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    Recombinant poxvirus host range selection system
    37.
    发明授权
    Recombinant poxvirus host range selection system 失效
    重组痘病毒宿主范围选择系统

    公开(公告)号：US5225336A

    公开(公告)日：1993-07-06

    申请号：US902428

    申请日：1992-06-23

    申请人： Enzo Paoletti

    发明人： Enzo Paoletti

    IPC分类号： A61K39/00 ,  C07K14/03 ,  C07K14/145 ,  C12N7/01 ,  C12N15/863

    CPC分类号： C12N15/86 ,  C07K14/005 ,  A61K39/00 ,  C12N2710/16722 ,  C12N2710/24143 ,  C12N2760/20122

    摘要： What is described is a modified recombinant virus for expressing a gene product in a host. The modified recombinant virus has host range genes deleted therefrom so that the virus has restricted replication in the host. The modified recombinant virus also contains DNA which codes for and expresses the gene product in the host even with restricted replication of the virus in the host. The modified recombinant virus is used in a method for expressing a gene product in a host or in a cell cultured in vitro, and in a vaccine for inducing an immunological response in a host inoculated with the vaccine. What is also described is a selection system for the cloning and expression of open reading frames in poxviruses, particularly vaccinia virus. The selection system is based on a conThis invention was made with Government support under contract DAMD17-85-C-5232 awarded by the Department of the Army. The Government has certain rights in this invention.

    摘要翻译： 描述的是用于在宿主中表达基因产物的修饰的重组病毒。 修饰的重组病毒具有从其中缺失的宿主范围基因，使得病毒在宿主中具有限制性的复制。 修饰的重组病毒还含有编码并在宿主中表达基因产物的DNA，甚至在宿主中病毒的复制受限。 修饰的重组病毒用于在宿主或体外培养的细胞中表达基因产物的方法，以及用于在接种疫苗的宿主中诱导免疫应答的疫苗。 还描述了在痘病毒，特别是痘苗病毒中克隆和表达开放阅读框的选择系统。 选择系统基于痘病毒的条件致死突变体（宿主范围）。 产生痘苗病毒的缺失/重组突变体，其能够在原代小鸡胚胎成纤维细胞和两种猴细胞系（BSC-40或VERO）上进行染色，但在人细胞系MRC-5中复制缺陷。 将宿主范围基因插入缺失/重组体中恢复MRC-5细胞生长能力。 构建了一系列质粒，当与缺失/重组重组并在MRC-5细胞上进行生长时，允许快速单步克隆和任何开放阅读框的表达。

    Recombinant poxvirus internal cores
    38.
    发明授权
    Recombinant poxvirus internal cores 失效
    重组POXVIRUS内部线

    公开(公告)号：US5204243A

    公开(公告)日：1993-04-20

    申请号：US480097

    申请日：1990-02-14

    申请人： Enzo Paoletti

    发明人： Enzo Paoletti

    IPC分类号： A61K39/275 ,  A61K39/295 ,  C12N5/10 ,  C12N7/00 ,  C12N7/01 ,  C12N15/09 ,  C12N15/863 ,  C12P21/00 ,  C12R1/91 ,  C12R1/92

    CPC分类号： C12N7/00 ,  C12N15/86 ,  C12N2710/24143

    摘要： What is described is a modified recombinant poxvirus, such as vaccinia virus, fowlpox virus and canarypox virus, having an internal core containing DNA from a non-pox source in a nonessential region of the poxvirus genome. The recombinant poxvirus is modified by disassociating the internal core from outer membranes of the poxvirus. The DNA codes for and expresses a gene product in a cell cultured in vitro and in a host in vivo without productive replication of the virus in the cell or in the host. What is also described is a vaccine containing the modified recombinant poxvirus for inducing an immunological response in a host inoculated with the vaccine.

    摘要翻译： 描述的是修饰的重组痘病毒，例如痘苗病毒，禽痘病毒和金丝雀痘病毒，其内含核心含有来自痘病毒基因组非必需区域中非痘源的DNA。 通过将内部核心与痘病毒的外膜分离来修饰重组痘病毒。 该DNA编码并表达体外培养的细胞中的基因产物，并在体内宿主中表达基因产物，而在细胞或宿主中不产生病毒的复制。 还描述了含有用于在接种疫苗的宿主中诱导免疫应答的修饰重组痘病毒的疫苗。

    Recombinant poxvirus host range selection system
    40.
    发明授权
    Recombinant poxvirus host range selection system 失效
    重建POXVIRUS主机范围选择系统

    公开(公告)号：US5155020A

    公开(公告)日：1992-10-13

    申请号：US320471

    申请日：1989-03-08

    申请人： Enzo Paoletti

    发明人： Enzo Paoletti

    IPC分类号： A61K39/00 ,  C07K14/03 ,  C07K14/145 ,  C12N15/39 ,  C12N15/863

    CPC分类号： C12N15/86 ,  C07K14/005 ,  A61K39/00 ,  C12N2710/16722 ,  C12N2710/24143 ,  C12N2760/20122

    摘要： What is described is a selection system for the cloning and expression of open reading frames in poxviruses, particularly vaccinia virus. The selection system is based on a conditional lethal mutant (host range) of poxviruses. A deletion/recombinant mutant of the vaccinia virus was generated which is capable of plaquing on primary chick embryo fibroblasts and two monkey cell lines (BSC-40 or VERO) but is defective in replication in the human cell line MRC-5. Insertion of the host range gene into the deletion/recombinant restores the ability for growth on MCR-5 cells. A series of plasmids were constructed which allow for the rapid single-step cloning and expression of any open reading frame when recombined with the deletion/recombinant and scored for growth on MCR-5 cells.

    摘要翻译： 描述的是在痘病毒，特别是痘苗病毒中克隆和表达开放阅读框的选择系统。 选择系统基于痘病毒的条件致死突变体（宿主范围）。 产生痘苗病毒的缺失/重组突变体，其能够在原代小鸡胚胎成纤维细胞和两种猴细胞系（BSC-40或VERO）上进行染色，但是在人细胞系MRC-5中的复制缺陷。 将宿主范围基因插入缺失/重组中恢复MCR-5细胞上的生长能力。 构建了一系列质粒，当与缺失/重组重组并在MCR-5细胞上进行生长时，允许快速单步克隆和任何开放阅读框的表达。