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    RAPID DISCOVERY AND SCREENING OF ENZYME ACTIVITY USING MASS SPECTROMETRY
    2.
    发明申请
    RAPID DISCOVERY AND SCREENING OF ENZYME ACTIVITY USING MASS SPECTROMETRY 审中-公开
    使用大量光谱快速发现和筛选酶活性

    公开(公告)号：US20150330992A1

    公开(公告)日：2015-11-19

    申请号：US14651546

    申请日：2013-12-11

    申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  SANDIA CORPORATION

    发明人： Trent R Northen ,  Kai Deng ,  Tristan P De Rond ,  Pamela P Peralta-Yahya ,  Xiaoliang Cheng ,  Jay D Keasling

    IPC分类号： G01N33/68

    CPC分类号： G01N33/6848 ,  G01N2333/90

    摘要： Described herein are methods, systems, and compositions for detecting enzyme activity. In some embodiments, the reaction product(s) are coupled with a mass tag, and the enzyme activity is determiner by analyzing the reaction product(s). The enzyme assays can be performed using mass spectrometry, for example nanostructure-initiator mass spectrometry (NIMS). Also described are methods, systems, and compositions for monitoring enzymatic degradation process of a substrate sample, for example a biomass.

    摘要翻译： 本文描述了用于检测酶活性的方法，系统和组合物。 在一些实施方案中，将反应产物与质量标签偶联，通过分析反应产物来测定酶活性。 酶测定可以使用质谱法进行，例如纳米结构 - 引发剂质谱法（NIMS）。 还描述了用于监测底物样品例如生物质的酶降解过程的方法，系统和组合物。

    Electrochemical Analyte Detection Apparatus and Method
    4.
    发明申请
    Electrochemical Analyte Detection Apparatus and Method 审中-公开
    电化学分析物检测装置及方法

    公开(公告)号：US20090026075A1

    公开(公告)日：2009-01-29

    申请号：US12179446

    申请日：2008-07-24

    申请人： Ian Harding ,  Sridhar Iyengar ,  Richard Williams

    发明人： Ian Harding ,  Sridhar Iyengar ,  Richard Williams

    IPC分类号： G01N33/50

    CPC分类号： G01N33/573 ,  C12Q1/001 ,  G01N27/4166 ,  G01N33/5438 ,  G01N2333/90 ,  G01N2333/902

    摘要： A method and apparatus for electrochemical detection of analyte in a sample makes use of a binding interaction and relies on the discovery that asymmetric distribution of a redox enzyme between two electrodes that occurs when a redox enzyme-containing reagent is immobilized at the surface of one electrode can be detected as a chemical potential gradient arising from an asymmetry, in the distribution of oxidized or reduced redox substrate. This chemical potential gradient can be detected potentiometrically by observing the potential difference between the electrodes in an open circuit, or amperometrically by observing the current flow between the electrodes when the circuit is closed. In both cases, the observation of asymmetry can be done without the application of an external potential or current to the electrodes.

    摘要翻译： 用于样品中分析物的电化学检测的方法和装置利用结合相互作用，并且依赖于以下发现：当在含有氧化还原酶的试剂固定在一个电极的表面时发生的氧化还原酶在两个电极之间的不对称分布 可以被检测为由氧化还原氧化还原底物的分布中不对称引起的化学势梯度。 可以通过在断路中观察电极之间的电势差，或者当电路闭合时通过观察电极之间的电流来进行电流分析来电位检测该化学势梯度。 在这两种情况下，可以在不对电极施加外部电位或电流的情况下进行观察不对称。

    Fluorous peptide microarray, detection process using a fluorous peptide microarray, and process of forming a fluorous peptide microarray
    5.
    发明授权
    Fluorous peptide microarray, detection process using a fluorous peptide microarray, and process of forming a fluorous peptide microarray 有权

    公开(公告)号：US09977019B2

    公开(公告)日：2018-05-22

    申请号：US14394435

    申请日：2013-04-16

    申请人： MS2 ARRAY LLC

    发明人： Marvin Yu

    IPC分类号： G01N33/543 ,  G01N33/58

    CPC分类号： G01N33/54386 ,  G01N33/54306 ,  G01N33/582 ,  G01N2333/90

    摘要： A fluorous peptide microarray, a process of detecting information by using a fluorous peptide microarray, and a process of forming a fluorous peptide microarray are disclosed. The fluorous peptide microarray includes a covalently-modified conductive fluorous surface and fluorous-tagged peptides having natural amino acids positioned on the covalently-modified conductive fluorous surface. The fluorous-tagged peptides are configured for analysis of one or both of a protein and an enzyme. The process of detecting information includes using the fluorous peptide microarray. The process of forming the fluorous peptide microarray includes spotting the fluorous-modified composition to the fluorous surface after a solution phase reaction or transfer blotting the fluorous-modified composition.

