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48 条记录 (耗时 0.201 秒)

    Amino-terminal deblocking enzyme
    1.
    发明授权
    Amino-terminal deblocking enzyme 失效
    氨基末端解封酶

    公开(公告)号：US06194190B1

    公开(公告)日：2001-02-27

    申请号：US09202832

    申请日：1998-12-21

    申请人： Yukiko Izu ,  Tetsuki Tanaka ,  Masaru Miyagi ,  Tetsuo Tanigawa ,  Jun Tomono ,  Susumu Tsunasawa ,  Ikunoshin Kato

    发明人： Yukiko Izu ,  Tetsuki Tanaka ,  Masaru Miyagi ,  Tetsuo Tanigawa ,  Jun Tomono ,  Susumu Tsunasawa ,  Ikunoshin Kato

    IPC分类号： C12N950

    CPC分类号： C12N9/48

    摘要： To provide an amino terminal protecting group-releasing enzyme characterized in that the enzyme possesses an activity for releasing a protecting group by acting on a peptide of which amino terminal is blocked by the protecting group, and exhibits the activity for two or more protecting groups, or a functional equivalent thereof; a DNA encoding the same; a method for producing the enzyme; a method for removing amino terminal protecting group including the step of subjecting to a reaction with the enzyme to release amino terminal protecting group; and a method for analyzing an amino acid sequence. The above enzyme is useful in the analysis of an amino acid sequence of peptides, particularly proteins and peptides, of which amino terminal is blocked by unknown protecting groups.

    摘要翻译： 提供氨基末端保护基释放酶，其特征在于该酶具有通过作用于氨基末端被保护基阻断的肽而释放保护基的活性，并表现出两个或更多个保护基的活性， 或其功能等同物; 编码该DNA的DNA; 一种生产该酶的方法; 一种除去氨基末端保护基的方法，包括与酶反应释放氨基末端保护基的步骤; 以及分析氨基酸序列的方法。 上述酶可用于分析肽，特别是蛋白质和肽的氨基酸序列，其氨基末端被未知保护基团阻断。

    Method for effecting site-directed mutagenesis
    2.
    发明授权
    Method for effecting site-directed mutagenesis 失效
    进行定点突变的方法

    公开(公告)号：US06448048B1

    公开(公告)日：2002-09-10

    申请号：US09214146

    申请日：1998-12-29

    申请人： Jun Tomono ,  Akihiko Kita ,  Susumu Tsunasawa ,  Ikunoshin Kato

    发明人： Jun Tomono ,  Akihiko Kita ,  Susumu Tsunasawa ,  Ikunoshin Kato

    IPC分类号： C12N1564

    CPC分类号： C12N15/102

    摘要： A method for performing site-directed mutagenesis characterized in that the method includes the step of carrying out PCR by the use of a double-stranded DNA vector having one or more amber codons, the vector resulting from insertion of a target DNA fragment for site-directed mutagenesis, and at least two kinds of selection primers; and a kit for site-directed mutagenesis for use in the above method, characterized in that the kit includes amber codon reversion primers. According to the present invention, there can be provided a method for performing site-directed mutagenesis and a kit, which is useful for genetic engineering or protein engineering, more simply and rapidly. By using the method and the kit of the present invention, it is possible to efficiently obtain a mutation-introduced gene at the desired position by simply transforming a host with a PCR product obtained by PCR.

    摘要翻译： 一种用于进行定点诱变的方法，其特征在于该方法包括通过使用具有一个或多个琥珀密码子的双链DNA载体进行PCR的步骤，所述双链DNA载体由插入靶DNA片段所产生的载体， 定向诱变和至少两种选择引物; 以及用于上述方法的用于定点诱变的试剂盒，其特征在于所述试剂盒包括琥珀密码子反转引物。 根据本发明，可以提供用于进行定点诱变的方法和用于遗传工程或蛋白质工程的试剂盒，其更简单和快速。 通过使用本发明的方法和试剂盒，通过用PCR获得的PCR产物简单地转化宿主，可以有效地获得所需位置的突变导入基因。

    Polypeptide Having Rnase III Activity
    8.
    发明申请
    Polypeptide Having Rnase III Activity 审中-公开
    具有Rnase III活性的多肽

    公开(公告)号：US20070218524A1

    公开(公告)日：2007-09-20

    申请号：US10573381

    申请日：2004-09-29

    申请人： Jun Tomono ,  Harumi Ueno ,  Hiroaki Sagawa ,  Ikunoshin Kato

    发明人： Jun Tomono ,  Harumi Ueno ,  Hiroaki Sagawa ,  Ikunoshin Kato

    IPC分类号： C12P21/00 ,  C07H21/02 ,  C07K2/00

    CPC分类号： C12N15/111 ,  C12N9/22 ,  C12N2310/14 ,  C12N2330/30 ,  C12Y301/26003

    摘要： A polypeptide having an RNase III activity with which the length of a dsRNA degradation product can be easily controlled depending on reaction conditions and, in preparing a dsRNA having a length allowing it to serve as an siRNA in RNA interference, a low-molecular weight product having little RNA interferring effect is scarcely formed; a method of degrading a dsRNA with the use of the above polypeptide; and a composition and a kit for the above method.

    摘要翻译： 可以根据反应条件容易地控制dsRNA降解产物的长度的具有RNase III活性的多肽，并且在制备具有允许其在RNA干扰中作为siRNA的长度的dsRNA时，低分子量产物 几乎没有形成RNA干扰效果; 使用上述多肽降解dsRNA的方法; 以及用于上述方法的组合物和试剂盒。

    Methods of degrading dsrna and synthesizing rna
    9.
    发明申请
    Methods of degrading dsrna and synthesizing rna 审中-公开
    降解dsrna并合成rna的方法

    公开(公告)号：US20070105113A1

    公开(公告)日：2007-05-10

    申请号：US10567731

    申请日：2004-08-10

    申请人： Hiroaki Sagawa ,  Jun Tomono ,  Harumi Ueno ,  Ikunoshin Kato

    发明人： Hiroaki Sagawa ,  Jun Tomono ,  Harumi Ueno ,  Ikunoshin Kato

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C12N9/22

    CPC分类号： C12N9/22 ,  C12P19/34

    摘要： A protein having an activity of degrading a dsRNA, namely, being capable of acting on a long-chain dsRNA to form a dsRNA of a definite length; a method of efficiently preparing a dsRNA of a definite length which comprises treating a dsRNA with the protein having an activity of degrading a dsRNA in the coexistence of a protein having an activity of binding to a nucleic acid such as a protein having an RNA-binding activity; and a method of using the protein having an activity of binding to a nucleic acid to elevate the efficiency in an RNA synthesis reaction typified by dsRNA synthesis.

    摘要翻译： 具有降解dsRNA活性的蛋白质，即能够作用于长链dsRNA以形成一定长度的dsRNA; 一种有效制备一定长度的dsRNA的方法，该方法包括在具有与核酸如具有RNA结合性的蛋白质结合的蛋白质共存的蛋白质共同存在下具有降解dsRNA活性的蛋白质对dsRNA进行处理 活动; 以及使用具有与核酸结合的活性的蛋白质以提高以dsRNA合成为代表的RNA合成反应中的效率的方法。