公开(公告)号：US20050250178A1

公开(公告)日：2005-11-10

申请号：US10614599

申请日：2003-07-07

Applicant: David Andrew ,  David Lewin ,  Diane Pennica ,  Luca Rastelli ,  Bruce Tallion

Inventor： David Andrew ,  David Lewin ,  Diane Pennica ,  Luca Rastelli ,  Bruce Tallion

IPC: A61K38/00 ,  C07K14/52 ,  C12N1/21 ,  C12N15/19 ,  C07H21/04 ,  C07K14/705 ,  C07K16/28

CPC classification number: C07K14/52 ,  A61K38/00

Abstract: The present invention provides novel polypeptides, termed FCTRX polypeptides, as well as polynucleotides encoding FCTRX polypeptides and antibodies that immunospecifically bind to an FCTRX or a derivative, variant, mutant, or fragment of an FCTRX polypeptide, polynucleotide or antibody. The invention additionally provides methods in which the FCTRX polypeptide, polynucleotide and antibody are used in detection and treatment of a broad range of pathological states, as well as to other uses.

Abstract translation: 本发明提供了称为FCTRX多肽的新型多肽，以及编码FCTRX多肽的多核苷酸和免疫特异性结合FCTRX或FCTRX多肽，多核苷酸或抗体的衍生物，变体，突变体或片段的抗体。 本发明还提供了FCTRX多肽，多核苷酸和抗体用于检测和治疗广泛的病理状态以及其它用途的方法。

公开(公告)号：US20050159373A1

公开(公告)日：2005-07-21

申请号：US10422335

申请日：2003-04-24

Applicant: Diane Pennica ,  Luca Rastelli

Inventor： Diane Pennica ,  Luca Rastelli

IPC: A01K67/027 ,  A61K31/7088 ,  A61K38/00 ,  A61K39/395 ,  A61K45/00 ,  A61K48/00 ,  A61P9/10 ,  A61P35/00 ,  A61P43/00 ,  C07K14/00 ,  C07K14/47 ,  C07K16/18 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N15/09 ,  C12N15/12 ,  C12Q1/02 ,  C12Q1/68

CPC classification number: C07K14/47 ,  A01K2217/05 ,  A01K2217/075 ,  A61K48/00

Abstract: An isolated polypeptide comprising an amino acid sequence having at least 80% sequence identity to the sequence SEQ ID NOS:2, 4, 6 or 8, polynucleotides encoding these peptides, and antibodies to the polypeptides are useful in treating cancers.

Abstract translation: 包含与序列SEQ ID NO：2,4,6或8具有至少80％序列同一性的氨基酸序列的分离的多肽，编码这些肽的多核苷酸和针对该多肽的抗体可用于治疗癌症。

公开(公告)号：US20050042699A1

公开(公告)日：2005-02-24

申请号：US10895545

申请日：2004-07-21

Applicant: David Botstein ,  Audrey Goddard ,  David Lawrence ,  Diane Pennica ,  Margaret Roy ,  William Wood

Inventor： David Botstein ,  Audrey Goddard ,  David Lawrence ,  Diane Pennica ,  Margaret Roy ,  William Wood

IPC: A61K38/00 ,  C07K14/47 ,  C07K14/52 ,  C07K16/18 ,  C07K16/24 ,  C12Q1/68 ,  A61K39/395 ,  G01N33/574

CPC classification number: C12Q1/6886 ,  A01K2217/05 ,  A61K38/00 ,  C07K14/47 ,  C07K14/4703 ,  C07K14/52 ,  C07K16/18 ,  C07K16/24 ,  C07K2317/24 ,  C07K2319/00 ,  C07K2319/02 ,  C12Q2600/136 ,  G01N33/57484 ,  G01N2333/52 ,  G01N2500/00

Abstract: The invention concerns composititions and methods for the diagnosis and treatment of neoplastic cell growth and proliferation in mammals, including humans. The invention is based on the identification of cardiotrophin-1 gene amplified in the genome of tumor cells. Such gene amplification is expected to be associated with the overexpression of the gene product and contribute to tumorigenesis. Acordingly, the cardiotrophin-1 polypeptide encoded by the amplified gene is believed to be a useful target for the diagnosis and/or treatment (including prevention) of certain cancers, and may act as a predictor of the prognosis of tumor treatment.

