公开(公告)号：US12129474B2

公开(公告)日：2024-10-29

申请号：US18182701

申请日：2023-03-13

Applicant: Altria Client Services LLC

Inventor： Chengalrayan Kudithipudi ,  Dongmei Xu ,  James Strickland

IPC: C12N15/82 ,  A01H4/00 ,  A24B13/00 ,  A24B15/10 ,  A24B15/24 ,  B01F33/45 ,  B01F33/452 ,  B01F33/501 ,  B01F35/75 ,  B01L3/00 ,  C07K14/415 ,  C12N9/04 ,  G01N1/38 ,  H02N11/00 ,  G01N35/00 ,  G01N35/10

CPC classification number: C12N15/8243 ,  A01H4/005 ,  A01H4/008 ,  A24B13/00 ,  A24B15/10 ,  A24B15/245 ,  B01F33/45 ,  B01F33/452 ,  B01F33/50112 ,  B01F35/754251 ,  B01L3/502 ,  B01L3/50825 ,  C07K14/415 ,  C12N9/0006 ,  C12N15/8205 ,  C12N15/8225 ,  C12N15/825 ,  G01N1/38 ,  H02N11/006 ,  B01L2200/0684 ,  B01L2300/043 ,  B01L2300/0672 ,  B01L2300/0867 ,  B01L2400/0403 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0683 ,  G01N2035/00534 ,  G01N35/1079

Abstract: The present disclosure provides approaches for reducing tobacco-specific nitrosamines (TSNAs) in tobacco. Some of these approaches include genetically engineering tobacco plants to increase one or more antioxidants, increase oxygen radicle absorbance capacity (ORAC), or reduce nitrite. Also provided are methods and compositions for producing modified tobacco plants and tobacco products therefrom comprising reduced TSNAs.

公开(公告)号：US11859192B2

公开(公告)日：2024-01-02

申请号：US17571138

申请日：2022-01-07

Applicant: Altria Client Services LLC

Inventor： Marcos Fernando de Godoy Lusso ,  James A. Strickland ,  Jesse Frederick ,  Dongmei Xu ,  Chengalrayan Kudithipudi

IPC: C12N15/82 ,  A24B13/00

CPC classification number: C12N15/8225 ,  A24B13/00 ,  C12N15/8218 ,  C12N15/8243 ,  C12N15/8261

Abstract: The present disclosure includes methods and compositions for improving leaf quality in low-alkaloid tobacco plants, e.g., by combining inducible promoters and non-coding RNAs for suppression of an ornithine decarboxylase (ODC) gene. Also provided are low alkaloid tobacco plants with normal, suppressed, or otherwise altered polyamine levels. Further provided are tobacco plants with altered total alkaloid, nicotine levels, commercially acceptable leaf grade, their development via breeding or transgenic approaches, and production of tobacco products from these tobacco plants.

公开(公告)号：US11634724B2

公开(公告)日：2023-04-25

申请号：US16838762

申请日：2020-04-02

Applicant: Altria Client Services LLC

Inventor： Chengalrayan Kudithipudi ,  Dongmei Xu ,  James Strickland

IPC: C12N15/82 ,  A24B15/24 ,  A24B13/00 ,  A24B15/10 ,  C12N9/04 ,  B01L3/00 ,  G01N1/38 ,  H02N11/00 ,  B01F33/45 ,  B01F33/501 ,  B01F35/75 ,  A01H4/00 ,  C07K14/415 ,  G01N35/10 ,  G01N35/00

Abstract: The present disclosure provides approaches for reducing tobacco-specific nitrosamines (TSNAs) in tobacco. Some of these approaches include genetically engineering tobacco plants to increase one or more antioxidants, increase oxygen radicle absorbance capacity (ORAC), or reduce nitrite. Also provided are methods and compositions for producing modified tobacco plants and tobacco products therefrom comprising reduced TSNAs.

公开(公告)号：US11266174B2

公开(公告)日：2022-03-08

申请号：US15727418

申请日：2017-10-06

Applicant: Altria Client Services LLC

Inventor： Jesse Frederick ,  Chengalrayan Kudithipudi ,  Dongmei Xu

IPC: C12N15/82 ,  A24B3/12 ,  A24B15/20 ,  C12N9/88 ,  C12N15/10 ,  C12N15/66 ,  A61K38/00

Abstract: This disclosure provides a number of sequences involved in nitrogen utilization, methods of using such sequences, tobacco plants carrying modifications to such sequences, tobacco plants transgenic for such sequences, and tobacco products made from such plants.

