公开(公告)号：US20130338042A1

公开(公告)日：2013-12-19

申请号：US13783043

申请日：2013-03-01

Applicant: ILLUMINA, INC.

Inventor： Min-Jui Richard Shen ,  Jonathan Mark Boutell ,  Kathryn M. Stephens ,  Mostafa Ronaghi ,  Kevin Gunderson ,  Bala Murali Venkatesan ,  M. Shane Bowen ,  Kandaswamy Vijayan

IPC: B01J19/00

CPC classification number: C12Q1/6848 ,  B01J19/0046 ,  B01J2219/00585 ,  B01J2219/00587 ,  B01J2219/00608 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00675 ,  B01J2219/00722 ,  C12Q1/6844 ,  C12Q1/6874 ,  C12Q2521/507 ,  C12Q2527/101 ,  C12Q2527/113 ,  C12Q2535/122 ,  C12Q2565/513 ,  C12Q2565/537

Abstract: A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.

公开(公告)号：US11254976B2

公开(公告)日：2022-02-22

申请号：US16544670

申请日：2019-08-19

Applicant: Illumina, Inc.

Inventor： Min-Jui Richard Shen ,  Jonathan Mark Boutell ,  Kathryn M. Stephens ,  Mostafa Ronaghi ,  Kevin L. Gunderson ,  Bala Murali Venkatesan ,  M. Shane Bowen ,  Kandaswamy Vijayan

IPC: C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/6874 ,  B01J19/00

Abstract: A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.

公开(公告)号：US20200216895A1

公开(公告)日：2020-07-09

申请号：US16626115

申请日：2018-07-31

Applicant: ILLUMINA, INC.

Inventor： Tarun Kumar Khurana ,  Yir-Shyuan Wu ,  Xi-Jun Chen ,  Filiz Gorpe-Yasar ,  Yan-You Lin ,  Victoria Popic ,  Erich B. Jaeger ,  Mostafa Ronaghi

IPC: C12Q1/6874 ,  C12Q1/6813

Abstract: Implementations of a method for seeding sequence libraries on a surface of a sequencing flow cell that allow for spatial segregation of the libraries on the surface are provided. The spatial segregation can be used to index sequence reads from individual sequencing libraries to increase efficiency of subsequent data analysis. In some examples, hydrogel beads containing encapsulated sequencing libraries are captured on a sequencing flow cell and degraded in the presence of a liquid diffusion barrier to allow for the spatial segregation and seeding of the sequencing libraries on the surface of the flow cell. Additionally, examples of systems, methods and compositions are provided relating to flow cell devices configured for nucleic acid library preparation and single cell sequencing. Some examples include flow cell devices having a hydrogel with genetic material disposed therein, and which is retained within the hydrogel during nucleic acid processing.

公开(公告)号：US20160348170A1

公开(公告)日：2016-12-01

申请号：US15237480

申请日：2016-08-15

Applicant: ILLUMINA, INC.

Inventor： Mostafa Ronaghi ,  Helmy A. Eltoukhy

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6837 ,  C12Q1/6869 ,  C12Q2535/122 ,  C12Q2565/543

Abstract: The present technology relates to molecular sciences, such as genomics. More particularly, the present technology relates to methods for obtaining long lengths of sequencing data.

Abstract translation: 本技术涉及分子科学，如基因组学。 更具体地，本技术涉及用于获得长长度的测序数据的方法。

公开(公告)号：US08895249B2

公开(公告)日：2014-11-25

申请号：US13783043

申请日：2013-03-01

Applicant: Illumina, Inc.

Inventor： Min-Jui Richard Shen ,  Jonathan Mark Boutell ,  Kathryn M. Stephens ,  Mostafa Ronaghi ,  Kevin Gunderson ,  Bala Murali Venkatesan ,  M. Shane Bowen ,  Kandaswamy Vijayan

IPC: C12Q1/68 ,  C12P19/34 ,  B01J19/00

CPC classification number: C12Q1/6848 ,  B01J19/0046 ,  B01J2219/00585 ,  B01J2219/00587 ,  B01J2219/00608 ,  B01J2219/00621 ,  B01J2219/00637 ,  B01J2219/00653 ,  B01J2219/00675 ,  B01J2219/00722 ,  C12Q1/6844 ,  C12Q1/6874 ,  C12Q2521/507 ,  C12Q2527/101 ,  C12Q2527/113 ,  C12Q2535/122 ,  C12Q2565/513 ,  C12Q2565/537

Abstract: A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.

Abstract translation: 一种方法，包括（a）提供包含位点阵列的扩增试剂和具有不同靶核酸的溶液; 和（b）使扩增试剂反应以产生每个具有来自溶液的靶核酸的扩增子的克隆群的扩增位点。 反应可以包括以平均传输速率同时将核酸输送到位点，以及以平均扩增速率扩增转运到位点的核酸，其中平均扩增速率超过平均传输速率。 该反应可以包括从转运到每个位点的核酸产生第一扩增子，并产生来自核酸或第一扩增子的后续扩增子，其中后续扩增子产生的平均速率超过在 其中生成第一个扩增子。

公开(公告)号：US20140255919A1

公开(公告)日：2014-09-11

申请号：US14200674

申请日：2014-03-07

Applicant: Illumina, Inc.

Inventor： Mostafa Ronaghi ,  Helmy A. Eltoukhy

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6837 ,  C12Q1/6869 ,  C12Q2535/122 ,  C12Q2565/543

Abstract: The present technology relates to molecular sciences, such as genomics. More particularly, the present technology relates to methods for obtaining long lengths of sequencing data.

Abstract translation: 本技术涉及分子科学，如基因组学。 更具体地，本技术涉及用于获得长长度的测序数据的方法。