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133 条记录 (耗时 0.04 秒)

    Monomeric and dimeric fluorescent protein variants and methods for making same
    52.
    发明授权
    Monomeric and dimeric fluorescent protein variants and methods for making same 有权
    单体和二聚体荧光蛋白变体及其制备方法

    公开(公告)号：US07687614B2

    公开(公告)日：2010-03-30

    申请号：US10931304

    申请日：2004-08-30

    申请人： Robert E. Campbell ,  Nathan C. Shaner ,  Roger Y. Tsien

    发明人： Robert E. Campbell ,  Nathan C. Shaner ,  Roger Y. Tsien

    IPC分类号： C07H21/04 ,  A61K38/00

    CPC分类号： C07K14/43595 ,  C12Q1/25 ,  G01N33/585 ,  G01N33/84 ,  G01N2500/02

    摘要： The present invention relates generally to fluorescent proteins and fluorescent protein variants, and more specifically to monomeric and dimeric forms of Anthozoan fluorescent proteins. In one aspect, the present invention provides variants of fluorescent proteins, where the variants have a reduced propensity to tetramerize, and form dimeric or monomeric structures. In a further aspect, the present invention provides variants of fluorescent proteins, the variants being characterized by more efficient maturation than corresponding fluorescent proteins from which they are derived. The invention also relates to methods of making and using such fluorescent proteins and fluorescent protein variants, including fluorescent protein monomers and dimers.

    摘要翻译： 本发明一般涉及荧光蛋白和荧光蛋白变体，更具体地涉及Anthozoan荧光蛋白的单体和二聚体形式。 在一个方面，本发明提供荧光蛋白的变体，其中变体具有降低的四聚体倾向并形成二聚体或单体结构。 在另一方面，本发明提供荧光蛋白的变体，所述变体的特征在于比它们衍生的相应荧光蛋白更有效的成熟。 本发明还涉及制备和使用这些荧光蛋白和荧光蛋白变体（包括荧光蛋白单体和二聚体）的方法。

    Non-oligomerizing tandem fluorescent proteins
    54.
    发明授权
    Non-oligomerizing tandem fluorescent proteins 有权
    非低聚串联荧光蛋白

    公开(公告)号：US07332598B2

    公开(公告)日：2008-02-19

    申请号：US10885988

    申请日：2004-07-06

    申请人： Roger Y. Tsien ,  Robert E. Campbell

    发明人： Roger Y. Tsien ,  Robert E. Campbell

    IPC分类号： C07H21/04 ,  C07K14/00 ,  C12N15/09

    CPC分类号： C07K14/43504 ,  C07K14/43595 ,  C12Q1/25 ,  G01N33/585 ,  G01N33/84 ,  G01N2500/02

    摘要： Non-oligomerizing fluorescent proteins, which are formed by operatively linking two or more monomers of a fluorescent protein, or which are derived from a fluorescent protein having at least one mutation that reduces or eliminates the ability of the fluorescent protein to oligomerize, are provided. The non-oligomerizing fluorescent proteins can be derived from a naturally occurring green fluorescent protein, a red fluorescent protein, or other fluorescent protein, or a fluorescent protein related thereto. Also provided is a fusion protein, which includes a non-oligomerizing fluorescent protein linked to at least one polypeptide of interest. In addition, a polynucleotide encoding a non-oligomerizing fluorescent protein is provided, as is a recombinant nucleic acid molecule, which includes polynucleotide encoding a non-oligomerizing fluorescent protein operatively linked to at least a second polynucleotide. Vectors and host cells containing such polynucleotides also are provided, as are kits containing one or more non-oligomerizing fluorescent proteins or encoding polynucleotides or constructs derived therefrom. Further provided are methods of making and using the proteins and polynucleotides.

    摘要翻译： 提供了通过可操作地连接两种或更多种荧光蛋白单体或衍生自具有降低或消除荧光蛋白低聚的能力的至少一种突变的荧光蛋白而形成的非低聚荧光蛋白。 非低聚荧光蛋白可以衍生自天然存在的绿色荧光蛋白，红色荧光蛋白或其他荧光蛋白，或与其相关的荧光蛋白。 还提供了融合蛋白，其包括与至少一种目的多肽连接的非低聚荧光蛋白。 此外，提供编码非低聚荧光蛋白的多核苷酸，重组核酸分子也包括编码与至少第二多核苷酸有效连接的非低聚荧光蛋白的多核苷酸。 还提供了含有这种多核苷酸的载体和宿主细胞，以及含有一种或多种非低聚荧光蛋白或编码多核苷酸或由其衍生的构建体的试剂盒。 进一步提供制备和使用蛋白质和多核苷酸的方法。

