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    Method of screening for protein inhibitors and activators
    35.
    发明授权
    Method of screening for protein inhibitors and activators 失效
    筛选蛋白质抑制剂和活化剂的方法

    公开(公告)号：US5877007A

    公开(公告)日：1999-03-02

    申请号：US473169

    申请日：1995-06-07

    申请人： Gerard Housey

    发明人： Gerard Housey

    IPC分类号： C12N1/21 ,  C12N9/12 ,  G01N33/50 ,  C12N1/00 ,  C12P21/02 ,  G01N33/573

    CPC分类号： C12N9/12 ,  G01N33/5008 ,  G01N33/5011 ,  G01N33/502 ,  G01N33/5026 ,  G01N2333/705 ,  G01N2333/82 ,  G01N2333/90

    摘要： Cells useful in the pharmacological characterization of inhibitors or activators of proteins are provided. These cells a protein, the presence of which affects a particular characteristic of the cell which changes in response to substances which inhibit or activate the protein. Such responsive changes in a phenotypic characteristic may be utilized in many useful ways, including the discovery, development or characterization of substances suitable for the treatment of diseases or other conditions in human beings or animals. Such cells may also be useful for studying diseases or other biological processes, for determining the effects of various drugs alone or in combination, as well as for identifying or characterizing substances which may be useful in reducing or preventing the occurrence of a disease or other condition. Through the use of such cells in a cell based assay, tamoxifen is identified as an inhibitor of Protein Kinase C (PKC) activity in cell culture.

    摘要翻译： 提供了可用于蛋白质抑制剂或激活剂的药理学表征的细胞。 这些细胞是蛋白质，其存在影响响应于抑制或激活蛋白质的物质而改变的细胞的特定特征。 表型特征的这种响应性变化可以以许多有用的方式使用，包括适用于治疗人或动物中的疾病或其他病症的物质的发现，开发或表征。 这样的细胞也可用于研究疾病或其他生物过程，用于确定单独或组合的各种药物的效果，以及用于鉴定或表征可用于减少或预防疾病或其他病症发生的物质 。 通过在基于细胞的测定中使用这样的细胞，将他莫昔芬鉴定为细胞培养物中蛋白激酶C（PKC）活性的抑制剂。

    Electrochemical Analyte Detection Apparatus and Method
    36.
    发明申请
    Electrochemical Analyte Detection Apparatus and Method 审中-公开

    公开(公告)号：US20180231552A1

    公开(公告)日：2018-08-16

    申请号：US15944148

    申请日：2018-04-03

    申请人： AgaMatrix, Inc.

    发明人： Ian Harding ,  Sridhar Iyengar ,  Richard Williams

    IPC分类号： G01N33/573 ,  C12Q1/00 ,  G01N33/543 ,  G01N27/416

    CPC分类号： G01N33/573 ,  C12Q1/001 ,  G01N27/4166 ,  G01N33/5438 ,  G01N2333/90 ,  G01N2333/902

    摘要： A method and apparatus for electrochemical detection of analyte in a sample makes use of a binding interaction and relies on the discovery that asymmetric distribution of a redox enzyme between two electrodes that occurs when a redox enzyme-containing reagent is immobilized at the surface of one electrode can be detected as a chemical potential gradient arising from an asymmetry in the distribution of oxidized or reduced redox substrate. This chemical potential gradient can be detected potentiometrically by observing the potential difference between the electrodes in an open circuit, or amperometrically by observing the current flow between the electrodes when the circuit is closed. In both cases, the observation of asymmetry can be done without the application of an external potential or current to the electrodes.

    Method for aiding diagnosis of Alzheimer's disease
    38.
    发明授权
    Method for aiding diagnosis of Alzheimer's disease 有权

    公开(公告)号：US09880178B2

    公开(公告)日：2018-01-30

    申请号：US14431557

    申请日：2013-10-03

    申请人： Randox Laboratories Ltd.

    发明人： Ellen Umlauf ,  Maria Zellner

    IPC分类号： G01N33/53 ,  G01N33/567 ,  G01N33/68 ,  G06F19/18 ,  G06F19/00

    CPC分类号： G01N33/6896 ,  G01N2333/4712 ,  G01N2333/775 ,  G01N2333/90 ,  G01N2333/90638 ,  G01N2333/91085 ,  G01N2800/2821 ,  G06F19/18 ,  G16H50/20

    摘要： The present invention provides an ex vivo method for aiding the diagnosis of Alzheimer's disease in a patient comprising: (i) determining the number of alleles of ApoE4 in the patient's genome; (ii) determining the combined expression level of at least three platelet proteins in a platelet sample from the patient; and (iii) comparing the resulting value of step (ii) to a control value, wherein the at least three platelet proteins include at least one isoform of alpha-tropomyosin containing exon 1a and at least two platelet proteins selected from monoamine oxidase-B, coagulation factor XIIIa, wild-type GSTO-1 or mutant GSTO-1, wherein a result higher than the control value is indicative of Alzheimer's disease.The invention also provides a solid support comprising one or more ligands of at least one isoform of alpha-tropomyosin containing exon 1a, and one or more ligands of at least two platelet proteins selected from monoamine oxidase-B, coagulation factor XIIIa, wild-type GSTO-1 protein and/or mutant GSTO-1 protein immobilized thereon.

    ANALYTICAL AND DIAGNOSTIC METHODS UTILIZING SHIGELLA FLEXNERI APYRASE
    39.
    发明申请
    ANALYTICAL AND DIAGNOSTIC METHODS UTILIZING SHIGELLA FLEXNERI APYRASE 审中-公开

    公开(公告)号：US20170321196A1

    公开(公告)日：2017-11-09

    申请号：US15524160

    申请日：2015-11-06

    申请人： ApiRays AB

    发明人： Pavankumar Asalapuram ,  Aman Russom

    IPC分类号： C12N9/14 ,  C12Q1/00 ,  C12Q1/34

    CPC分类号： C12N9/14 ,  C12Q1/008 ,  C12Q1/34 ,  G01N2333/90 ,  G01N2333/914

    摘要： A method, comprising the steps of providing a sample containing contaminating nucleoside diphosphates and/or nucleoside triphosphates, such as ATP and/or ATP analogues including deoxyribonucleoside triphosphates; reducing the amount of the contaminating nucleoside diphosphates and/or nucleoside triphosphates in the sample with an apyrase enzyme, wherein said apyrase enzyme is a Shigella flexneri apyrase; and performing an analysis of the sample, wherein said analysis comprises an assay that would have been affected by the contaminating nucleoside diphosphates and/or nucleoside triphosphates had they not been reduced in the reduction step.