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    SEMI-SYNTHETIC TEREPHTHALIC ACID VIA MICROORGANISMS THAT PRODUCE MUCONIC ACID
    44.
    发明申请
    SEMI-SYNTHETIC TEREPHTHALIC ACID VIA MICROORGANISMS THAT PRODUCE MUCONIC ACID 审中-公开
    通过生产肌酸的微生物的半合成的正十二酸

    公开(公告)号：US20110124911A1

    公开(公告)日：2011-05-26

    申请号：US12851478

    申请日：2010-08-05

    申请人： Mark J. Burk ,  Robin E. Osterhout ,  Jun Sun

    发明人： Mark J. Burk ,  Robin E. Osterhout ,  Jun Sun

    IPC分类号： C07C63/26 ,  C12N1/21 ,  C12P7/44

    CPC分类号： C12P7/44 ,  C12N9/88 ,  C12Y402/01002

    摘要： The invention provides a non-naturally occurring microbial organism having a muconate pathway having at least one exogenous nucleic acid encoding a muconate pathway enzyme expressed in a sufficient amount to produce muconate. The muconate pathway including an enzyme selected from the group consisting of a beta-ketothiolase, a beta-ketoadipyl-CoA hydrolase, a beta-ketoadipyl-CoA transferase, a beta-ketoadipyl-CoA ligase, a 2-fumarylacetate reductase, a 2-fumarylacetate dehydrogenase, a trans-3-hydroxy-4-hexendioate dehydratase, a 2-fumarylacetate aminotransferase, a 2-fumarylacetate aminating oxidoreductase, a trans-3-amino-4-hexenoate deaminase, a beta-ketoadipate enol-lactone hydrolase, a muconolactone isomerase, a muconate cycloisomerase, a beta-ketoadipyl-CoA dehydrogenase, a 3-hydroxyadipyl-CoA dehydratase, a 2,3-dehydroadipyl-CoA transferase, a 2,3-dehydroadipyl-CoA hydrolase, a 2,3-dehydroadipyl-CoA ligase, a muconate reductase, a 2-maleylacetate reductase, a 2-maleylacetate dehydrogenase, a cis-3-hydroxy-4-hexendioate dehydratase, a 2-maleylacetate aminoatransferase, a 2-maleylacetate aminating oxidoreductase, a cis-3-amino-4-hexendioate deaminase, and a muconate cis/trans isomerase. Other muconate pathway enzymes also are provided. Additionally provided are methods of producing muconate.

    摘要翻译： 本发明提供了一种非天然存在的微生物，其具有一种具有至少一种外源核酸的粘蛋白途径的微生物生物，所述外源核酸编码以足够量表达的粘蛋白途径酶以产生粘蛋白。 所述粘蛋白途径包括选自β-酮硫解酶，β-酮己二酰辅酶A水解酶，β-酮己二酰辅酶A转移酶，β-酮己二酰辅酶A连接酶，2-富马酰乙酸酯还原酶，2- 富马酰乙酸脱氢酶，反式-3-羟基-4-己二酸脱水酶，2-富马酸乙酯氨基转移酶，2-富马酸乙酯胺化氧化还原酶，反式-3-氨基-4-己烯酸脱氨酶，β-酮己二酸烯醇内酯水解酶， 粘蛋白内酯异构酶，粘蛋白环异构酶，β-酮己二酰辅酶A脱氢酶，3-羟基己二酰辅酶A脱水酶，2,3-脱氢阿迪普基-CoA转移酶，2,3-脱氢阿迪普基-CoA水解酶，2,3-脱氢阿迪普酯 - CoA连接酶，粘蛋白还原酶，2-马来酰乙酸酯还原酶，2-马来酰乙酸酯脱氢酶，顺式-3-羟基-4-己二酸脱水酶，2-马来酰乙酸氨基转移酶，2-马来酰乙酸胺胺化氧化还原酶，顺式-3-氨基 -4-十六酸脱氨酶和粘蛋白顺式/反式异构酶。 还提供了其它粘蛋白途径酶。 另外提供了生产粘蛋白的方法。

