公开(公告)号：US20050239132A1

公开(公告)日：2005-10-27

申请号：US10515535

申请日：2003-04-11

申请人： Holger Klapproth

发明人： Holger Klapproth

IPC分类号： B01L3/00 ,  G01N27/02 ,  G01N33/543 ,  G01N35/00 ,  G01N33/53

CPC分类号： G01N33/54373 ,  B01L3/5027 ,  G01N27/4145 ,  G01N2035/00158

摘要： The invention relates to a method for determining a concentration of ligands in an analyzed sample. The inventive method consists in immobilizing receptors specifically boundable with said ligands on a support. At least one measured value of the occupation of the support surface by the receptors is determined by means of a sensor for the surface occupation. Afterwards, the sample is put in contact with the receptors. At least one measured value of the frequency of liaisons between the ligands and the receptors is determined by means of at least one detector. The concentration of ligands in the sample can be determined with the aid of the measured values of the occupation of the surface and frequency of ligands/receptors liaisons.

摘要翻译： 本发明涉及一种用于测定分析样品中配体浓度的方法。 本发明的方法包括将与所述配体特异性结合的受体固定在载体上。 通过用于表面占据的传感器来确定由受体对支撑表面的占据的至少一个测量值。 然后将样品与受体接触。 通过至少一个检测器确定配体和受体之间的联络频率的至少一个测量值。 样品中配体的浓度可以借助于配体/受体结构的表面和频率的占据的测量值来确定。

公开(公告)号：US20050003557A1

公开(公告)日：2005-01-06

申请号：US10793323

申请日：2004-03-05

申请人： Holger Klapproth

发明人： Holger Klapproth

IPC分类号： G01N33/543

CPC分类号： G01N33/54353

摘要： A method for covalently immobilizing glycoproteins such as immunoglobulins on solid, semi-solid or gel-like surfaces, and constructs suitable therefor, as well as surfaces and devices that comprise correspondingly immobilized glycoproteins.

摘要翻译： 将固定，半固体或凝胶状表面上的糖蛋白如免疫球蛋白共价固定的方法及其适合的构建体，以及包含相应固定的糖蛋白的表面和装置。

公开(公告)号：US08518709B2

公开(公告)日：2013-08-27

申请号：US12619871

申请日：2009-11-17

申请人： Sonja Bednar ,  Johannes Baader ,  Holger Klapproth ,  Ingo Freund

发明人： Sonja Bednar ,  Johannes Baader ,  Holger Klapproth ,  Ingo Freund

IPC分类号： G01N21/00

CPC分类号： G01N33/54393

摘要： In a method for regenerating a biosensor, a biosensor is prepared having a substrate surface on which at least one receptor is immobilized. At least one ligand that is binding specific to the receptor is bound to the receptor, said ligand, together with the receptor, forming a ligand-receptor complex. To regenerate the biosensor, the ligand-receptor complex is brought into contact with an enzyme. The enzyme is selected so that it catalyzes the ligand into fragments. The enzyme is inert with respect to the receptor.

摘要翻译： 在用于再生生物传感器的方法中，制备具有固定有至少一种受体的底物表面的生物传感器。 与受体结合特异性的至少一种配体与受体结合，所述配体与受体一起形成配体 - 受体复合物。 为了再生生物传感器，使配体 - 受体复合物与酶接触。 选择该酶以使其将配体催化成片段。 该酶相对于受体是惰性的。

公开(公告)号：US07767443B2

公开(公告)日：2010-08-03

申请号：US10509040

申请日：2003-03-05

申请人： Mirko Lehmann ,  Holger Klapproth

发明人： Mirko Lehmann ,  Holger Klapproth

IPC分类号： C12M1/34 ,  C12M3/00

CPC分类号： C12M41/46

摘要： A device includes a carrier element with a surface prepared for direct or indirect coupling or receiving of cells, at least one optical detector to receive a luminescence signal, the detector being integrated into the carrier element below the prepared surface, a cover covering the prepared surface to form a cavity, the cover having an inlet opening and an outlet opening, and an excitation source connected to the inlet opening. The excitation source constitutes a reservoir for a chemical or biological excitation substance or medium that influences the metabolism of the cell, with metabolic processes being made visible by luminescence and detected by at the least one optical detector.

