公开(公告)号：US20210349049A1

公开(公告)日：2021-11-11

申请号：US17443260

申请日：2021-07-23

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Michael Marquant ,  Samuel Evgin ,  Melanie Klein

IPC: G01N27/327

Abstract: A method for determining a concentration of at least one analyte in bodily fluid, comprising: a signal generation step, wherein an excitation voltage signal is generated by a signal generator, wherein the excitation voltage signal comprises a poly frequent alternating current (AC) voltage and a direct current (DC) voltage profile, wherein the poly frequent AC voltage comprises at least two frequencies; a signal application step, wherein the excitation voltage signal is applied to at least two measurement electrodes; a measurement step, wherein a response is measured by using the measurement electrodes; an evaluation step, wherein an AC current response for each frequency and a DC current response are evaluated from the response; a determination step, wherein the concentration of the analyte is determined from the DC current response and from one or both of the phase and impedance information by using at least one predetermined relationship.

公开(公告)号：US11169061B2

公开(公告)日：2021-11-09

申请号：US16542786

申请日：2019-08-16

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Claudio Cherubini ,  Martin Kopp ,  Nenad Milicevic ,  Daniel Mueller ,  Emad Sarofim ,  Goran Savatic

IPC: G01N1/40 ,  G01N15/04 ,  G01N35/00 ,  G01N35/10 ,  B01L3/00 ,  C12M1/00 ,  B04B5/04 ,  B04B7/08 ,  B04B11/04 ,  B04B13/00

Abstract: A method and system for processing particles contained in a liquid biological sample is presented. The method uses a rotatable vessel for processing particles contained in a liquid biological sample. The rotatable vessel has a longitudinal axis about which the vessel is rotatable, an upper portion having a top opening for receiving the liquid containing the particles, a lower portion for holding the liquid while the rotatable vessel is resting, the lower portion having a bottom, and an intermediate portion located between the upper portion and the lower portion, the intermediate portion having a lateral collection chamber for holding the liquid while the rotatable vessel is rotating. The method employs dedicated acceleration and deceleration profiles for sedimentation and re-suspension of the particles of interest.

公开(公告)号：US11150247B2

公开(公告)日：2021-10-19

申请号：US16204580

申请日：2018-11-29

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Ralf Bollhagen ,  Barbara Upmeier ,  Toralf Zarnt ,  Peter Muench ,  Manfred Ginter

IPC: G01N33/576 ,  G01N33/53

Abstract: The present disclosure relates to a method for detecting a core polypeptide of a hepatitis C virus (HCV) in a sample from a subject involving (a) contacting said sample with a base and with a surfactant having a cationic detergent, and (b) detecting a core polypeptide of the HCV in the sample. The present invention further relates to a method for pre-processing a sample from a subject for detection of HCV, involving contacting the sample with a base and with a surfactant having a cationic detergent; and to a pre-processing reagent for detecting HCV in a sample, having a base and a surfactant including a cationic detergent, wherein the surfactant also has a nonionic detergent. Moreover, the present disclosure further relates to kits, uses, and devices related to the methods disclosed.

公开(公告)号：US11112419B2

公开(公告)日：2021-09-07

申请号：US16031446

申请日：2018-07-10

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Hans van Mierlo ,  Gottlieb Schacher ,  Marcel Kaeppeli ,  Reto Huesser ,  Andreas Drechsler ,  Matthias Goetz ,  Nenad Milicevic

IPC: G01N35/02 ,  B65G17/00 ,  B65G17/12 ,  B65G17/46 ,  B65G47/57 ,  B65G17/32 ,  B65G17/34 ,  B65G43/00 ,  G01N35/04

Abstract: A laboratory distribution system is presented. The system comprises diagnostic laboratory container carriers and a conveyor. The conveyor comprises an endless drive defining a closed-loop conveyor pathway. The system comprises supporting elements attached to the endless drive. The supporting elements receive a container carrier and transport the container carrier in an upright position along a pathway section. The supporting elements are mounted pivotally about a horizontal pivot axis to the drive and structured such that a center of gravity of the supporting element with or without an empty or loaded container carrier is arranged below and vertically aligned with the pivot axis when the supporting element is in an upright position such that each supporting element is free to pivot about the associated pivot axis under the effect of gravitational forces acting on the supporting element for maintaining the supporting elements in an upright position while travelling along the path.

公开(公告)号：US11112365B2

公开(公告)日：2021-09-07

申请号：US15909295

申请日：2018-03-01

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Rik Harbers ,  Kurt Schildknecht

IPC: G01N21/75 ,  G01J3/28 ,  G01J3/42 ,  G01N21/25

Abstract: An optical device for determining the presence and/or concentration of analytes in a sample is presented. The optical device comprises a detector and a detection unit comprising optical path components. The detection unit has wavelength-dependent responsivity. The optical device further comprises a light source for emitting light of different respective usable wavelength ranges. The light is guidable through the optical path to the detector to generate baseline signals and response signals relative to the baseline signal indicative of the presence and/or concentration of analytes in the optical path. The intensity of the light reaching the detector is adjusted inverse to the wavelength-dependent responsivity with respect to at least two respective usable wavelength ranges so that a reduction of the ratio between the maximum baseline signal at one of the selected usable wavelength ranges and the minimum baseline signal at another of the selected usable wavelength ranges is obtained.

