公开(公告)号：US6140050A

公开(公告)日：2000-10-31

申请号：US105907

申请日：1998-06-26

申请人： Ugur Sahin ,  Ozlem Tureci ,  Yao-Tseng Chen ,  Lloyd J. Old ,  Michael Pfreundschuh

发明人： Ugur Sahin ,  Ozlem Tureci ,  Yao-Tseng Chen ,  Lloyd J. Old ,  Michael Pfreundschuh

IPC分类号： C07K14/47 ,  C12Q1/68 ,  G01N33/574 ,  C07H21/04 ,  C12P19/34

CPC分类号： G01N33/57415 ,  C07K14/4748 ,  C12Q1/6886 ,  C12Q2600/106

摘要： The invention relates to assays for determining breast cancer or melanoma. It has been found that the accuracy of such assays can be improved by assaying samples for three or more known tumor rejection antigen precursors. For breast cancer, the tumor rejection antigen precursors known as SCP-1, NY-ESO-1, and SSX-2 are assayed. For melanoma, SSX-2, NY-ESO-1, and MAGE-3 are assayed. Additional known tumor rejection antigen precursors can also be determined to confirm the assays. It is preferred to carry these out via polymerase chain reactions.

公开(公告)号：US5977329A

公开(公告)日：1999-11-02

申请号：US24528

申请日：1998-02-17

申请人： Richard Schmidt ,  Julio C. Castro-Palomino ,  Gerd Ritter ,  Lloyd J. Old

发明人： Richard Schmidt ,  Julio C. Castro-Palomino ,  Gerd Ritter ,  Lloyd J. Old

IPC分类号： C07H15/10 ,  A61P35/00 ,  A61P37/02 ,  C07B61/00 ,  C07H1/00 ,  C07H3/06 ,  C07H13/04 ,  C07H15/18 ,  C07H15/00

CPC分类号： C07H13/04 ,  C07H1/00 ,  C07H3/06

摘要： The invention describes an improved method for making monosialoganglioside GM3 and its intermediates. Following reaction of a neuraminic acid donor and a lactose acceptor in the presence of an acid catalyst, the .alpha. and .beta. isomers of GM3 are formed. The .alpha. isomer is converted to a lactone, via action of a ring forming basic catalyst, which is then separable from the .beta. isomer. The lactone is then treated with a basic catalyst in the presence of an alcohol, to form GM3 or a GM3 intermediate.

摘要翻译： 本发明描述了制备单唾液酸神经节苷脂GM3及其中间体的改进方法。 在酸催化剂存在下，神经氨酸供体和乳糖受体反应后，形成GM3的α和β异构体。 通过形成碱性催化剂的作用，将α异构体转化成内酯，然后将其与β异构体分离。 然后在醇的存在下用碱性催化剂处理内酯，形成GM3或GM3中间体。

公开(公告)号：US5104652A

公开(公告)日：1992-04-14

申请号：US549238

申请日：1990-07-05

申请人： Alan N. Houghton ,  Karl Welte ,  Glenn Miller ,  Paul Chapman ,  Lloyd J. Old

发明人： Alan N. Houghton ,  Karl Welte ,  Glenn Miller ,  Paul Chapman ,  Lloyd J. Old

IPC分类号： A61K39/395 ,  C12N5/02

CPC分类号： A61K39/395 ,  Y10S424/809 ,  Y10S530/864

摘要： Cell surface gangliosides are presumed to play a role in cell growth and differentiation. Using monoclonal antibodies directed against G.sub.D3, a disialoganglioside expressed predominantly by cells of neuroectodermal origin, we have found that G.sub.D3 is expressed by a subpopulation of cells of the immune system including: a) fetal thymocytes in subcortical regions and near vessels, 2) lymph node lymphocytes in interfollicular areas and near vessels, and 3) a small subset of T cells in the peripheral blood. Mouse monoclonal antibodies (two IgGs, one IgM and F(ab').sub.2 fragments) reacting with G.sub.D3 were found to stimulate proliferation of T cells derived from peripheral blood. Proliferation induced by binding to G.sub.D3 could be augmented by exogenous IL-2, PMA, PHA or Protein A.

