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1.发明授权Subtilisins modified at position 225 resulting in a shift in catalytic activity 失效在225位修饰的枯草杆菌蛋白酶导致催化活性的变化
公开(公告)号：US5155033A
公开(公告)日：1992-10-13
申请号：US294340
申请日：1989-01-06
IPC分类号： C12N9/56
CPC分类号： C12N9/54
摘要： There are described certain subtilisins wherein the amino acid sequence of such subtilisins has been modified at a position equivalent to +225 in Bacillus amyloliquefaciens, such that an amino acid selected from the group consisting of alanine, leucine, methionine, glutamine, valine, and serine, has been substituted for the amino acid residue naturally occurring at such position.
摘要翻译： 描述了某些枯草杆菌蛋白酶，其中这种枯草杆菌蛋白酶的氨基酸序列已经在解淀粉芽孢杆菌中相当于+225的位置被修饰，使得选自丙氨酸，亮氨酸，甲硫氨酸，谷氨酰胺，缬氨酸和丝氨酸的氨基酸 已取代天然存在于该位置的氨基酸残基。
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2.发明授权Non-human carbonyl hydrolase mutants, DNA sequences and vectors encoding same and hosts transformed with said vectors 失效非人羰基水解酶突变体，DNA序列和编码相同的载体以及用所述载体转化的宿主
公开(公告)号：US5316941A
公开(公告)日：1994-05-31
申请号：US896818
申请日：1992-04-29
CPC分类号： C12N9/54
摘要： There are described certain DNA sequences which encode subtilisins wherein the amino acid sequence of such substilisins has been modified at a position equivalent to +225 in Bacillus amyloliquefaciens, such that an amino acid selected from the group consisting of alanine, leucine, methionine, glutamine, valine and serine, has been substituted for the amino acid residues naturally occuring at such position.
摘要翻译： 描述了编码枯草杆菌蛋白酶的某些DNA序列，其中这种变形蛋白酶的氨基酸序列已在解淀粉芽孢杆菌中相当于+225的位置被修饰，使得选自丙氨酸，亮氨酸，甲硫氨酸，谷氨酰胺， 缬氨酸和丝氨酸已被替代在此位置天然存在的氨基酸残基。
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公开(公告)号：US5204015A
公开(公告)日：1993-04-20
申请号：US958885
申请日：1992-10-09
CPC分类号： C12R1/07 , C11D3/386 , C12N15/102 , C12N15/75 , C12N9/54
摘要： Novel carbonyl hydrolase mutants derived from the DNA sequences of naturally-occurring or recombinant non-human carbonyl hydrolases are disclosed. The mutant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to generate the substitution of one or more amino acid residues in the amino acid sequence of a precursor carbonyl hydrolase. Such mutant carbonyl hydrolases have properties which are different from those of the precursor hydrolase and are especially useful in detergent formulations. The substituted amino acid residues correspond to position +123 and/or +274 in Bacillus amyloliquefaciens subtilisin.
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公开(公告)号：US5185258A
公开(公告)日：1993-02-09
申请号：US600430
申请日：1990-10-19
IPC分类号： C07K14/195 , C07K14/00 , C07K14/32 , C07K14/41 , C11D20060101 , C11D3/386 , C11D10/00 , C12N1/21 , C12N9/00 , C12N9/14 , C12N9/16 , C12N9/20 , C12N9/50 , C12N9/54 , C12N9/56 , C12N15/00 , C12N15/09 , C12N15/10 , C12N15/57 , C12N15/75 , C12P7/64 , C12P21/02
CPC分类号： C11D3/38609 , C11D3/386 , C12N15/102 , C12N15/75 , C12N9/14 , C12N9/16 , C12N9/20 , C12N9/50 , C12N9/54 , C12P7/6418 , C12R1/07
摘要： Novel carbonyl hydrolase mutants derived from the DNA sequences of naturally-occurring or recombinant non-human carbonyl hydrolases are disclosed. The mutant carbonyl hydrolases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant carbonyl hydrolase to generate the substitution of one or more amino acid residues in the amino acid sequence of a precursor carbonyl hydrolase. Such mutant carbonyl hydrolases have properties which are different from those of the precursor hydrolase and are especially useful in detergent formulations. The substituted amino acid residues correspond to position +123 and/or +274 in Bacillus amyloliquefaciens subtilisin.
