公开(公告)号：US20080139471A1

公开(公告)日：2008-06-12

申请号：US11854654

申请日：2007-09-13

申请人： Tapas Das Gupta ,  Ananda Chakrabarty

发明人： Tapas Das Gupta ,  Ananda Chakrabarty

IPC分类号： A61K38/16 ,  C07K14/195 ,  C12Q1/02 ,  A61P35/04 ,  C12N15/00 ,  C07K2/00

CPC分类号： A61K38/16 ,  A61K38/00 ,  A61K38/164 ,  A61K38/168 ,  A61K45/06 ,  C07K14/195 ,  C07K14/21 ,  C07K14/22 ,  C07K14/225 ,  C07K14/235 ,  C07K14/28 ,  C07K14/35 ,  C07K14/415 ,  G01N33/5011 ,  G01N33/5017 ,  G01N33/569 ,  G01N2510/00

摘要： The present invention relates to compositions comprising peptides that may be variants, derivatives and structural equivalents of cupredoxins that inhibit the development of premalignant lesions in mammalian cells, tissues and animals. Specifically, these compositions may comprise azurin from Pseudomonas aeruginosa, and/or the 50-77 residue region of azurin (p28). The present invention further relates to compositions that may comprise cupredoxin(s), and/or variants, derivatives or structural equivalents of cupredoxins, that retain the ability to inhibit the development of premalignant lesions in mammalian cells, tissues or animals. These compositions may be peptides or pharmaceutical compositions, among others. The compositions of the invention may be used to prevent the development of premalignant lesions in mammalian cells, tissues and animals, and thus prevent cancer.

摘要翻译： 本发明涉及包含肽的组合物，其可以是抑制哺乳动物细胞，组织和动物中恶化前病变发展的铜氧还蛋白的变体，衍生物和结构等同物。 具体地，这些组合物可以包含来自绿脓假单胞菌的吖啶素和/或吖啶的50-77残基区域（p28）。 本发明还涉及可包含铜氧还蛋白的组合物和/或氧还蛋白的变体，衍生物或结构等同物，其保留抑制哺乳动物细胞，组织或动物中恶化前病变发展的能力。 这些组合物可以是肽或药物组合物等。 本发明的组合物可用于预防哺乳动物细胞，组织和动物中癌前病变的发展，从而预防癌症。

公开(公告)号：US20080108129A1

公开(公告)日：2008-05-08

申请号：US11813273

申请日：2005-12-29

申请人： Jacob Pitcovski ,  Jacob Vaya

发明人： Jacob Pitcovski ,  Jacob Vaya

IPC分类号： C12N7/06 ,  C07K1/00 ,  C07K16/18

CPC分类号： C07K16/00 ,  C07K14/245 ,  C07K14/28 ,  C07K16/081 ,  C07K2317/11 ,  C07K2317/12 ,  C07K2317/23 ,  C07K2317/41 ,  C12N7/00 ,  C12N2710/10222

摘要： Methods for obtaining modified proteins or virus with an intact native binding site and decreased antigenicity and modified proteins or virus obtainable by said methods are provided. The methods of protein or virus modification comprise masking with non-immunogenic molecules the protein or the virus surface, except for the protein or the virus binding site. Examples of modified proteins or virus that can be modified in accordance to the methods include polyclonal or monoclonal antibodies, modified replication-defective virus, hormones, and enterotoxins.

