公开(公告)号：US20230101252A1

公开(公告)日：2023-03-30

申请号：US18077404

申请日：2022-12-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： John NOBILE ,  George Thomas ROTH ,  Todd REARICK ,  Jonathan SCHULTZ ,  Jonathan M. ROTHBERG ,  David MARRAN

IPC: G01N27/30 ,  B01L3/00 ,  G01N27/414

Abstract: An apparatus includes a reaction vessels coupled to an electronic sensor for monitoring a reaction product in the reaction vessel; a fluidics system for sequentially delivering a plurality of reagents to the reaction vessel, the fluidics system including a plurality of reagent reservoirs in fluidic communication via a plurality of flow paths with a fluidics circuit and to a common passage in fluidic communication between the fluidics circuit and the reaction vessel, a solution reservoir in fluidic communication with the common passage via a branch passage connected with the common passage at a junction between the fluidics circuit and the reaction vessel; and an electrode in contact with a solution within the branch passage, the electrode being in electrical communication with the reaction vessel through fluid extending from the branch passage and through the common passage, the electronic sensor generating an output signal depending on a voltage of the electrode.

公开(公告)号：US20220185653A1

公开(公告)日：2022-06-16

申请号：US17559440

申请日：2021-12-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Todd ROSWECH ,  Jonathan SCHULTZ ,  Chun HO

IPC: B67D7/02 ,  B67D7/76 ,  B01L3/00

Abstract: A fluidic interconnect includes a first interface including a liquid port, a gas port, and a cradle; a second interface including a liquid port, a gas port, and a swing bar to engage the cradle, a weight of a container attached to one of the first or second interfaces to drive the liquid port of the first interface into connection with the liquid port of the second interface and the gas port of the first interface into connection with the gas port of the second interface.

公开(公告)号：US20220057413A1

公开(公告)日：2022-02-24

申请号：US17465303

申请日：2021-09-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James A. BALL ,  Jonathan SCHULTZ

IPC: G01N33/84 ,  C12Q1/6869 ,  B01J19/00

Abstract: A buffered suspension includes a surfactant and a solid buffer particulate having a point of zero charge at least 1.2 pH units different that the pH of the buffered suspension. The buffered suspension can be prepared by mixing a stock solution with the solid buffer particulate and titrating. A method of preforming a pH sensitive process includes drawing the buffered suspension from a reservoir, filtering the solid buffer particulate from the buffered suspension, and applying the filtered solution to a sensor.

公开(公告)号：US20210155466A1

公开(公告)日：2021-05-27

申请号：US17165196

申请日：2021-02-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Todd ROSWECH ,  Jonathan SCHULTZ ,  Chun HO

IPC: B67D7/02 ,  B67D7/76 ,  B01L3/00

Abstract: A fluidic interconnect includes a first interface including a liquid port, a gas port, and a cradle; a second interface including a liquid port, a gas port, and a swing bar to engage the cradle, a weight of a container attached to one of the first or second interfaces to drive the liquid port of the first interface into connection with the liquid port of the second interface and the gas port of the first interface into connection with the gas port of the second interface.

公开(公告)号：US20190106745A1

公开(公告)日：2019-04-11

申请号：US16153898

申请日：2018-10-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Todd Rearick ,  Mark MILGREW ,  Jonathan SCHULTZ ,  Chris PAPALIAS ,  Kim JOHNSON

IPC: C12Q1/6874 ,  G06F19/22

Abstract: In one implementation, a method is described. The method includes determining an operational characteristic of sensors of a sensor array. The method further includes selecting a group of sensors in the array based on the operational characteristic of sensors in the group. The method further includes enabling readout of the sensors in the selected group. The method further includes receiving output signals from the enabled sensors, the output signals indicating chemical reactions occurring proximate to the sensors of the sensor array.

公开(公告)号：US20190032797A1

公开(公告)日：2019-01-31

申请号：US16148992

申请日：2018-10-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan SCHULTZ

IPC: F16K7/07 ,  F16K7/04 ,  G05D16/18 ,  G05D16/06 ,  B01L3/00 ,  C12Q1/6869

CPC classification number: F16K7/07 ,  B01L3/567 ,  B01L2200/06 ,  C12Q1/6869 ,  F16K7/04 ,  G05D16/0638 ,  G05D16/185

Abstract: A valve for regulating fluid flow includes a housing base defining a lower cavity and comprising a pinch structure within the lower cavity, a gas inlet providing external access to the lower cavity, a base fluid inlet, and a base fluid outlet. A housing cover defines an upper cavity and comprises a cover fluid inlet and a cover fluid outlet. The cover fluid inlet is in fluidic communication with the base fluid outlet between the upper cavity and the lower cavity, and the cover fluid outlet provides external access from the upper cavity. A diaphragm is disposed between the housing base and the housing cover. A pinch plate is disposed in the lower cavity and comprises a pinch point disposed opposite the pinch structure. A pinch tube is in fluidic communication between the base fluid inlet and the base fluid outlet in the lower cavity.