摘要翻译： 公开了衍生自天然存在或重组非人羰基水解酶的DNA序列的新型羰基水解酶突变体。 突变体羰基水解酶通常通过体外修饰编码天然存在或重组羰基水解酶的前体DNA序列获得，以产生前体羰基水解酶的氨基酸序列中一个或多个氨基酸残基的取代。 这种突变型羰基水解酶具有与前体水解酶不同的性质，并且在洗涤剂配方中特别有用。 取代的氨基酸残基对应于解淀粉芽孢杆菌枯草杆菌蛋白酶中的位置+123和/或+ 274。
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5.发明授权
公开(公告)号：US6033890A
公开(公告)日：2000-03-07
申请号：US445463
申请日：1995-05-22
申请人： Edward Benjamin Jakobovits , Joy L. Silen , Mark J. Levy , Thomas C. Goodman , Martin Becker , Edwin F. Ullman , Robert M. Caldwell , Richard R. Bott , Christopher Charles Barnett
发明人： Edward Benjamin Jakobovits , Joy L. Silen , Mark J. Levy , Thomas C. Goodman , Martin Becker , Edwin F. Ullman , Robert M. Caldwell , Richard R. Bott , Christopher Charles Barnett
IPC分类号： G01N33/542 , C12N9/04 , C12N15/09 , C12Q1/28 , C12Q1/32 , C12R1/19 , G01N33/535 , G01N33/569 , G01N33/58 , G01N33/78 , G01N33/94
CPC分类号： C12N9/0006 , C12Q1/28 , G01N33/535 , G01N33/56911 , G01N33/581 , G01N33/78 , G01N33/94 , G01N2333/904
摘要： The present invention relates to methods for immunoassay of analytes employing mutant glucose-6-phosphate dehydrogenase (G6PDH) enzymes as labels. In particular, the invention relates to the use of conjugates of an analyte or analyte analog and a mutant NAD.sup.+ dependent G6PDH differing from any precursor G6PDH by the deletion, substitution, or insertion, or any combination thereof of at least one amino acid per subunit. The invention also involves the construction of several mutations in precursor glucose-6-phosphate dehydrogenase (G6PDH) enzymes. Typically, the mutations involve deletion or substitution of one or more lysine residues, or introduction of one or more cysteine residues by insertion of cysteine to precursor G6PDH or substitution of precursor G6PDH amino acids residues with cysteine. The present invention also relates to conjugates of the subject enzymes and specific binding pair members, kits useful in performing the methods of the invention, cell lines producing the subject enzymes, DNA sequences encoding the subject enzymes, and vectors containing DNA encoding the subject enzymes and designed to allow a host cell to produce the subject enzymes.