公开(公告)号：US10851384B2

公开(公告)日：2020-12-01

申请号：US16149456

申请日：2018-10-02

Applicant: Altria Client Services LLC

Inventor： Chengalrayan Kudithipudi ,  Alec J. Hayes

IPC: C12N15/82 ,  A01H1/06 ,  A24B13/00 ,  A01H5/12

Abstract: This disclosure provides tobacco plants having a mutation in PR50 and transgenic tobacco plants containing a PR50 RNAi, and methods of making and using such plants.

公开(公告)号：US10647989B2

公开(公告)日：2020-05-12

申请号：US15727523

申请日：2017-10-06

Applicant: Altria Client Services LLC

Inventor： Chengalrayan Kudithipudi ,  Dongmei Xu ,  James Strickland

IPC: C12N15/82 ,  A24B15/24 ,  A24B15/10 ,  C12N9/04 ,  A01H4/00 ,  A24B13/00 ,  C07K14/415

Abstract: The present disclosure provides approaches for reducing tobacco-specific nitrosamines (TSNAs) in tobacco. Some of these approaches include genetically engineering tobacco plants to increase one or more antioxidants, increase oxygen radical absorbance capacity (ORAC), or reduce nitrite. Also provided are methods and compositions for producing modified tobacco plants and tobacco products therefrom comprising reduced TSNAs.

公开(公告)号：US20190241900A1

公开(公告)日：2019-08-08

申请号：US16228035

申请日：2018-12-20

Applicant: Altria Client Services LLC

Inventor： Chengalrayan Kudithipudi ,  Raja Payyavula ,  Dong Qi ,  Yanxin Shen

IPC: C12N15/82

CPC classification number: C12N15/825 ,  C12N15/8262

Abstract: The present specification provides for, and includes recombinant polynucleotides that encode plant-native, callus-preferred promoters operably linked to a coding region for a visual marker gene. The present specification also provides for, and includes plants comprising the recombinant nucleotides, methods to select callus comprising the disclosed recombinant nucleotides, and methods to create plants comprising the recombinant nucleotides.

公开(公告)号：US20190069506A1

公开(公告)日：2019-03-07

申请号：US16119366

申请日：2018-08-31

Applicant: Altria Client Services LLC

Inventor： Jesse Frederick ,  Chengalrayan Kudithipudi ,  Dongmei Xu

IPC: A01H6/82 ,  A01H5/10 ,  C12N15/82

Abstract: The present disclosure provides metabolic signatures and genetic markers for tracking enhanced nitrogen utilization efficiency phenotypes in tobacco plants and for introgressing enhanced nitrogen utilization efficiency phenotypes into tobacco plants. The disclosure also provides tobacco plants comprising enhanced nitrogen utilization efficiency and methods to the creation of tobacco plants comprising enhanced nitrogen utilization efficiency. The disclosure also provides recombinant polynucleotides and polypeptides for enhancing nitrogen utilization efficiency in modified tobacco plants and tobacco plants comprising the provided recombinant polynucleotides and polypeptides.

公开(公告)号：US20190055574A1

公开(公告)日：2019-02-21

申请号：US16149456

申请日：2018-10-02

Applicant: Altria Client Services LLC

Inventor： Chengalrayan Kudithipudi ,  Alec J. Hayes

IPC: C12N15/82 ,  A01H1/06 ,  A24B13/00 ,  A01H5/12

Abstract: This disclosure provides tobacco plants having a mutation in PR50 and transgenic tobacco plants containing a PR50 RNAi, and methods of making and using such plants.

公开(公告)号：US09781929B2

公开(公告)日：2017-10-10

申请号：US15009926

申请日：2016-01-29

Applicant: Altria Client Services LLC ,  Micreos BV

Inventor： Elisabeth Miller ,  Ujwala Warek ,  Dongmei Xu ,  Fritz Eichenseher ,  Steven Hagens ,  Chengalrayan Kudithipudi

IPC: A24B15/20 ,  C07K14/005 ,  C12N7/00 ,  A01N37/18 ,  A24B15/18 ,  A24B15/24 ,  A24B15/30

CPC classification number: A01N37/18 ,  A24B15/183 ,  A24B15/20 ,  A24B15/245 ,  A24B15/30 ,  C07K14/005 ,  C12N7/00 ,  C12N2795/00021 ,  C12N2795/00022 ,  C12N2795/00031

Abstract: Bacteriophage are provided, and methods of making and using the bacteriophage also are provided.