摘要翻译： 细胞表面神经节苷脂被认为在细胞生长和分化中起作用。 使用针对GD3的单克隆抗体，主要由神经外胚层起源的细胞表达的二唾液酸神经节苷脂，我们发现GD3由免疫系统细胞亚群表达，包括：a）皮质下区域和近血管的胎儿胸腺细胞，2）淋巴结 淋巴细胞在滤泡内区域和近血管，以及3）外周血中T细胞的一小部分。 发现与GD3反应的小鼠单克隆抗体（两个IgG，一个IgM和F（ab'）2片段）刺激来自外周血的T细胞的增殖。 通过与GD3结合诱导的增殖可以通过外源IL-2，PMA，PHA或蛋白A增强。

公开(公告)号：US4970299A

公开(公告)日：1990-11-13

申请号：US881630

申请日：1986-07-03

申请人： Michel Bazinet ,  Richard J. Cote ,  Lloyd J. Old

发明人： Michel Bazinet ,  Richard J. Cote ,  Lloyd J. Old

IPC分类号： A61K38/00 ,  C07K16/30 ,  G01N33/574

CPC分类号： C07K16/3038 ,  G01N33/57434 ,  A61K38/00 ,  Y10S424/804 ,  Y10S435/81 ,  Y10S530/809 ,  Y10S530/861

摘要： Four monoclonal antibodies are found which selectively identify prostate cancer. These monoclonals are therefore useful in diagnosis, differential diagnosis and treatment of prostate cancer.

摘要翻译： 发现四种单克隆抗体，其选择性地鉴定前列腺癌。 因此，这些单克隆抗体可用于诊断，鉴别诊断和治疗前列腺癌。

公开(公告)号：US4935344A

公开(公告)日：1990-06-19

申请号：US837531

申请日：1986-03-07

申请人： Neil H. Bander ,  Carlos Cordon-Cardo ,  Connie L. Finstad ,  Willet F. Whitmore ,  Myron R. Melamed ,  Herbert F. Oettgen ,  Lloyd J. Old

发明人： Neil H. Bander ,  Carlos Cordon-Cardo ,  Connie L. Finstad ,  Willet F. Whitmore ,  Myron R. Melamed ,  Herbert F. Oettgen ,  Lloyd J. Old

IPC分类号： G01N33/574

CPC分类号： G01N33/57438 ,  Y10S436/817 ,  Y10S530/809 ,  Y10S530/864

摘要： Antigenic profiles of renal carcinoma specimans developed with panels of monoclonal antibodies derived from several different tissues serve as useful clinical indicators for cancer type, cancer subset as well as histiogenesis and prognosis indicators.

摘要翻译： 由几种不同组织衍生的单克隆抗体片段开发的肾癌标本的抗原谱，可作为癌症类型，癌症亚型以及组织发生和预后指标的有用临床指标。

公开(公告)号：US4870163A

公开(公告)日：1989-09-26

申请号：US770808

申请日：1985-08-29

申请人： Berish Y. Rubin ,  Sylvia L. Anderson ,  Susan A. Sullivan ,  Lloyd J. Old ,  Barbara D. Williamson ,  Elizabeth C. Richards

发明人： Berish Y. Rubin ,  Sylvia L. Anderson ,  Susan A. Sullivan ,  Lloyd J. Old ,  Barbara D. Williamson ,  Elizabeth C. Richards

IPC分类号： G01N33/53 ,  A61K38/00 ,  A61K38/16 ,  C07K1/16 ,  C07K1/22 ,  C07K14/00 ,  C07K14/005 ,  C07K14/195 ,  C07K14/52 ,  C07K14/525 ,  C07K16/00 ,  C07K16/24 ,  C12N15/02 ,  C12P21/08 ,  C12R1/91 ,  G01N33/577

CPC分类号： C07K14/525 ,  C07K16/241 ,  Y10S530/829

摘要： Human TNF(LuKII) having a specific activity of at least 1.5.times.10.sup.5 units per milligram of total protein is produced by contacting a TNF-containing protein composition, which has been harvested from human cell lines of hematopoietic origin or recombinant origin, in separate adsorption stages with glass beads, lentil lectin bound to Sepharose, and procion red agarose, thereby selectively to adsorb TNF in each stage, while leaving some impurities unadsorbed, each contact stage being followed by contact of the adsorbent with an eluant thereby to obtain a solution of more highly purified TNF after each stage. The purified human TNF(LuKII) is used to produce monoclonal antibodies against TNF(LuKII) and such antibodies can be used to assay samples for the presence of TNF(LuKII).

