中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

328 records (took 0.022 seconds)

    Amino-terminal deblocking enzyme
    41.
    发明授权
    Amino-terminal deblocking enzyme 失效
    氨基末端解封酶

    公开(公告)号：US06194190B1

    公开(公告)日：2001-02-27

    申请号：US09202832

    申请日：1998-12-21

    Applicant: Yukiko Izu ,  Tetsuki Tanaka ,  Masaru Miyagi ,  Tetsuo Tanigawa ,  Jun Tomono ,  Susumu Tsunasawa ,  Ikunoshin Kato

    Inventor： Yukiko Izu ,  Tetsuki Tanaka ,  Masaru Miyagi ,  Tetsuo Tanigawa ,  Jun Tomono ,  Susumu Tsunasawa ,  Ikunoshin Kato

    IPC: C12N950

    CPC classification number: C12N9/48

    Abstract: To provide an amino terminal protecting group-releasing enzyme characterized in that the enzyme possesses an activity for releasing a protecting group by acting on a peptide of which amino terminal is blocked by the protecting group, and exhibits the activity for two or more protecting groups, or a functional equivalent thereof; a DNA encoding the same; a method for producing the enzyme; a method for removing amino terminal protecting group including the step of subjecting to a reaction with the enzyme to release amino terminal protecting group; and a method for analyzing an amino acid sequence. The above enzyme is useful in the analysis of an amino acid sequence of peptides, particularly proteins and peptides, of which amino terminal is blocked by unknown protecting groups.

    Abstract translation: 提供氨基末端保护基释放酶，其特征在于该酶具有通过作用于氨基末端被保护基阻断的肽而释放保护基的活性，并表现出两个或更多个保护基的活性， 或其功能等同物; 编码该DNA的DNA; 一种生产该酶的方法; 一种除去氨基末端保护基的方法，包括与酶反应释放氨基末端保护基的步骤; 以及分析氨基酸序列的方法。 上述酶可用于分析肽，特别是蛋白质和肽的氨基酸序列，其氨基末端被未知保护基团阻断。

    Plasmid
    42.
    发明授权
    Plasmid 失效
    质粒

    公开(公告)号：US6165749A

    公开(公告)日：2000-12-26

    申请号：US952089

    申请日：1997-11-10

    Applicant: Hiroaki Sagawa ,  Harumi Ueno ,  Atsushi Oshima ,  Ikunoshin Kato

    Inventor： Hiroaki Sagawa ,  Harumi Ueno ,  Atsushi Oshima ,  Ikunoshin Kato

    IPC: C12N15/09 ,  C12N1/21 ,  C12N9/10 ,  C12N9/16 ,  C12N9/22 ,  C12N15/55 ,  C12N15/69 ,  C12R1/19 ,  C12P21/06

    CPC classification number: C12N15/69 ,  C12N9/1007 ,  C12N9/22

    Abstract: A plasmid vector characterized by comprising a promoter sequence that can be recognized by an RNA polymerase which is not inherent in a host and that controls the expression of desired genes and a replication origin that increases the number of copies under the induction by exogenous factors; methods for expression and isolation of target genes by using the vector; a polypeptide having the activity of an AccIII restriction endonuclease; and a DNA encoding the polypeptide. The invention provides for the first time a plasmid vector which can introduce an exogenous desired gene encoding proteins which are lethal or harmful to hosts into the hosts, a method for efficiently expressing the proteins by using the vector, and also a method for permitting a restriction endonuclease gene constituting a restriction-modification system to be isolated even in the absence of a modification enzyme gene, which has been difficult in the prior arts.