摘要翻译： 本发明涉及使用突变型葡萄糖-6-磷酸脱氢酶（G6PDH）酶作为标记免疫测定分析物的方法。 特别地，本发明涉及通过每个亚基的至少一个氨基酸的缺失，取代或插入或其任何组合，使用不同于任何前体G6PDH的分析物或分析物类似物和突变体NAD +依赖性G6PDH的缀合物的用途。 本发明还涉及前体葡萄糖-6-磷酸脱氢酶（G6PDH）酶的几个突变的构建。 通常，突变涉及一个或多个赖氨酸残基的缺失或取代，或通过将半胱氨酸插入前体G6PDH或用半胱氨酸取代前体G6PDH氨基酸残基引入一个或多个半胱氨酸残基。 本发明还涉及本发明的酶和特异性结合对成员的缀合物，可用于实施本发明方法的试剂盒，产生本发明酶的细胞系，编码本发明酶的DNA序列，和含有编码本发明酶的DNA的载体和 旨在使宿主细胞产生目标酶。
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6.发明授权
公开(公告)号：US6090567A
公开(公告)日：2000-07-18
申请号：US445464
申请日：1995-05-22
申请人： Edward Benjamin Jakobovits , Joy L. Silen , Mark J. Levy , Thomas C. Goodman , Martin Becker , Edwin F. Ullman , Robert M. Caldwell , Richard R. Bott , Christopher Charles Barnett
发明人： Edward Benjamin Jakobovits , Joy L. Silen , Mark J. Levy , Thomas C. Goodman , Martin Becker , Edwin F. Ullman , Robert M. Caldwell , Richard R. Bott , Christopher Charles Barnett
IPC分类号： G01N33/542 , C12N9/04 , C12N15/09 , C12Q1/28 , C12Q1/32 , C12R1/19 , G01N33/535 , G01N33/569 , G01N33/58 , G01N33/78 , G01N33/94 , G01N33/53
CPC分类号： C12N9/0006 , C12Q1/28 , G01N33/535 , G01N33/56911 , G01N33/581 , G01N33/78 , G01N33/94 , G01N2333/904
摘要： Methods for immunoassay of analytes employing mutant glucose-6-phosphate dehydrogenase (G6PDH) enzymes as labels. In particular, the invention relates to the use of conjugates of an analyte or analyte analog and a mutant NAD.sup.+ dependent G6PDH differing from any precursor G6PDH by the deletion, substitution, or insertion, or any combination thereof of at least one amino acid per subunit. The invention also involves the construction of several mutations in precursor glucose-6-phosphate dehydrogenase (G6PDH) enzymes. Typically, the mutations involve deletion or substitution of one or more lysine residues, or introduction of one or more cysteine residues by insertion of cysteine to precursor G6PDH or substitution of precursor G6PDH amino acids residues with cysteine. The present invention also relates to conjugates of the subject enzymes and specific binding pair members, kits useful in performing the methods of the invention, cell lines producing the subject enzymes, DNA sequences encoding the subject enzymes, and vectors containing DNA encoding the subject enzymes and designed to allow a host cell to produce the subject enzymes.
摘要翻译： 使用突变型葡萄糖-6-磷酸脱氢酶（G6PDH）酶作为标记免疫测定分析物的方法。 特别地，本发明涉及通过每个亚基的至少一个氨基酸的缺失，取代或插入或其任何组合，使用不同于任何前体G6PDH的分析物或分析物类似物和突变体NAD +依赖性G6PDH的缀合物的用途。 本发明还涉及前体葡萄糖-6-磷酸脱氢酶（G6PDH）酶的几个突变的构建。 通常，突变涉及一个或多个赖氨酸残基的缺失或取代，或通过将半胱氨酸插入前体G6PDH或用半胱氨酸取代前体G6PDH氨基酸残基引入一个或多个半胱氨酸残基。 本发明还涉及本发明的酶和特异性结合对成员的缀合物，可用于实施本发明方法的试剂盒，产生本发明酶的细胞系，编码本发明酶的DNA序列，和含有编码本发明酶的DNA的载体和 旨在使宿主细胞产生目标酶。
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7.发明授权公开(公告)号：US06455288B1
公开(公告)日：2002-09-24
申请号：US08044857
申请日：1993-04-08
申请人： Edward Benjamin Jakobovits , Joy L. Silen , Mark J. Levy , Thomas C. Goodman , Martin Becker , Edwin F. Ullman , Robert M. Caldwell , Richard R. Bott , Christopher Charles Barnett
发明人： Edward Benjamin Jakobovits , Joy L. Silen , Mark J. Levy , Thomas C. Goodman , Martin Becker , Edwin F. Ullman , Robert M. Caldwell , Richard R. Bott , Christopher Charles Barnett
IPC分类号： C12N1552
CPC分类号： C12N9/0006 , C12Q1/28 , G01N33/535 , G01N33/56911 , G01N33/581 , G01N33/78 , G01N33/94 , G01N2333/904
摘要： The present invention relates to methods for immunoassay of analytes employing mutant glucose-6-phosphate dehydrogenase (G6PDH) enzymes as labels. In particular, the invention relates-to the use of conjugates of an analyte or analyte analog and a mutant NAD+ dependent G6PDH differing from any precursor G6PDH by the deletion, substitution, or insertion, or any combination thereof of at least one amino acid per subunit. The invention also involves the construction of several mutations in precursor glucose-6-phosphate dehydrogenase (G6PDH) enzymes. Typically, the mutations involve deletion or substitution of one or more lysine residues, or introduction of one or more cysteine residues by insertion of cysteine to precursor G6PDH or substitution of precursor G6PDH amino acids residues with cysteine. The present invention also relates to conjugates of the subject enzymes and specific binding pair members, kits useful in performing the methods of the invention, cell lines producing the subject enzymes, DNA sequences encoding the subject enzymes, and vectors containing DNA encoding the subject enzymes and designed to allow a host cell to produce the subject enzymes.
