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    Subtilisins modified at position 225 resulting in a shift in catalytic activity
    51.
    发明授权
    Subtilisins modified at position 225 resulting in a shift in catalytic activity 失效
    在225位修饰的枯草杆菌蛋白酶导致催化活性的变化

    公开(公告)号：US5155033A

    公开(公告)日：1992-10-13

    申请号：US294340

    申请日：1989-01-06

    申请人： David A. Estell ,  Robert M. Caldwell ,  Richard R. Bott ,  Thomas P. Graycar

    发明人： David A. Estell ,  Robert M. Caldwell ,  Richard R. Bott ,  Thomas P. Graycar

    IPC分类号： C12N9/56

    CPC分类号： C12N9/54

    摘要： There are described certain subtilisins wherein the amino acid sequence of such subtilisins has been modified at a position equivalent to +225 in Bacillus amyloliquefaciens, such that an amino acid selected from the group consisting of alanine, leucine, methionine, glutamine, valine, and serine, has been substituted for the amino acid residue naturally occurring at such position.

    摘要翻译： 描述了某些枯草杆菌蛋白酶，其中这种枯草杆菌蛋白酶的氨基酸序列已经在解淀粉芽孢杆菌中相当于+225的位置被修饰，使得选自丙氨酸，亮氨酸，甲硫氨酸，谷氨酰胺，缬氨酸和丝氨酸的氨基酸 已取代天然存在于该位置的氨基酸残基。

    Composition comprising various proteorhodopsins and/or bacteriorhodopsins and use thereof
    55.
    发明授权
    Composition comprising various proteorhodopsins and/or bacteriorhodopsins and use thereof 失效
    包含各种蛋白质视紫红质和/或细菌视紫红质的组合物及其用途

    公开(公告)号：US08372953B2

    公开(公告)日：2013-02-12

    申请号：US13181387

    申请日：2011-07-12

    申请人： Richard R. Bott ,  Rasmus B. Jensen ,  Bradley R. Kelemen ,  Donald E. Ward, II ,  Gregory M. Whited

    发明人： Richard R. Bott ,  Rasmus B. Jensen ,  Bradley R. Kelemen ,  Donald E. Ward, II ,  Gregory M. Whited

    IPC分类号： C07K17/00 ,  G03C1/00

    CPC分类号： G11C7/00 ,  C07K14/215 ,  C09D11/037 ,  C09D11/50 ,  G11B7/242 ,  Y10S430/146 ,  Y10T428/249921 ,  Y10T428/31623 ,  Y10T428/31685 ,  Y10T428/31725 ,  Y10T428/31783

    摘要： The present invention provides a solid material comprising an immobilized mixture of two or more proteorhodopsins, two or more bacteriorhodopsins, or one or more bacteriorhodopsin and one or more proteorhodopsins. The proteorhodopsins are selected from the group consisting of all-trans-retinal-containing proteorhodopsins and retinal analog-containing proteorhodopsins; all of which have absorption spectra that do not overlap. The bacteriorhodopsins are selected from the group consisting of all-trans-retinal-containing bacteriorhodopsins and retinal analog-containing bacteriorhodopsins; all of which have absorption spectra that do not overlap. The present invention also provides an optical information carrier, such as an optical data storage material and a fraud-proof optical data carrier, comprising the above-described solid material and a substrate selected from the group consisting of glass, paper, metal, fabric material, and plastic material, wherein said solid material is deposited on said substrate. The present invention further provides security ink comprising one or more hydrophilic polymers and a mixture of various photochromic materials.

    摘要翻译： 本发明提供了一种固体材料，其包含两种或多种蛋白质视紫红质，两种或更多种细菌视紫红质，或一种或多种细菌视紫红质和一种或多种蛋白质视紫质的固定化混合物。 蛋白质视紫红质选自全反式视黄醛的蛋白质视黄素和含视网膜类似物的蛋白质视紫红质; 所有这些都具有不重叠的吸收光谱。 细菌视紫红质选自全反式视网膜含细菌视紫红质和视网膜类似物的细菌视紫红质; 所有这些都具有不重叠的吸收光谱。 本发明还提供一种光学信息载体，例如光学数据存储材料和防欺诈光学数据载体，其包括上述固体材料和选自玻璃，纸，金属，织物材料的基材 和塑料材料，其中所述固体材料沉积在所述基底上。 本发明还提供了包含一种或多种亲水聚合物和各种光致变色材料的混合物的安全油墨。

    PROTEIN
    57.
    发明申请
    PROTEIN 有权
    蛋白

    公开(公告)号：US20120190072A1

    公开(公告)日：2012-07-26

    申请号：US13329531

    申请日：2011-12-19

    申请人： Andrei Miasnikov ,  Richard R. Bott ,  Jens Frisbaek Sørensen

    发明人： Andrei Miasnikov ,  Richard R. Bott ,  Jens Frisbaek Sørensen

    IPC分类号： C12N9/18 ,  C12P19/00 ,  C12P9/00 ,  A21D10/00 ,  A21D8/04 ,  A23L1/32 ,  A23L1/00 ,  A21D13/00 ,  C07H21/04 ,  C12S99/00

    CPC分类号： C12N9/20 ,  A21D8/042 ,  C12N9/18 ,  C12Y301/01003 ,  C12Y301/01004 ,  C12Y301/01026 ,  C12Y301/01032

    摘要： The present invention relates to a method for preparing a variant lipolytic enzyme comprising expressing in a host organism a nucleotide sequence which has at least 90% identity with a nucleotide sequence encoding a fungal lipolytic enzyme and comprises at least one modification at a position which corresponds in the encoded amino acid sequence to a) the introduction of at least one glycosylation site in the amino acid sequence compared with the original fungal lipolytic enzyme; b) the introduction of at least one amino acid at a surface position and at a location in an external loop distal to the active site of the enzyme which is more hydrophilic; or c) a substitution or insertion at one or more of positions disclosed herein or a deletion at one or more positions disclosed herein. The invention also relates to polypeptide produced by the method and to novel nucleic acids.

    摘要翻译： 本发明涉及一种制备变体脂肪分解酶的方法，包括在宿主生物体中表达与编码真菌脂肪分解酶的核苷酸序列具有至少90％同一性的核苷酸序列，并且包含至少一个在 编码的氨基酸序列以a）与原始真菌脂肪分解酶相比，在氨基酸序列中引入至少一个糖基化位点; b）在更亲水的酶的活性位点的远端的外部环的表面位置和位置处引入至少一个氨基酸; 或c）在本文公开的一个或多个位置的取代或插入或在本文公开的一个或多个位置处的缺失。 本发明还涉及通过该方法产生的多肽和新的核酸。