    ORGANISMS FOR THE PRODUCTION OF CYCLOHEXANONE
    45.
    发明申请
    ORGANISMS FOR THE PRODUCTION OF CYCLOHEXANONE 有权
    生产环己酮的有机体

    公开(公告)号：US20110014668A1

    公开(公告)日：2011-01-20

    申请号：US12780802

    申请日：2010-05-14

    申请人： Robin E. Osterhout ,  Anthony P. Burgard ,  Mark J. Burk ,  Priti Pharkya

    发明人： Robin E. Osterhout ,  Anthony P. Burgard ,  Mark J. Burk ,  Priti Pharkya

    IPC分类号： C12P7/26 ,  C12N1/00

    CPC分类号： C12P7/26 ,  C12N9/16 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12N15/70

    摘要： A non-naturally occurring microbial organism has cyclohexanone pathways that include at least one exogenous nucleic acid encoding a cyclohexanone pathway enzyme. A pathway includes a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on C—C bond), a 2-ketocyclohexane-1-carboxylate decarboxylase and an enzyme selected from a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-1-carboxyl-CoA transferase, and a 2-ketocyclohexane-1-carboxyl-CoA synthetase. A pathway includes an enzyme selected from a 6-ketocyclohex-1-ene-1-carboxyl-CoA hydrolase (acting on C—C bond), a 6-ketocyclohex-1-ene-1-carboxyl-CoA synthetase, a 6-ketocyclohex-1-ene-1-carboxyl-CoA hydrolase (acting on thioester), a 6-ketocyclohex-1-ene-1-carboxyl-CoA transferase, a 6-ketocyclohex-1-ene-1-carboxyl-CoA reductase, a 6-ketocyclohex-1-ene-1-carboxylate decarboxylase, a 6-ketocyclohex-1-ene-1-carboxylate reductase, a 2-ketocyclohexane-1-carboxyl-CoA synthetase, a 2-ketocyclohexane-1-carboxyl-CoA transferase, a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-1-carboxylate decarboxylase, and a cyclohexanone dehydrogenase. A pathway includes an adipate semialdehyde dehydratase, a cyclohexane-1,2-diol dehydrogenase, and a cyclohexane-1,2-diol dehydratase. A pathway includes a 3-oxopimelate decarboxylase, a 4-acetylbutyrate dehydratase, a 3-hydroxycyclohexanone dehydrogenase, a 2-cyclohexenone hydratase, a cyclohexanone dehydrogenase and an enzyme selected from a 3-oxopimeloyl-CoA synthetase, a 3-oxopimeloyl-CoA hydrolase (acting on thioester), and a 3-oxopimeloyl-coA transferase. Each these pathways can include a PEP carboxykinase. A method for producing cyclohexanone includes culturing these non-naturally occurring microbial organisms.

    摘要翻译： 非天然存在的微生物有机体具有包含至少一种编码环己酮途径酶的外源核酸的环己酮途径。 途径包括2-酮环己烷-1-羧基-CoA水解酶（作用于C-C键），2-酮环己烷-1-羧酸脱羧酶和选自2-酮环己烷-1-羧基-CoA水解酶的酶（作用 在硫酯上），2-酮环己烷-1-羧基-CoA转移酶和2-酮环己烷-1-羧基-CoA合成酶。 途径包括选自6-酮环己-1-烯-1-羧基-CoA水解酶（作用于C-C键）的酶，6-酮环己-1-烯-1-羧基-CoA合成酶，6- 酮酮环己-1-烯-1-羧基-CoA水解酶（作用于硫酯），6-酮环己-1-烯-1-羧基-CoA转移酶，6-酮环己-1-烯-1-羧基-CoA还原酶， 6-酮环己-1-烯-1-羧酸脱羧酶，6-酮环己-1-烯-1-羧酸还原酶，2-酮环己烷-1-羧基-CoA合成酶，2-酮环己烷-1-羧基-CoA 转移酶，2-酮环己烷-1-羧基-CoA水解酶（作用于硫酯），2-酮环己烷-1-羧酸脱羧酶和环己酮脱氢酶。 途径包括己二酸半醛脱水酶，环己烷-1,2-二醇脱氢酶和环己烷-1,2-二醇脱水酶。 途径包括3-氧代邻苯二甲酸脱羧酶，4-乙酰基丁酸脱水酶，3-羟基环己酮脱氢酶，2-环己烯酮水合酶，环己酮脱氢酶和选自3-氧代木糖酰辅酶A合成酶，3-氧代木糖酰-CoA水解酶 （作用于硫酯）和3-氧代木糖酰辅酶A转移酶。 每个这些途径可以包括PEP羧基激酶。 生产环己酮的方法包括培养这些非天然存在的微生物。