Abstract translation: 本发明涉及用于诊断和治疗哺乳动物（包括人）中的肿瘤细胞生长和增殖的复合和方法。 本发明基于在肿瘤细胞基因组中扩增的心肌营养因子-1基因的鉴定。 预期这种基因扩增与基因产物的过表达有关，并有助于肿瘤发生。 因此，由扩增的基因编码的心肌营养因子-1多肽被认为是某些癌症的诊断和/或治疗（包括预防）的有用靶标，并且可以作为肿瘤治疗预后的预测因子。

公开(公告)号：US5763253A

公开(公告)日：1998-06-09

申请号：US474160

申请日：1995-06-06

Applicant: David V. Goeddel ,  William J. Kohr ,  Diane Pennica ,  Gordon A. Vehar

Inventor： David V. Goeddel ,  William J. Kohr ,  Diane Pennica ,  Gordon A. Vehar

IPC: C12N15/09 ,  A61K20060101 ,  A61K35/12 ,  A61K35/74 ,  A61K38/00 ,  A61K38/46 ,  C07H21/02 ,  C07H21/04 ,  C07K14/00 ,  C12N20060101 ,  C12N1/00 ,  C12N1/16 ,  C12N1/20 ,  C12N1/21 ,  C12N5/00 ,  C12N5/10 ,  C12N7/00 ,  C12N9/50 ,  C12N9/52 ,  C12N9/64 ,  C12N9/72 ,  C12N15/00 ,  C12N15/12 ,  C12N15/58 ,  C12N15/70 ,  C12N15/85 ,  C12P19/34 ,  C12P21/00 ,  C12R1/125 ,  C12R1/19 ,  C12R1/42 ,  C12R1/43 ,  C12R1/865 ,  C12R1/91 ,  G01N20060101 ,  C12N15/55

CPC classification number: C12N9/6459 ,  C12Y304/21069 ,  A61K38/00

Abstract: Tissue plasminogen activator (t-PA) derivatives are produced in useful quantities using recombinant DNA techniques. Specific derivatives include amino acid deletion derivatives and amino acid substitution derivatives. A deletion derivative lacking the N-terminal first 68 amino acids is specifically exemplified having requisite t-PA characteristics. The invention disclosed thus enables the production of t-PA derivatives via recombinant means. Methods, expression vehicles and various host cells useful in the production of said t-PA derivatives are also disclosed.

Abstract translation: 组织纤溶酶原激活物（t-PA）衍生物使用重组DNA技术以有用的量产生。 具体的衍生物包括氨基酸缺失衍生物和氨基酸取代衍生物。 具有必需的t-PA特征的具体示例是缺少N-末端第68个氨基酸的缺失衍生物。 因此，所公开的本发明能够通过重组方式生产t-PA衍生物。 还公开了可用于生产所述t-PA衍生物的方法，表达载体和各种宿主细胞。

公开(公告)号：US5753486A

公开(公告)日：1998-05-19

申请号：US472549

申请日：1995-06-06

Applicant: David V. Goeddel ,  William J. Kohr ,  Diane Pennica ,  Gordon A. Vehar

Inventor： David V. Goeddel ,  William J. Kohr ,  Diane Pennica ,  Gordon A. Vehar

IPC: A61K38/00 ,  C12N1/21 ,  C12N9/06 ,  C12N9/72 ,  C12N15/58 ,  C12N15/71 ,  C12P20060101 ,  C12N9/50 ,  A61K38/49 ,  C12N9/64 ,  C12N15/55

CPC classification number: C12N15/71 ,  C12N9/003 ,  C12N9/6459 ,  C12Y304/21069 ,  A61K38/00

Abstract: A human tissue plasminogen activator (t-PA) is produced in useful quantities using recombinant DNA techniques. The invention disclosed thus enables the production of t-PA free of contaminants with which it is ordinarily associated in its native cellular environment. Methods, expression vehicles and various host cells useful in its production are also disclosed.

