公开(公告)号：US07462469B2

公开(公告)日：2008-12-09

申请号：US10154936

申请日：2002-05-23

申请人： Steven H. Bass ,  S. Christopher Davis ,  Phillip A. Patten ,  Matthew Tobin ,  Jeremy Minshull ,  Mark Welch ,  Claes Gustafsson ,  Brian Carr ,  Stephane Jenne ,  Sun Ai Raillard ,  Andreas Crameri ,  Willem P. C. Stemmer ,  Robin Emig ,  Pascal Longchamp ,  Stanley Goldman ,  Lorraine J. Giver ,  Joseph A. Affholter

发明人： Steven H. Bass ,  S. Christopher Davis ,  Phillip A. Patten ,  Matthew Tobin ,  Jeremy Minshull ,  Mark Welch ,  Claes Gustafsson ,  Brian Carr ,  Stephane Jenne ,  Sun Ai Raillard ,  Andreas Crameri ,  Willem P. C. Stemmer ,  Robin Emig ,  Pascal Longchamp ,  Stanley Goldman ,  Lorraine J. Giver ,  Joseph A. Affholter

IPC分类号： C12P19/34 ,  C12Q1/68

CPC分类号： C12N15/1031 ,  B01J19/0046 ,  B01J2219/00378 ,  B01J2219/00585 ,  B01J2219/00659 ,  B01J2219/00675 ,  B01J2219/00689 ,  B01J2219/00695 ,  B01J2219/007 ,  B01J2219/00722 ,  B82Y30/00 ,  C12N15/1027 ,  C40B60/04 ,  G06F19/18 ,  Y02A90/22

摘要： Integrated systems and methods for diversity generation and screening are provided. The systems use common fluid and array handling components to provide nucleic acid diversification, transcription, translation, product screening and subsequent diversification reactions.

摘要翻译： 提供了用于多样化生成和筛选的集成系统和方法。 该系统使用普通液体和阵列处理组分来提供核酸多样化，转录，翻译，产物筛选和随后的多样化反应。

公开(公告)号：US07384387B1

公开(公告)日：2008-06-10

申请号：US09502283

申请日：2000-02-11

申请人： Sun Ai Raillard ,  Yong Hong Chen ,  Claus Krebber ,  Jeremy Minshull ,  Willem P. C. Stemmer

发明人： Sun Ai Raillard ,  Yong Hong Chen ,  Claus Krebber ,  Jeremy Minshull ,  Willem P. C. Stemmer

IPC分类号： C40B30/10

CPC分类号： C12Q1/04 ,  G01N33/5005

摘要： Apparatus and methods for high throughput mass spectrometry are provided. The methods involve sample preparation in an off-line parallel purification system. Such methods include but are not limited to the use of an appropriate buffer when generating samples or the use of a solid support for tagged components. The samples prepared in this way do not then need to be column separated. The apparatus provided includes a cell growth plate for growing cells and generating products and/or reactants, an off-line parallel purification system, a mass spectrometer, and an automatic sampler that transports samples and injects them into the mass spectrometer of the apparatus. The methods and apparatus described are used, for example, in screening enzyme reaction pathways.

摘要翻译： 提供了用于高通量质谱的装置和方法。 该方法涉及离线并行纯化系统中的样品制备。 这样的方法包括但不限于在产生样品时使用适当的缓冲液或者使用固体支持物用于标记的组分。 以这种方式制备的样品不需要进行柱分离。 提供的装置包括用于生长细胞并产生产物和/或反应物的细胞生长板，离线平行纯化系统，质谱仪和自动采样器，其将样品输送并注入到装置的质谱仪中。 所述的方法和装置例如用于筛选酶反应途径。

公开(公告)号：US07288375B2

公开(公告)日：2007-10-30

申请号：US10623036

申请日：2003-07-18

申请人： Willem P. C. Stemmer ,  Andreas Crameri

发明人： Willem P. C. Stemmer ,  Andreas Crameri

IPC分类号： C12Q1/68 ,  C12P21/02 ,  C12P19/34 ,  C12P21/08 ,  C07H21/02

CPC分类号： C40B40/02 ,  C07K14/43595 ,  C07K14/545 ,  C07K16/00 ,  C07K2317/565 ,  C07K2317/622 ,  C12N9/2471 ,  C12N9/86 ,  C12N15/1027 ,  C12N15/1034 ,  C12N15/1037 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Y302/01023 ,  C12Y305/02006 ,  C40B40/08 ,  C40B50/06

摘要： A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.

