公开(公告)号：US11021695B2

公开(公告)日：2021-06-01

申请号：US15958229

申请日：2018-04-20

Applicant: GENOMATICA, INC.

Inventor： Louis Hom ,  Na Trinh ,  Murtaza Alibhai ,  Zhihao Hu ,  Eli Groban ,  Vikranth Arlagadda ,  Elizabeth Clarke

IPC: C12N9/16 ,  C12N15/00 ,  C12N9/18 ,  C12N15/63 ,  C10L1/02 ,  C07H21/04 ,  C12P21/06

Abstract: The present invention relates to novel mutant thioesterase enzymes and naturally-occurring equivalents thereof, compositions made from such enzymes and uses of thioesterase enzymes. In particular, the present invention provides mutant thioesterase enzymes that have altered properties, for example, altered substrate specificity, altered activity, altered selectivity, and/or altered proportional yields in the product mixtures. The present invention also provides polynucleotides encoding such mutant thioesterase enzymes, and vectors and host cells comprising such polynucleotides. The invention further provides for novel uses of thioesterases in the production of various fatty acid derivatives, which are useful as, or as components of, industrial chemicals and fuels.

公开(公告)号：US20210147883A1

公开(公告)日：2021-05-20

申请号：US16785510

申请日：2020-02-07

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Priti Pharkya ,  Tae Hoon Yang ,  Jungik Choi

IPC: C12P7/18 ,  C12N9/02 ,  C12N9/12 ,  C12N9/16 ,  C12N15/52 ,  C12N1/20 ,  C12N9/00 ,  C07K14/245 ,  C12P7/42 ,  C12N9/52 ,  C12N9/88

Abstract: The invention is directed to a non-naturally occurring microbial organism comprising a first attenuation of a succinyl-CoA synthetase or transferase and at least a second attenuation of a succinyl-CoA converting enzyme or a gene encoding a succinate producing enzyme within a multi-step pathway having a net conversion of succinyl-CoA to succinate.

公开(公告)号：US20210108232A1

公开(公告)日：2021-04-15

申请号：US16884976

申请日：2020-05-27

Applicant: Genomatica, Inc.

Inventor： Jun Sun ,  Anthony P. Burgard ,  Priti Pharkya

IPC: C12P7/04 ,  C12N9/04 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88 ,  C12P7/02 ,  C12N15/52

Abstract: The invention provides a non-naturally occurring microbial organism having a microbial organism having at least one exogenous gene insertion and/or one or more gene disruptions that confer production of primary alcohols. A method for producing long chain alcohols includes culturing these non-naturally occurring microbial organisms.

公开(公告)号：US20210071212A1

公开(公告)日：2021-03-11

申请号：US16915454

申请日：2020-06-29

Applicant: Genomatica, Inc.

Inventor： Andreas W. Schirmer ,  Haibo Wang ,  Stephen B. Del Cardayre ,  Zhihao Hu ,  Louis G. Hom ,  Baolong Zhu ,  Cindy Chang ,  Emanuela E. Popova

IPC: C12P7/64 ,  C12N9/02 ,  C12P7/42 ,  C12N15/62 ,  C12P7/18 ,  C12N15/52 ,  C12N9/04

Abstract: The disclosure relates to omega-hydroxylated fatty acid derivatives and methods of producing them. Herein, the disclosure encompasses a novel and environmentally friendly production method that provides omega-hydroxylated fatty acid derivatives at high purity and yield. Further encompassed are recombinant microorganisms that produce omega-hydroxylated fatty acid derivatives through selective fermentation.

公开(公告)号：US20210040511A1

公开(公告)日：2021-02-11

申请号：US16997704

申请日：2020-08-19

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Mark J. Burk ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C12P7/42 ,  C12P7/40 ,  C12N9/02 ,  C12N9/10 ,  C12N9/88 ,  C12N9/90

Abstract: The invention provides a non-naturally occurring microbial organism having a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway. The invention additionally provides a method for producing 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid. The method can include culturing a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid producing microbial organism expressing at least one exogenous nucleic acid encoding a 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid pathway enzyme in a sufficient amount and culturing under conditions and for a sufficient period of time to produce 2-hydroxyisobutyric acid, 3-hydroxyisobutyric acid or methacrylic acid.

