公开(公告)号：US20120102596A1

公开(公告)日：2012-04-26

申请号：US13299763

申请日：2011-11-18

申请人： William B. Allen ,  Bo Shen ,  Mitchell C. Tarczynski ,  Mark E. Williams ,  Peizhong Zheng ,  Gan-Yuan Zhong

发明人： William B. Allen ,  Bo Shen ,  Mitchell C. Tarczynski ,  Mark E. Williams ,  Peizhong Zheng ,  Gan-Yuan Zhong

IPC分类号： C12N15/82 ,  C12Q1/68 ,  C12N15/63 ,  A01H5/00 ,  C12N9/10 ,  C12N15/54

CPC分类号： C12N15/8247 ,  C12N9/1029 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12Q2600/172

摘要： Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods for their use are provided. The compositions are novel molecular marker loci genetically linked with QTL6 and which are associated with increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio of a plant. These novel markers are characterized by the presence of at least one polymorphism relative to the corresponding marker locus from the QTL6 region of non-high-oil, non-high-oleic acid maize plants. In some embodiments, the novel marker loci comprise coding sequence for a maize DGAT1-2 polypeptide or biologically active variant thereof. The marker loci of the invention, and suitable fragments thereof, are useful in methods for manipulating oil and/or oleic acid content and/or oleic acid/linoleic acid ratio of a plant, for marker-assisted selection of a plant, and for marker-assisted breeding of the high oil and/or high oleic acid trait.

摘要翻译： 提供了与玉米数量性状基因座6（QTL6）相关的成分及其使用方法。 所述组合物是与QTL6遗传连锁的新型分子标记基因座，其与植物的油含量增加和/或油酸含量增加和/或油酸/亚油酸比例增加相关。 这些新型标记物的特征在于相对于非高油非高油酸玉米植物的QTL6区域的相应标记基因座存在至少一个多态性。 在一些实施方案中，新的标记基因座包含玉米DGAT1-2多肽或其生物活性变体的编码序列。 本发明的标记基因座及其合适的片段可用于操作植物的油和/或油酸含量和/或油酸/亚油酸比例，用于植物的标记辅助选择和标记物的方法 - 高油和/或高油酸性状的辅助育种。

公开(公告)号：US20110264010A1

公开(公告)日：2011-10-27

申请号：US13177144

申请日：2011-07-06

申请人： Mark E. Williams

发明人： Mark E. Williams

IPC分类号： A61B5/11

CPC分类号： A61B5/112 ,  A61B5/1038 ,  A61B5/1123 ,  A61B5/1124 ,  A61B5/165 ,  A61B5/4076 ,  A61B5/4082 ,  A61B5/4519 ,  A61B5/4528 ,  A61B5/7257 ,  A61B5/7275 ,  G06F19/00 ,  G06K9/00348

摘要： According to some exemplary embodiments, a method or system can involve associating a plurality of biokinetographic comparison results with a first specific dysfunction from a group of specific dysfunctions, each of the biokinetographic comparison results obtained from a comparison of a biokinetographic value to a standard for a corresponding biokinetographic variable.

公开(公告)号：US20110041207A1

公开(公告)日：2011-02-17

申请号：US12875574

申请日：2010-09-03

申请人： William B. Allen ,  Bo Shen ,  Mitchell C. Tarczynski ,  Mark E. Williams ,  Peizhong Zheng ,  Gan-Yuan Zhong

发明人： William B. Allen ,  Bo Shen ,  Mitchell C. Tarczynski ,  Mark E. Williams ,  Peizhong Zheng ,  Gan-Yuan Zhong