摘要翻译： 描述了随机断裂后DNA重组的方法及其通过体外或体内重组诱变核酸序列的应用。 特别地，描述了用于生产编码突变蛋白的核酸片段或多核苷酸的方法。 本发明还涉及一种反复循环诱变，洗牌和选择的方法，其允许蛋白质的体外或体内的定向分子进化。

公开(公告)号：US06995017B1

公开(公告)日：2006-02-07

申请号：US09239395

申请日：1999-01-28

申请人： Willem P. C. Stemmer

发明人： Willem P. C. Stemmer

IPC分类号： C12N15/00 ,  C12Q1/68 ,  C07H21/02 ,  C07H21/04

CPC分类号： C12N15/1027 ,  C12N15/1034

摘要： A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.

公开(公告)号：US06902922B2

公开(公告)日：2005-06-07

申请号：US09824893

申请日：2001-04-02

申请人： Jon E. Ness ,  Mark Welch ,  Lorraine J. Giver ,  Joel R. Cherry ,  Torben V. Borchert ,  Willem P. C. Stemmer ,  Jeremy Minshull

发明人： Jon E. Ness ,  Mark Welch ,  Lorraine J. Giver ,  Joel R. Cherry ,  Torben V. Borchert ,  Willem P. C. Stemmer ,  Jeremy Minshull

IPC分类号： C12N15/09 ,  C07K16/40 ,  C11D3/386 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/54 ,  C12N9/56 ,  C12N15/57 ,  C12N9/50 ,  C12N15/74

CPC分类号： C12N9/6408 ,  C12N9/54

摘要： New subtilisin homologues (both nucleic acids and proteins) are provided. Compositions which include these new proteins, recombinant cells, shuffling methods involving the new homologues, antibodies to the new homologues, and methods of using the homologues are also provided.

摘要翻译： 提供了新的枯草杆菌蛋白酶同源物（核酸和蛋白质）。 还提供了包括这些新蛋白质，重组细胞，涉及新同系物的洗牌方法，新同系物的抗体以及使用同源物的方法的组合物。

公开(公告)号：US06541011B2

公开(公告)日：2003-04-01

申请号：US09247890

申请日：1999-02-10

申请人： Juha Punnonen ,  Steven H. Bass ,  Robert Gerald Whalen ,  Russell Howard ,  Willem P. C. Stemmer

发明人： Juha Punnonen ,  Steven H. Bass ,  Robert Gerald Whalen ,  Russell Howard ,  Willem P. C. Stemmer

IPC分类号： A61K3912

CPC分类号： C40B40/02 ,  A61K39/00 ,  A61K39/0011 ,  A61K39/015 ,  A61K39/0225 ,  A61K39/0258 ,  A61K39/0291 ,  A61K39/085 ,  A61K39/105 ,  A61K39/107 ,  A61K39/12 ,  A61K39/35 ,  A61K2039/53 ,  A61K2039/70 ,  C07K14/005 ,  C07K14/24 ,  C07K2319/02 ,  C07K2319/40 ,  C07K2319/74 ,  C12N15/1027 ,  C12N15/1034 ,  C12N15/1037 ,  C12N15/62 ,  C12N2710/16634 ,  C12N2730/10134 ,  C12N2740/16134 ,  C12N2760/12134 ,  C12N2770/24134 ,  C12N2770/24234 ,  C12N2770/36134 ,  Y02A50/472 ,  Y02A50/474

摘要： This invention is directed to antigen library immunization, which provides methods for obtaining antigens having improved properties for therapeutic and other uses. The methods are useful for obtaining improved antigens that can induce an immune response against pathogens, cancer, and other conditions, as well as antigens that are effective in modulating allergy, inflammatory and autoimmune diseases.

