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41.发明授权公开(公告)号：US07429385B2
公开(公告)日：2008-09-30
申请号：US10371040
申请日：2003-02-18
申请人： Michael Houghton , Qui-Lim Choo , Sergio Abrignani , David Chien , Mark Selby , Edward Glazer
发明人： Michael Houghton , Qui-Lim Choo , Sergio Abrignani , David Chien , Mark Selby , Edward Glazer
CPC分类号： C07K14/005 , A61K39/00 , C12N2770/24222 , Y10S977/923
摘要： Methods for obtaining recombinantly produced, C-terminally truncated, E1 and E2 polypeptides from cell lysates are disclosed. The intracellularly expressed truncated molecules display improved biological properties as compared to their secreted counterparts.
摘要翻译： 公开了从细胞裂解物中获得重组产生的C-末端截短的E1和E2多肽的方法。 与其分泌的对应物相比，细胞内表达的截短分子显示出改善的生物学特性。
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公开(公告)号：US06521423B1
公开(公告)日：2003-02-18
申请号：US09693596
申请日：2000-10-19
申请人： Michael Houghton , Qui-Lim Choo , Sergio Abrignani , David Chien , Mark Selby , Edward Glazer
发明人： Michael Houghton , Qui-Lim Choo , Sergio Abrignani , David Chien , Mark Selby , Edward Glazer
IPC分类号： C12P2100
CPC分类号： C07K14/005 , A61K39/00 , C12N2770/24222 , Y10S977/923
摘要： Methods for obtaining recombinantly produced, C-terminally truncated, E1 and E2 polypeptides from cell lysates are disclosed. The intracellularly expressed truncated molecules display improved biological properties as compared to their secreted counterparts.
摘要翻译： 公开了从细胞裂解物获得重组产生的C-末端截短的E1和E2多肽的方法。 与其分泌的对应物相比，细胞内表达的截短分子显示出改善的生物学特性。
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43.发明授权HCV NS3 protein fragments having helicase activity and improved solubility 有权具有解旋酶活性和改善溶解性的HCV NS3蛋白片段公开(公告)号：US06472180B1
公开(公告)日：2002-10-29
申请号：US09483799
申请日：2000-01-15
申请人： Michael Houghton , Qui-Lim Choo , Jang Han , Joonho Choe
发明人： Michael Houghton , Qui-Lim Choo , Jang Han , Joonho Choe
IPC分类号： C12N1562
CPC分类号： C07K14/005 , A61K38/00 , C07K14/00 , C07K2319/00 , C12N9/0089 , C12N9/506 , C12N9/90 , C12N2770/24222
摘要： The Hepatitis C Virus (HCV) NS3 protein contains amino acid motifs of a serine proteinase, a nucleotide triphosphatase (NTPase), and an RNA helicase. A carboxy fragment of the HCV NS3 protein was purified and possessed RNA helicase activity. Detections from the amino terminus resulted in the protein becoming soluble. Deletions from the carboxy terminus do not result in a loss of helicase activity until at least 50 amino acids are deleted. The helicase activity requires ATP and divalent cations such as Mg2+ and Mn2+. The helicase activity was blocked by monoclonal antibody specific to the HCV NS3 protein.
