Process for hydrolyzing starch without pH adjustment
    52.
    发明申请
    Process for hydrolyzing starch without pH adjustment 审中-公开
    水解淀粉而不进行pH调节的方法

    公开(公告)号:US20080145900A1

    公开(公告)日:2008-06-19

    申请号:US12070679

    申请日:2008-02-20

    IPC分类号: C12P19/14

    CPC分类号: C08B30/12 C12P19/14

    摘要: An alpha-amylase enzyme obtained from Bacillus acidocaldarius species is utilized to liquefy starch at a pH as low as 3.0 without the need to add thermostabilizing agents such as calcium. The alpha-amylase produces acceptable DE yields in a single liquefaction step and does not need to be inactivated prior to conducting saccharification which can proceed without adjustment of the pH of the liquefact. Alternatively, a secondary liquefaction process can be utilized, in which case two additions of the alpha-amylase are used resulting in a combined low dosage of the enzyme.

    摘要翻译: 使用从嗜酸芽孢杆菌属物种获得的α-淀粉酶在低至3.0的pH下液化淀粉,而不需要加入热稳定剂如钙。 α-淀粉酶在单一液化步骤中产生可接受的DE产率,并且在进行糖化之前不需要灭活,其可以在不调整液化的pH的情况下进行。 或者,可以使用二次液化方法,在这种情况下,使用两次加入的α-淀粉酶,导致酶的低剂量组合。

    Recovery of proteinaceous material having reduced nucleic acid levels
    54.
    发明授权
    Recovery of proteinaceous material having reduced nucleic acid levels 失效
    回收具有降低的核酸水平的蛋白质物质

    公开(公告)号:US4348479A

    公开(公告)日:1982-09-07

    申请号:US179382

    申请日:1980-08-18

    IPC分类号: A23J1/00 A23J1/18

    摘要: The present invention relates to a process which comprises recovering proteinaceous materials from nucleoprotein complexes by derivatizing the proteinaceous material with a cyclic anhydride, thereby disassocating the complex, separating the derivatized proteinaceous material from the nucleic acids and subsequently regenerating the proteinaceous material. The recovery of the proteinaceous material is accomplished by maintaining the separated derivatized proteinaceous material at a suitable acidic pH, which is a function of the reaction time and the temperature, until the derivatized proteinaceous material is disassociated into the proteinaceous material and the cyclic anhydride or most usually the equivalent acid and the proteinaceous material is recovered.

    摘要翻译: 本发明涉及一种方法,其包括通过用环酸酐衍生蛋白质物质从核蛋白复合物中回收蛋白质物质,从而分离复合物,将衍生的蛋白质物质与核酸分离,随后再生蛋白质物质。 蛋白质材料的回收是通过将分离的衍生化蛋白质材料保持在合适的酸性pH(其是反应时间和温度的函数)直到衍生的蛋白质材料分解成蛋白质材料和环状酸酐或最多 通常回收等效的酸和蛋白质物质。

    Alpha-amylase blends and methods for using said blends
    56.
    发明授权
    Alpha-amylase blends and methods for using said blends 有权
    α-淀粉酶共混物和使用所述共混物的方法

    公开(公告)号:US08507243B2

    公开(公告)日:2013-08-13

    申请号:US13119953

    申请日:2009-09-11

    IPC分类号: C12N9/26

    摘要: The present invention relates to an alpha-amylase blend, including a B. stearothermophilus alpha-amylase (AmyS) wherein the amino acid at position S242 is substituted and a B. licheniformis alpha-amylase The invention also relates to processes using the alpha-amylase blends for starch liquefaction and saccharification, ethanol production, and a sweetener production.

    摘要翻译: 本发明涉及α-淀粉酶共混物,其包括其中取代位置S242的氨基酸和地衣芽孢杆菌α-淀粉酶的嗜热脂肪芽孢杆菌α-淀粉酶(AmyS)本发明还涉及使用α-淀粉酶 用于淀粉液化和糖化,乙醇生产和甜味剂生产的共混物。

    Grain Compositions Containing Pre-Biotic Isomal To-Oligosaccharides And Methods Of Making And Using Same
    58.
    发明申请
    Grain Compositions Containing Pre-Biotic Isomal To-Oligosaccharides And Methods Of Making And Using Same 有权
    含有预生物异构异构寡糖的谷物组合物及其制备和使用方法

    公开(公告)号:US20120135108A1

    公开(公告)日:2012-05-31

    申请号:US13172744

    申请日:2011-06-29

    IPC分类号: A23L1/186 A23L1/09

    摘要: Methods for the production of substrate, tuber, and grain compositions containing isomalto-oligosaccharides are described. The methods comprise (a) contacting a substrate, tuber or grain containing ungelatinized starch with a maltogenic enzyme and a starch liquefying enzyme to produce maltose; (b) contacting said maltose with a transglucosidic enzyme, wherein said steps (a) and step (b) occur at a temperature less than or at a starch gelatinization temperature; and (c) obtaining a substrate, grain or tuber composition having an enzymatically produced isomalto-oligosaccharide, wherein the oligosaccharide is derived from the grain. The maltogenic enzyme can be either exogenous or endogenous to the grain. The contacting steps can be sequential or concurrent. The present invention also describes flour, oral rehydrating solutions, beer adjuncts, food, feed, beverage additives incorporating the grain compositions made as described.

