公开(公告)号：US10407676B2

公开(公告)日：2019-09-10

申请号：US14964060

申请日：2015-12-09

Applicant: LIFE TECHNOLOGIES CORPORATION ,  THERMO FISHER SCIENTIFIC GENEART GMBH ,  LIFE TECHNOLOGIES AS

Inventor： Thomas Poehmerer ,  Phillip Kuhn ,  Frank Notka ,  Andreas Zeidler ,  Korbinian Heil ,  Axel Trefzer ,  Geir Fonnum ,  Federico Katzen ,  Kristian Andersson

IPC: C12N15/10 ,  C25B3/10 ,  C25B11/04 ,  C25B15/02 ,  C25B9/18 ,  B01L3/00 ,  B03C5/02

Abstract: The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid molecules, optionally followed by assembly of these nucleic acid molecules into larger molecules. In some aspects, the invention allows for efficient production of nucleic acid molecules (e.g., large nucleic acid molecules such as genomes).

公开(公告)号：US10202473B2

公开(公告)日：2019-02-12

申请号：US15005420

申请日：2016-01-25

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Grete Irene Modahl ,  Nini Hofsloekken Kjus ,  Astrid Evenroed Molteberg ,  Diem Thuy Thi Tran ,  Jo Aaserud ,  M. Talha Gokmen ,  Steven M. Menchen ,  Carl Fuller ,  Luisa Andruzzi ,  Wolfgang Hinz

IPC: C08F130/08 ,  C08F6/24 ,  C07F7/18 ,  C08F2/20 ,  C08F2/26 ,  C08F220/58 ,  C08F230/08 ,  C08F222/38 ,  C08F8/32 ,  C08F8/34 ,  C12Q1/686 ,  C12P19/34 ,  C12Q1/6874 ,  G01N27/414 ,  C08J9/20 ,  C08F8/12

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

公开(公告)号：US09909165B2

公开(公告)日：2018-03-06

申请号：US15431678

申请日：2017-02-13

Applicant: LIFE TECHNOLOGIES AS

Inventor： Darren Ellis ,  Geir Fonnum ,  Nini Kjus

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6806 ,  C12N15/1006 ,  C12N15/1013

Abstract: A method for processing a nucleic acid, in which the nucleic acid is exposed to an aqueous medium which includes a polyol in sufficient proportion for at least a portion of the nucleic acid to enter or remain in an extra-solution phase. Thus, a polyol may be used to bind a nucleic acid which is in solution to a solid support or to wash a nucleic acid on a solid support while maintaining it on the support. The polyol may for example be a C2-C10 alkanediol.

公开(公告)号：US09487603B2

公开(公告)日：2016-11-08

申请号：US14970241

申请日：2015-12-15

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Grete Irene Modahl ,  Nini Hofslokken Kjus ,  Astrid Evenroed Molteberg ,  Diem Tran ,  Jo Aaserud ,  M. Talha Gokmen ,  Steven M. Menchen ,  Carl Fuller ,  Luisa Andruzzi ,  Wolfgang Hinz

IPC: C08F130/08 ,  C08F6/24 ,  C08J9/20 ,  C07F7/18 ,  C08F2/20 ,  C08F2/26 ,  C08F220/58 ,  C08F230/08 ,  C08F222/38 ,  C08F8/32 ,  C08F8/34 ,  C12Q1/68 ,  C12P19/34 ,  G01N27/414

CPC classification number: C08F130/08 ,  C07F7/1804 ,  C07F7/188 ,  C08F2/20 ,  C08F2/26 ,  C08F6/24 ,  C08F8/12 ,  C08F8/32 ,  C08F8/34 ,  C08F220/58 ,  C08F222/385 ,  C08F230/08 ,  C08F2800/20 ,  C08J9/20 ,  C08J2343/04 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N27/4145 ,  Y10T428/2982 ,  C08F257/02 ,  C08F2222/1013 ,  C08F212/36 ,  C08F220/60 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/543

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

公开(公告)号：US09243085B2

公开(公告)日：2016-01-26

申请号：US13762941

申请日：2013-02-08

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Grete Modahl ,  Nini Kjus ,  Astrid Evenroed Molteberg ,  Diem Tran ,  Jo Aaserud ,  M. Talha Gokmen ,  Steven Menchen ,  Carl Fuller ,  Luisa Andruzzi ,  Wolfgang Hinz

IPC: C08F2/16 ,  C08F230/08 ,  C08F8/34 ,  C08F6/24 ,  C07F7/18 ,  C08F130/08 ,  C08F2/20 ,  C08F2/26 ,  C08F220/58 ,  C08F222/38 ,  C08F8/32 ,  C12Q1/68

CPC classification number: C08F130/08 ,  C07F7/1804 ,  C07F7/188 ,  C08F2/20 ,  C08F2/26 ,  C08F6/24 ,  C08F8/12 ,  C08F8/32 ,  C08F8/34 ,  C08F220/58 ,  C08F222/385 ,  C08F230/08 ,  C08F2800/20 ,  C08J9/20 ,  C08J2343/04 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N27/4145 ,  Y10T428/2982 ,  C08F257/02 ,  C08F2222/1013 ,  C08F212/36 ,  C08F220/60 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/543