摘要翻译： 一种装置包括具有准备用于直接或间接耦合或接收细胞的表面的载体元件，至少一个用于接收发光信号的光学检测器，所述检测器被集成到所述制备表面下方的载体元件中，覆盖所制备的表面 为了形成空腔，盖具有入口开口和出口，以及连接到入口的激励源。 激发源构成用于影响细胞代谢的化学或生物激发物质或介质的储存器，代谢过程通过发光使其可见并且由至少一个光学检测器检测。

公开(公告)号：US20100003702A1

公开(公告)日：2010-01-07

申请号：US12558750

申请日：2009-09-14

申请人： Holger Klapproth

发明人： Holger Klapproth

IPC分类号： G01N33/542

CPC分类号： G01N33/54386 ,  B01F5/0603 ,  B01F13/0059 ,  B01L3/5027 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0487 ,  G01N21/6428

摘要： In a procedure for determining the concentrations of ligands contained in a sample wherein a first ligand is bond-specific to a first receptor and a second ligand is bond-specific to a second receptor, the first receptor is immobilized on a first test site and the second receptor is immobilized on a second test site on a substrate. A competitor that is bond-specific to the second receptor is mixed with the sample so that it is present in a predetermined concentration in the mixture. The mixture is brought into contact with the substrate so that the first ligand and the second ligand and/or the competitor bind to the first receptor and the second receptor, respectively. Afterwards, the unbound components of the mixture are removed from the substrate. A first metering signal relative to the concentration of the first ligand bound to the first receptor and a second metering signal relative to the concentration of the competitor bound to the second receptor are then generated. The concentrations of the ligands in the sample are determined with reference to the metering signals and the known concentration of the competitor in the mixture.

摘要翻译： 在确定样品中含有的配体浓度的步骤中，其中第一配体与第一受体结合 - 特异性，第二配体与第二受体结合 - 特异性，第一受体被固定在第一测试位点，并且 第二受体被固定在基底上的第二测试位点上。 将与第二受体结合的竞争剂与样品混合，使得其以混合物中预定浓度存在。 使混合物与底物接触，使得第一配体和第二配体和/或竞争剂分别与第一受体和第二受体结合。 之后，将混合物的未结合的组分从基材上除去。 然后产生相对于与第一受体结合的第一配体的浓度的第一计量信号和相对于与第二受体结合的竞争剂的浓度的第二计量信号。 参考测量信号和混合物中竞争者的已知浓度来确定样品中配体的浓度。

公开(公告)号：US07598044B2

公开(公告)日：2009-10-06

申请号：US11397347

申请日：2006-04-04

申请人： Holger Klapproth

发明人： Holger Klapproth

IPC分类号： G01N33/53

CPC分类号： G01N33/54386 ,  B01F5/0603 ,  B01F13/0059 ,  B01L3/5027 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2400/0487 ,  G01N21/6428

摘要： In a procedure for determining the concentrations of ligands contained in a sample wherein a first ligand is bond-specific to a first receptor and a second ligand is bond-specific to a second receptor, the first receptor is immobilized on a first test site and the second receptor is immobilized on a second test site on a substrate. A competitor that is bond-specific to the second receptor is mixed with the sample so that it is present in a predetermined concentration in the mixture. The mixture is brought into contact with the substrate so that the first ligand and the second ligand and/or the competitor bind to the first receptor and the second receptor, respectively. Afterwards the unbound components of the mixture are removed from the substrate. A first metering signal relative to the concentration of the first ligand bound to the first receptor and a second metering signal relative to the concentration of the competitor bound to the second receptor are then generated. The concentrations of the ligands in the sample are determined with reference to the metering signals and the known concentration of the competitor in the mixture.

摘要翻译： 在确定样品中含有的配体浓度的步骤中，其中第一配体与第一受体结合 - 特异性，第二配体与第二受体结合 - 特异性，第一受体被固定在第一测试位点，并且 第二受体被固定在基底上的第二测试位点上。 将与第二受体结合的竞争剂与样品混合，使得其以混合物中预定浓度存在。 使混合物与底物接触，使得第一配体和第二配体和/或竞争剂分别与第一受体和第二受体结合。 然后将混合物的未结合的组分从基底上除去。 然后产生相对于与第一受体结合的第一配体的浓度的第一计量信号和相对于与第二受体结合的竞争剂的浓度的第二计量信号。 参考测量信号和混合物中竞争者的已知浓度来确定样品中配体的浓度。

公开(公告)号：US20080287322A1

公开(公告)日：2008-11-20

申请号：US12183590

申请日：2008-07-31

申请人： Holger Klapproth ,  Mirko Lehmann

发明人： Holger Klapproth ,  Mirko Lehmann

IPC分类号： H01L21/44

CPC分类号： B01L3/0262 ,  B01J19/0046 ,  B01J2219/00382 ,  B01J2219/00385 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00605 ,  B01J2219/00612 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00635 ,  B01J2219/00637 ,  B01J2219/00644 ,  B01J2219/0072 ,  B01L3/5025 ,  B01L3/50857 ,  B01L2300/069 ,  B01L2300/0819 ,  B01L2400/022 ,  B01L2400/0406 ,  C40B60/14 ,  Y10T436/117497 ,  Y10T436/25

摘要： In a method for the manufacture of a microarray, surface areas of a chip are brought into contact with different aqueous solutions that contain at least one coating substance. For each of the individual surface areas, an absorbent substrate is provided and is filled with the solution that is to be brought into contact with the surface area in question. Then, for the printing of the surface area with the at least one coating substance contained in the solution, each substrate is brought into contact with the surface area in question.

