公开(公告)号：US20160362681A1

公开(公告)日：2016-12-15

申请号：US15156214

申请日：2016-05-16

Applicant: Gigagen, Inc.

Inventor： David Scott Johnson ,  Adam Adler ,  Rena Mizrahi

IPC: C12N15/10 ,  C07K16/00

CPC classification number: C12N15/1037 ,  C07K14/7051 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/622 ,  C12N15/1075 ,  C12N15/1093 ,  C12Q1/6876 ,  C40B50/06

Abstract: Disclosed herein are methods and compositions for generating a repertoire of recombinant fusion polypeptides from immune cells, and uses thereof.

公开(公告)号：US20160362470A1

公开(公告)日：2016-12-15

申请号：US15159705

申请日：2016-05-19

Applicant: Gigagen, Inc.

Inventor： David Scott Johnson ,  Adam Adler ,  Rena Mizrahi

IPC: C07K14/725 ,  C12N15/10

CPC classification number: C12N15/1037 ,  C07K14/7051 ,  C07K16/00 ,  C07K2317/14 ,  C07K2317/622 ,  C12N15/1075 ,  C12N15/1093 ,  C12Q1/6876 ,  C40B50/06

Abstract: Disclosed herein are methods and compositions for generating a repertoire of recombinant fusion polypeptides from immune cells, and uses thereof.

公开(公告)号：US20160326583A1

公开(公告)日：2016-11-10

申请号：US15159674

申请日：2016-05-19

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Everett Hurteau Meyer

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C07K16/00 ,  C07K2317/622 ,  C12N15/1006 ,  C12N15/1065 ,  C12N15/1075 ,  C12Q1/6837 ,  C12Q1/6846 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q1/6888 ,  C12Q2600/156 ,  C40B50/06 ,  C12Q2525/161 ,  C12Q2525/307 ,  C12Q2563/159

Abstract: Methods and systems are provided for massively parallel genetic analysis of single cells in emulsion droplets or reaction containers. Genetic loci of interest are targeted in a single cell using a set of probes, and a fusion complex is formed by molecular linkage and amplification techniques. Methods are provided for high-throughput, massively parallel analysis of the fusion complex in a single cell in a population of at least 10,000 cells. Also provided are methods for tracing genetic information back to a cell using barcode sequences.

公开(公告)号：US20160264924A1

公开(公告)日：2016-09-15

申请号：US15160671

申请日：2016-05-20

Applicant: GigaGen, Inc.

Inventor： David Scott Johnson ,  Gary Withey ,  Philip Homewood ,  Timothy Atkins

IPC: C12N1/06 ,  B01L3/00

CPC classification number: C12N1/06 ,  B01F3/08 ,  B01F13/0071 ,  B01F13/0094 ,  B01F2215/0422 ,  B01F2215/0431 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502784 ,  B01L2200/0647 ,  B01L2300/0867 ,  B01L2300/18 ,  B81B7/00 ,  G01N35/08

Abstract: Methods and systems are provided for merging a droplet with a volume of fluid in a microfluidic system. In particular, the methods of the invention use a microfluidic structure designed to merge a fluid with a droplet in order to dilute, add volume, or add selected reagents, biological materials, or synthetic materials to a droplet. Also provided are related systems and methods for cell lysis.

公开(公告)号：US20150167078A1

公开(公告)日：2015-06-18

申请号：US14568377

申请日：2014-12-12

Applicant: Gigagen, Inc.

Inventor： David Scott Johnson ,  Andrea Loehr

IPC: C12Q1/68

CPC classification number: C12Q1/6881 ,  C07K16/00 ,  C12Q2600/112 ,  C12Q2600/16

Abstract: A method is provided for discovering antibodies with desired properties. In one aspect of this invention, oligoclonal single chain repertoires are determined in a target tissue. Single cells from blood are then isolated and sequenced to determine the heavy and light chain pairing in each of thousands of cells. The oligoclonal single chain repertoire is then used to computationally select candidate paired chain antibodies to which the target tissue is antigenic. In another aspect of the invention, oligoclonal heavy and light chain repertoires are determined separately in a target tissue. A list of candidate fully paired antibodies are then identified computationally by an all-by-all pairing of the oligoclonal heavy chain repertoire with the oligoclonal light chain repertoire. This invention has practical application in many biological and medical specialties where antibodies are useful, such as therapeutics, diagnostics, molecular analysis, oncology, transplantation, and infectious disease.

Abstract translation: 提供了一种用于发现具有所需性质的抗体的方法。 在本发明的一个方面，在目标组织中测定寡克隆单链所有组成成分。 然后将来自血液的单细胞分离并测序以确定每千个细胞中的重链和轻链配对。 然后使用寡克隆单链谱来计算选择目标组织具有抗原性的候选配对链抗体。 在本发明的另一方面，在目标组织中单独测定寡克隆重链和轻链所有组成成分。 然后通过将寡克隆重链全序列与寡克隆轻链全部配对进行计算，鉴定候选完全配对抗体的列表。 本发明在诸如治疗学，诊断学，分子分析，肿瘤学，移植和感染性疾病之类的抗体有用的许多生物和医学专业中具有实际应用。