摘要翻译： 提供了通过完整的天然结合位点获得修饰的蛋白质或病毒并降低抗原性的方法以及通过所述方法获得的修饰的蛋白质或病毒。 蛋白质或病毒修饰的方法包括除了蛋白质或病毒结合位点之外的蛋白质或病毒表面的非免疫原性分子的掩蔽。 可以根据方法修饰的修饰的蛋白质或病毒的实例包括多克隆或单克隆抗体，修饰的复制缺陷病毒，激素和肠毒素。

公开(公告)号：US20080107691A1

公开(公告)日：2008-05-08

申请号：US11753311

申请日：2007-05-24

申请人： Manickam RAVICHANDRAN ,  Syed Atif ALI ,  Nur Haslindawaty Abdul Rashid ,  Lalitha PATTABIRAMAN ,  Zainul Fadziruddin ZAINUDDIN

发明人： Manickam RAVICHANDRAN ,  Syed Atif ALI ,  Nur Haslindawaty Abdul Rashid ,  Lalitha PATTABIRAMAN ,  Zainul Fadziruddin ZAINUDDIN

IPC分类号： A61K39/106 ,  C12N15/63 ,  A61P31/04 ,  C12N1/21

CPC分类号： A61K39/107 ,  A61K2039/522 ,  A61K2039/542 ,  C07K14/28 ,  Y02A50/472

摘要： Metabolic auxotroph of Vibrio cholerae 0139 synonym Bengal which has a mutation in its hem A gene and which is not capable of synthesizing aminolevulinic acid (ALA) de novo and which is obtained from a parent strain originally isolated from a patient's coproculture having all the identifying characteristics of Vibrio cholerae 0139 synonym Bengal is described. In this strain the hem A gene is mutated by inserting a kanamycin resistant gene cassette. Another metabolic auxotroph of Vibrio cholerae 01 El Tor where the hem A gene is mutated by a frame shift mutation is disclosed. Methods of producing the strains are disclosed.

摘要翻译： 霍乱弧菌的代谢营养缺陷型0139同义词孟加拉，其在其下摆A基因中具有突变，并且不能从头合成氨基乙酰丙酸（ALA），并且其从最初从具有所有鉴定特征的患者的共培养物中分离的亲本菌株获得 Vibrio cholerae 0139同义词孟加拉描述。 在该菌株中，通过插入卡那霉素抗性基因盒突变下摆A基因。 披露了通过框架移位突变突变的下摆A基因的霍乱弧菌01 El Tor的另一代谢营养缺陷型。 公开了生产菌株的方法。