IPC分类号： A01H1/02 ,  C12Q1/68 ,  A01H5/00 ,  A01H1/06

CPC分类号： C12N15/8247 ,  C12N9/1029 ,  C12Q1/6895 ,  C12Q2600/13 ,  C12Q2600/156 ,  C12Q2600/172

摘要： Compositions related to the quantitative trait locus 6 (QTL6) in maize and methods for their use are provided. The compositions are novel molecular marker loci that are genetically linked with QTL6 and which are associated with increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio of a plant or plant part thereof. These novel markers are characterized by the presence of at least one polymorphism relative to the corresponding marker locus from the QTL6 region of non-high-oil, non-high-oleic acid maize plants. In some embodiments, the novel marker loci comprise coding sequence for a maize DGAT1-2 polypeptide or biologically active variant thereof. The marker loci of the invention, and suitable fragments thereof, are useful in methods of the invention for manipulating oil and/or oleic acid content and/or oleic acid/linoleic acid ratio of a plant or plant part thereof, for marker-assisted selection of a plant, for example, a maize plant, or plant part thereof, having an increased oil content and/or increased oleic acid content and/or an increased oleic acid/linoleic acid ratio, and for marker-assisted breeding of the high oil and/or high oleic acid trait.

摘要翻译： 提供了与玉米数量性状基因座6（QTL6）相关的成分及其使用方法。 所述组合物是与QTL6遗传连锁的新型分子标记基因座，其与植物或植物部分的油含量增加和/或油酸含量增加和/或油酸/亚油酸含量增加相关。 这些新型标记物的特征在于相对于非高油非高油酸玉米植物的QTL6区域的相应标记基因座存在至少一个多态性。 在一些实施方案中，新的标记基因座包含玉米DGAT1-2多肽或其生物活性变体的编码序列。 本发明的标记基因座及其合适的片段可用于本发明的用于操纵植物或植物部分的油和/或油酸含量和/或油酸/亚油酸比例的方法，用于标记辅助选择 具有增加的油含量和/或油酸含量增加和/或油酸/亚油酸比例增加的植物，例如玉米植物或其植物部分，以及高油的标记辅助育种 和/或高油酸性状。

公开(公告)号：US07838263B2

公开(公告)日：2010-11-23

申请号：US11964871

申请日：2007-12-27

申请人： Thao Dam ,  Anthony D. Guida, Jr. ,  Christine B. Hazel ,  Bailin Li ,  Mark E. Williams

发明人： Thao Dam ,  Anthony D. Guida, Jr. ,  Christine B. Hazel ,  Bailin Li ,  Mark E. Williams

IPC分类号： C12Q1/26 ,  C12Q1/68

CPC分类号： C12N15/8278 ,  C12N15/8274

摘要： This invention relates to polynucleotide sequences encoding a gene that can confer resistance to at least one herbicide. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to at least one herbicide, and methods of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently select the best lines for breeding strategies by avoiding sensitive lines. Methods of identifying a plant with an Nsf1 locus using molecular markers are described.

摘要翻译： 本发明涉及编码可赋予至少一种除草剂抗性的基因的多核苷酸序列。 它进一步涉及携带包含所述多核苷酸序列的嵌合基因的植物和种子，其增强或赋予至少一种除草剂的抗性，以及制备所述植物和种子的方法。 本发明还提供可用作分子标记的序列，其又可用于鉴定由新杂交产生的玉米品系中的感兴趣区域，并通过避免敏感品系快速有效地选择育种策略的最佳品系。 描述了使用分子标记鉴定具有Nsf1基因座的植物的方法。

公开(公告)号：US07705200B2

公开(公告)日：2010-04-27

申请号：US12428648

申请日：2009-04-23

申请人： Thao Dam ,  Anthony D. Guida, Jr. ,  Christine B. Hazel ,  Bailin Li ,  Mark E. Williams

发明人： Thao Dam ,  Anthony D. Guida, Jr. ,  Christine B. Hazel ,  Bailin Li ,  Mark E. Williams

IPC分类号： C12N15/82 ,  A01H5/00 ,  A01N25/00

CPC分类号： C12N15/8278 ,  C12N15/8274

摘要： This invention relates to polynucleotide sequences encoding a gene that can confer resistance to at least one herbicide. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to at least one herbicide, and methods of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently select the best lines for breeding strategies by avoiding sensitive lines.

