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110 条记录 (耗时 0.023 秒)

    Method for producing dipeptide
    31.
    发明授权
    Method for producing dipeptide 失效
    二肽生产方法

    公开(公告)号：US07115389B2

    公开(公告)日：2006-10-03

    申请号：US11008203

    申请日：2004-12-10

    申请人： Seiichi Hara ,  Kenzo Yokozeki

    发明人： Seiichi Hara ,  Kenzo Yokozeki

    IPC分类号： C12P21/06

    CPC分类号： C07K5/06026 ,  C12P21/02 ,  Y10S435/823 ,  Y10S435/849

    摘要： A method for producing a dipeptide including the step of exposing a carboxy component and an amine component to an enzyme having an activity to hydrolyze amino acid ester. Such a hydrolase has been found among enzymes in bacteria. The method is useful for producing a peptide simply and inexpensively with a high yield without taking complicate synthetic methods.

    摘要翻译： 一种制备二肽的方法，包括将羧基组分和胺组分暴露于具有水解氨基酸酯活性的酶的步骤。 这种水解酶已经在细菌的酶中发现。 该方法可用于在不采取复杂的合成方法的情况下以高产率简单且廉价地生产肽。

    Method for producing dipeptide
    33.
    发明申请
    Method for producing dipeptide 失效
    二肽生产方法

    公开(公告)号：US20050186654A1

    公开(公告)日：2005-08-25

    申请号：US11008203

    申请日：2004-12-10

    申请人： Seiichi Hara ,  Kenzo Yokozeki

    发明人： Seiichi Hara ,  Kenzo Yokozeki

    IPC分类号： C12N15/09 ,  C07K1/06 ,  C07K5/06 ,  C12P21/02 ,  C12P21/06 ,  C07C237/10 ,  C07K5/04

    CPC分类号： C07K5/06026 ,  C12P21/02 ,  Y10S435/823 ,  Y10S435/849

    摘要： A method for producing a dipeptide including the step of exposing a carboxy component and an amine component to an enzyme having an activity to hydrolyze amino acid ester. Such a hydrolase has been found among enzymes in bacteria. The method is useful for producing a peptide simply and inexpensively with a high yield without taking complicate synthetic methods.

    摘要翻译： 一种制备二肽的方法，包括将羧基组分和胺组分暴露于具有水解氨基酸酯活性的酶的步骤。 这种水解酶已经在细菌的酶中发现。 该方法可用于在不采取复杂的合成方法的情况下以高产率简单且廉价地生产肽。

    Bacterium for the production of 2′-deoxyribonucleoside
    34.
    发明授权
    Bacterium for the production of 2′-deoxyribonucleoside 失效
    用于生产2'-脱氧核糖核苷的细菌

    公开(公告)号：US07223589B2

    公开(公告)日：2007-05-29

    申请号：US10879305

    申请日：2004-06-30

    申请人： Seiichi Hara ,  Naoto Tonouchi ,  Kenzo Yokozeki

    发明人： Seiichi Hara ,  Naoto Tonouchi ,  Kenzo Yokozeki

    IPC分类号： C12N1/20 ,  C12P1/00 ,  C07H21/04

    CPC分类号： C12P19/38 ,  C12P19/40

    摘要： The present invention provides a microorganism for producing a 2′-deoxyribonucleoside. In particular, the microorganism of the present invention is transformed with a gene encoding a ribonucleotide reductase and in which 2′-deoxyribonucleoside degradation activity is decreased or eliminated by disrupting a gene encoding a purine nucleoside phosphorylase on chromosomal DNA.

    摘要翻译： 本发明提供了用于制备2'-脱氧核糖核苷的微生物。 特别地，本发明的微生物用编码核糖核苷酸还原酶的基因转化，通过在染色体DNA上破坏编码嘌呤核苷磷酸化酶的基因来降低或消除2'-脱氧核糖核苷降解活性。

    Novel peptide-forming enzyme, microbe producing the enzyme and method for producing peptide using them
    36.
    发明申请
    Novel peptide-forming enzyme, microbe producing the enzyme and method for producing peptide using them 审中-公开
    新型肽形成酶，生产酶的微生物和使用它们生产肽的方法

    公开(公告)号：US20050032187A1

    公开(公告)日：2005-02-10

    申请号：US10775178

    申请日：2004-02-11

    申请人： Kenzo Yokozeki ,  Sonoko Suzuki ,  Seiichi Hara ,  Satoshi Katayama

    发明人： Kenzo Yokozeki ,  Sonoko Suzuki ,  Seiichi Hara ,  Satoshi Katayama

    IPC分类号： C12N1/21 ,  C12N9/00 ,  C12N9/52 ,  C12P21/02 ,  C12P21/06 ,  C07H21/04

    CPC分类号： C12N9/93 ,  C12N9/52 ,  C12P21/02 ,  C12R1/01

    摘要： The present invention relates to a novel enzyme that allows peptide to be produced easily, inexpensively and at high yield without going through a complex synthesis method. More particularly, the present invention provides a novel enzyme that catalyzes a peptide-producing reaction from a carboxy component and an amine component, a microbe that produces the enzyme, and a method for inexpensive production of peptides using this enzyme or microbe. The novel enzyme that efficiently produces peptide was discovered from a newly discovered microbe belonging to the genus Empedobacter, and a method was found that allows peptides to be produced inexpensively and easily.