    Synthetic molecules for labeling histidine-rich proteins
    56.
    发明授权
    Synthetic molecules for labeling histidine-rich proteins 失效
    用于标记富含组氨酸的蛋白质的合成分子

    公开(公告)号：US07041821B2

    公开(公告)日：2006-05-09

    申请号：US10759762

    申请日：2004-01-16

    申请人： Roger Y. Tsien ,  Christina Hauser

    发明人： Roger Y. Tsien ,  Christina Hauser

    IPC分类号： C07D265/38 ,  C07D311/80

    CPC分类号： C07D405/12

    摘要： The invention provides Zn-chelating compounds that are molecularly engineered to bind to a specific target sequence in a protein of interest. The Zn2+ ion is far less toxic and promiscuous than nickel and therefore provides an attractive alternative to Ni-based labeling systems. Invention Zn-chelating compounds also do not require oxidizable thiols and therefore can be used in non-reducing environments such as the surface of living cells. In addition, the target sequence is genetically encodable and requires incorporation of only a few amino acids, unlike fusions to fluorescent proteins such as GFP.

    摘要翻译： 本发明提供分子工程化以结合目标蛋白质中特定靶序列的Zn螯合化合物。 Zn 2+离子的毒性远低于镍，因此提供了Ni基标记体系的有吸引力的替代方案。 本发明的锌螯合化合物也不需要可氧化硫醇，因此可用于非还原性环境如活细胞表面。 此外，靶序列是遗传编码的，并且需要仅掺入少数氨基酸，不同于与荧光蛋白如GFP的融合。

    Synthetic molecules for labeling histidine-rich proteins
    58.
    发明授权
    Synthetic molecules for labeling histidine-rich proteins 失效
    用于标记富含组氨酸的蛋白质的合成分子

    公开(公告)号：US06933384B2

    公开(公告)日：2005-08-23

    申请号：US10346658

    申请日：2003-01-16

    申请人： Roger Y. Tsien ,  Christina Hauser

    发明人： Roger Y. Tsien ,  Christina Hauser

    IPC分类号： C07D405/12 ,  C07D265/38 ,  C07D311/80 ,  C07D405/14

    CPC分类号： C07D405/12

    摘要： The invention provides Zn-chelating compounds that are molecularly engineered to bind to a specific target sequence in a protein of interest. The Zn2+ ion is far less toxic and promiscuous than nickel and therefore provides an attractive alternative to Ni-based labeling systems. Invention Zn-chelating compounds also do not require oxidizable thiols and therefore can be used in non-reducing environments such as the surface of living cells. In addition, the target sequence is genetically encodable and requires incorporation of only a few amino acids, unlike fusions to fluorescent proteins such as GFP.

    摘要翻译： 本发明提供分子工程化以结合目标蛋白质中特定靶序列的Zn螯合化合物。 Zn 2+离子的毒性远低于镍，因此提供了Ni基标记体系的有吸引力的替代方案。 本发明的锌螯合化合物也不需要可氧化硫醇，因此可用于非还原性环境如活细胞表面。 此外，靶序列是遗传编码的，并且需要仅掺入少数氨基酸，不同于与荧光蛋白如GFP的融合。

    Tandem fluorescent protein constructs
    59.
    发明授权
    Tandem fluorescent protein constructs 失效
    串联荧光蛋白构建体

    公开(公告)号：US06803188B1

    公开(公告)日：2004-10-12

    申请号：US08594575

    申请日：1996-01-31

    申请人： Roger Y. Tsien ,  Roger Heim

    发明人： Roger Y. Tsien ,  Roger Heim

    IPC分类号： C07K1400

    CPC分类号： C12Q1/485 ,  C07K14/43595 ,  C07K2319/00 ,  C12Q1/37 ,  G01N33/542 ,  G01N2333/43595

    摘要： This invention provides tandem fluorescent protein construct including a donor fluorescent protein moiety, an acceptor fluorescent protein moiety and a linker moiety that couples the donor and acceptor moieties. The donor and acceptor moieties exhibit fluorescence resonance energy transfer which is eliminated upon cleavage. The constructs are useful in enzymatic assays.

    摘要翻译： 本发明提供了包括供体荧光蛋白部分，受体荧光蛋白部分和连接供体和受体部分的接头部分的串联荧光蛋白构建体。 供体和受体部分表现出在切割时消除的荧光共振能量转移。 所述构建体可用于酶测定。