公开(公告)号：US11535863B2

公开(公告)日：2022-12-27

申请号：US16439840

申请日：2019-06-13

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant G. Mali ,  Luhan Yang

IPC: C12N15/00 ,  C12N15/85 ,  C12N15/10 ,  C12N15/63 ,  C12N15/90 ,  C12N15/01 ,  C12N15/81 ,  C12N15/82 ,  C12N15/87 ,  C12N9/22

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US11359211B2

公开(公告)日：2022-06-14

申请号：US16397423

申请日：2019-04-29

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant G. Mali ,  Luhan Yang

IPC: C12N15/00 ,  C12N15/85 ,  C12N15/10 ,  C12N15/63 ,  C12N15/90 ,  C12N15/01 ,  C12N15/81 ,  C12N15/82 ,  C12N15/87 ,  C12N9/22

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US11236359B2

公开(公告)日：2022-02-01

申请号：US15042573

申请日：2016-02-12

Applicant: President and Fellows of Harvard College

Inventor： Prashant G. Mali ,  George M. Church ,  Luhan Yang

IPC: C12N15/85 ,  C12N15/10 ,  C12N15/63 ,  C12N15/90 ,  C12N15/01 ,  C12N15/81 ,  C12N15/82 ,  C12N15/87 ,  C12N9/22

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US20210100225A1

公开(公告)日：2021-04-08

申请号：US17118684

申请日：2020-12-11

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Luhan Yang ,  Marc Guell

IPC: A01K67/027 ,  C12N15/63 ,  C12N15/113 ,  C12N15/85 ,  C12N7/00 ,  C12N9/22 ,  C12N15/11 ,  C12N15/90

Abstract: A method of modulating some or all copies of a gene in a cell is provided including introducing into a cell one or more ribonucleic acid (RNA) sequences that comprise a portion that is complementary to all or a portion of each of the one or more target nucleic acid sequences, and a nucleic acid sequence that encodes a Cas protein and maintaining the cells under conditions in which the Cas protein is expressed and the Cas protein binds and modulates the one or more target nucleic acid sequences in the cell.

公开(公告)号：US20190352670A1

公开(公告)日：2019-11-21

申请号：US16482294

申请日：2018-01-31

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Luhan Yang

IPC: C12N15/90 ,  C12N9/22

Abstract: The present disclosure provides methods of improving nuclease-mediated gene targeting frequencies in cells using PFTα or bFGF.

公开(公告)号：US20180171361A1

公开(公告)日：2018-06-21

申请号：US15888581

申请日：2018-02-05

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Luhan Yang ,  Marc Guell

IPC: C12N15/90 ,  C12N15/11 ,  C12N9/22 ,  C12N7/00

CPC classification number: A01K67/0275 ,  A01K67/0273 ,  A01K67/0276 ,  A01K2207/05 ,  A01K2207/10 ,  A01K2227/108 ,  A01K2267/025 ,  C12N7/00 ,  C12N9/22 ,  C12N15/11 ,  C12N15/1132 ,  C12N15/63 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/907 ,  C12N2310/20 ,  C12N2740/10062 ,  C12N2800/80

Abstract: A method of modulating some or all copies of a gene in a cell is provided including introducing into a cell one or more ribonucleic acid (RNA) sequences that comprise a portion that is complementary to all or a portion of each of the one or more target nucleic acid sequences, and a nucleic acid sequence that encodes a Cas protein and maintaining the cells under conditions in which the Cas protein is expressed and the Cas protein binds and modulates the one or more target nucleic acid sequences in the cell.

公开(公告)号：US20150232833A1

公开(公告)日：2015-08-20

申请号：US14701912

申请日：2015-05-01

Applicant: PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Prashant G. Mali ,  George M. Church ,  Luhan Yang

IPC: C12N15/10

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US20140356958A1

公开(公告)日：2014-12-04

申请号：US14319100

申请日：2014-06-30

Applicant: President and Fellows of Harvard College

Inventor： Prashant G. MALI ,  George M. CHURCH ,  Luhan Yang

IPC: C12N15/85

CPC classification number: C12N15/85 ,  C12N9/22 ,  C12N15/01 ,  C12N15/10 ,  C12N15/102 ,  C12N15/1024 ,  C12N15/63 ,  C12N15/81 ,  C12N15/8201 ,  C12N15/87 ,  C12N15/90 ,  C12N15/907 ,  C12N2800/80 ,  C12N2810/55 ,  C12Y301/00

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US12018272B2

公开(公告)日：2024-06-25

申请号：US18296579

申请日：2023-04-06

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Prashant G. Mali ,  Luhan Yang

IPC: C12N15/63 ,  C12N9/22 ,  C12N15/01 ,  C12N15/10 ,  C12N15/81 ,  C12N15/82 ,  C12N15/85 ,  C12N15/87 ,  C12N15/90 ,  A61K48/00

CPC classification number: C12N15/85 ,  C12N9/22 ,  C12N15/01 ,  C12N15/10 ,  C12N15/102 ,  C12N15/1024 ,  C12N15/63 ,  C12N15/81 ,  C12N15/8201 ,  C12N15/87 ,  C12N15/90 ,  C12N15/907 ,  C12N2310/20 ,  C12N2800/80 ,  C12N2810/55 ,  C12Y301/00

Abstract: A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner.

公开(公告)号：US11746349B2

公开(公告)日：2023-09-05

申请号：US16076022

申请日：2017-02-07

Applicant: President and Fellows of Harvard College

Inventor： George M. Church ,  Luhan Yang ,  Margo R. Monroe ,  Po-Yi Huang

IPC: C12N15/113 ,  C12N9/22 ,  C12N9/96 ,  C12N1/15

CPC classification number: C12N15/113 ,  C12N9/22 ,  C12N9/96 ,  C12Y306/04013

Abstract: Methods and compositions of altering a eukaryotic cell are described including providing to the eukaryotic cell a guide DNA sequence complementary to a target nucleic acid sequence, providing to the eukaryotic cell an Ago enzyme or a nuclease null Ago protein that interacts with the guide DNA sequence for DNA-guided gene editing and regulation of the target nucleic acid sequence in a site specific manner.