公开(公告)号：US12038405B2

公开(公告)日：2024-07-16

申请号：US18324346

申请日：2023-05-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Mark Milgrew ,  Jonathan Rothberg ,  James Bustillo

IPC: G01N27/414 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/27

CPC classification number: G01N27/4145 ,  C12Q1/6869 ,  C12Q1/6874 ,  G01N27/27 ,  G01N27/414 ,  Y10T29/49002

Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

公开(公告)号：US11499938B2

公开(公告)日：2022-11-15

申请号：US17070142

申请日：2020-10-14

Applicant: Life Technologies Corporation

Inventor： Jonathan M. Rothberg ,  Keith G Fife ,  Jordan Owens ,  James Bustillo

IPC: G01N27/414 ,  C12Q1/6874 ,  H01L29/66

Abstract: In one embodiment, a device is described. The device includes a material defining a reaction region. The device also includes a plurality of chemically-sensitive field effect transistors have a common floating gate in communication with the reaction region. The device also includes a circuit to obtain respective output signals from the chemically-sensitive field effect transistors indicating an analyte within the reaction region.

公开(公告)号：US11231388B2

公开(公告)日：2022-01-25

申请号：US16544106

申请日：2019-08-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James Li ,  Jordan Owens ,  James Bustillo

IPC: G01N27/414 ,  G01N27/12

Abstract: In one embodiment, a chemical sensor is described. The chemical sensor includes a chemically-sensitive field effect transistor including a floating gate conductor having an upper surface, a first opening extending through a first material and through a portion of a second material located on the first material and a second opening extending from the bottom of the first opening to the top of a liner layer located on the upper surface of the floating gate conductor.

公开(公告)号：US20210047686A1

公开(公告)日：2021-02-18

申请号：US16882414

申请日：2020-05-22

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan M. Rothberg ,  Wolfgang Hinz ,  Kim L. Johnson ,  James Bustillo ,  John Leamon ,  Jonathan Schultz

IPC: C12Q1/6874 ,  G01N27/414 ,  B01L3/00 ,  C12Q1/6869

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.

公开(公告)号：US10816504B2

公开(公告)日：2020-10-27

申请号：US16663052

申请日：2019-10-24

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan M. Rothberg ,  Keith G. Fife ,  James Bustillo ,  Jordan Owens

IPC: G01N27/414 ,  H01L29/66 ,  C12Q1/6874

Abstract: In one embodiment, a device is described. The device includes a material defining a reaction region. The device also includes a plurality of chemically-sensitive field effect transistors have a common floating gate in communication with the reaction region. The device also includes a circuit to obtain respective output signals from the chemically-sensitive field effect transistors indicating an analyte within the reaction region.

公开(公告)号：US10605770B2

公开(公告)日：2020-03-31

申请号：US15831141

申请日：2017-12-04

Applicant: Life Technologies Corporation

Inventor： Wolfgang Hinz ,  John Matthew Mauro ,  Shifeng Li ,  James Bustillo

IPC: G01N1/00 ,  G01N27/414 ,  G01N27/26 ,  C12Q1/6874 ,  H01L29/78

Abstract: The described embodiments may provide a method of fabricating a chemical detection device. The method may comprise forming a microwell above a CMOS device. The microwell may comprise a bottom surface and sidewalls. The method may further comprise applying a first chemical to be selectively attached to the bottom surface of the microwell, forming a metal oxide layer on the sidewalls of the microwell, and applying a second chemical to be selectively attached to the sidewalls of the microwell. The second chemical may lack an affinity to the first chemical.

公开(公告)号：US10481124B2

公开(公告)日：2019-11-19

申请号：US15818718

申请日：2017-11-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Keith G. Fife ,  Jordan Owens ,  Shifeng Li ,  James Bustillo

IPC: G01N27/414 ,  B01L3/00

Abstract: In one implementation, a chemical device is described. The sensor includes a chemically-sensitive field effect transistor including a floating gate structure having a plurality of floating gate conductors electrically coupled to one another. A conductive element overlies and is in communication with an uppermost floating gate conductor in the plurality of floating gate conductors. The conductive element is wider and thinner than the uppermost floating gate conductor. A dielectric material defines an opening extending to an upper surface of the conductive element.

公开(公告)号：US10436742B2

公开(公告)日：2019-10-08

申请号：US15826627

申请日：2017-11-29

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shifeng Li ,  James Bustillo

IPC: G01N27/414

Abstract: In one implementation, a method for manufacturing a chemical detection device is described. The method includes forming a chemical sensor having a sensing surface. A dielectric material is deposited on the sensing surface. A first etch process is performed to partially etch the dielectric material to define an opening over the sensing surface and leave remaining dielectric material on the sensing surface. An etch protect material is formed on a sidewall of the opening. A second etch process is then performed to selectively etch the remaining dielectric material using the etch protect material as an etch mask, thereby exposing the sensing surface.

公开(公告)号：US20180180572A1

公开(公告)日：2018-06-28

申请号：US15818718

申请日：2017-11-20

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Keith G. FIFE ,  Jordan Owens ,  Shifeng Li ,  James Bustillo

IPC: G01N27/414 ,  B01L3/00

Abstract: In one implementation, a chemical device is described. The sensor includes a chemically-sensitive field effect transistor including a floating gate structure having a plurality of floating gate conductors electrically coupled to one another. A conductive element overlies and is in communication with an uppermost floating gate conductor in the plurality of floating gate conductors. The conductive element is wider and thinner than the uppermost floating gate conductor. A dielectric material defines an opening extending to an upper surface of the conductive element.

公开(公告)号：US09989489B2

公开(公告)日：2018-06-05

申请号：US15427047

申请日：2017-02-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan Rothberg ,  Wolfgang Hinz ,  Kim Johnson ,  James Bustillo

IPC: G01N27/403 ,  G01N27/414 ,  C12Q1/68 ,  G01N33/543 ,  H01L29/423

CPC classification number: G01N27/4148 ,  C12Q1/6818 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N33/54373 ,  G01N33/5438 ,  H01L27/088 ,  H01L29/42324 ,  H01L29/78 ,  H01L2924/01006 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01073 ,  H01L2924/14 ,  H01L2924/1433 ,  Y10T436/22 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2533/101

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.