公开(公告)号：US09725730B2

公开(公告)日：2017-08-08

申请号：US14587762

申请日：2014-12-31

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Manju Gupta ,  Terry R. Wright ,  Susan M. Jayne ,  Doug A. Smith ,  Diaa Alabed

IPC: C12N15/82 ,  C12N15/10

CPC classification number: C12N15/8216 ,  C12N15/10

Abstract: The Zea mays c.v. B73 Ubiquitin-1 (Z. mays c.v. B73 Ubi-1) promoter drives high levels of constitutive transgene expression in plants. Repeated use of the same Z. mays c.v. B73 Ubi-1 promoter in multi-gene constructs may also lead to gene silencing, thereby making transgenic products less efficacious. Provided are gene regulatory promoter elements, constructs, and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements from the Ubi-1 promoter of a different Zea species, Z. luxurians v2.

公开(公告)号：US09663832B2

公开(公告)日：2017-05-30

申请号：US13656270

申请日：2012-10-19

Applicant: Dow AgroSciences LLC

Inventor： Lasantha Chandana Ubayasena ,  Zoe Ehlert ,  Manju Gupta ,  Chandra Shekara A. Channabasavaradhya

IPC: C12Q1/68

CPC classification number: C12Q1/6895 ,  C12Q2600/156

Abstract: The subject disclosure relates in part to endpoint TaqMan® PCR assays for the detection and high throughput zygosity analysis of the fad-3c gene in canola. The subject disclosure further relates, in part, to the use of wild type DNA as a reference for use in determining zygosity. These and other related procedures can be used to uniquely identify the zygosity and variety of canola lines comprising the subject gene. The subject disclosure also provides related kits for determining zygosity from a sample of a canola plant or seed, for example.

公开(公告)号：US09650641B2

公开(公告)日：2017-05-16

申请号：US14604510

申请日：2015-01-23

Applicant: Dow AgroSciences LLC

Inventor： Manju Gupta ,  Sara Bennett ,  Navin Elango ,  Karthik Muthuraman ,  Jeffrey Beringer ,  Huixia Wu

IPC: C12N15/82

CPC classification number: C12N15/8223 ,  C12N15/8218 ,  C12N15/8271 ,  C12N15/8279

Abstract: Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using Zea mays chlorophyll a/b binding gene regulatory elements.

公开(公告)号：US09631201B2

公开(公告)日：2017-04-25

申请号：US14089260

申请日：2013-11-25

Applicant: Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

Inventor： Russell DeKelver ,  Manju Gupta ,  Jeffrey C. Miller ,  Stephen Novak ,  Joseph F. Petolino

IPC: C12N15/82 ,  C07K14/415 ,  C12N9/22

CPC classification number: C12N15/8218 ,  C07K14/415 ,  C12N9/22 ,  C12N15/8216 ,  C12N15/8247 ,  C12Y203/01041 ,  C12Y301/00

Abstract: The present disclosure relates to engineered zinc finger proteins that target genes in plants involved in fatty acid biosynthesis. Methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification are also provided.

公开(公告)号：US09523098B2

公开(公告)日：2016-12-20

申请号：US13875992

申请日：2013-05-02

Applicant: Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

Inventor： Vipula Shukla ,  Manju Gupta ,  Fyodor Urnov ,  Dmitry Guschin ,  Michiel Jan De Both ,  Paul Bundock ,  Lakshmi Sastry-Dent

IPC: C12N15/82 ,  A01C14/00 ,  C12N9/04

CPC classification number: C12N9/0006 ,  A01C14/00 ,  C12N15/8213 ,  C12N15/8241 ,  C12N15/8243 ,  C12N15/8261 ,  C12N15/8269 ,  C12Y101/01037 ,  C12Y301/00 ,  Y02A40/146

Abstract: Disclosed herein are methods and compositions for targeted modification of one or more endogenous plant malate dehydrogenase genes.

Abstract translation: 本文公开了用于靶向修饰一种或多种内源性植物苹果酸脱氢酶基因的方法和组合物。

公开(公告)号：US09150877B2

公开(公告)日：2015-10-06

申请号：US13653602

申请日：2012-10-17

Applicant: Dow AgroSciences LLC

Inventor： Manju Gupta ,  Sean Michael Russell ,  Liu Yin Shen ,  Sivarama Reddy Chennareddy ,  Jyoti Rout ,  Stephen Novak

IPC: C12N15/113 ,  C12N15/82 ,  C12N9/10

CPC classification number: C12N15/8278 ,  C07K2319/00 ,  C07K2319/81 ,  C12N9/1092 ,  C12N15/113 ,  C12N15/8243 ,  C12N15/8274 ,  C12N15/8275 ,  C12N2830/205 ,  C12Y205/01019

Abstract: Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues using a disclosed bidirectional promoter from Brassica napus or Brassica bidirectional constitutive promoter (BBCP). The constructs provided comprise at least one such bi-directional promoter linked to multiple gene expression cassettes, wherein each of the gene expression cassettes comprises at least one transgene. In some embodiments, the constructs and methods provided allow expression of genes between two and twenty.

