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    Kinase sequences useful for developing compounds for the prevention and/or treatment of metabolic diseases and nucleotide sequences encoding such kinase sequences
    43.
    发明申请
    Kinase sequences useful for developing compounds for the prevention and/or treatment of metabolic diseases and nucleotide sequences encoding such kinase sequences 审中-公开
    用于开发用于预防和/或治疗代谢疾病的化合物的激酶序列和编码这种激酶序列的核苷酸序列

    公开(公告)号：US20060275767A1

    公开(公告)日：2006-12-07

    申请号：US10540634

    申请日：2003-12-19

    申请人： Gert Jules De Wilde

    发明人： Gert Jules De Wilde

    IPC分类号： C12Q1/68 ,  C07H21/04 ,  C12P21/06 ,  A61K39/395 ,  C12N9/12

    CPC分类号： C07K16/44 ,  C12N9/1205 ,  C12Q1/485 ,  G01N2500/04

    摘要： The present invention relates to nucleotide sequences that encode and may be used to express amino acid sequences that are useful in the identification and development of compounds with activity as pharmaceuticals, in particular of compounds for the prevention and treatment of metabolic diseases such as diabetes and obesity. The invention also relates to the amino acid sequences—such as proteins and polypeptides—that are encoded by, and that may be obtained by suitable expression of, the nucleotide sequences of the invention, particularly the amino acid sequences of JIK, PSK, TAO1 and Q9P2I6. The invention also relates to various uses and modulators of, and methods incorporating, the nucleotide and amino acid sequences of the invention.

    摘要翻译： 本发明涉及编码并可用于表达可用于鉴定和开发具有作为药物的化合物的氨基酸序列的核苷酸序列，特别是用于预防和治疗代谢性疾病如糖尿病和肥胖的化合物 。 本发明还涉及由本发明的核苷酸序列，特别是JIK，PSK，TAO1和（或）的氨基酸序列编码并可通过适当表达本发明的核苷酸序列而获得的氨基酸序列 - 例如蛋白质和多肽 - Q9P2I6。 本发明还涉及本发明的核苷酸和氨基酸序列的各种用途和调节剂以及结合本发明的核苷酸和氨基酸序列的方法。

    Characterisation of gene function using double stranded RNA inhibition
    45.
    发明申请
    Characterisation of gene function using double stranded RNA inhibition 有权
    使用双链RNA抑制表征基因功能

    公开(公告)号：US20040187170A1

    公开(公告)日：2004-09-23

    申请号：US10826522

    申请日：2004-04-16

    申请人： Devgen NV

    发明人： Geert Plaetinck ,  Christ Platteeuw ,  Katherine Mortier ,  Thierry Bogaert

    IPC分类号： A01K067/033 ,  C12N015/85

    CPC分类号： C12N15/1079 ,  C12N15/1093 ,  C12N15/8216 ,  C12N15/8218 ,  C12N15/8279 ,  C12N15/8285 ,  C12N15/86 ,  C12N2795/10243 ,  C12N2800/40 ,  C12N2830/002 ,  C12N2830/008 ,  C12N2830/42 ,  C12Q1/68 ,  Y02A40/164

    摘要： There is provided a method of identifying DNA responsible for conferring a particular phenotype in a cell which method comprises a) constructing a cDNA or genomic library of the DNA of said cell in a suitable vector in an orientation relative to a promoter(s) capable of initiating transcription of said cDNA or DNA to double stranded (ds) RNA upon binding of an appropriate transcription factor to said promoter(s), b) introducing said library into one or more of said cells comprising said transcription factor, and c) identifying and isolating a particular phenotype of said cell comprising said library and identifying the DNA or cDNA fragment from said library responsible for conferring said phenotype. Using this technique it is also possible to assign function to a known DNA sequence by a) identifying a homologue(s) of said DNA sequence in a cell, b) isolating the relevant DNA homologue(s) or a fragment thereof from said cell, c) cloning said homologue or fragment thereof into an appropriate vector in an orientation relative to a suitable promoter(s) capable of initiating transcription of dsRNA from said DNA homologue or fragment upon binding of an appropriate transcription factor to said promoter(s) and d) introducing said vector into said cell from step a) comprising said transcription factor.

    摘要翻译： 提供了鉴定负责赋予细胞中特定表型的DNA的方法，该方法包括a）以合适的载体构建所述细胞的DNA的cDNA或基因组文库，其方向相对于能够 在将合适的转录因子结合到所述启动子时，将所述cDNA或DNA转录到双链（ds）RNA; b）将所述文库引入到一个或多个包含所述转录因子的细胞中，和c）鉴定和 分离包含所述文库的所述细胞的特定表型，并从负责赋予所述表型的所述文库鉴定DNA或cDNA片段。 使用这种技术，还可以通过以下方式将功能分配给已知的DNA序列：a）鉴定细胞中所述DNA序列的同源物，b）从所述细胞中分离相关的DNA同系物或其片段， c）相对于合适的启动子，将所述同源物或片段相对于适当的启动子克隆到适当的载体中，所述启动子能够在合适的转录因子与所述启动子结合时从所述DNA同源物或片段启动dsRNA的转录，并且d ）将所述载体从包含所述转录因子的步骤a）引入所述细胞。