    Electrochemical analyte detection apparatus and method
    6.
    发明授权
    Electrochemical analyte detection apparatus and method 有权

    公开(公告)号：US09939440B2

    公开(公告)日：2018-04-10

    申请号：US15017787

    申请日：2016-02-08

    申请人： AgaMatrix, Inc.

    发明人： Ian Harding ,  Sridhar Iyengar ,  Richard Williams

    IPC分类号： G01N27/416 ,  G01N33/573 ,  C12Q1/00 ,  G01N33/543

    CPC分类号： G01N33/573 ,  C12Q1/001 ,  G01N27/4166 ,  G01N33/5438 ,  G01N2333/90 ,  G01N2333/902

    摘要： A method and apparatus for electrochemical detection of analyte in a sample makes use of a binding interaction and relies on the discovery that asymmetric distribution of a redox enzyme between two electrodes that occurs when a redox enzyme-containing reagent is immobilized at the surface of one electrode can be detected as a chemical potential gradient arising from an asymmetry in the distribution of oxidized or reduced redox substrate. This chemical potential gradient can be detected potentiometrically by observing the potential difference between the electrodes in an open circuit, or amperometrically by observing the current flow between the electrodes when the circuit is closed. In both cases, the observation of asymmetry can be done without the application of an external potential or current to the electrodes.

    METHOD FOR AIDING DIAGNOSIS OF ALZHEIMER'S DISEASE
    8.
    发明申请
    METHOD FOR AIDING DIAGNOSIS OF ALZHEIMER'S DISEASE 有权
    帮助阿尔茨海默病诊断的方法

    公开(公告)号：US20150247870A1

    公开(公告)日：2015-09-03

    申请号：US14431557

    申请日：2013-10-03

    申请人： RANDOX LABORATORIES LTD

    发明人： Ellen Umlauf ,  Maria Zellner

    IPC分类号： G01N33/68 ,  G06F19/00 ,  G06F19/18

    CPC分类号： G01N33/6896 ,  G01N2333/4712 ,  G01N2333/775 ,  G01N2333/90 ,  G01N2333/90638 ,  G01N2333/91085 ,  G01N2800/2821 ,  G06F19/18 ,  G16H50/20

    摘要： The present invention provides an ex vivo method for aiding the diagnosis of Alzheimer's disease in a patient comprising: (i) determining the number of alleles of ApoE4 in the patient's genome; (ii) determining the combined expression level of at least three platelet proteins in a platelet sample from the patient; and (iii) comparing the resulting value of step (ii) to a control value, wherein the at least three platelet proteins include at least one isoform of alpha-tropomyosin containing exon 1a and at least two platelet proteins selected from monoamine oxidase-B, coagulation factor XIIIa, wild-type GSTO-1 or mutant GSTO-1, wherein a result higher than the control value is indicative of Alzheimer's disease.The invention also provides a solid support comprising one or more ligands of at least one isoform of alpha-tropomyosin containing exon 1a, and one or more ligands of at least two platelet proteins selected from monoamine oxidase-B, coagulation factor XIIIa, wild-type GSTO-1 protein and/or mutant GSTO-1 protein immobilised thereon.

    摘要翻译： 本发明提供一种用于辅助患者中阿尔茨海默病诊断的离体方法，包括：（i）确定患者基因组中ApoE4的等位基因数; （ii）确定来自患者的血小板样品中至少三种血小板蛋白的组合表达水平; 和（iii）将步骤（ii）的所得值与对照值进行比较，其中所述至少三种血小板蛋白包括至少一种含有外显子1a的α-原肌球蛋白的同种型和至少两种选自单胺氧化酶-B的血小板蛋白， 凝血因子XIIIa，野生型GSTO-1或突变型GSTO-1，其中高于对照值的结果指示阿尔茨海默病。 本发明还提供了一种固体支持物，其包含一种或多种至少一种含有α-原肌球蛋白的外显子1a同种型的配体，以及一种或多种选自单胺氧化酶-B，凝血因子XIIIa，野生型的至少两种血小板蛋白的配体 固定在其上的GSTO-1蛋白和/或突变型GSTO-1蛋白。