    摘要翻译: 描述了生产含有异麦芽低聚糖的底物,块茎和谷物组合物的方法。 所述方法包括(a)使含有未凝胶化淀粉的底物,块茎或谷粒与麦芽糖酶和淀粉液化酶接触以产生麦芽糖; (b)使所述麦芽糖与转葡糖苷酶接触,其中所述步骤(a)和步骤(b)在低于淀粉糊化温度或淀粉糊化温度的温度下进行; 和(c)获得具有酶促产生的异麦芽低聚糖的底物,谷物或块茎组合物,其中寡糖衍生自谷物。 麦芽糖酶可以是外源的或内源的谷物。 接触步骤可以是顺序的或并发的。 本发明还描述了包含如上所述制备的谷物组合物的面粉,口服再水合溶液,啤酒助剂,食品,饲料,饮料添加剂。

    Process for Conversion of Granular Starch to Ethanol
    59.
    发明申请
    Process for Conversion of Granular Starch to Ethanol 审中-公开
    颗粒淀粉转化为乙醇的方法

    公开(公告)号:US20110223639A1

    公开(公告)日:2011-09-15

    申请号:US13113516

    申请日:2011-05-23

    IPC分类号: C12P19/14 C12P7/02 C12P7/06

    CPC分类号: C12P7/06 C12P19/14 Y02E50/17

    摘要: The present invention concerns a method of producing glucose from a granular starch substrate comprising, contacting a slurry comprising granular starch obtained from plant material with an alpha-amylase at a temperature below the starch gelatinization temperature of the granular starch to produce oligosaccharides and hydrolyzing the oligosaccharides to produce a mash comprising at least 20% glucose and further comprising fermenting the mash to obtain ethanol.

    摘要翻译: 本发明涉及一种从颗粒状淀粉基质制备葡萄糖的方法,包括:在低于淀粉糊化温度的淀粉糊化温度的温度下将包含从植物材料获得的颗粒状淀粉与α-淀粉酶接触的浆液接触以产生寡糖并水解低聚糖 以产生包含至少20%葡萄糖的醪液,并进一步包括发酵所述醪液以获得乙醇。

    Grain compositions containing pre-biotic isomalto-oligosaccharides and methods of making and using same
    60.
    发明授权
    Grain compositions containing pre-biotic isomalto-oligosaccharides and methods of making and using same 有权
    含有预生物异麦芽低聚糖的颗粒组合物及其制备和使用方法

    公开(公告)号:US07993689B2

    公开(公告)日:2011-08-09

    申请号:US12616595

    申请日:2009-11-11

    IPC分类号: A21D2/00 C12P19/14

    摘要: Methods for the production of substrate, tuber, and grain compositions containing isomalto-oligosaccharides are described. The methods comprise (a) contacting a substrate, tuber or grain containing ungelatinized starch with a maltogenic enzyme and a starch liquefying enzyme to produce maltose; (b) contacting the maltose with a transglucosidic enzyme, wherein the steps (a) and (b) occur at a temperature less than or at a starch gelatinization temperature; and (c) obtaining a substrate, grain or tuber composition having an enzymatically produced isomalto-oligosaccharide, wherein the oligosaccharide is derived from the grain. The maltogenic enzyme can be either exogenous or endogenous to the grain. The contacting steps can be sequential or concurrent. The present invention also describes flour, oral rehydrating solutions, beer adjuncts, food, feed, beverage additives incorporating the grain compositions made as described.

    摘要翻译: 描述了生产含有异麦芽低聚糖的底物,块茎和谷物组合物的方法。 所述方法包括(a)使含有未凝胶化淀粉的底物,块茎或谷粒与麦芽糖酶和淀粉液化酶接触以产生麦芽糖; (b)使麦芽糖与转葡糖苷酶接触,其中步骤(a)和(b)在低于淀粉糊化温度或淀粉糊化温度的温度下进行; 和(c)获得具有酶促产生的异麦芽低聚糖的底物,谷物或块茎组合物,其中寡糖衍生自谷物。 麦芽糖酶可以是外源的或内源的谷物。 接触步骤可以是顺序的或并发的。 本发明还描述了包含如上所述制备的谷物组合物的面粉,口服再水合溶液,啤酒助剂,食品,饲料,饮料添加剂。