Abstract translation: 提供了使用公开的来自甘蓝型油菜或芸苔属双侧组成型启动子（BBCP）的双向启动子在植物细胞和/或植物组织中表达多个基因的构建体和方法。 提供的构建体包含至少一个与多个基因表达盒连接的双向启动子，其中每个基因表达盒包含至少一个转基因。 在一些实施方案中，提供的构建体和方法允许在二至二十个之间表达基因。

公开(公告)号：US20150203856A1

公开(公告)日：2015-07-23

申请号：US14416811

申请日：2013-08-08

Applicant: Dow AgroSciences LLC

Inventor： Sandeep Kumar ,  Manju Gupta ,  Diaa Alabed

IPC: C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/8218

Abstract: Provided are methods, vectors and gene constructs for enhancing expression of a recombinant nucleic acid sequence in transgenic plants and plant tissues. According to the present invention, nucleic acid sequences are obtained and/or derived from the 3′ untranslated regions of genes encoding ubiquitin proteins and engineered to flank respective portions of a selected coding region of a vector. The vector construct may be introduced into plants and/or plant tissues through conventional or gene targeting procedures, resulting in enhanced expression of the selected coding region. In some embodiments, the selected coding region is a chimeric gene or gene fragment expressing one or more proteins known to impart a level of insecticidal activity to a transgenic plant and/or plant tissue.

Abstract translation: 提供了用于增强转基因植物和植物组织中重组核酸序列表达的方法，载体和基因构建体。 根据本发明，获得核酸序列和/或衍生自编码泛素蛋白的基因的3'非翻译区并且被工程化成位于载体的选定编码区的相应部分。 载体构建体可以通过常规或基因靶向程序引入植物和/或植物组织，导致所选编码区的表达增强。 在一些实施方案中，所选择的编码区是表达一种或多种已知赋予转基因植物和/或植物组织杀虫活性水平的蛋白质的嵌合基因或基因片段。

公开(公告)号：US20150064790A1

公开(公告)日：2015-03-05

申请号：US14538609

申请日：2014-11-11

Applicant: Dow AgroSciences LLC ,  Sangamo BioSciences, Inc.

Inventor： Manju Gupta ,  Asha M. Palta ,  Stephen Novak ,  Fyodor Urnov ,  Sunita Gopalan

IPC: C12N15/82

CPC classification number: C12N15/8213 ,  C07K2319/00 ,  C07K2319/81 ,  C12N9/1092 ,  C12N15/8243 ,  C12N15/8274

Abstract: The present disclosure relates to engineered zinc finger proteins that target 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) genes in plants and methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification. In particular, the disclosure pertains to zinc finger nucleases for targeted cleavage and alteration of EPSPS genes.

Abstract translation: 本公开涉及靶向植物中5-烯醇丙酮酸莽草酸-3-磷酸合酶（EPSPS）基因的工程化锌指蛋白，以及使用这种锌指蛋白调节基因表达，基因失活和靶向基因修饰的方法。 特别地，本公开涉及用于靶向切割和改变EPSPS基因的锌指核酸酶。

公开(公告)号：US20130254943A1

公开(公告)日：2013-09-26

申请号：US13674606

申请日：2012-11-12

Applicant: DOW AGROSCIENCES LLC

Inventor： Sandeep Kumar ,  Diaa Alabed ,  Terry Wright ,  Manju Gupta

IPC: C12N15/82

CPC classification number: C12N15/8216 ,  C12N15/8222 ,  C12N15/8286 ,  Y02A40/162

Abstract: Provided are constructs and methods for expressing multiple genes in plant cells and/or plant tissues. The constructs provided comprise at least one bi-directional promoter link to multiple gene expression cassettes. In some embodiments, the constructs and methods provided employ a bi-directional promoter based on a minimal core promoter element from a Zea mays Ubiquitin-1 gene, or a functional equivalent thereof. In some embodiments, the constructs and methods provided allow expression of genes between three and twenty.

Abstract translation: 提供了用于在植物细胞和/或植物组织中表达多个基因的构建体和方法。 提供的构建物包含至少一个双重启动子连接到多个基因表达盒。 在一些实施方案中，提供的构建体和方法使用基于来自玉米泛素-1基因的最小核心启动子元件或其功能等同物的双向启动子。 在一些实施方案中，提供的构建体和方法允许在3至20个之间表达基因。

公开(公告)号：US20220010374A1

公开(公告)日：2022-01-13

申请号：US17350264

申请日：2021-06-17

Applicant: E. I. DuPont de Nemours and Company ,  Pioneer Hi-Bred International, Inc. ,  Dow Agrosciences LLC

Inventor： James Wayne Bing ,  Robert F. Cressman, Jr. ,  Manju Gupta ,  Salim M. Hakimi ,  David Hondred ,  Todd L. Krone ,  Mary E. Hartnett Locke ,  Abigail K. Luckring ,  Sandra E. Meyer ,  Daniel Moellenbeck ,  Kenneth Edwin Narva ,  Paul D. Olson ,  Craig D. Sanders ,  Jimei Wang ,  Jian Zhang ,  Gan-Yuan Zhong

IPC: C12Q1/6876 ,  C12N15/82 ,  C12Q1/6895 ,  C07K14/415

Abstract: The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DAS-59122-7 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided.