摘要:
Embodiments in accordance with the present invention relate to packed-column nano-liquid chromatography (nano-LC) systems integrated on-chip, and methods for producing and using same. The microfabricated chip includes a column, flits/filters, an injector, and a detector, fabricated in a process compatible with those conventionally utilized to form integrated circuits. The column can be packed with supports for various different stationary phases to allow performance of different forms of nano-LC, including but not limited to reversed-phase, normal-phase, adsorption, size-exclusion, affinity, and ion chromatography. A cross-channel injector injects a nanolitre/picolitre-volume sample plug at the column inlet. An electrochemical/conductivity sensor integrated at the column outlet measures separation signals. A self-aligned channel-strengthening technique increases pressure rating of the microfluidic system, allowing it to withstand the high pressure normally used in high performance liquid chromatography (HPLC). On-chip sample injection, separation, and detection of mixture of anions in water is successfully demonstrated using ion-exchange nano-LC.
摘要:
A diagnostic device is provided that includes a plurality of retainment regions, with the retainment regions that are separated by at least one dissolvable barrier. The retainment regions can be interconnected through at least one fluid processing passageway. A retainment region can include a container such as a retainment region, well, chamber, or other receptacle, or a retainment region such as a surface on which the material is retained. The retainment regions can include a reaction retainment region, one or more reagent retainment regions, each containing unreacted reagents, and a sample retainment region. A pressure-actuated valve can be positioned in each fluid processing passageway interconnecting the one or more reagent retainment regions with the respective intermediate retainment regions interposed between each of the one or more reagent retainment regions and the reaction retainment region. The dissolvable barrier can be a fluid flow modulator in the at least one fluid processing passageway.
摘要:
In one embodiment, detecting a wireless network access request, forwarding data associated with the detected wireless network access request to a first multipoint Generic Routing Encapsulation (mGRE) tunnel, receiving authentication information associated with the detected wireless network access request, receiving authentication status information for the detected wireless network access request, and forwarding data associated with the detected wireless network access request to a second multipoint Generic Routing Encapsulation (mGRE) tunnel connected to a predetermined internet protocol (IP) subnet when the received authentication status information includes a successful authentication, are provided.
摘要:
An apparatus for liquid chromatography comprises a liquid chromatography separation column on a substrate, wherein the separation column is coupled to a heater on the substrate. A chip-based temperature controlled liquid chromatography device comprises a substrate, a thermal isolation zone, and a separation column thermally isolated from the substrate by the thermal isolation zone. An apparatus for chip-based liquid chromatography comprising a cooling device is provided. A temperature gradient liquid chromatography system comprises a chip-based temperature controlled liquid chromatography device, a fluidic coupling, and an electrical interface. Methods of making and methods of using of chip-based temperature gradient liquid chromatography devices are also provided.
摘要:
In one embodiment, detecting a wireless network access request, forwarding data associated with the detected wireless network access request to a first multipoint Generic Routing Encapsulation (mGRE) tunnel, receiving authentication information associated with the detected wireless network access request, receiving authentication status information for the detected wireless network access request, and forwarding data associated with the detected wireless network access request to a second multipoint Generic Routing Encapsulation (mGRE) tunnel connected to a predetermined internet protocol (IP) subnet when the received authentication status information includes a successful authentication, are provided.
摘要:
A microfluidic device and method for capacitive sensing. The device includes a fluid channel including an inlet at a first end and an outlet at a second end, a cavity region coupled to the fluid channel, and a polymer based membrane coupled between the fluid channel and the cavity region. Additionally, the device includes a first capacitor electrode coupled to the membrane, a second capacitor electrode coupled to the cavity region and physically separated from the first capacitor electrode by at least the cavity region, and an electrical power source coupled between the first capacitor electrode and the second capacitor electrode and causing an electric field at least within the cavity region. The polymer based membrane includes a polymer.
摘要:
A two-step high density plasma-CVD process is described wherein the argon content in the film is controlled by using two different argon concentrations in the argon/silane/oxygen gas mixture used for generating the high density plasma. The first step deposition uses high argon concentration and low sputter etch-to-deposition (E/D) ratio. High E/D ratio maintains the gap openings without necking. In the second step, a lower argon concentration and lower E/D ratio are used. Since observed metal defects are caused by argon diffusion in the top 200-300 nm of the HDP-CVD film, by controlling argon concentration in the top part of the film (i.e. second step deposition) to a low value, a reduced number of metal defects are achieved.
摘要:
A microfluidic device and method for capacitive sensing. The device includes a fluid channel including an inlet at a first end and an outlet at a second end, a cavity region coupled to the fluid channel, and a polymer based membrane coupled between the fluid channel and the cavity region. Additionally, the device includes a first capacitor electrode coupled to the membrane, a second capacitor electrode coupled to the cavity region and physically separated from the first capacitor electrode by at least the cavity region, and an electrical power source coupled between the first capacitor electrode and the second capacitor electrode and causing an electric field at least within the cavity region. The polymer based membrane includes a polymer.
摘要:
Antibodies and meditopes that bind to the antibodies are provided, as well as complexes, compositions and combinations containing the meditopes and antibodies, and methods of producing, using, testing, and screening the same, including therapeutic and diagnostic methods and uses.
摘要:
A method and device for recording data of a terminal are disclosed. The method includes: setting a relation condition list for triggering recording among function modules, and setting a corresponding random probability generator for each function module; determining whether to start recording data, and whether to end the data recording operation in accordance with the relation condition list or the random probability generator, during the operation process of each function module; and sending the respective data packets obtained by recording to a data management module for storage after the data record operation of each function module is ended. By adopting the method and device, fuzzy recording can be performed on data of the terminal, and data of different function modules can be stored centrally, and a query operation is simplified.