing cells, and for the production of various products, including 3-hydroxybutyryl-CoA (3-HB-CoA), 3-hydroxybutyraldehyde (3-HBal), 3-hydroxybutyrate (3-HB), 1,3-butanediol (1,3-BDO), and esters and amides thereof, and products made from any of these.

公开(公告)号：US20230126921A1

公开(公告)日：2023-04-27

申请号：US17843589

申请日：2022-06-17

Applicant: Genomatica, Inc.

Inventor： Priti Pharkya ,  Anthony P. Burgard ,  Eric Roland Nunez Van Name

IPC: C12N15/52 ,  C12N9/88 ,  C12P7/18 ,  C12P7/42 ,  C12N9/12 ,  C12P5/02 ,  C12P7/04 ,  C12P7/40

Abstract: The invention provides non-natural microbial organisms containing enzymatic pathways and/or metabolic modifications for enhancing carbon flux through acetyl-CoA, or oxaloacetate and acetyl-CoA. Embodiments of the invention include microbial organisms having a pathway to acetyl-CoA and oxaloacetate that includes phosphoketolase (a PK pathway). The organisms also have either (i) a genetic modification that enhances the activity of the non-phosphotransferase system (non-PTS) for sugar uptake, and/or (ii) a genetic modification(s) to the organism's electron transport chain (ETC) that enhances efficiency of ATP production, that enhances availability of reducing equivalents or both. The microbial organisms can optionally include (iii) a genetic modification that maintains, attenuates, or eliminates the activity of a phosphotransferase system (PTS) for sugar uptake. The enhanced carbon flux through acetyl-CoA and oxaloacetate can be used for production of a bioderived compound, and the microbial organisms can further include a pathway capable of producing the bioderived compound.

公开(公告)号：US11613767B2

公开(公告)日：2023-03-28

申请号：US16884976

申请日：2020-05-27

Applicant: Genomatica, Inc.

Inventor： Jun Sun ,  Anthony P. Burgard ,  Priti Pharkya

IPC: C12P7/04 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88 ,  C12P7/02 ,  C12N15/52

Abstract: The invention provides a non-naturally occurring microbial organism having a microbial organism having at least one exogenous gene insertion and/or one or more gene disruptions that confer production of primary alcohols. A method for producing long chain alcohols includes culturing these non-naturally occurring microbial organisms.

公开(公告)号：US20220347192A1

公开(公告)日：2022-11-03

申请号：US17640196

申请日：2020-09-04

Applicant: Genomatica, Inc.

Inventor： Michael A. Noble ,  Kevin G. Hoff ,  Russell S. Komor

IPC: A61K31/60 ,  C12N1/20 ,  C12N15/52 ,  C12P7/42 ,  C12P17/06 ,  A61K31/05 ,  A61K31/352 ,  A61K35/742 ,  A61K35/74 ,  A61K36/062 ,  A61K36/02

Abstract: Described herein are non-natural variants of prenyltronsfcrases having at least one amino acid substitution as compared to its corresponding natural or unmodified prenyltransferascs. The variants are capable of an increased rate of formation of prenylated aromatic compounds, such as cannabinoids, as compared to a wild type control The prcnyltransferase variants can be expressed in an engineered microbe having a pathway to such cannabinoids, and optionally can include one or more other pathway transgencs to promote formation of substrate(s) for the prcnyltransferases. Therapeutically useful cannabinoids can be purified from engineered cells and cell cultures.

公开(公告)号：US20220340913A1

公开(公告)日：2022-10-27

申请号：US17691010

申请日：2022-03-09

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Priti Pharkya ,  Robin E. Osterhout

IPC: C12N15/52 ,  C12P13/00 ,  C12P13/02 ,  C12P17/10 ,  C12P7/44 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12N9/80 ,  C12N9/88 ,  C12P7/46 ,  C12P7/62

Abstract: The invention provides a non-naturally occurring microbial organism having an adipate, 6-aminocaproic acid or caprolactam pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective adipate, 6-aminocaproic acid or caprolactam pathway. The invention additionally provides a method for producing adipate, 6-aminocaproic acid or caprolactam. The method can include culturing an adipate, 6-aminocaproic acid or caprolactam producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an adipate, 6-aminocaproic acid or caprolactam pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce adipate, 6-aminocaproic acid or caprolactam.

公开(公告)号：US20220333142A1

公开(公告)日：2022-10-20

申请号：US17605120

申请日：2020-04-24

Applicant: Genomatica, Inc.

Inventor： Amit M. Shah ,  Harish Nagarajan ,  Michael Angus Noble

IPC: C12P7/40 ,  C12P13/00 ,  C12N1/20 ,  C12N15/70 ,  C12N15/52

Abstract: Disclosed are trans-enoyl CoA reductase (TER) enzymes and nucleic acids encoding them. In some cases, the TER enzymes are non-natural, engineered trans-enoyl CoA reductase. TER enzymes were shown to catalyse the conversion of 5-carboxy-2-pentenoyl-CoA into adipyl-CoA for improved adipate production and the conversion of crotonyl-CoA into 6-aminocaproate. The enzymes can be used in biosynthetic methods and engineered microorganisms that enhance or improve the biosynthesis of 6-aminocaproate, hexamethylenediamine, caproic acid, caprolactone, or caprolactam. The engineered microorganisms include exogenous TER and in some cases engineered TER.