摘要翻译： 本发明涉及使用突变型葡萄糖-6-磷酸脱氢酶（G6PDH）酶作为标记免疫测定分析物的方法。 特别地，本发明涉及分离物或分析物类似物和与任何前体G6PDH不同的突变体NAD +依赖性G6PDH通过每个亚基的缺失，取代或插入或其任何组合的至少一个氨基酸的缀合物的用途 。 本发明还涉及前体葡萄糖-6-磷酸脱氢酶（G6PDH）酶的几个突变的构建。 通常，突变涉及一个或多个赖氨酸残基的缺失或取代，或通过将半胱氨酸插入前体G6PDH或用半胱氨酸取代前体G6PDH氨基酸残基引入一个或多个半胱氨酸残基。 本发明还涉及本发明的酶和特异性结合对成员的缀合物，可用于实施本发明方法的试剂盒，产生本发明酶的细胞系，编码本发明酶的DNA序列，和含有编码本发明酶的DNA的载体和 旨在使宿主细胞产生目标酶。
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公开(公告)号：US20120064586A1
公开(公告)日：2012-03-15
申请号：US13228758
申请日：2011-09-09
摘要： The present invention concerns multimeric oxidoreductase complexes which function in the enzymatic conversion of a carbon substrate, said complexes having a dehydrogenase subunit and a cytochrome C subunit. The invention further relates to polynucleotides coding for the multimeric complexes and methods of use thereof.
摘要翻译： 本发明涉及在碳底物的酶促转化中起作用的多聚体氧化还原酶复合物，所述复合物具有脱氢酶亚基和细胞色素C亚基。 本发明还涉及编码多聚体复合物的多核苷酸及其使用方法。
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公开(公告)号：US06211134B1
公开(公告)日：2001-04-03
申请号：US08985659
申请日：1997-12-09
IPC分类号： C11D3386
CPC分类号： C12N9/2417
摘要： Novel &agr;-amylase enzymes are disclosed having a substution equivalent to G475R in Bacillus licheniformis. The disclosed &agr;-amylase enzymes show improved specific activity and starch hydrolysis performance. Also provided are polynucleotides encoding such enzymes, expression vectors including such polynucleotides, host cells transformed with such expression vectors, and the use of such enzymes in detergent compositions.
摘要翻译： 公开了具有等同于地衣芽孢杆菌中的G475R的分子的新型α-淀粉酶。 所公开的α-淀粉酶显示改善的比活性和淀粉水解性能。 还提供了编码这些酶的多核苷酸，包括这种多核苷酸的表达载体，用这种表达载体转化的宿主细胞，以及这些酶在洗涤剂组合物中的用途。
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公开(公告)号：US08349599B2
公开(公告)日：2013-01-08
申请号：US13228758
申请日：2011-09-09
摘要： Described are multimeric oxidoreductase complexes which function in the enzymatic conversion of a carbon substrate. The complexes having a dehydrogenase subunit and a cytochrome C subunit. Also described are polynucleotides coding for the multimeric complexes and methods of use thereof.
摘要翻译： 描述了在碳底物的酶转化中起作用的多聚体氧化还原酶复合物。 具有脱氢酶亚基和细胞色素C亚基的复合物。 还描述了编码多聚体复合物的多核苷酸及其使用方法。
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