    Abstract translation: PCT No.PCT / JP97 / 00748 Sec。 371日期：1997年11月10日 102（e）日期1997年11月10日PCT 1997年3月10日PCT公布。 第WO97 / 34006号公报 日期1997年9月18日一种质粒载体，其特征在于包含可由RNA聚合酶识别的启动子序列，其不是宿主中固有的并且控制所需基因的表达和增加诱导下拷贝数的复制起点 由外生因素; 通过使用载体表达和分离靶基因的方法; 具有AccIII限制性内切核酸酶活性的多肽; 和编码该多肽的DNA。 本发明首次提供能够将编码对宿主具有致死性或有害性的蛋白质的外源性所需基因导入宿主的质粒载体，使用该载体有效表达蛋白质的方法，以及允许限制的方法 构成即使在不存在修饰酶基因的情况下分离的限制性修饰系统的核酸内切酶基因，这在现有技术中是困难的。

    Cyclopentenone derivative
    43.
    发明授权
    Cyclopentenone derivative 失效
    环戊烯酮衍生物

    公开(公告)号：US6136854A

    公开(公告)日：2000-10-24

    申请号：US359069

    申请日：1999-07-21

    Applicant: Nobuto Koyama ,  Katsushige Ikai ,  Eiji Kobayashi ,  Ikunoshin Kato

    Inventor： Nobuto Koyama ,  Katsushige Ikai ,  Eiji Kobayashi ,  Ikunoshin Kato

    IPC: C07C69/013 ,  A61K31/235 ,  C07C69/52 ,  C07C69/76

    CPC classification number: C07C69/013

    Abstract: Cyclopentenone derivative having a structure of 5-(R.sub.1 COO--)-4-(R.sub.2 COO--)--substituted 2-cyclopenten-1-one (R.sub.1 and R.sub.2 are some or different and each is alkyl group, alkenyl group or aryl group) or an optically active substance thereof; a method for the manufacture of the cyclopentenone derivative by the reaction of 4,5-dihydroxy-2-cyclopenten-1-one with the corresponding carboxylic acid or a reactive derivative thereof; and anticancer agent, apoptosis-inducing agent and antibacterial agent containing said derivative.

    Abstract translation: 具有5-（R 1 COO - ） - 4-（R 2 COO - ） - 取代的2-环戊烯-1-酮的结构的环戊烯酮衍生物（R 1和R 2有一些或不同，各自为烷基，烯基或芳基）或 其光学活性物质; 通过4,5-二羟基-2-环戊烯-1-酮与相应的羧酸或其活性衍生物的反应制备环戊烯酮衍生物的方法; 和抗癌剂，凋亡诱导剂和含有所述衍生物的抗菌剂。

    Hyperthermostable .beta.-galactosidase gene, enzyme encoded thereby, and process for production
    48.
    发明授权
    Hyperthermostable .beta.-galactosidase gene, enzyme encoded thereby, and process for production 失效
    超热稳定性β-半乳糖苷酶基因，由此编码的酶，以及生产方法

    公开(公告)号：US5744345A

    公开(公告)日：1998-04-28

    申请号：US489733

    申请日：1995-06-14

    Applicant: Atsushi Shimada ,  Miki Odate ,  Nobuto Koyama ,  Kimikazu Hashino ,  Kiyozo Asada ,  Ikunoshin Kato

    Inventor： Atsushi Shimada ,  Miki Odate ,  Nobuto Koyama ,  Kimikazu Hashino ,  Kiyozo Asada ,  Ikunoshin Kato

    IPC: C12N9/38 ,  C12N15/56

    CPC classification number: C12Y302/01023 ,  C12N9/2471

    Abstract: An isolated hyperthermostable .beta.-galactosidase gene derived from Pyrococcus furiosus is described. Examples include DNA sequences shown in SEQ ID NO. 2 and SEQ ID NO. 4 and DNAs hybridizable with these DNAs. A method of cloning the hyperthermostable .beta.-galactosidase gene in which each of the above sequences or portions thereof is used as a probe or primer is described. A process for producing a hyperthermostable .beta.-galactosidase by culturing a transformant into which a plasmid containing each of the above genes has been introduced is described. A particularly preferred gene encodes an SDS-resistant hyperthermostable .beta.-galactosidase.