    摘要翻译： 本发明涉及一种能够容易地，低成本地以高产率生产肽而不经过复合合成方法的新型酶。 更具体地说，本发明提供了一种从羧基组分和胺组分催化产生肽的反应的新型酶，产生酶的微生物，以及使用该酶或微生物廉价生产肽的方法。 从新发现的属于Empedobacter属的微生物发现有效产生肽的新型酶，并且发现能够廉价且容易地生产肽的方法。

    Method for producing 2′ -deoxyribonucleoside
    37.
    发明授权
    Method for producing 2′ -deoxyribonucleoside 失效
    2'-脱氧核糖核苷的制备方法

    公开(公告)号：US06777208B2

    公开(公告)日：2004-08-17

    申请号：US09983657

    申请日：2001-10-25

    申请人： Seiichi Hara ,  Naoto Tonouchi ,  Kenzo Yokozeki

    发明人： Seiichi Hara ,  Naoto Tonouchi ,  Kenzo Yokozeki

    IPC分类号： C12P1938

    CPC分类号： C12P19/38 ,  C12P19/40

    摘要： A 2′-deoxyribonucleoside is produced by culturing a microorganism, which is transformed with a gene encoding a ribonucleotide reductase and in which 2′-deoxyribonucleoside degradation activity is decreased or eliminated by disrupting a gene encoding a purine nucleoside phosphorylase on chromosomal DNA, in a medium in which the microorganism can grow to produce the 2′-deoxyribonucleoside.

    摘要翻译： 通过培养用编码核糖核苷酸还原酶的基因转化的微生物，通过在染色体DNA上破坏编码嘌呤核苷磷酸化酶的基因而降低或消除2'-脱氧核糖核苷降解活性的2'-脱氧核糖核苷 其中微生物能够生长以产生2'-脱氧核糖核苷的培养基。

    METHOD FOR PRODUCING SERINE DERIVATIVE AND PROTEIN USED FOR THE SAME
    39.
    发明申请
    METHOD FOR PRODUCING SERINE DERIVATIVE AND PROTEIN USED FOR THE SAME 有权
    生产用于其的丝氨酸衍生物和蛋白质的方法

    公开(公告)号：US20100317068A1

    公开(公告)日：2010-12-16

    申请号：US12836984

    申请日：2010-07-15

    申请人： Shinji Kuroda ,  Masakazu Sugiyama ,  Kunihiko Watanabe ,  Shunichi Suzuki ,  Kenzo Yokozeki ,  Tatsuki Kashiwagi

    发明人： Shinji Kuroda ,  Masakazu Sugiyama ,  Kunihiko Watanabe ,  Shunichi Suzuki ,  Kenzo Yokozeki ,  Tatsuki Kashiwagi

    IPC分类号： C12P13/06 ,  C07H21/00 ,  C12N9/10 ,  C12N1/21

    CPC分类号： C12N9/1029 ,  C12P13/04

    摘要： The present invention describes a method for generating a serine derivative and an optically active isomer thereof by a convenient technique, and an enzyme and the like useful in the method. In the presence of the following protein (A) and/or (B) having an enzymatic activity, an α-amino acid is reacted with an aldehyde to form a serine derivative: (A) a protein comprising the amino acid sequence of SEQ ID NO:5, and (B) a protein comprising an amino acid sequence of SEQ ID NO: 5, but which includes substitution, deletion, insertion and addition of one or more amino acids and is able to catalyze the reaction to form the serine derivative.

    摘要翻译： 本发明描述了通过方便的技术产生丝氨酸衍生物和光学活性异构体的方法，以及在该方法中有用的酶等。 在具有酶活性的下列蛋白质（A）和/或（B）的存在下，α-氨基酸与醛反应形成丝氨酸衍生物：（A）包含SEQ ID NO：1的氨基酸序列的蛋白质 （B）包含SEQ ID NO：5的氨基酸序列但包括取代，缺失，插入和加入一个或多个氨基酸的蛋白质，并且能够催化该反应以形成丝氨酸衍生物 。