摘要翻译： 丙型肝炎病毒（HCV）NS3蛋白含有丝氨酸蛋白酶，核苷酸三磷酸酶（NTPase）和RNA解旋酶的氨基酸基序。 HCV NS3蛋白的羧基片段被纯化并具有RNA解旋酶活性。 氨基末端的检测导致蛋白质变得可溶。 从羧基末端的缺失不会导致直到至少50个氨基酸被缺失的解旋酶活性丧失。 解旋酶活性需要ATP和二价阳离子如Mg2 +和Mn2 +。 解旋酶活性被HCV NS3蛋白特异性的单克隆抗体阻断。
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公开(公告)号：US5389528A
公开(公告)日：1995-02-14
申请号：US97519
申请日：1993-07-27
IPC分类号： C12N15/09 , A61K39/00 , A61K39/29 , A61P31/12 , A61P31/14 , C07H21/04 , C07K14/00 , C07K14/02 , C07K14/08 , C07K14/10 , C07K14/18 , C07K14/435 , C07K16/00 , C07K16/10 , C12N1/21 , C12N5/10 , C12N9/02 , C12N15/00 , C12N15/02 , C12N15/51 , C12P21/00 , C12P21/02 , C12P21/08 , C12Q1/68 , C12Q1/70 , C12R1/19 , C12R1/85 , C12R1/91 , C12R1/92 , G01N33/53 , G01N33/576 , G01N33/577 , C12N15/62 , C12N15/63
CPC分类号： C12N9/0089 , C07K14/005 , C12Q1/706 , G01N33/5765 , A61K39/00 , C07K2319/00 , C07K2319/40 , C12N2760/10122 , C12N2770/28122 , Y10S530/824 , Y10S530/825 , Y10S530/826
摘要： The entire genome of the hepatitis D virus has been shown to be a circular single-stranded RNA of 1679 bases. Several open reading frames in both the genomic and complementary strands indicate possible protein products. The products encoded in one open reading frame, ORF5, are identified as viral polypeptides p24.sup..delta. and p27.sup..delta., of which the nuclear .delta. antigens in HDV infected liver is comprised. These products, as well as others encoded in ORFs 1, 2, 6, and 7 are produced in recombinant expression systems. The ORF5 products, in particular, are useful for HDV diagnosis and vaccines.
摘要翻译： D型病毒的全基因组已显示为1679个碱基的环状单链RNA。 基因组和互补链中的几个开放阅读框表示可能的蛋白质产物。 编码在一个开放阅读框ORF5中的产物被鉴定为病毒多肽p24δ和p27δ，其中包含HDV感染的肝脏中的核δ抗原包括在内。 这些产物以及编码在ORF 1,2,6和7中的其它产物均在重组表达系统中产生。 ORF5产品特别适用于HDV诊断和疫苗。
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公开(公告)号：US20120141529A1
公开(公告)日：2012-06-07
申请号：US13355192
申请日：2012-01-20
CPC分类号： C12N9/14 , A61K39/00 , A61K39/12 , A61K2039/5258 , A61K2039/53 , A61K2039/545 , A61K2039/55544 , A61K2039/55555 , C07K14/005 , C07K2319/00 , C07K2319/40 , C12N2770/16022 , C12N2770/16034
摘要： Immunogenic compositions that elicit immune responses against Norovirus and Sapovirus antigens are described. In particular, the invention relates to polynucleotides encoding one or more capsid proteins or other immunogenic viral polypeptides from one or more strains of Norovirus and/or Sapovirus, coexpression of such immunogenic viral polypeptides with adjuvants, and methods of using the polynucleotides in applications including immunization and production of immunogenic viral polypeptides and viral-like particles (VLPs). Methods for producing Norovirus- or Sapovirus-derived multiple epitope fusion antigens or polyproteins and immunogenic compositions comprising one or more immunogenic polypeptides, polynucleotides, VLPs, and/or adjuvants are also described. The immunogenic compositions of the invention may also contain antigens other than Norovirus or Sapovirus antigens, including antigens that can be used in immunization against pathogens that cause diarrheal diseases, such as antigens derived from rotavirus.