Abstract translation: 使用重组DNA技术以有用的量产生人组织纤溶酶原激活物（t-PA）。 因此，所公开的发明使得能够生产没有其天然细胞环境中通常相关的污染物的t-PA。 还公开了可用于其生产的方法，表达载体和各种宿主细胞。

公开(公告)号：US5702938A

公开(公告)日：1997-12-30

申请号：US468974

申请日：1995-06-06

Applicant: David V. Goeddel ,  William J. Kohr ,  Diane Pennica ,  Gordon A. Vehar

Inventor： David V. Goeddel ,  William J. Kohr ,  Diane Pennica ,  Gordon A. Vehar

IPC: A61K38/00 ,  C12N1/21 ,  C12N9/06 ,  C12N9/72 ,  C12N15/58 ,  C12N15/71 ,  C12P20060101 ,  C12N9/50

CPC classification number: C12N15/71 ,  C12N9/003 ,  C12N9/6459 ,  C12Y304/21069 ,  A61K38/00

Abstract: Human tissue plasminogen activator (t-PA) is produced in useful quantities using recombinant DNA techniques. The invention disclosed thus enables the production of t-PA free of contaminants with which it is ordinarily associated in its native cellular environment. Methods, expression vehicles and various host cells useful in its production are also disclosed.

Abstract translation: 使用重组DNA技术以有用的量产生人组织纤溶酶原激活物（t-PA）。 因此，所公开的发明使得能够生产没有其天然细胞环境中通常相关的污染物的t-PA。 还公开了可用于其生产的方法，表达载体和各种宿主细胞。

公开(公告)号：US5627073A

公开(公告)日：1997-05-06

申请号：US443129

申请日：1995-05-17

Applicant: Joffre Baker ,  Kenneth Chien ,  Kathleen King ,  Diane Pennica ,  William Wood

Inventor： Joffre Baker ,  Kenneth Chien ,  Kathleen King ,  Diane Pennica ,  William Wood

IPC: G01N33/53 ,  A61K38/00 ,  C07K14/52 ,  C07K16/24 ,  C12N5/10 ,  C12N15/02 ,  C12N15/09 ,  C12N15/19 ,  C12P21/02 ,  C12P21/08 ,  C12Q1/02 ,  C12Q1/68 ,  C12R1/91 ,  G01N33/68 ,  C12N5/18 ,  C12N5/22

CPC classification number: C12Q1/025 ,  C07K14/52 ,  G01N33/6872 ,  A01K2217/05 ,  A61K38/00 ,  G01N2500/00 ,  Y10S930/14

Abstract: Isolated CHF (also referred to cardiac hypertrophy factor or cardiotrophin-1), isolated DNA encoding CHF, hybridomas and cell lines producing antibodies to CHF, and recombinant or synthetic methods of preparing CHF are disclosed. These CHF molecules are shown to influence hypertrophic activity and neurological activity. Accordingly, these compounds or their antagonists may be used for treatment of heart failure, arrhythmic disorders, inotropic disorders, and neurological disorders.

Abstract translation: 分离的CHF（也称为心脏肥大因子或心肌营养因子-1），编码CHF的分离的DNA，产生CHF抗体的杂交瘤和细胞系，以及制备CHF的重组或合成方法。 显示这些CHF分子影响增生活性和神经活性。 因此，这些化合物或其拮抗剂可用于治疗心力衰竭，心律不齐症，肌力障碍和神经障碍。

公开(公告)号：US5587159A

公开(公告)日：1996-12-24

申请号：US264134

申请日：1994-06-21

Applicant: David V. Goeddel ,  William J. Kohr ,  Diane Pennica ,  Gordon A. Vehar