摘要翻译： 本发明涉及编码可赋予至少一种除草剂抗性的基因的多核苷酸序列。 它进一步涉及携带包含所述多核苷酸序列的嵌合基因的植物和种子，其增强或赋予至少一种除草剂的抗性，以及制备所述植物和种子的方法。 本发明还提供可用作分子标记的序列，其又可用于鉴定由新杂交产生的玉米品系中的感兴趣区域，并通过避免敏感品系快速有效地选择育种策略的最佳品系。

公开(公告)号：US07484572B2

公开(公告)日：2009-02-03

申请号：US11677151

申请日：2007-02-21

申请人： Mark P. Blackman ,  Mark E. Williams ,  William C. Saxman ,  Michael B. Crawford ,  Gerald L. Pruitt ,  William W. King ,  Bruce A. Rohde

发明人： Mark P. Blackman ,  Mark E. Williams ,  William C. Saxman ,  Michael B. Crawford ,  Gerald L. Pruitt ,  William W. King ,  Bruce A. Rohde

IPC分类号： E21B10/22

CPC分类号： E21B10/25 ,  E21B10/18 ,  E21B10/24

摘要： A roller cone drill bit having a bit body with at least one support arm extending therefrom. Each support arm may have an interior surface and an exterior surface with an associated spindle extending inwardly from the interior surface. A respective cone assembly may be rotatably disposed on each spindle. A gap may be formed between interior portions of each cone assembly and exterior portions of the associated spindle with a fluid seal disposed in the gap to block fluid flow therethrough. Each cone assembly may include a generally circular backface disposed adjacent to the interior surface of the associated support arm. At least one fluid passageway may extend through each support arm to allow communication of fluid from the interior surface to an exterior surface of the support arm. A debris diverter insert having a generally hollow bore may be disposed within each fluid passageway.

摘要翻译： 一种辊锥钻头，其具有从其延伸的至少一个支撑臂的钻头体。 每个支撑臂可以具有内表面和外表面，其中相关的心轴从内表面向内延伸。 相应的锥形组件可以可旋转地设置在每个心轴上。 可以在每个锥形组件的内部部分和相关主轴的外部部分之间形成间隙，其中设置在间隙中的流体密封件以阻止流过其中的流体流动。 每个锥形组件可以包括邻近相关支撑臂的内表面设置的大致圆形背面。 至少一个流体通道可以延伸穿过每个支撑臂，以允许流体从内表面连通到支撑臂的外表面。 具有大致中空孔的碎屑转向器插入件可设置在每个流体通道内。

公开(公告)号：US20080248040A1

公开(公告)日：2008-10-09

申请号：US10573764

申请日：2004-09-28

申请人： Menghang Xia ,  Mark E. Williams

发明人： Menghang Xia ,  Mark E. Williams

IPC分类号： A61K39/395 ,  C12N15/11 ,  C07K14/00 ,  C12N15/00 ,  A61K31/70 ,  G01N33/569 ,  C12P21/04 ,  A61P43/00 ,  C12Q1/68 ,  C07K16/18 ,  C12Q1/02 ,  C12N5/06 ,  G01N33/00 ,  G01N33/53

CPC分类号： C07K14/705

摘要： Disclosed are mammalian nucleic acid sequences encoding α1I subunit isoforms of a voltage-gated calcium channel. Specifically disclosed are novel variants of the α1I subunit designated herein as α1I-1 and α1I-2. In other aspects, the disclosure relates to expression vectors which encode the novel subunits of the invention, as well as cells containing such vectors. Antibodies specific for each of the variant subunits are also provided. The nucleic acid sequences of the invention find application, for example, in screening for compounds which modulate the activity of voltage-gated calcium channels and also in diagnostic methods for diagnosing various T-type channel mediated disorders, e.g., epilepsy, cancer, pain, sleep disorders and the autoimmune disease Lambert-Eaton Syndrome. Diagnosing defects in α1I subunit genes of a patient with a neuronal disease such as epilepsy are also included. An additional application of the nucleic acid sequences of the invention is in therapeutic methods of treatment for α1I subunit mediated disorders.