摘要翻译： 描述了引起针对诺如病毒和曙红病毒抗原的免疫应答的免疫原性组合物。 特别地，本发明涉及编码来自一种或多种诺如病毒和/或札幌病毒株的一种或多种衣壳蛋白或其它免疫原性病毒多肽的多核苷酸，这种免疫原性病毒多肽与佐剂的共表达，以及在包括免疫的应用中使用多核苷酸的方法 以及产生免疫原性病毒多肽和病毒样颗粒（VLP）。 还描述了用于生产包含一种或多种免疫原性多肽，多核苷酸，VLP和/或佐剂的诺如病毒或札幌病毒衍生的多表位融合抗原或多蛋白的免疫原性组合物的方法。 本发明的免疫原性组合物还可以含有除诺如病毒或札幌病毒抗原以外的抗原，包括可用于免疫引起腹泻疾病的病原体的抗原，例如来源于轮状病毒的抗原。
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公开(公告)号：US08142793B2
公开(公告)日：2012-03-27
申请号：US12383425
申请日：2009-03-24
CPC分类号： C12N9/14 , A61K39/00 , A61K39/12 , A61K2039/5258 , A61K2039/53 , A61K2039/545 , A61K2039/55544 , A61K2039/55555 , C07K14/005 , C07K2319/00 , C07K2319/40 , C12N2770/16022 , C12N2770/16034
摘要： Immunogenic compositions that elicit immune responses against Norovirus and Sapovirus antigens are described. In particular, the invention relates to polynucleotides encoding one or more capsid proteins or other immunogenic viral polypeptides from one or more strains of Norovirus and/or Sapovirus, coexpression of such immunogenic viral polypeptides with adjuvants, and methods of using the polynucleotides in applications including immunization and production of immunogenic viral polypeptides and viral-like particles (VLPs). Methods for producing Norovirus- or Sapovirus-derived multiple epitope fusion antigens or polyproteins and immunogenic compositions comprising one or more immunogenic polypeptides, polynucleotides, VLPs, and/or adjuvants are also described. The immunogenic compositions of the invention may also contain antigens other than Norovirus or Sapovirus antigens, including antigens that can be used in immunization against pathogens that cause diarrheal diseases, such as antigens derived from rotavirus.
摘要翻译： 描述了引起针对诺如病毒和曙红病毒抗原的免疫应答的免疫原性组合物。 特别地，本发明涉及编码来自一种或多种诺如病毒和/或札幌病毒株的一种或多种衣壳蛋白或其它免疫原性病毒多肽的多核苷酸，这种免疫原性病毒多肽与佐剂的共表达，以及在包括免疫的应用中使用多核苷酸的方法 以及产生免疫原性病毒多肽和病毒样颗粒（VLP）。 还描述了用于生产包含一种或多种免疫原性多肽，多核苷酸，VLP和/或佐剂的诺如病毒或札幌病毒衍生的多表位融合抗原或多蛋白的免疫原性组合物的方法。 本发明的免疫原性组合物还可以含有除诺如病毒或札幌病毒抗原以外的抗原，包括可用于免疫引起腹泻疾病的病原体的抗原，例如来源于轮状病毒的抗原。
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公开(公告)号：US20110014652A1
公开(公告)日：2011-01-20
申请号：US12383419
申请日：2009-03-24
IPC分类号： C12P21/02
CPC分类号： C12N9/14 , A61K39/00 , A61K39/12 , A61K2039/5258 , A61K2039/53 , A61K2039/545 , A61K2039/55544 , A61K2039/55555 , C07K14/005 , C07K2319/00 , C07K2319/40 , C12N2770/16022 , C12N2770/16034
摘要： Immunogenic compositions that elicit immune responses against Norovirus and Sapovirus antigens are described. In particular, the invention relates to polynucleotides encoding one or more capsid proteins or other immunogenic viral polypeptides from one or more strains of Norovirus and/or Sapovirus, coexpression of such immunogenic viral polypeptides with adjuvants, and methods of using the polynucleotides in applications including immunization and production of immunogenic viral polypeptides and viral-like particles (VLPs). Methods for producing Norovirus- or Sapovirus-derived multiple epitope fusion antigens or polyproteins and immunogenic compositions comprising one or more immunogenic polypeptides, polynucleotides, VLPs, and/or adjuvants are also described. The immunogenic compositions of the invention may also contain antigens other than Norovirus or Sapovirus antigens, including antigens that can be used in immunization against pathogens that cause diarrheal diseases, such as antigens derived from rotavirus.