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

公开(公告)号：US09187691B2

公开(公告)日：2015-11-17

申请号：US14190054

申请日：2014-02-25

Applicant: LIFE TECHNOLOGIES AS

Inventor： Ellen Weng ,  Geir Fonnum ,  Grete Modahl ,  Astrid Molteberg ,  Torkel Stene

IPC: G01N33/553 ,  G01N33/544 ,  C09K11/06 ,  B82Y25/00 ,  B82Y40/00 ,  G01N33/543 ,  H01F1/01 ,  H01F41/00 ,  C09K11/02 ,  H01F1/00 ,  H01F1/26 ,  C08F220/18

CPC classification number: C09K11/06 ,  B82Y25/00 ,  B82Y40/00 ,  C08F220/18 ,  C09K11/025 ,  G01N33/5434 ,  H01F1/0018 ,  H01F1/01 ,  H01F1/26 ,  H01F41/00 ,  Y10T428/2991 ,  Y10T428/2998

Abstract: A coated magnetic particle comprising an optionally porous magnetic polymer particle of a matrix polymer, said polymer particle having on a surface and/or in the pores thereof superparamagnetic crystals, said coated particle having a coat formed of a coating polymer, wherein said coated magnetic particle is essentially non-autofluorescent.

Abstract translation: 一种涂覆的磁性颗粒，其包含基质聚合物的任选多孔磁性聚合物颗粒，所述聚合物颗粒在表面上和/或其孔中具有超顺磁性晶体，所述涂覆颗粒具有由涂覆聚合物形成的涂层，其中所述涂覆的磁性颗粒 本质上是非自发荧光。

公开(公告)号：US09156925B2

公开(公告)日：2015-10-13

申请号：US14082419

申请日：2013-11-18

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Geir Fonnum ,  Grete I. Modahl ,  Nini H. Kjus ,  Astrid E. Molteberg ,  Diem Tran ,  Jo Aaserud ,  Talha M. Gokmen ,  Steven M. Menchen ,  Carl Fuller ,  Luisa Andruzzi ,  Wolfgang Hinz

IPC: C08F6/24 ,  C08F8/34 ,  C08F2/20 ,  C08F2/26 ,  C07F7/18 ,  C08F130/08 ,  C08F220/58 ,  C08F230/08 ,  C08F222/38 ,  C08F8/32 ,  C12Q1/68

CPC classification number: C08F130/08 ,  C07F7/1804 ,  C07F7/188 ,  C08F2/20 ,  C08F2/26 ,  C08F6/24 ,  C08F8/12 ,  C08F8/32 ,  C08F8/34 ,  C08F220/58 ,  C08F222/385 ,  C08F230/08 ,  C08F2800/20 ,  C08J9/20 ,  C08J2343/04 ,  C12P19/34 ,  C12Q1/686 ,  C12Q1/6874 ,  G01N27/4145 ,  Y10T428/2982 ,  C08F257/02 ,  C08F2222/1013 ,  C08F212/36 ,  C08F220/60 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/543

Abstract: A method of forming a particle includes, in a disperse phase within an aqueous suspension, polymerizing a plurality of mer units of a hydrophilic monomer having a hydrophobic protection group, thereby forming a polymeric particle including a plurality of the hydrophobic protection groups. The method further includes converting the polymeric particle to a hydrophilic particle.

公开(公告)号：US09062079B2

公开(公告)日：2015-06-23

申请号：US13762489

申请日：2013-02-08

Applicant: LIFE TECHNOLOGIES CORPORATION ,  LIFE TECHNOLOGIES AS

Inventor： Nini Kjus ,  Geir Fonnum ,  Bruce Branchaud ,  Lai-Qiang Ying ,  Steven Menchen ,  Dmitriy Gremyachinskiy ,  Hee Chol Kang

IPC: C07F7/18 ,  C07F7/10 ,  C08F222/38 ,  C08F220/58 ,  C08F236/20 ,  C08K3/20

CPC classification number: C08F230/08 ,  C07F7/10 ,  C07F7/1804 ,  C08F220/58 ,  C08F222/385 ,  C08F236/20 ,  C08K3/20

Abstract: A silyl protected diacrylamide compound is described. A method of forming such a compound includes mixing a silylation reagent with a hydroxylated diamine compound under first reactive conditions to form a product in a first solution, separating the product from the first solution, and mixing the product with acryloyl chloride under second reactive conditions in a second solution to form a silyl protected diacrylamide compound.

Abstract translation: 描述了甲硅烷基保护的二丙烯酰胺化合物。 形成这种化合物的方法包括在第一反应条件下将甲硅烷基化试剂与羟基化二胺化合物混合以在第一溶液中形成产物，将产物与第一溶液分离，并在第二反应条件下将产物与丙烯酰氯混合 形成甲硅烷基保护的二丙烯酰胺化合物的第二溶液。