公开(公告)号：US20110269983A1

公开(公告)日：2011-11-03

申请号：US13044984

申请日：2011-03-10

Applicant: ANTHONY J. KINNEY ,  EDGAR BENJAMIN CAHOON ,  HOWARD GLENN DAMUDE ,  ZHAN-BIN LIU

Inventor： ANTHONY J. KINNEY ,  EDGAR BENJAMIN CAHOON ,  HOWARD GLENN DAMUDE ,  ZHAN-BIN LIU

IPC: C07C53/00 ,  A01H5/10 ,  A01H5/00

CPC classification number: C12N9/0083 ,  A21D2/165 ,  A23D9/00 ,  A23K20/158 ,  A23K50/40 ,  A23K50/80 ,  A23L11/01 ,  A23L11/03 ,  A23L11/05 ,  A23L11/07 ,  A23L33/12 ,  A23V2002/00 ,  A23V2300/21 ,  C12N9/1029 ,  C12N15/8247 ,  Y02A40/818 ,  A23V2250/1876 ,  A23V2250/1882

Abstract: Oilseed plants which have been transformed to produce very long chain polyunsaturated fatty acids, recombinant constructs used in such transformations, methods for producing such fatty acids in a plant are described and uses of oils and seeds obtained from such transformed plants in a variety of food and feed applications are described.

Abstract translation: 已经转化以产生非常长链多不饱和脂肪酸的油籽植物，用于这种转化的重组构建体，在植物中生产这种脂肪酸的方法，以及从这些转化植物获得的油和种子在多种食物中的应用，以及 描述饲料应用。

公开(公告)号：US07943823B2

公开(公告)日：2011-05-17

申请号：US11737772

申请日：2007-04-20

Applicant: Howard Glenn Damude ,  Quinn Qun Zhu

Inventor： Howard Glenn Damude ,  Quinn Qun Zhu

IPC: A01H5/00 ,  C12N15/82 ,  C12N5/10 ,  C07H21/04

CPC classification number: C12N15/8247 ,  C12N9/0083

Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding a delta-8 desaturase along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using this delta-8 desaturase in plants and oleaginous yeast are disclosed.

公开(公告)号：US07879591B2

公开(公告)日：2011-02-01

申请号：US12485141

申请日：2009-06-16

Applicant: Howard Glenn Damude ,  Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

Inventor： Howard Glenn Damude ,  Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

IPC: C12N1/16 ,  C12N9/10 ,  C12N15/70 ,  C12P21/06 ,  C07H21/04

CPC classification number: C12P7/6472 ,  A23K20/158 ,  A23L33/12 ,  A23V2002/00 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/52 ,  C12P7/6427 ,  A23V2250/187 ,  A23V2200/30

Abstract: Lysophosphatidic acid acyltransferase [“LPAAT”] participates in the second step of oil biosynthesis and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids [“LC-PUFAs”] produced in oils of oleaginous organisms. An LPAAT isolated from Mortierella alpina [“MaLPAAT1”] that is suitable for use in the manufacture of oils enriched in LC-PUFAs in oleaginous organisms is disclosed. Most desirably, the substrate specificity of the instant MaLPAAT1 will be particularly useful to enable increased C18 to C20 elongation conversion efficiency and increased Δ4 desaturation conversion efficiency in recombinant host cells producing LC-PUFAs.

Abstract translation: 溶血磷脂酰基转移酶[“LPAAT”]参与了油生物合成的第二步，预计在改变含油生物油中产生的长链多不饱和脂肪酸（“LC-PUFA”）的量方面起关键作用。 公开了一种适用于制造含油生物中富含LC-PUFA的油的高山被孢霉[“MaLPAAT1”]分离的LPAAT。 最令人满意的是，本发明的MaLPAAT1的底物特异性将特别有用，以便能够在产生LC-PUFA的重组宿主细胞中提高C18至C20延伸转换效率和增加和降低转化效率。

公开(公告)号：US20100175148A1

公开(公告)日：2010-07-08

申请号：US12725482

申请日：2010-03-17

Applicant: Howard Glenn Damude ,  Brian McGonigle ,  Quinn Qun Zhu ,  Zhixiong Xue

Inventor： Howard Glenn Damude ,  Brian McGonigle ,  Quinn Qun Zhu ,  Zhixiong Xue

IPC: C12P7/64 ,  C07H21/04 ,  C12N9/02 ,  C12N15/82 ,  C12N5/10 ,  C12N15/63 ,  A01H1/00 ,  A01H5/10 ,  A01H5/00

CPC classification number: C12N15/8247 ,  C11B1/025 ,  C12N9/1029 ,  C12P7/6427 ,  C12P7/6463 ,  C12P7/6472

Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding delta-9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these delta-9 elongases in plants.