摘要翻译： 描述了引起针对诺如病毒和曙红病毒抗原的免疫应答的免疫原性组合物。 特别地，本发明涉及编码来自一种或多种诺如病毒和/或札幌病毒株的一种或多种衣壳蛋白或其它免疫原性病毒多肽的多核苷酸，这种免疫原性病毒多肽与佐剂的共表达，以及在包括免疫的应用中使用多核苷酸的方法 以及产生免疫原性病毒多肽和病毒样颗粒（VLP）。 还描述了用于生产包含一种或多种免疫原性多肽，多核苷酸，VLP和/或佐剂的诺如病毒或札幌病毒衍生的多表位融合抗原或多蛋白的免疫原性组合物的方法。 本发明的免疫原性组合物还可以含有除诺如病毒或札幌病毒抗原以外的抗原，包括可用于免疫引起腹泻疾病的病原体的抗原，例如来源于轮状病毒的抗原。
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公开(公告)号：US20100291129A1
公开(公告)日：2010-11-18
申请号：US12383420
申请日：2009-03-24
IPC分类号： A61K39/125 , A61K39/295 , A61P31/14 , A61P31/00 , A61K39/12
CPC分类号： C12N9/14 , A61K39/00 , A61K39/12 , A61K2039/5258 , A61K2039/53 , A61K2039/545 , A61K2039/55544 , A61K2039/55555 , C07K14/005 , C07K2319/00 , C07K2319/40 , C12N2770/16022 , C12N2770/16034
摘要： Immunogenic compositions that elicit immune responses against Norovirus and Sapovirus antigens are described. In particular, the invention relates to polynucleotides encoding one or more capsid proteins or other immunogenic viral polypeptides from one or more strains of Norovirus and/or Sapovirus, coexpression of such immunogenic viral polypeptides with adjuvants, and methods of using the polynucleotides in applications including immunization and production of immunogenic viral polypeptides and viral-like particles (VLPs). Methods for producing Norovirus- or Sapovirus-derived multiple epitope fusion antigens or polyproteins and immunogenic compositions comprising one or more immunogenic polypeptides, polynucleotides, VLPs, and/or adjuvants are also described. The immunogenic compositions of the invention may also contain antigens other than Norovirus or Sapovirus antigens, including antigens that can be used in immunization against pathogens that cause diarrheal diseases, such as antigens derived from rotavirus.
摘要翻译： 描述了引起针对诺如病毒和曙红病毒抗原的免疫应答的免疫原性组合物。 特别地，本发明涉及编码来自一种或多种诺如病毒和/或札幌病毒株的一种或多种衣壳蛋白或其它免疫原性病毒多肽的多核苷酸，这种免疫原性病毒多肽与佐剂的共表达，以及在包括免疫的应用中使用多核苷酸的方法 以及产生免疫原性病毒多肽和病毒样颗粒（VLP）。 还描述了用于生产包含一种或多种免疫原性多肽，多核苷酸，VLP和/或佐剂的诺如病毒或札幌病毒衍生的多表位融合抗原或多蛋白的免疫原性组合物的方法。 本发明的免疫原性组合物还可以含有除诺如病毒或札幌病毒抗原以外的抗原，包括可用于免疫引起腹泻疾病的病原体的抗原，例如来源于轮状病毒的抗原。
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公开(公告)号：US20100262178A1
公开(公告)日：2010-10-14
申请号：US12824343
申请日：2010-06-28
CPC分类号： A61M29/02 , A61M25/1029 , A61M2025/1075 , A61M2025/1084
摘要： The present invention provides a balloon which achieves diameters greater than 6 mm for securement to a catheter. The balloon includes an elastomeric generally hollow pressure expandable body which exhibits essentially radial symmetry and constant length when expanded under an internally applied minimum working pressure from an uninflated state.
摘要翻译： 本发明提供一种实现直径大于6mm的气囊，用于固定到导管。 气囊包括弹性体大体中空压力可膨胀体，其在内部施加的最小工作压力下从未充气状态膨胀时，其基本上呈径向对称性和恒定长度。
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公开(公告)号：US07785290B2
公开(公告)日：2010-08-31
申请号：US11501190
申请日：2006-08-07
IPC分类号： A61M31/00
CPC分类号： A61M29/02 , A61M25/1029 , A61M2025/1075 , A61M2025/1084
摘要： The present invention provides a balloon which achieves diameters greater than 6 mm for securement to a catheter. The balloon includes an elastomeric generally hollow pressure expandable body which exhibits essentially radial symmetry and constant length when expanded under an internally applied minimum working pressure from an uninflated state.
摘要翻译： 本发明提供一种实现直径大于6mm的气囊，用于固定到导管。 气囊包括弹性体大体中空压力可膨胀体，其在内部施加的最小工作压力下从未充气状态膨胀时，其基本上呈径向对称性和恒定长度。
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