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公开(公告)号：US11254976B2
公开(公告)日：2022-02-22
申请号：US16544670
申请日：2019-08-19
Applicant: Illumina, Inc.
Inventor： Min-Jui Richard Shen , Jonathan Mark Boutell , Kathryn M. Stephens , Mostafa Ronaghi , Kevin L. Gunderson , Bala Murali Venkatesan , M. Shane Bowen , Kandaswamy Vijayan
IPC: C12Q1/6848 , C12Q1/6844 , C12Q1/6874 , B01J19/00
Abstract: A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.
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公开(公告)号：US20210108258A1
公开(公告)日：2021-04-15
申请号：US17113180
申请日：2020-12-07
Applicant: ILLUMINA, INC.
Inventor： M. Shane Bowen , Kevin L. Gunderson , Shengrong Lin , Maria Candelaria Rogert Bacigalupo , Kandaswamy Vijayan , Yir-Shyuan Wu , Bala Murali Venkatesan , James Tsay , John M. Beierle , Lorenzo Berti , Sang Ryul Park
IPC: C12Q1/6844 , C40B50/18 , C12Q1/686 , B01J19/00
Abstract: A method includes forming a patterned substrate including a plurality of base pads, using a nano-imprint lithography process. A capture substance is attached to each of the plurality of base pads, optionally through a linker, the capture substance being adapted to promote capture of a target molecule.
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公开(公告)号：US08895249B2
公开(公告)日：2014-11-25
申请号：US13783043
申请日：2013-03-01
Applicant: Illumina, Inc.
Inventor： Min-Jui Richard Shen , Jonathan Mark Boutell , Kathryn M. Stephens , Mostafa Ronaghi , Kevin Gunderson , Bala Murali Venkatesan , M. Shane Bowen , Kandaswamy Vijayan
CPC classification number: C12Q1/6848 , B01J19/0046 , B01J2219/00585 , B01J2219/00587 , B01J2219/00608 , B01J2219/00621 , B01J2219/00637 , B01J2219/00653 , B01J2219/00675 , B01J2219/00722 , C12Q1/6844 , C12Q1/6874 , C12Q2521/507 , C12Q2527/101 , C12Q2527/113 , C12Q2535/122 , C12Q2565/513 , C12Q2565/537
Abstract: A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.
Abstract translation: 一种方法，包括（a）提供包含位点阵列的扩增试剂和具有不同靶核酸的溶液; 和（b）使扩增试剂反应以产生每个具有来自溶液的靶核酸的扩增子的克隆群的扩增位点。 反应可以包括以平均传输速率同时将核酸输送到位点，以及以平均扩增速率扩增转运到位点的核酸，其中平均扩增速率超过平均传输速率。 该反应可以包括从转运到每个位点的核酸产生第一扩增子，并产生来自核酸或第一扩增子的后续扩增子，其中后续扩增子产生的平均速率超过在 其中生成第一个扩增子。
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公开(公告)号：US20220243261A1
公开(公告)日：2022-08-04
申请号：US17575279
申请日：2022-01-13
Applicant: Illumina, Inc.
Inventor： Min-Jui Richard Shen , Jonathan Mark Boutell , Kathryn M. Stephens , Mostafa Ronaghi , Kevin L. Gunderson , Bala Murali Venkatesan , M. Shane Bowen , Kandaswamy Vijayan
IPC: C12Q1/6848 , B01J19/00 , C12Q1/6844 , C12Q1/6874
Abstract: A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.
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公开(公告)号：US10385384B2
公开(公告)日：2019-08-20
申请号：US15672161
申请日：2017-08-08
Applicant: Illumina, Inc.
Inventor： Min-Jui Richard Shen , Jonathan Mark Boutell , Kathryn M. Stephens , Mostafa Ronaghi , Kevin L. Gunderson , Bala Murali Venkatesan , M. Shane Bowen , Kandaswamy Vijayan
IPC: C12Q1/68 , B01J19/00 , C12Q1/6848 , C12Q1/6844 , C12Q1/6874
Abstract: A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.
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Patent Agency Ranking
公开(公告)号：US11060135B2
公开(公告)日：2021-07-13
申请号：US16118268
申请日：2018-08-30
Applicant: ILLUMINA, INC.
Inventor： M. Shane Bowen , Kevin L. Gunderson , Shengrong Lin , Maria Candelaria Rogert Bacigalupo , Kandaswamy Vijayan , Yir-Shyuan Wu , Bala Murali Venkatesan , James Tsay , John M. Beierle , Lorenzo Berti , Sang Ryul Park
IPC: C12Q1/6844 , C40B50/18 , C12Q1/686 , B01J19/00 , C40B40/06
Abstract: A method includes forming a patterned substrate including a plurality of base pads, using a nano-imprint lithography process. A capture substance is attached to each of the plurality of base pads, optionally through a linker, the capture substance being adapted to promote capture of a target molecule.
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公开(公告)号：US20150080230A1
公开(公告)日：2015-03-19
申请号：US14512693
申请日：2014-10-13
Applicant: ILLUMINA, INC.