公开(公告)号：US10711289B2

公开(公告)日：2020-07-14

申请号：US15538448

申请日：2015-12-22

Applicant: Genomatica, Inc.

Inventor： Lauri H. Suominen ,  Connor J. Galleher ,  Michael Japs ,  Mark J. Burk ,  Cara Tracewell

IPC: C12P13/00 ,  C12N11/16 ,  C12N9/96 ,  C12N9/88 ,  C12N1/20 ,  C12N15/52 ,  C12N1/02

Abstract: Provided is a method of producing and isolating a diamine produced by microbial fermentation that minimizes undesirable salt formation to provide a lower cost process.

公开(公告)号：US10711288B2

公开(公告)日：2020-07-14

申请号：US14897285

申请日：2014-06-16

Applicant: GENOMATICA, INC.

Inventor： Andreas W. Schirmer ,  Haibo Wang ,  Stephen B. Del Cardayre ,  Zhihao Hu ,  Louis G. Hom ,  Baolong Zhu ,  Cindy Chang ,  Emanuela E. Popova

IPC: C12P7/64 ,  C12N1/21 ,  C12N9/04 ,  C12N9/02 ,  C12N9/88 ,  C12N9/10 ,  C12N9/06 ,  C12N15/53 ,  C12N15/54 ,  C12N9/60 ,  C07K19/00 ,  C12P7/42 ,  C12N15/62 ,  C12P7/18 ,  C12N15/52

Abstract: The disclosure relates to omega-hydroxylated fatty acid derivatives and methods of producing them. Herein, the disclosure encompasses a novel and environmentally friendly production method that provides omega-hydroxylated fatty acid derivatives at high purity and yield. Further encompassed are recombinant microorganisms that produce omega-hydroxylated fatty acid derivatives through selective fermentation.

公开(公告)号：US10662451B2

公开(公告)日：2020-05-26

申请号：US15971878

申请日：2018-05-04

Applicant: Genomatica, Inc.

Inventor： Jun Sun ,  Mark J. Burk ,  Anthony P. Burgard ,  Robin E. Osterhout ,  Wei Niu ,  John D. Trawick ,  Robert Haselbeck

IPC: C12N1/20 ,  C12N1/14 ,  C12P17/04 ,  C12P7/18 ,  C12N15/52

Abstract: The invention provides non-naturally occurring microbial organisms comprising 1,4-butanediol (14-BDO) and gamma-butyrolactone (GBL) pathways comprising at least one exogenous nucleic acid encoding a 14-BDO and GBL pathway enzyme expressed in a sufficient amount to produce 14-BDO and GBL. The invention additionally provides methods of using such microbial organisms to produce 14-BDO and GBL.

公开(公告)号：US10662136B2

公开(公告)日：2020-05-26

申请号：US15975630

申请日：2018-05-09

Applicant: Genomatica, Inc.

Inventor： Warren Clark ,  Michael Japs ,  Mark J. Burk

IPC: C07C29/76 ,  C12P7/18

Abstract: A process of isolating 1,4-butanediol (1,4-BDO) from a fermentation broth includes separating a liquid fraction enriched in 1,4-BDO from a solid fraction comprising cells, removing water from said liquid fraction, removing salts from said liquid fraction, and purifying 1,4-BDO. A process for producing 1,4-BDO includes culturing a 1,4-BDO-producing microorganism in a fermentor for a sufficient period of time to produce 1,4-BDO. The 1,4-BDO-producing microorganism includes a microorganism having a 1,4-BDO pathway having one or more exogenous genes encoding a 1,4-BDO pathway enzyme and/or one or more gene disruptions. The process for producing 1,4-BDO further includes isolating 1,4-BDO.

公开(公告)号：US10563231B2

公开(公告)日：2020-02-18

申请号：US16209711

申请日：2018-12-04

Applicant: REG LIFE SCIENCES, LLC

Inventor： Andreas W. Schirmer ,  Mathew A. Rude ,  Shane A. Brubaker

IPC: C12N1/20 ,  C12P7/64 ,  C12N9/02 ,  C10L1/02 ,  C12N9/88 ,  C12P7/04 ,  C12P5/02 ,  C12P7/24

Abstract: Compositions and methods for producing aldehydes, alkanes, and alkenes are described herein. The aldehydes, alkanes, and alkenes can be used in biofuels.