Abstract translation: 分离的核酸片段和包含编码Δ-9延伸酶的这种片段的重组构建物以及在植物中使用这些δ-9延长酶制备长链多不饱和脂肪酸（PUFA）的方法。

公开(公告)号：US20100075387A1

公开(公告)日：2010-03-25

申请号：US12619706

申请日：2009-11-17

Applicant: HOWARD GLENN DAMUDE ,  BRIAN MCGONIGLE ,  ZHIXIONG XUE ,  QUINN QUN ZHU

Inventor： HOWARD GLENN DAMUDE ,  BRIAN MCGONIGLE ,  ZHIXIONG XUE ,  QUINN QUN ZHU

IPC: C12P7/64 ,  C07H21/02 ,  C12N9/10 ,  C12N1/21 ,  C12N1/13 ,  C12N1/19 ,  C07D303/12

CPC classification number: C12N15/8247 ,  C11B1/025 ,  C12N9/1029 ,  C12P7/6427 ,  C12P7/6463 ,  C12P7/6472

Abstract: The present invention relates to Δ9 elongases, which have the ability to convert linoleic acid [18:2, LA] to eicosadienoic acid [20:2, EDA]. Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ9 elongase along with methods of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ9 elongases in plants and oleaginous yeast are disclosed.

Abstract translation: 本发明涉及具有将亚油酸[18：2，LA]转化成二十碳烯酸[20：2，EDA]的能力的“Dgr”9延伸酶。 公开了分离的核酸片段和重组构建体，其包含编码'Dgr 9延长酶的这种片段以及使用这些植物和油性酵母中的这些Dgr 9延伸酶制备长链多不饱和脂肪酸（PUFA）的方法。

公开(公告)号：US20090325265A1

公开(公告)日：2009-12-31

申请号：US12485141

申请日：2009-06-16

Applicant: Howard Glenn Damude ,  Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

Inventor： Howard Glenn Damude ,  Zhixiong Xue ,  Narendra S. Yadav ,  Quinn Qun Zhu

IPC: C12N9/10 ,  C12N1/19 ,  C12N15/63 ,  C07H21/04

CPC classification number: C12P7/6472 ,  A23K20/158 ,  A23L33/12 ,  A23V2002/00 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/52 ,  C12P7/6427 ,  A23V2250/187 ,  A23V2200/30

Abstract: Lysophosphatidic acid acyltransferase [“LPAAT”] participates in the second step of oil biosynthesis and is expected to play a key role in altering the quantity of long-chain polyunsaturated fatty acids [“LC-PUFAs”] produced in oils of oleaginous organisms. An LPAAT isolated from Mortierella alpina [“MaLPAAT1”] that is suitable for use in the manufacture of oils enriched in LC-PUFAs in oleaginous organisms is disclosed. Most desirably, the substrate specificity of the instant MaLPAAT1 will be particularly useful to enable increased C18 to C20 elongation conversion efficiency and increased Δ4 desaturation conversion efficiency in recombinant host cells producing LC-PUFAs.

Abstract translation: 溶血磷脂酰基转移酶[“LPAAT”]参与了油生物合成的第二步，预计在改变含油生物油中产生的长链多不饱和脂肪酸（“LC-PUFA”）的量方面起关键作用。 公开了一种适用于制造含油生物中富含LC-PUFA的油的高山被孢霉[“MaLPAAT1”]分离的LPAAT。 最令人满意的是，本发明的MaLPAAT1的底物特异性将特别有用，以便在产生LC-PUFA的重组宿主细胞中提高C18至C20延伸转换效率和增加Delta4去饱和转化效率。

公开(公告)号：US20090320161A1

公开(公告)日：2009-12-24

申请号：US12487768

申请日：2009-06-19

Applicant: Brian McGonigle ,  Anthony J. Kinney ,  Howard Glenn Damude

Inventor： Brian McGonigle ,  Anthony J. Kinney ,  Howard Glenn Damude

IPC: C12N15/82 ,  C12N15/11 ,  C12N15/00 ,  C12N5/04 ,  C07C57/00 ,  A01H5/00 ,  C12P7/64 ,  C12Q1/68

CPC classification number: C12N15/8247 ,  A23D9/00 ,  C12N9/1029 ,  C12P7/6427

Abstract: Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding novel delta-9 elongases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these delta-9 elongases in plants.

Abstract translation: 分离的核酸片段和包含编码新的Δ-9延伸酶的这种片段的重组构建物以及在植物中使用这些δ-9延长酶制备长链多不饱和脂肪酸（PUFA）的方法。

公开(公告)号：US20090311380A1

公开(公告)日：2009-12-17

申请号：US12466072

申请日：2009-05-14

Applicant: Howard Glenn Damude ,  Peter John Gillies ,  Daniel Joseph Macool ,  Stephen K. Picataggio ,  James John Ragghianti ,  John E. Seip ,  Zhixiong Xue ,  Narendra S. Yadav ,  Hongxiang Zhang ,  Quinn Qun Zhu

Inventor： Howard Glenn Damude ,  Peter John Gillies ,  Daniel Joseph Macool ,  Stephen K. Picataggio ,  James John Ragghianti ,  John E. Seip ,  Zhixiong Xue ,  Narendra S. Yadav ,  Hongxiang Zhang ,  Quinn Qun Zhu