公开(公告)号：US20210270704A1

公开(公告)日：2021-09-02

申请号：US17303092

申请日：2021-05-19

Applicant: Ventana Medical Systems, Inc. ,  Roche Diagnostics Operations, Inc. ,  Roche Diagnostics Hematology, Inc.

Inventor： Srabani Bhaumik ,  Simon J. Davidson ,  Jerome W. Kosmeder, II ,  Katherine K. Mui ,  Esteban Roberts ,  Noemi Sebastiao ,  David J. Zahniser

IPC: G01N1/30 ,  G01N33/50

Abstract: This disclosure relates generally to the use of automated platforms in the preparation of biomarker-stained cellular samples for microscopic analysis and use of such stained cells in the diagnosis of certain conditions. Disclosed herein is a method of affinity staining a Romanowsky-type stained sample on automated advanced staining systems, wherein the automated advanced stainer destains the sample prior to contact with a biomarker-specific reagent. Also disclosed herein are methods of processing body fluid samples for morphological and biomarker analysis by depositing cells of the sample in a thin layer onto one or more solid supports, staining at least one such solid support with a Romanowsky-type stain and staining at least one such solid support for one or more biomarkers useful for categorizing one or more cells of the sample.

公开(公告)号：US11099180B2

公开(公告)日：2021-08-24

申请号：US16528710

申请日：2019-08-01

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Ralf Bollhagen ,  Barbara Upmeier ,  Werner Naser ,  Toralf Zarnt

IPC: G01N33/53 ,  G01N33/543 ,  G01N33/576

Abstract: The disclosure concerns a method and kits for measurement of an analyte in a microparticle-based analyte-specific binding assay. In the assay, the microparticles are coated with the first partner of a binding pair, mixing the coated microparticles and at least two analyte-specific binding agents, each conjugated to the second partner of the binding pair, and a sample suspected of containing the analyte. The second partner of the binding pair is bound to each of the analyte-specific binding agents via a linker comprising from 12 to 30 ethylene glycol units (PEG 12 to 30), thereby binding the analyte via the conjugated analyte-specific binding agents to the coated microparticles. The method also entails separating the microparticles having the analyte bound via the binding pair and the analyte-specific binding agent from the mixture and measuring the analyte bound to the microparticles.

公开(公告)号：US11078241B2

公开(公告)日：2021-08-03

申请号：US16751609

申请日：2020-01-24

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Barbara Upmeier ,  Ralf Bollhagen ,  Toralf Zarnt

IPC: C07K14/005 ,  G01N33/576

Abstract: The disclosure relates to a multi-epitope fusion protein as well as to its use as calibrator and/or control in an in vitro diagnostics immunoassay for detecting HCV core antigen. The multi-epitope fusion protein has two to six different non-overlapping linear peptides present in the amino acid sequence of hepatitis C virus (HCV) core protein, wherein each of the peptides is separated from the other peptides by a spacer consisting of a non-HCV amino acid sequence and having a chaperone amino acid sequence. No further HCV specific amino acid sequences are present in the polypeptide. A further aspect relates to a reagent kit for detecting HCV core antigen containing said multi-epitope fusion protein as calibrator or control or both.

公开(公告)号：US20210215731A1

公开(公告)日：2021-07-15

申请号：US17142359

申请日：2021-01-06

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Alejandro Morcillo Montejo ,  Marco Maetzler ,  Juan Bacardit

IPC: G01N35/10

Abstract: A computer-implemented method for processing biological samples in an environment including multiple automated analyzers having multiple modules is presented. The method includes receiving data related to a plurality of orders for processing biological samples at a main workflow engine. The main workflow engine receives and processes data for each of the multiple automated analyzers having multiple modules. The method further includes providing a data snapshot to at least one shadow workflow engine associated with a subset of one or more modules of the multiple modules. The data snapshot comprises only a portion of the data related to the plurality of orders that is necessary to determine actions of the module of the multiple modules. The method further includes determining, by the shadow workflow engine, a plurality of actions for the subset of modules to process the plurality of orders based on the data snapshot.

公开(公告)号：US20210213450A1

公开(公告)日：2021-07-15

申请号：US17141786

申请日：2021-01-05

Applicant: Roche Diagnostics Operations, Inc.

Inventor： Andreas Drechsler ,  Darko Klinec ,  Michael Koehler ,  Alexander Jakob ,  Michael Zeder

IPC: B01L3/00 ,  G01N23/20091

Abstract: A method to process a laboratory carrier in a laboratory system based on a feature of a test liquid in the laboratory carrier is presented. The laboratory system comprises the laboratory carrier comprising the test liquid, a terahertz wave source, a terahertz detector, a laboratory carrier processing device, and a control unit. Using terahertz technology and data analysis, the feature of the test liquid in the laboratory carrier can be determined. In addition, the control unit controls the laboratory carrier processing device based on the determined feature of the test liquid.