    MICROORGANISMS FOR THE PRODUCTION OF METHYL ETHYL KETONE AND 2-BUTANOL
    47.
    发明申请
    MICROORGANISMS FOR THE PRODUCTION OF METHYL ETHYL KETONE AND 2-BUTANOL 审中-公开
    用于生产甲基乙基酮和2-丁醇的微生物

    公开(公告)号：US20100184173A1

    公开(公告)日：2010-07-22

    申请号：US12618545

    申请日：2009-11-13

    申请人： Mark J. Burk ,  Priti Pharkya ,  Anthony P. Burgard

    发明人： Mark J. Burk ,  Priti Pharkya ,  Anthony P. Burgard

    IPC分类号： C12P7/26 ,  C12N1/00 ,  C12P7/16

    CPC分类号： C12P7/16 ,  C12N15/52 ,  C12P7/26 ,  Y02E50/10

    摘要： A non-naturally occurring microbial organism having a methyl ethyl ketone pathway includes at least one exogenous nucleic acid encoding a methyl ethyl ketone pathway enzyme expressed in a sufficient amount to produce methyl ethyl ketone. The methyl ethyl ketone pathway includes a β-ketothiolase, a β-ketovalerate decarboxylase and an enzyme selected from the group consisting of a β-ketovaleryl-CoA hydrolase and a β-ketovaleryl-CoA transferase. Alternatively, the methyl ethyl ketone pathway includes a 2-methylacetoacetyl-CoA thiolase, a 2-methylacetoacetate decarboxylase and an enzyme selected from the group consisting of a 2-methylacetoacetyl-CoA hydrolase and a 2-methylacetoacetyl-CoA transferase. Either pathway can further include a methyl ethyl ketone reductase to produce 2-BuOH. A method for producing methyl ethyl ketone or 2-BuOH includes culturing these non-naturally occurring microbial organisms under conditions, and for a sufficient period of time, to produce methyl ethyl ketone or 2-BuOH.

    摘要翻译： 具有甲基乙基酮途径的非天然存在的微生物包括至少一种编码甲基乙基酮途径酶的外源核酸，其以足以产生甲基乙基酮的量表达。 甲基乙基酮途径包括α-酮硫解酶，α-酮戊二酸脱羧酶和选自α-酮戊酰基-CoA水解酶和一种α-酮戊酰基-CoA转移酶的酶。 或者，甲基乙基酮途径包括2-甲基乙酰乙酰辅酶A硫解酶，2-甲基乙酰乙酸脱羧酶和选自2-甲基乙酰乙酰辅酶A水解酶和2-甲基乙酰乙酰辅酶A转移酶的酶。 任一途径还可以包括甲基乙基酮还原酶以产生2-BuOH。 一种生产甲基乙基酮或2-BuOH的方法包括在条件和足够的时间内培养这些非天然存在的微生物生产甲基乙基酮或2-BuOH。

