Abstract:
The present invention relates to improved methods for processing fluids and to a fluid processing device (1) for use in a centrifuge comprising: (a) a first holder (14) form-fit to the shape of a first tube (18) for holding said first tube (18) whereby said first tube (18) has a first cross section (A1); and (b) a second holder (22) form-fit to the shape of a second tube (26) for holding said second tube (26) whereby said second tube (26) has a second cross section (A2) that is different from said first cross section (A1). With the fluid processing devices and the methods according to the invention, it is possible to simplify the centrifugal processing steps for a given fluid processing sequence and to automate them.
Abstract:
A rule set for an AVC system permits AVC operations to be performed at various levels of hierarchy within a governing budget data structure. A rule set contains a plurality of rules, each having an address field which relates an arbitrarily assigned control object to budget nodes in a budget data structure. Control objects typically are assigned to various units and aggregation levels within an organization and also across other dimensions. Rule arrays with several independent rule sets can be activated in parallel for checking an individual input data record against multiple budgetary requirements defined for the organization. This structure provides a comprehensive AVC control feature even for very large budget data structures and complex budgetary control environments.
Abstract:
A coach body for a multiple-unit railway vehicle is provided. The coach body includes two bogies at each end. The coach body has a length of at least 27.5 m and side walls of the coach body are designed such that, a maximum outside width in the longitudinal center of the coach being 2.86 m at the height of seat rests, an inside width of at least 2.6 m is achieved for the greatest part of the coach length.
Abstract:
The invention relates to a roller bearing having an inner bearing ring having a raceway and an outer bearing ring having a raceway and a plurality of rolling elements disposed between the raceways that are lubricated by a lubricant. The invention is characterized in that the surface of the inner bearing ring and/or of the outer bearing ring facing the rolling elements and/or the surface of a cage holding the rolling elements have a surface structure.
Abstract:
The invention relates to a method for decomposing a biological sample (9), said method having the following steps: the sample (9) is put into a container (6) which is composed of plastic, in particular, the container (6) is inserted into an adapter (2, 2a), the adapter with the closed container therein is connected to an apparatus which moves the adapter back and forth, in particular upwards, in automated fashion. This method makes it possible to decompose biological samples in automated fashion, to be precise both samples at room temperature and frozen samples. The invention also relates to an apparatus for carrying out the method, said apparatus comprising an adapter (2, 2a) which is predominantly composed of plastic and has sleeves (4) which are composed of metal and are intended to accommodate containers (6) which are composed of plastic, in particular.
Abstract:
A budgetary control system limits revenue generating transactions that may be entered in an enterprise management system. When a new revenue posting is proposed to the system, various AVC rules are evaluated. The evaluation compares the revenue posting against predetermined budgetary limits. The AVC rules may address various nodes within a revenue postings data structure and within a revenue budget data structure and they may define a test relationship that must be maintained between them. If proposed revenue posting would violate the test relationship of an AVC rule, then the revenue posting may be rejected from the system depending upon the contents of a response field in the AVC rule. Some AVC rules mandate that a violation cause the proposed transaction to be blocked while others may generate a warning notification within the system but admit the new transaction despite the violation.
Abstract:
A rail vehicle has a permanent-magnet synchronous motor as its traction motor. A switching apparatus is arranged between a converter and the traction motor, connects the traction motor to the converter in the driving mode and connects the traction motor to at least one braking resistance during generator operation of the traction motor. The switching apparatus can be operated pneumatically and is for this purpose connected to a main air control line of a compressed-air braking system. In the driving mode, when the pressure in the main air control line is greater than a threshold value, the traction motor is connected to the converter. For braking, when the pressure in the main air control line is less than the threshold value, the traction motor is connected to the at least one braking resistance.
Abstract:
The present invention relates to improved methods for processing fluids and to a fluid processing device (1) for use in a centrifuge comprising: (a) a first holder (14) form-fit to the shape of a first tube (18) for holding said first tube (18) whereby said first tube (18) has a first cross section (A1); and (b) a second holder (22) form-fit to the shape of a second tube (26) for holding said second tube (26) whereby said second tube (26) has a second cross section (A2) that is different from said first cross section (A1). With the fluid processing devices and the methods according to the invention, it is possible to simplify the centrifugal processing steps for a given fluid processing sequence and to automate them.
Abstract:
A method locating insertion elements (IS elements) or transposons in coryneform bacteria, a positive selection system suitable for the above, the IS elements found in this manner and their use, is disclosed. The method involves:(1) The construction of a non-self-transferrable vector mobilizable from an E. coli mobilizer strain which vector is composed of(a) A DNA segment containing a replicon functional in E. coli,(b) A second DNA segment containing the DNA fragment coding for the mobilization function (Mob site containing the oriT),(c) A third DNA segment which recombines homologously in Gram-positive bacteria and/or contains a replicon functional in coryneform bacteria,(d) A DNA segment from Bacillus subtilis containing the sacB gens,(2) Transfer of this vector by means of conjugative transfer into the coryneform recipient strains,(3) Cultivation of the transconjugants containing the vector in an .about.10% sucrose-containing nutrient medium,(4) Lysis of the sucrose-resistant clones, cleaving of the plasmids with restriction endonucleases and analysis of the fragments.