摘要翻译： 公开了编码电压门控钙通道的α1I亚基同种型的哺乳动物核酸序列。 具体公开的是本文称为α1I-1和α1I-2的α1I亚基的新变体。 在其他方面，本公开涉及编码本发明的新亚基的表达载体以及含有这些载体的细胞。 还提供了针对每个变体亚单位特异性的抗体。 本发明的核酸序列可用于例如筛选调节电压门控钙通道活性的化合物，以及用于诊断各种T型通道介导的病症的诊断方法，例如癫痫，癌症，疼痛， 睡眠障碍和自身免疫疾病兰伯特 - 伊顿综合征。 还包括诊断患有神经元疾病如癫痫患者的α1I亚基基因缺陷。 本发明的核酸序列的另外应用是治疗α1I亚基介导的病症的治疗方法。

公开(公告)号：US07370601B1

公开(公告)日：2008-05-13

申请号：US11283976

申请日：2005-11-21

申请人： Mark E. Williams

发明人： Mark E. Williams

IPC分类号： A01M29/02

CPC分类号： A01M29/18 ,  A01M29/16

摘要： Devices that emit sound audible to nearby animals can include a deflection structure or other support structure and, connected to it, at least one whistle structure. The deflection structure can, for example, be a rain guard, an insect deflector, or a rock deflector. It can, for example, have an air flow surface across which air flows when the vehicle on which it is mounted moves at normal operating speeds, and whistle structures can be activated by the air flow.

摘要翻译： 对附近动物发出声音的装置可以包括偏转结构或其他支撑结构，并且连接至少一个哨子结构。 偏转结构可以例如是防雨罩，昆虫导流板或摇摆板。 例如，当其上安装的车辆以正常操作速度移动时，它可以具有空气流动的空气流动表面，并且可以通过空气流激活口哨结构。

公开(公告)号：US07217862B2

公开(公告)日：2007-05-15

申请号：US11032490

申请日：2005-01-10

申请人： Rebecca E. Cahoon ,  Mark E. Williams

发明人： Rebecca E. Cahoon ,  Mark E. Williams

IPC分类号： A01H1/00 ,  C07H21/04 ,  C07H14/415 ,  C12N5/14 ,  C12N9/00

CPC分类号： C12N9/1085 ,  C12N15/825 ,  C12N2799/021 ,  C12Q1/6895 ,  C12Y205/01029

摘要： This invention relates to an isolated nucleic acid fragment encoding a geranylgeranyl pyrophosphate synthase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the geranylgeranyl pyrophosphate synthase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the geranylgeranyl pyrophosphate synthase in a transformed host cell.

摘要翻译： 本发明涉及编码香叶基香叶基焦磷酸合成酶的分离的核酸片段。 本发明还涉及在正义或反义方向上编码全部或一部分er牛儿基ger anyl基焦磷酸合酶的嵌合基因的构建，其中嵌合基因的表达导致在转化的宿主细胞中产生er牛儿基ger基焦磷酸合酶的改变水平 。

公开(公告)号：US06855868B2

公开(公告)日：2005-02-15

申请号：US10108915

申请日：2002-03-28

申请人： Rebecca E. Cahoon ,  Mark E. Williams

发明人： Rebecca E. Cahoon ,  Mark E. Williams

IPC分类号： C12N9/10 ,  C12N15/82 ,  A01H1/00 ,  C07H21/04 ,  C07K14/415 ,  C12N5/14 ,  C12N9/00

CPC分类号： C12N9/1085 ,  C12N15/825 ,  C12N2799/021 ,  C12Q1/6895 ,  C12Y205/01029

摘要： This invention relates to an isolated nucleic acid fragment encoding a geranylgeranyl pyrophosphate synthase. The invention also relates to the construction of a chimeric gene encoding all or a portion of the geranylgeranyl pyrophosphate synthase, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the geranylgeranyl pyrophosphate synthase in a transformed host cell.