公开(公告)号：US20230069046A1

公开(公告)日：2023-03-02

申请号：US17971174

申请日：2022-10-21

Applicant: 10x Genomics, Inc.

Inventor： Jennifer Chew ,  Yifeng Yin ,  Layla Katiraee

IPC: C12Q1/6841

Abstract: Provided herein are methods for capturing an analyte from a first region of interest of a biological sample on a substrate, where the biological sample comprises the first region of interest and a second region, and where the method includes contacting the second region with a sealant in order to create a hydrophobic seal thereby preventing an interaction between an analyte from the second region with a capture domain of a capture probe.

公开(公告)号：US20230064372A1

公开(公告)日：2023-03-02

申请号：US17896520

申请日：2022-08-26

Applicant: 10x Genomics, Inc.

Inventor： James Michael Chell ,  Marlon Stoeckius ,  Jonathan Alles ,  Caroline Julie Gallant ,  Christina Galonska ,  Layla Katiraee

IPC: C12Q1/6876 ,  C12Q1/6811 ,  C12Q1/25 ,  C12Q1/6816 ,  C12Q1/6841 ,  C12Q1/6851

Abstract: Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample using RNA-templated ligation.

公开(公告)号：US20220356464A1

公开(公告)日：2022-11-10

申请号：US17871525

申请日：2022-07-22

Applicant: 10x Genomics, Inc.

Inventor： Hanyoup Kim ,  David Sukovich ,  Layla Katiraee ,  Augusto Manuel Tentori

IPC: C12N15/10

Abstract: Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.

公开(公告)号：US20220127666A1

公开(公告)日：2022-04-28

申请号：US17573119

申请日：2022-01-11

Applicant: 10x Genomics, Inc.

Inventor： Layla Katiraee ,  Michael Schnall-Levin ,  Christina Galonska ,  Marlon Stoeckius

IPC: C12Q1/6837 ,  C12Q1/6876 ,  C12Q1/6806

Abstract: Provided herein are methods of identifying a methylation status of an analyte in a biological sample. Also provided herein are methods that combine identifying the methylation status with spatial technology to identify the location of a methylation status in a biological sample.

公开(公告)号：US12287264B2

公开(公告)日：2025-04-29

申请号：US18784392

申请日：2024-07-25

Applicant: 10x Genomics, Inc.

Inventor： David Maurice Cox ,  Layla Katiraee

IPC: G01N1/36 ,  B01L9/00

Abstract: A substrate holder includes a base configured to receive a substrate; a cover configured to mateably engage with the base, the cover defining an opening formed by inner sidewalls; and a removable insert defining a surface, the removable insert being configured to be received within the opening of the cover. The removable insert includes a gasket; a projection coupled to the gasket; and at least two insert tabs extending from opposite sides of the removable insert, each insert tab being configured to engage with at least one of the inner sidewalls forming the opening of the cover.

公开(公告)号：US12098985B2

公开(公告)日：2024-09-24

申请号：US18357610

申请日：2023-07-24

Applicant: 10x Genomics, Inc.

Inventor： David Maurice Cox ,  Layla Katiraee

IPC: G01N1/36 ,  B01L9/00

CPC classification number: G01N1/36 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/0689 ,  B01L2300/043 ,  B01L2300/045 ,  B01L2300/0819 ,  G01N2001/368

Abstract: A substrate holder includes a base configured to receive a substrate; a cover configured to mateably engage with the base, the cover defining an opening formed by inner sidewalls; and a removable insert defining a surface, the removable insert being configured to be received within the opening of the cover. The removable insert includes a gasket; a projection coupled to the gasket; and at least two insert tabs extending from opposite sides of the removable insert, each insert tab being configured to engage with at least one of the inner sidewalls forming the opening of the cover.

公开(公告)号：US12060604B2

公开(公告)日：2024-08-13

申请号：US18302421

申请日：2023-04-18

Applicant: 10x Genomics, Inc.

Inventor： Layla Katiraee ,  Michael Schnall-Levin ,  Christina Galonska ,  Marlon Stoeckius

IPC: C12Q1/6837 ,  C12Q1/6806 ,  C12Q1/6876

CPC classification number: C12Q1/6837 ,  C12Q1/6806 ,  C12Q1/6876 ,  C12Q2600/154

Abstract: Provided herein are methods of identifying a methylation status of an analyte in a biological sample. Also provided herein are methods that combine identifying the methylation status with spatial technology to identify the location of a methylation status in a biological sample.

公开(公告)号：US20240141327A1

公开(公告)日：2024-05-02

申请号：US18409174

申请日：2024-01-10

Applicant: 10x Genomics, Inc.

Inventor： Hanyoup Kim ,  David Sukovich ,  Layla Katiraee ,  Augusto Manuel Tentori ,  Lauren Gutgesell ,  Janine Hensel ,  Seayar Mohabbat

IPC: C12N15/10

CPC classification number: C12N15/1065

Abstract: Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.

公开(公告)号：US20240110228A1

公开(公告)日：2024-04-04

申请号：US18503872

申请日：2023-11-07

Applicant: 10x Genomics, Inc.

Inventor： Cedric Uytingco ,  Layla Katiraee ,  Kristen Pham

IPC: C12Q1/6818 ,  C12N5/00 ,  C12Q1/6804 ,  C12Q1/6837 ,  C12Q1/6841 ,  C12Q1/6876 ,  G01N33/50 ,  G01N33/543

CPC classification number: C12Q1/6818 ,  C12N5/0062 ,  C12Q1/6804 ,  C12Q1/6837 ,  C12Q1/6841 ,  C12Q1/6876 ,  G01N33/5023 ,  G01N33/5088 ,  G01N33/54306 ,  C12Q2600/158 ,  G01N2458/10

Abstract: Provided herein are methods for simultaneous spatio-temporal measurement of gene expression and cellular activity.

公开(公告)号：US11873482B2

公开(公告)日：2024-01-16

申请号：US18140312

申请日：2023-04-27

Applicant: 10x Genomics, Inc.

Inventor： Hanyoup Kim ,  David Sukovich ,  Layla Katiraee ,  Augusto Manuel Tentori ,  Lauren Gutgesell ,  Janine Hensel ,  Seayar Mohabbat

IPC: C12N15/10

CPC classification number: C12N15/1065

Abstract: Provided herein are methods for capturing a connected probe and/or a capture handle sequence to a capture domain of a capture probe.