    Abstract translation: 描述了源自激烈热球菌的分离的超热稳定性β-半乳糖苷酶基因。 实例包括SEQ ID NO： 2和SEQ ID NO。 4和DNA可与这些DNA杂交。 描述了将上述每个上述序列或其部分用作探针或引物的克隆超热稳定性β-半乳糖苷酶基因的方法。 描述了通过培养其中已经引入了含有上述每种基因的质粒的转化体来生产超热稳定性β-半乳糖苷酶的方法。 特别优选的基因编码SDS-抗性超热稳定性β-半乳糖苷酶。

    Aureobasidins
    49.
    发明授权
    Aureobasidins 失效

    公开(公告)号：US5698670A

    公开(公告)日：1997-12-16

    申请号：US505255

    申请日：1995-08-16

    Applicant: Toru Kurome ,  Tetsuya Inoue ,  Kazutoh Takesako ,  Kaoru Inami ,  Ikunoshin Kato

    Inventor： Toru Kurome ,  Tetsuya Inoue ,  Kazutoh Takesako ,  Kaoru Inami ,  Ikunoshin Kato

    IPC: A61K38/00 ,  C07K11/02 ,  A61K38/12 ,  C07K5/00

    CPC classification number: C07K11/02 ,  A61K38/00

    Abstract: This invention relates to Aureobasidins useful as an antifungal agent having broader spectrum. The compound according to this invention includes a cyclic depsipeptide consisting of one hydroxy acid residue having A.sup.1, 3 amino acid residues having C.sup.1, F.sup.1 and H.sup.1, respectively, 4 N-methylamino acid residues having B.sup.1, D.sup.1, G.sup.1 and I.sup.1, respectively, and 1 cyclic amino acid residue having E.sup.1. The typical compound includes one wherein A.sup.1 is CH.sub.3 CH.sub.2 CH(CH.sub.3)--, B.sup.1 is (CH.sub.3).sub.2 CH--, C.sup.1 is benzyl, cyclohexylmethyl, p-fluorobenzyl, benzyloxymethyl, benzyloxybenzyl, methoxybenzyl, cyclohexylmethyloxybenzyl or the like, D.sup.1 is methyl, benzyl, hydroxymethyl or the like, E.sup.1 is --(CH.sub.2).sub.3 --, F.sup.1 is CH.sub.3 CH.sub.2 CH(CH.sub.3)--, G.sup.1 is (CH.sub.3).sub.2 CH--, H.sup.1 is (CH.sub.3).sub.2 CHCH.sub.2 -- and I.sup.1 is (CH.sub.3).sub.2 C(OH)--. The compound according to this invention has an excellent activity against not only Candida but also Cryptococcus and Aspergillus.

    Abstract translation: PCT No.PCT / JP94 / 02201 Sec。 371日期：1995年8月16日 102（e）日期1995年8月16日PCT 1994年12月26日PCT公布。 出版物WO95 / 18147 日期1995年7月6日本发明涉及可用作具有更宽光谱的抗真菌剂的Aureobasidins。 根据本发明的化合物包括由一个具有A1的羟基酸残基，具有C1，F1和H1的3个氨基酸残基，分别具有B1，D1，G1和I1的4个N-甲基氨基酸残基的环状缩肽，以及 1个具有E1的环状氨基酸残基。 典型的化合物包括其中A 1为CH 3 CH 2 CH（CH 3） - ，B 1为（CH 3）2 CH-，C 1为苄基，环己基甲基，对氟苄基，苄氧基甲基，苄氧基苄基，甲氧基苄基，环己基甲氧基苄基等的化合物，D1为甲基，苄基， 等等，E1是 - （CH 2）3 - ，F1是CH 3 CH 2 CH（CH 3） - ，G 1是（CH 3）2 CH-，H 1是（CH 3）2 CHCH 2 - 且I 1是（CH 3）2 C（OH） - 。 根据本发明的化合物对假丝酵母不仅具有优异的活性，而且对隐球菌和曲霉也具有优异的活性。

Patent Agency Ranking