    METHODS AND ORGANISMS FOR PRODUCTION OF 3-HYDROXYPROPIONIC ACID
    48.
    发明申请
    METHODS AND ORGANISMS FOR PRODUCTION OF 3-HYDROXYPROPIONIC ACID 审中-公开
    用于生产3-羟基丙酸的方法和有机体

    公开(公告)号：US20100021978A1

    公开(公告)日：2010-01-28

    申请号：US12508547

    申请日：2009-07-23

    申请人： Mark J. Burk ,  Robin E. Osterhout

    发明人： Mark J. Burk ,  Robin E. Osterhout

    IPC分类号： C12P7/42 ,  C12N1/21

    CPC分类号： C12P7/42

    摘要： A non-naturally occurring microbial organism having a 3-hydroxypropanoic acid (3-HP) pathway includes at least one exogenous nucleic acid encoding 3-HP pathway enzyme expressed in a sufficient amount to produce 3-HP. The 3-HP pathway includes a 2-keto acid decarboxylase, a CoA-dependent oxaloacetate dehydrogenase, or a malate decarboxylase. A method for producing 3-HP includes culturing a non-naturally occurring microbial organism having a 3-HP pathway that includes at least one exogenous nucleic acid encoding a 3-HP pathway enzyme expressed in a sufficient amount to produce 3-HP under conditions and for a sufficient period of time to produce 3-HP. The 3-HP pathway includes a 2-keto acid decarboxylase, a CoA-dependent oxaloacetate dehydrogenase, or a malate decarboxylase.

    摘要翻译： 具有3-羟基丙酸（3-HP）途径的非天然存在的微生物包含至少一种编码3-HP途径酶的外源核酸，其以足以产生3-HP的量表达。 3-HP途径包括2-酮酸脱羧酶，辅酶A依赖性草酰乙酸脱氢酶或苹果酸脱羧酶。 制备3-HP的方法包括培养具有3-HP途径的非天然存在的微生物，其包括至少一种编码以足够量表达的3-HP途径酶的外源核酸以在条件下产生3-HP， 足够的时间来生产3-HP。 3-HP途径包括2-酮酸脱羧酶，辅酶A依赖性草酰乙酸脱氢酶或苹果酸脱羧酶。

    COMPOSITIONS AND METHODS FOR THE BIOSYNTHESIS OF 1,4-BUTANEDIOL AND ITS PRECURSORS
    49.
    发明申请
    COMPOSITIONS AND METHODS FOR THE BIOSYNTHESIS OF 1,4-BUTANEDIOL AND ITS PRECURSORS 有权

    公开(公告)号：US20090075351A1

    公开(公告)日：2009-03-19

    申请号：US12049256

    申请日：2008-03-14

    申请人： Mark J. Burk ,  Stephen J. Van Dien ,  Anthony P. Burgard ,  Wei Niu

    发明人： Mark J. Burk ,  Stephen J. Van Dien ,  Anthony P. Burgard ,  Wei Niu

    IPC分类号： C12N1/21 ,  C12P7/52 ,  C12P7/18

    CPC分类号： C12N15/52 ,  B01D3/002 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/88 ,  C12N9/93 ,  C12N15/70 ,  C12N15/81 ,  C12P7/18 ,  C12P7/42 ,  C12P7/52 ,  C12P17/04 ,  C12Y101/01061 ,  C12Y102/01076 ,  C12Y401/01071 ,  C12Y602/01004 ,  Y02P20/52

    摘要： The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided is a method for the production of 4-HB. The method includes culturing a non-naturally occurring microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase or α-ketoglutarate decarboxylase under substantially anaerobic conditions for a sufficient period of time to produce monomeric 4-hydroxybutanoic acid (4-HB). Further provided is a method for the production of BDO. The method includes culturing a non-naturally occurring microbial biocatalyst, comprising a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-hydroxybutyrate kinase, phosphotranshydroxybutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase for a sufficient period of time to produce 1,4-butanediol (BDO). The 4-HB and/or BDO products can be secreted into the culture medium.