Inventor： Min-Jui Richard Shen , Jonathan Mark Boutell , Kathryn M. Stephens , Mostafa Ronaghi , Kevin Gunderson , Bala Murali Venkatesan , M. Shane Bowen , Kandaswamy Vijayan
CPC classification number: C12Q1/6848 , B01J19/0046 , B01J2219/00585 , B01J2219/00587 , B01J2219/00608 , B01J2219/00621 , B01J2219/00637 , B01J2219/00653 , B01J2219/00675 , B01J2219/00722 , C12Q1/6844 , C12Q1/6874 , C12Q2521/507 , C12Q2527/101 , C12Q2527/113 , C12Q2535/122 , C12Q2565/513 , C12Q2565/537
Abstract: A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.
Abstract translation: 一种方法，包括（a）提供包含位点阵列的扩增试剂和具有不同靶核酸的溶液; 和（b）使扩增试剂反应以产生每个具有来自溶液的靶核酸的扩增子的克隆群的扩增位点。 反应可以包括以平均传输速率同时将核酸输送到位点，以及以平均扩增速率扩增转运到位点的核酸，其中平均扩增速率超过平均传输速率。 该反应可以包括从转运到每个位点的核酸产生第一扩增子，并产生来自核酸或第一扩增子的后续扩增子，其中后续扩增子产生的平均速率超过在 其中生成第一个扩增子。
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公开(公告)号：US20130116153A1
公开(公告)日：2013-05-09
申请号：US13661524
申请日：2012-10-26
Applicant: ILLUMINA, INC.
Inventor： M. Shane Bowen , Kevin L. Gunderson , Shengrong Lin , Maria Candelaria Rogert Bacigalupo , Kandaswamy Vijayan , Yir-Shyuan Wu , Bala Murali Venkatesan , James Tsay , John M. Beierle , Lorenzo Berti , Sang Ryul Park
IPC: B01J19/00
CPC classification number: C12Q1/6844 , B01J19/0046 , B01J2219/00529 , B01J2219/00608 , B01J2219/00621 , B01J2219/00637 , B01J2219/00641 , B01J2219/00644 , B01J2219/00648 , B01J2219/00653 , B01J2219/00659 , B01J2219/00662 , B01J2219/00722 , B01J2219/00788 , C12Q1/686 , C40B50/18 , C12Q2527/125 , C12Q2535/122 , C12Q2565/543
Abstract: A microarray is designed capture one or more molecules of interest at each of a plurality of sites on a substrate. The sites comprise base pads, such as polymer base pads, that promote the attachment of the molecules at the sites. The microarray may be made by one or more patterning techniques to create a layout of base pads in a desired pattern. Further, the microarrays may include features to encourage clonality at the sites.
Abstract translation: 设计的微阵列在基底上的多个位点的每一个处捕获一个或多个感兴趣的分子。 这些部位包括促进分子在部位附着的基底垫，例如聚合物基底垫。 微阵列可以通过一种或多种图案化技术制成，以产生所需图案中的基底布局。 此外，微阵列可以包括在站点鼓励克隆性的特征。
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公开(公告)号：US20200040386A1
公开(公告)日：2020-02-06
申请号：US16544670
申请日：2019-08-19
Applicant: Illumina, Inc.
Inventor： Min-Jui Richard Shen , Jonathan Mark Boutell , Kathryn M. Stephens , Mostafa Ronaghi , Kevin L. Gunderson , Bala Murali Venkatesan , M. Shane Bowen , Kandaswamy Vijayan
IPC: C12Q1/6848 , B01J19/00 , C12Q1/6844 , C12Q1/6874
Abstract: A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.
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公开(公告)号：US09758816B2
公开(公告)日：2017-09-12
申请号：US14879932
申请日：2015-10-09
Applicant: Illumina, Inc.
Inventor： Min-Jui Richard Shen , Jonathan Mark Boutell , Kathryn M. Stephens , Mostafa Ronaghi , Kevin Gunderson , Bala Murali Venkatesan , M. Shane Bowen , Kandaswamy Vijayan
CPC classification number: C12Q1/6848 , B01J19/0046 , B01J2219/00585 , B01J2219/00587 , B01J2219/00608 , B01J2219/00621 , B01J2219/00637 , B01J2219/00653 , B01J2219/00675 , B01J2219/00722 , C12Q1/6844 , C12Q1/6874 , C12Q2521/507 , C12Q2527/101 , C12Q2527/113 , C12Q2535/122 , C12Q2565/513 , C12Q2565/537
Abstract: A method including (a) providing an amplification reagent including an array of sites, and a solution having different target nucleic acids; and (b) reacting the amplification reagent to produce amplification sites each having a clonal population of amplicons from a target nucleic acid from the solution. The reacting can include simultaneously transporting the nucleic acids to the sites at an average transport rate, and amplifying the nucleic acids that transport to the sites at an average amplification rate, wherein the average amplification rate exceeds the average transport rate. The reacting can include producing a first amplicon from a nucleic acid that transports to each of the sites, and producing subsequent amplicons from the nucleic acid or from the first amplicon, wherein the average rate at which the subsequent amplicons are generated exceeds the average rate at which the first amplicon is generated.
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