IPC: A23L1/30 ,  C12N1/19 ,  C07C57/03 ,  A23L1/28 ,  A23C9/12 ,  A23K1/16

CPC classification number: C12P7/6427 ,  A23D9/00 ,  A23K20/158 ,  A23L33/12 ,  A23V2002/00 ,  C12N1/16 ,  C12N9/0083 ,  C12N9/1029 ,  C12N15/52 ,  C12N15/746 ,  C12N15/815 ,  C12P7/6472 ,  A23V2250/187 ,  A23V2200/30

Abstract: An engineered strain of the oleaginous yeast Yarrowia lipolytica capable of producing greater than 5.6% docosahexaenoic acid acid (DHA, an w-3 polyunsaturated fatty acid) in the total oil fraction is described. This strain comprises various chimeric genes expressing heterologous desaturases, elongases and acyltransferases and optionally comprises various native desaturase and acyltransferase knockouts to enable synthesis and high accumulation of DHA. Production host cells are claimed, as are methods for producing DHA within said host cells.

Abstract translation: 描述了能够在总油馏分中产生大于5.6％二十二碳六烯酸（DHA，w-3多不饱和脂肪酸）的含油酵母解脂耶氏酵母解脂耶氏酵母的改造菌株。 该菌株包含表达异源去饱和酶，延长酶和酰基转移酶的各种嵌合基因，并且任选地包含各种天然去饱和酶和酰基转移酶敲除，以使得能够合成和高浓度的DHA。 要求生产宿主细胞，以及在所述宿主细胞内产生DHA的方法。

公开(公告)号：US20090274816A1

公开(公告)日：2009-11-05

申请号：US12420345

申请日：2009-04-08

Applicant: Howard Glenn Damude ,  Narendra S. Yadav

Inventor： Howard Glenn Damude ,  Narendra S. Yadav

IPC: A23K1/16 ,  C12N15/74 ,  A01H5/00 ,  C11B1/00 ,  A01H1/00

CPC classification number: C12N9/0083 ,  C12N15/8247 ,  C12P7/6427 ,  C12P7/6472

Abstract: The present invention relates to fungal Δ-15 fatty acid desaturases that are able to catalyze the conversion of linoleic acid (18:2, LA) to alpha-linolenic acid (18:3, ALA). Nucleic acid sequences encoding the desaturases, nucleic acid sequences which hybridize thereto, DNA constructs comprising the desaturase genes, and recombinant host plants and microorganisms expressing increased levels of the desaturases are described. Methods of increasing production of specific omega-3 and omega-6 fatty acids by over-expression of the Δ-15 fatty acid desaturases are also described herein.

Abstract translation: 本发明涉及能够催化亚油酸（18:2，LA）转化为α-亚麻酸（18:3，ALA）的真菌Δ-15脂肪酸去饱和酶。 描述了编码去饱和酶的核酸序列，与其杂交的核酸序列，包含去饱和酶基因的DNA构建体和表达增加的去饱和酶水平的重组宿主植物和微生物。 本文还描述了通过过表达Δ-15脂肪酸去饱和酶增加特定ω-3和ω-6脂肪酸的产生的方法。

公开(公告)号：US20080274521A1

公开(公告)日：2008-11-06

申请号：US12111237

申请日：2008-04-29

Applicant: Howard Glenn Damude ,  Quinn Qun Zhu

Inventor： Howard Glenn Damude ,  Quinn Qun Zhu

IPC: C12P7/64 ,  C12N1/21 ,  C12N1/15 ,  C12N1/19 ,  C12N15/52 ,  C12N1/13 ,  C12N1/11

CPC classification number: C12N9/0071 ,  A23D9/00 ,  A23D9/02 ,  A23K20/158 ,  A23L33/115 ,  C12N9/0083 ,  C12N15/8247 ,  C12Y114/19

Abstract: The present invention relates to Δ5 desaturases, which have the ability to convert dihomo-γ-linolenic acid (DGLA; 20:3 ω-6) to arachidonic acid (ARA; 20:4 ω-6) and/or eicosatetraenoic acid (ETA; 20:4 ω-3) to eicosapentaenoic acid (EPA; 20:5 ω-3). Isolated nucleic acid fragments and recombinant constructs comprising such fragments encoding Δ5 desaturases along with a method of making long-chain polyunsaturated fatty acids (PUFAs) using these Δ5 desaturases in oleaginous yeast are disclosed.

Abstract translation: 本发明涉及具有将二硫-γ-亚麻酸（DGLA; 20：3ω-6）转化为花生四烯酸（ARA; 20：4ω-6）和/或二十碳四烯酸（ETA）的能力的Delta5去饱和酶 ; 20：4ω-3）至二十碳五烯酸（EPA; 20：5ω-3）。 公开了分离的核酸片段和包含编码Δ5去饱和酶的片段的重组构建物以及在含油酵母中使用这些Delta5去饱和酶制备长链多不饱和脂肪酸（PUFA）的方法。