摘要翻译： 通过将已经从造血起源或重组起源的人细胞系收获的含TNF的蛋白质组合物在分开的吸附阶段中接触，产生具有至少1.5×10 5单位/毫克总蛋白的比活性的人类TNF（LuKII） 用玻璃珠，扁豆凝集素结合琼脂糖和红细胞琼脂糖，从而选择性地吸附每个阶段的TNF，同时留下一些未被吸附的杂质，每个接触阶段之后接触吸附剂与洗脱剂，从而获得更多的溶液 每个阶段后高度纯化的TNF。 使用纯化的人TNF（LuKII）产生针对TNF（LuKII）的单克隆抗体，并且这种抗体可用于测定样品中TNF（LuKII）的存在。

公开(公告)号：US4865998A

公开(公告)日：1989-09-12

申请号：US83723

申请日：1987-08-10

申请人： Hans-Joachim Feickert ,  Wolfgang Rettig ,  Karen Chorney ,  Carlos Cordon-Cardo ,  Myron R. Melamed ,  Kenneth O. Lloyd ,  Herbert F. Oettgen ,  Lloyd J. Old ,  Bernd Anger

发明人： Hans-Joachim Feickert ,  Wolfgang Rettig ,  Karen Chorney ,  Carlos Cordon-Cardo ,  Myron R. Melamed ,  Kenneth O. Lloyd ,  Herbert F. Oettgen ,  Lloyd J. Old ,  Bernd Anger

IPC分类号： C07K16/30 ,  G01N33/574

CPC分类号： C07K16/3023 ,  G01N33/57423 ,  Y10S435/948

摘要： A panel of monoclonal antibodies produced from normal human lung fibroblasts and human lung tumors as immunogen is used to diagnose the presence of lung tumors and differentiate between those which are benign and those which are cancerous.

摘要翻译： 使用由正常人肺成纤维细胞和人肺肿瘤作为免疫原产生的一组单克隆抗体来诊断肺肿瘤的存在并区分良性和癌性的那些。

公开(公告)号：US4695538A

公开(公告)日：1987-09-22

申请号：US616397

申请日：1984-06-01

申请人： Richard J. Cote ,  Timothy M. Thomson ,  Alan N. Houghton ,  Herbert F. Oettgen ,  Lloyd J. Old ,  Carlos C. Cardo

发明人： Richard J. Cote ,  Timothy M. Thomson ,  Alan N. Houghton ,  Herbert F. Oettgen ,  Lloyd J. Old ,  Carlos C. Cardo

IPC分类号： G01N33/574 ,  A61K39/395 ,  C07K14/005 ,  C07K14/195 ,  C07K16/00 ,  C07K16/30 ,  C07K19/00 ,  C12N5/10 ,  C12N15/02 ,  C12P21/08 ,  C12R1/19 ,  G01N33/567 ,  G01N33/577 ,  G01N33/53

CPC分类号： G01N33/567 ,  C07K16/30 ,  Y10S435/96 ,  Y10S436/813 ,  Y10S436/828

摘要： Human monoclonal antibodies (HmAbs) capable of reacting with cell surface antigens and intracellular components are disclosed. It has been found that HmAbs Ev248, Ch-5, Ch-13, Te-39, Hu44, Ge-1, Gr-431, Gr169 and Sp909 may be used to detect these antigens in various cells. By means of these HmAbs malignant cells may be determined. This information may be used to screen metastasized tumors and primary tumors for tissue source and greatly affects the management of these cancers.

摘要翻译： 公开了能够与细胞表面抗原和胞内成分反应的人单克隆抗体（HmAb）。 已经发现HmAbs Ev248，Ch-5，Ch-13，Te-39，Hu44，Ge-1，Gr-431，Gr169和Sp909可用于检测各种细胞中的这些抗原。 通过这些HmAb可以确定恶性细胞。 该信息可用于筛选转移肿瘤和原发性肿瘤用于组织来源，并且极大地影响这些癌症的治疗。

公开(公告)号：US4650756A

公开(公告)日：1987-03-17

申请号：US297814

申请日：1981-08-31

申请人： Lloyd J. Old ,  Kenneth O. Lloyd ,  Herbert F. Oettgen ,  Willet F. Whitmore ,  Jerzy Szkudlarek ,  Connie L. Finstad ,  Donna Morrissey ,  Shun-ichiro Ogata ,  Ryuzo Ueda

发明人： Lloyd J. Old ,  Kenneth O. Lloyd ,  Herbert F. Oettgen ,  Willet F. Whitmore ,  Jerzy Szkudlarek ,  Connie L. Finstad ,  Donna Morrissey ,  Shun-ichiro Ogata ,  Ryuzo Ueda