Inventor： David V. Goeddel ,  William J. Kohr ,  Diane Pennica ,  Gordon A. Vehar

IPC: A61K38/00 ,  C12N1/21 ,  C12N9/06 ,  C12N9/72 ,  C12N15/58 ,  C12N15/71 ,  C12P20060101 ,  A61K38/49 ,  C12N9/64 ,  C12N15/15 ,  C12N15/70

CPC classification number: C12N15/71 ,  C12N9/003 ,  C12N9/6459 ,  C12Y304/21069 ,  A61K38/00

Abstract: Human tissue plasminogen activator (t-PA) is produced in useful quantities using recombinant DNA techniques. The invention disclosed thus enables the production of t-PA free of contaminants with which it is ordinarily associated in its native cellular environment. Methods, expression vehicles and various host cells useful in its production are also disclosed.

Abstract translation: 使用重组DNA技术以有用的量产生人组织纤溶酶原激活物（t-PA）。 因此，所公开的发明使得能够生产没有其天然细胞环境中通常相关的污染物的t-PA。 还公开了可用于其生产的方法，表达载体和各种宿主细胞。

公开(公告)号：US5185259A

公开(公告)日：1993-02-09

申请号：US489855

申请日：1990-03-02

Applicant: David V. Goeddel ,  William J. Kohr ,  Diane Pennica ,  Gordon A. Vehar

Inventor： David V. Goeddel ,  William J. Kohr ,  Diane Pennica ,  Gordon A. Vehar

IPC: A61K38/00 ,  C12N9/06 ,  C12N9/72 ,  C12N15/58 ,  C12N15/71

CPC classification number: C12N9/003 ,  C12N15/71 ,  C12N9/6459 ,  C12Y304/21069 ,  A61K38/00

Abstract: Human tissue plasminogen activator (t-PA) is produced in useful quantities using recombinant DNA techniques. The invention disclosed thus enables the production of t-PA free of contaminants with which it is ordinarily associated in its native cellular environment. Methods, expression vehicles and various host cells useful in its production are also disclosed. A truncated human tissue plasminogen activator consisting essentially of amino acids 69-527 is produced using recombinant DNA techniques.

Abstract translation: 使用重组DNA技术以有用的量产生人组织纤溶酶原激活物（t-PA）。 因此，所公开的发明使得能够生产没有其天然细胞环境中通常相关的污染物的t-PA。 还公开了可用于其生产的方法，表达载体和各种宿主细胞。 使用重组DNA技术产生基本上由氨基酸69-527组成的截短的人组织纤溶酶原激活物。

公开(公告)号：US5011795A

公开(公告)日：1991-04-30

申请号：US499201

申请日：1990-03-22

Applicant: Arthur D. Levinson ,  Diane Pennica ,  William J. Kohr ,  Gordon A. Vehar ,  David V. Goeddel ,  Elizabeth M. Yelverton ,  Christian C. Simonsen

Inventor： Arthur D. Levinson ,  Diane Pennica ,  William J. Kohr ,  Gordon A. Vehar ,  David V. Goeddel ,  Elizabeth M. Yelverton ,  Christian C. Simonsen

IPC: C12N9/06 ,  C12N9/72 ,  C12N15/58 ,  C12N15/85

CPC classification number: C12N9/0028 ,  C12N15/85 ,  C12N9/6459 ,  C12Y304/21069 ,  C12N2800/108 ,  C12N2840/20

Abstract: A method for producing tissue plasminogen activator (t-PA) in eukaryotic host cells is disclosed. Enhanced levels of t-PA production are obtained by co-amplification of the t-PA gene through treatment of cultures transformed with mutant or wild type DHFR with methotrexate.

Abstract translation: 公开了一种在真核宿主细胞中产生组织纤溶酶原激活物（t-PA）的方法。 通过用甲氨蝶呤处理用突变体或野生型DHFR转化的培养物共同扩增t-PA基因，获得增强的t-PA产生水平。