公开(公告)号：US20080044872A1

公开(公告)日：2008-02-21

申请号：US11778611

申请日：2007-07-16

申请人： James Metz ,  Craig Weaver ,  William Barclay ,  James Flatt

发明人： James Metz ,  Craig Weaver ,  William Barclay ,  James Flatt

IPC分类号： C12P7/64 ,  C07H21/00 ,  C12N1/00 ,  C12N1/21 ,  C12N5/10

CPC分类号： C12N15/52 ,  A23D7/001 ,  A23L33/115 ,  A23L33/135 ,  C07K14/28 ,  C12P7/6427 ,  C12P7/6472

摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

公开(公告)号：US20080038795A1

公开(公告)日：2008-02-14

申请号：US11778590

申请日：2007-07-16

申请人： James Metz ,  Craig Weaver ,  William Barclay ,  James Flatt

发明人： James Metz ,  Craig Weaver ,  William Barclay ,  James Flatt

IPC分类号： C12P7/64 ,  C07H21/00 ,  C12N1/00 ,  C12N1/12 ,  C12N1/21 ,  C12N5/04

CPC分类号： C12N15/52 ,  A23D7/001 ,  A23L33/115 ,  A23L33/135 ,  C07K14/28 ,  C12P7/6427 ,  C12P7/6472

摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

公开(公告)号：US20080038794A1

公开(公告)日：2008-02-14

申请号：US11778582

申请日：2007-07-16

申请人： James Metz ,  Craig Weaver ,  William Barclay ,  James Flatt

发明人： James Metz ,  Craig Weaver ,  William Barclay ,  James Flatt

IPC分类号： C12P7/64 ,  C07H21/00 ,  C12N1/13 ,  C12N1/21 ,  C12N5/04

CPC分类号： C12N15/52 ,  A23D7/001 ,  A23L33/115 ,  A23L33/135 ,  C07K14/28 ,  C12P7/6427 ,  C12P7/6472

摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

公开(公告)号：US20080038793A1

公开(公告)日：2008-02-14

申请号：US11778564

申请日：2007-07-16

申请人： James Metz ,  Craig Weaver ,  William Barclay ,  James Flatt

发明人： James Metz ,  Craig Weaver ,  William Barclay ,  James Flatt

IPC分类号： C12P7/64 ,  C12N1/00 ,  C12N1/12 ,  C12N1/21

CPC分类号： C12N15/52 ,  A23D7/001 ,  A23L33/115 ,  A23L33/135 ,  C07K14/28 ,  C12P7/6427 ,  C12P7/6472

摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

公开(公告)号：US20080038790A1

公开(公告)日：2008-02-14

申请号：US11689605

申请日：2007-03-22

申请人： James Metz ,  James Flatt ,  Jerry Kuner ,  William Barclay

发明人： James Metz ,  James Flatt ,  Jerry Kuner ,  William Barclay

IPC分类号： C12P7/64 ,  C07H21/00 ,  C12N1/00 ,  C12N1/12 ,  C12N1/21 ,  C12N5/04

CPC分类号： C12N15/52 ,  C07K14/28 ,  C07K14/405 ,  C12N15/8247 ,  C12N15/8273 ,  C12P7/625 ,  C12P7/6427 ,  C12P7/6472

摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

公开(公告)号：US20080026440A1

公开(公告)日：2008-01-31

申请号：US11689608

申请日：2007-03-22

申请人： James Metz ,  James Flatt ,  Jerry Kuner ,  William Barclay

发明人： James Metz ,  James Flatt ,  Jerry Kuner ,  William Barclay

IPC分类号： C12P7/64 ,  C07H21/00 ,  C12N1/00 ,  C12N1/21 ,  C12N5/04

CPC分类号： C12N15/52 ,  C07K14/28 ,  C07K14/405 ,  C12N15/8247 ,  C12N15/8273 ,  C12P7/625 ,  C12P7/6427 ,  C12P7/6472

摘要： The invention generally relates to polyunsaturated fatty acid (PUFA) polyketide synthase (PKS) systems isolated from or derived from non-bacterial organisms, to homologues thereof, to isolated nucleic acid molecules and recombinant nucleic acid molecules encoding biologically active domains of such a PUFA PKS system, to genetically modified organisms comprising PUFA PKS systems, to methods of making and using such systems for the production of bioactive molecules of interest, and to novel methods for identifying new bacterial and non-bacterial microorganisms having such a PUFA PKS system.

公开(公告)号：US20070264715A1

公开(公告)日：2007-11-15

申请号：US10577119

申请日：2004-11-10

申请人： Gary Robinson ,  Sue Cook

发明人： Gary Robinson ,  Sue Cook

IPC分类号： C12N1/38 ,  C12N1/20 ,  C12N15/74 ,  C12N9/48

CPC分类号： C07K14/28 ,  A01N61/00 ,  A01N63/02 ,  A61K38/06 ,  A61K38/465 ,  A61K38/55 ,  C12Y301/13004 ,  Y02A50/475

摘要： The method relates to a method of modulating quorum sensing in bacteria. Quorum sensing is inhibited using peptide hydrolases. This inhibition is used to prevent biofilm formation or to break down established biofilms and may also be used to downregulate the production of virulence determinants by pathogenic bacteria. The invention also relates to the use of peptide hydrolase inhibitors for the upregulation of quorum sensing in bacteria, resulting in the overproduction of proteins and the use of this system as an expression system.

摘要翻译： 该方法涉及一种调节细菌群体感应的方法。 使用肽水解酶抑制法定感应。 这种抑制用于防止生物膜形成或破坏已建立的生物膜，并且还可用于下调致病细菌产生毒力决定簇。 本发明还涉及肽水解酶抑制剂在细菌中上调群体感应的用途，导致蛋白质的过量产生以及该系统作为表达系统的用途。