IPC分类号： C12N15/02 ,  A61K39/395 ,  C07K14/005 ,  C07K14/195 ,  C07K14/705 ,  C07K16/00 ,  C07K16/30 ,  C07K19/00 ,  C12N5/10 ,  C12P21/08 ,  C12P21/00 ,  C12N5/00 ,  C12N15/00 ,  C12R1/91

CPC分类号： C07K16/3038 ,  Y10S435/948 ,  Y10S530/808 ,  Y10S530/809 ,  Y10S530/864

摘要： The preparation and use of monoclonal antibodies to human renal tumor cells is described. The monoclonal antibodies bind to glycoproteins of 160Kd, 120Kd and 115Kd, a glycolipid, a HLA heavy chain, group A blood and group B blood antigens.

摘要翻译： 描述了对人肾肿瘤细胞的单克隆抗体的制备和使用。 单克隆抗体结合160Kd，120Kd和115Kd的糖蛋白，糖脂，HLA重链，A组血和B组血抗原。

公开(公告)号：US4507391A

公开(公告)日：1985-03-26

申请号：US365065

申请日：1982-04-02

申请人： Clifford S. Pukel ,  Kenneth O. Lloyd ,  Luiz R. Travassos ,  Wolfgang G. Dippold ,  Herbert F. Oettgen ,  Lloyd J. Old

发明人： Clifford S. Pukel ,  Kenneth O. Lloyd ,  Luiz R. Travassos ,  Wolfgang G. Dippold ,  Herbert F. Oettgen ,  Lloyd J. Old

IPC分类号： C12Q1/04 ,  G01N33/53 ,  G01N33/574 ,  G01N33/54 ,  C12N15/00

CPC分类号： G01N33/5743 ,  G01N33/5308 ,  Y10S436/804 ,  Y10S436/811 ,  Y10S436/813 ,  Y10S436/815 ,  Y10S436/828

摘要： Mouse monoclonal antibody AbR.sub.24 (Dippold et al., Proc. Natl. Acad. Sci. 77:6114-6118, 1980) has a high degree of specificity for human melanoma cells when tested on viable cultured cells using the PA-MHA serological assay. The antigen detected by this antibody has been isolated from melanoma cells and shown to be G.sub.D3 ganglioside by compositional and partial structural analysis and by comparison with authentic G.sub.D3 by thin layer chromatography (TLC). AbR.sub.24 reacts with authentic G.sub.D3, but not with any other ganglioside tested. Using TLC and reactivity with AbR.sub.24, a wide range of cells and tissues was examined for the presence of G.sub.D3. A new serological assay, termed glycolipid-mediated immune adherence (GMIA), was devised for assaying the reactivity of AbR.sub.24 with gangliosides. Melanomas (cultured cells or tumor tissue) were shown to have T.sub.D3 and G.sub.M3 as major gangliosides. Other cells and tissues examined also contained G.sub.D3, but usually only in low amounts. Melanomas (and MOLT-4, a T-cell line) were characterized by a simplified ganglioside profile with G.sub.D3 and G.sub.M3 as major components. The apparent discrepancy between the ubiquitous presence of G.sub.D3 and the serological specificity of AbR.sub.24 for melanoma cells can be explained in terms of localization and concentration of G.sub.D3 in different cells.

摘要翻译： 当使用PA-MHA血清学测定法在活细胞培养细胞上测试时，小鼠单克隆抗体AbR24（Dippold等人，Proc.Natl.Acad.Sci.77：6114-6118,1980）对人黑素瘤细胞具有高度的特异性。 由该抗体检测到的抗原已从黑素瘤细胞中分离出来，通过组成和部分结构分析显示为GD3神经节苷脂，并通过薄层色谱（TLC）与真实的GD3进行比较。 AbR24与正常的GD3反应，但不与任何其他神经节苷脂检测。 使用TLC和与AbR24的反应性，检查广泛的细胞和组织的GD3的存在。 设计了一种称为糖脂介导的免疫粘附（GMIA）的血清学测定法，用于测定AbR24与神经节苷脂的反应性。 黑色素瘤（培养细胞或肿瘤组织）显示具有TD3和GM3作为主要神经节苷脂。 检查的其他细胞和组织也含有GD3，但通常只有少量。 黑色素瘤（和MOLT-4，T细胞系）以GD3和GM3为主要成分的简化神经节苷脂特征表征。 GD3的普遍存在与黑素瘤细胞的AbR24的血清学特异性之间的明显差异可以根据不同细胞中GD3的定位和浓度来解释。