摘要翻译： 在制造微阵列的方法中，芯片的表面积与含有至少一种涂层物质的不同水溶液接触。 对于每个单独的表面区域，提供吸收性基材，并填充与所述表面区域接触的溶液。 然后，为了用包含在溶液中的至少一种涂布物质印刷表面积，使每个基板与所讨论的表面区域接触。

公开(公告)号：US20070107514A1

公开(公告)日：2007-05-17

申请号：US11545778

申请日：2006-10-10

申请人： Ulrich Sieben ,  Holger Klapproth ,  Mirko Lehmann

发明人： Ulrich Sieben ,  Holger Klapproth ,  Mirko Lehmann

IPC分类号： G01F15/14

CPC分类号： G01N21/6454

摘要： An integrated sensor array has a substrates, at least one sensor element, and a signal processing device integrated in the substrate and connected or capable of being connected with the sensor elements. A layer having a measuring surface is capable of being detachably bonded to the substrate so that a signal emitted from the measuring surface is capable of being processed by the signal processing device when the layer is bonded to the substrate. The substrate has at least one feedthrough hole abutting with the layer, which feedthrough hole is connected or capable of being connected to a vacuum generating device in order to bond the layer to the substrate by suction.

摘要翻译： 集成传感器阵列具有基板，至少一个传感器元件和集成在基板中并连接或能够与传感器元件连接的信号处理装置。 具有测量表面的层能够可拆卸地结合到基板，使得当该层与基板结合时，从测量表面发射的信号能够被信号处理装置处理。 衬底具有与该层邻接的至少一个馈通孔，所述馈通孔连接或能够连接到真空产生装置，以便通过抽吸将层粘合到衬底。

公开(公告)号：US20060055042A1

公开(公告)日：2006-03-16

申请号：US10529048

申请日：2003-09-12

申请人： Holger Klapproth ,  Mirko Lehmann

发明人： Holger Klapproth ,  Mirko Lehmann

IPC分类号： H01L23/48

CPC分类号： G01N21/6454 ,  G01N21/6428 ,  G01N21/648 ,  H01L2924/0002 ,  H01L2924/00

摘要： Disclosed is a device for detecting at least one luminescent substance, comprising a radiation source for emitting excitation radiation to the at least one luminescent substance. The excitation radiation is provided with at least one excitation wavelength at which the luminescent substance is excited so as to emit luminescent radiation. At least one radiation receiver is provided, which detects the luminescent radiation and is configured regarding the spectral sensitivity thereof in such a way that said radiation receiver is insensitive to the radiation emitted by the radiation source. The luminescent substance is located inside a measuring chamber that is essentially impermeable to the luminescent radiation and comprises at least one wall area which is transparent to the excitation radiation emitted by the radiation source. The radiation source is placed outside the measuring chamber such that the excitation radiation emitted by the radiation source is coupled into the inside of the measuring chamber by penetrating said wall area.

摘要翻译： 公开了一种用于检测至少一种发光物质的装置，包括用于向至少一种发光物质发射激发辐射的辐射源。 激发辐射具有至少一个激发波长，在该激发波长处激发发光物质以发射发光辐射。 提供至少一个辐射接收器，其检测发光辐射，并且被配置为关于其光谱灵敏度，使得所述辐射接收器对由辐射源发射的辐射不敏感。 发光物质位于测量室内，其基本上不透气于发光辐射，并且包括对由辐射源发射的激发辐射透明的至少一个壁区域。 辐射源被放置在测量室的外部，使得由辐射源发射的激发辐射通过穿透所述壁区域而耦合到测量室的内部。

公开(公告)号：US20050153387A1

公开(公告)日：2005-07-14

申请号：US10509040

申请日：2003-03-05

申请人： Mirko Lehmann ,  Holger Klapproth

发明人： Mirko Lehmann ,  Holger Klapproth

IPC分类号： C12M1/34 ,  C12Q1/02 ,  G01N33/487 ,  G01N33/50 ,  G01N33/543 ,  G01N1/30 ,  G01N33/48

CPC分类号： C12M41/46

摘要： The invention relates to a device for detecting a luminescence event in or in the immediate vicinity of a cell, a cell cluster, or a tissue, having the following elements: (a) a carrier element (1) having a surface (100) prepared for direct or indirect coupling of cells, and (b) at least one optical detector (2) for receiving a luminescence signal and integrated in the carrier element (1) below the surface (100), characterized by the following elements: (c) a cover (7) having an inlet (8) and an outlet (9) and covering the surface (100) such as to form a cavity (70), and (d) an excitation source (21) connected to the inlet (8).

摘要翻译： 本发明涉及一种用于检测细胞，细胞簇或组织紧邻附近的发光事件的装置，具有以下元件：（a）具有制备表面（100）的载体元件（1） 用于单元的直接或间接耦合，以及（b）用于接收发光信号并集成在所述表面（100）下方的载体元件（1）中的至少一个光学检测器（2），其特征在于以下元件：（c） 具有入口（8）和出口（9）并覆盖所述表面（100）以形成空腔（70）的盖（7），和（d）连接到所述入口（8）的激发源 ）。