公开(公告)号：US20170121766A1

公开(公告)日：2017-05-04

申请号：US15404735

申请日：2017-01-12

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan SCHULTZ ,  John DAVIDSON

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  B01L3/5027 ,  C12Q1/6874 ,  C12Q2537/155 ,  C12Q2563/149 ,  C12Q2565/301 ,  C12Q2565/629

Abstract: A method of sequencing a polynucleotide strand can include providing the polynucleotide strand with a primer annealed thereto and a polymerase operably bound to the polynucleotide strand; successively exposing the polynucleotide strand to the flow of four different dNTPs according to a first predetermined ordering; and successively exposing the polynucleotide strand to the flow of four different dNTPs according to a second predetermined ordering, wherein the second predetermined ordering is different from the first predetermined ordering.

公开(公告)号：US20160168635A1

公开(公告)日：2016-06-16

申请号：US15051084

申请日：2016-02-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan M. ROTHBERG ,  James BUSTILLO ,  Mark James MILGREW ,  Jonathan SCHULTZ ,  David MARRAN ,  Todd REARICK ,  Kim L. JOHNSON

IPC: C12Q1/68 ,  G01N27/414

CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/4148 ,  H01L21/82 ,  C12Q2565/607

Abstract: The invention is directed to apparatus and chips comprising a large scale chemical field effect transistor arrays that include an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid for analysis. In one aspect such transistor arrays have a pitch of 10 μm or less and each sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region. In one embodiment, the characteristic of said chemical or biological sample is a concentration of a charged species and wherein each of said chemical field effect transistors is an ion-sensitive field effect transistor having a floating gate with a dielectric layer on a surface thereof, the dielectric layer contacting said sample fluid and being capable of accumulating charge in proportion to a concentration of the charged species in said sample fluid. In one embodiment such charged species is a hydrogen ion such that the sensors measure changes in pH of the sample fluid in or adjacent to the sample-retaining region thereof. Apparatus and chips of the invention may be adapted for large scale pH-based DNA sequencing and other bioscience and biomedical applications.

公开(公告)号：US20160010149A1

公开(公告)日：2016-01-14

申请号：US14862930

申请日：2015-09-23

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan M. ROTHBERG ,  James BUSTILLO ,  Mark James MILGREW ,  Jonathan SCHULTZ ,  David MARRAN ,  Todd REARICK ,  Kim L. JOHNSON

IPC: C12Q1/68 ,  G01N27/414

CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/4148 ,  H01L21/82 ,  C12Q2565/607

Abstract: An apparatus comprising a chemical field effect transistor array in a circuit-supporting substrate is disclosed. The transistor array has disposed on its surface an array of sample-retaining regions capable of retaining a chemical or biological sample from a sample fluid. The transistor array has a pitch of 10 μm or less and a sample-retaining region is positioned on at least one chemical field effect transistor which is configured to generate at least one output signal related to a characteristic of a chemical or biological sample in such sample-retaining region.

公开(公告)号：US20150369828A1

公开(公告)日：2015-12-24

申请号：US14741084

申请日：2015-06-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James A. BALL ,  Jonathan SCHULTZ

IPC: G01N33/84 ,  C12Q1/68

CPC classification number: G01N33/84 ,  B01J19/0086 ,  C12Q1/6869 ,  C12Q2527/119 ,  C12Q2527/125 ,  C12Q2565/607

Abstract: A buffered suspension includes a surfactant and a solid buffer particulate having a point of zero charge at least 1.2 pH units different that the pH of the buffered suspension. The buffered suspension can be prepared by mixing a stock solution with the solid buffer particulate and titrating. A method of preforming a pH sensitive process includes drawing the buffered suspension from a reservoir, filtering the solid buffer particulate from the buffered suspension, and applying the filtered solution to a sensor.

Abstract translation: 缓冲悬浮液包括表面活性剂和具有零电荷点的固体缓冲颗粒，其pH值不小于缓冲悬浮液的pH至少1.2pH单位。 缓冲悬浮液可以通过将储备溶液与固体缓冲颗粒混合并滴定来制备。 预处理pH敏感过程的方法包括从储存器中抽出缓冲的悬浮液，从缓冲的悬浮液中过滤固体缓冲颗粒，以及将过滤的溶液施加到传感器。