摘要翻译： 本发明涉及抗原文库免疫，其提供获得治疗和其它用途具有改进性质的抗原的方法。 该方法可用于获得可诱导针对病原体，癌症和其它病症的免疫应答的改良抗原以及有效调节过敏，炎症和自身免疫性疾病的抗原。

公开(公告)号：US06521453B1

公开(公告)日：2003-02-18

申请号：US09694863

申请日：2000-10-23

申请人： Andreas Crameri ,  Willem P. C. Stemmer ,  Jeremy Minshull ,  Steven H. Bass ,  Mark Welch ,  Jon E. Ness ,  Claes Gustafsson ,  Phillip A. Patten

发明人： Andreas Crameri ,  Willem P. C. Stemmer ,  Jeremy Minshull ,  Steven H. Bass ,  Mark Welch ,  Jon E. Ness ,  Claes Gustafsson ,  Phillip A. Patten

IPC分类号： C12N1500

CPC分类号： C12N15/11 ,  A61K39/00 ,  B01J2219/00689 ,  C07K14/005 ,  C07K14/505 ,  C07K14/535 ,  C12N9/16 ,  C12N15/1031 ,  C12N15/1034 ,  C12N2740/16122

摘要： Methods of recombining nucleic acids, including homologous nucleic acids, are provided. Families of gene shuffling oligonucleotides and their use in recombination procedures, as well as polymerase and ligase mediated recombination methods are also provided.

摘要翻译： 提供重组核酸的方法，包括同源核酸。 还提供了基因改组寡核苷酸家族及其在重组过程中的应用，以及聚合酶和连接酶介导的重组方法。

公开(公告)号：US06483011B1

公开(公告)日：2002-11-19

申请号：US09721540

申请日：2000-11-22

申请人： Willem P. C. Stemmer ,  Venkiteswaran Subramanian

发明人： Willem P. C. Stemmer ,  Venkiteswaran Subramanian

IPC分类号： A01H100

CPC分类号： C12Q1/48

摘要： The invention provides methods and compositions relating to sequence-shuffled variants of ADP-glucose pyrophosphorylase.

摘要翻译： 本发明提供了与ADP-葡萄糖焦磷酸化酶的序列洗牌变体相关的方法和组合物。

公开(公告)号：US06372497B1

公开(公告)日：2002-04-16

申请号：US09590774

申请日：2000-06-08

申请人： Willem P. C. Stemmer

发明人： Willem P. C. Stemmer

IPC分类号： C12N1500

CPC分类号： C40B40/02 ,  C07K14/43595 ,  C07K14/545 ,  C07K16/00 ,  C07K2317/565 ,  C07K2317/622 ,  C12N9/2471 ,  C12N9/86 ,  C12N15/1027 ,  C12N15/1034 ,  C12N15/1037 ,  C12N15/1058 ,  C12N15/52 ,  C12N15/64 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Y302/01023 ,  C12Y305/02006 ,  H01L39/228 ,  C12Q2525/179

摘要： A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.

公开(公告)号：US06297053B1

公开(公告)日：2001-10-02

申请号：US09501698

申请日：2000-02-10

申请人： Willem P. C. Stemmer

发明人： Willem P. C. Stemmer

IPC分类号： C12N1500

CPC分类号： C40B40/02 ,  C07K14/43595 ,  C07K14/545 ,  C07K16/00 ,  C07K2317/565 ,  C07K2317/622 ,  C12N9/2471 ,  C12N9/86 ,  C12N15/1027 ,  C12N15/1034 ,  C12N15/1037 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Y302/01023 ,  C12Y305/02006 ,  C40B40/08 ,  C40B50/06

摘要： A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.