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公开(公告)号:US20120052488A1
公开(公告)日:2012-03-01
申请号:US13208151
申请日:2011-08-11
Applicant: Stephen Yue , Robert Weber , Xiangxu Kong , Andrei Fedorov , John Lyle
Inventor: Stephen Yue , Robert Weber , Xiangxu Kong , Andrei Fedorov , John Lyle
IPC: C12Q1/68 , C07D405/14 , C07C243/14 , C07C63/04 , C07C309/19
CPC classification number: C12Q1/6869 , C12N13/00 , C12Q1/25 , G01N33/52 , G01N33/54373 , G01N2333/91245 , G01N2333/916
Abstract: Compositions, devices, systems and methods for reducing and/or preventing photo-induced damage of one or more reactants in an illuminated analytical reaction by addition of one or more photoprotective compounds to the reaction mixture and allowing the reaction to proceed for a period that is less than a photo-induced damage threshold period.
Abstract translation: 用于通过向反应混合物中加入一种或多种光保护性化合物并允许反应进行一段时间,以减少和/或防止照明分析反应中的一种或多种反应物的光诱导损伤的组合物,装置,系统和方法 小于光致伤害阈值期。
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公开(公告)号:US07842811B2
公开(公告)日:2010-11-30
申请号:US12686149
申请日:2010-01-12
Applicant: Jason Dallwig , David Hagen , Shih-Jung Huang , Gerald Thomas , Stephen Yue
Inventor: Jason Dallwig , David Hagen , Shih-Jung Huang , Gerald Thomas , Stephen Yue
IPC: C07D215/38 , C12Q1/68 , C07H21/02 , C07H21/04
CPC classification number: C09B23/02 , C12Q1/6827 , G01N33/582 , Y10T436/143333 , C12Q2527/125 , C12Q2563/107
Abstract: Cyanine dye compounds having a negatively charged substituent that are nucleic acid stains, particularly for fluorescent staining of DNA, including compounds having the formula wherein W forms one or two fused 5- or 6-membered aromatic rings, α has a value of 0 or 1, n has a value of 0, or 1, X is O, S, or Se, and D is a pyridinium, or quinolinium moiety, provided that the compound has at least one negatively charged substituent.
Abstract translation: 具有带负电荷的取代基的花青染料化合物,其特征在于DNA的荧光染色,包括具有下式的化合物,其中W形成一个或两个稠合的5-或6-元芳环,α为0或1 ,n为0或1,X为O,S或Se,D为吡啶鎓或喹啉鎓部分,条件是该化合物具有至少一个带负电荷的取代基。
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公开(公告)号:US20090047683A1
公开(公告)日:2009-02-19
申请号:US12240218
申请日:2008-09-29
Applicant: Jason Alfred Dallwig , David Carl Hagen , Shih-Jung Huang , Gerald Alan Thomas , Stephen Yue
Inventor: Jason Alfred Dallwig , David Carl Hagen , Shih-Jung Huang , Gerald Alan Thomas , Stephen Yue
CPC classification number: C09B23/02 , C12Q1/6827 , G01N33/582 , Y10T436/143333 , C12Q2527/125 , C12Q2563/107
Abstract: Cyanine dye compounds having a negatively charged substituent that are nucleic acid stains, particularly for fluorescent staining of DNA, including compounds having the formula wherein W forms one or two fused 5- or 6-membered aromatic rings, α has a value of 0 or 1, n has a value of 0, or 1, X is O, S, or Se, and D is a pyridinium, or quinolinium moiety, provided that the compound has at least one negatively charged substituent.
Abstract translation: 具有带负电荷的取代基的花青染料化合物,特别是用于DNA的荧光染色的带负电荷的取代基,包括具有下式的化合物,其中W形成一个或两个稠合的5-或6-元芳环,α具有0或1的值 ,n为0或1,X为O,S或Se,D为吡啶鎓或喹啉鎓部分,条件是该化合物具有至少一个带负电荷的取代基。
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公开(公告)号:US20050214560A1
公开(公告)日:2005-09-29
申请号:US10808753
申请日:2004-03-25
Applicant: Stephen Yue , Mark Kleckner , Maurice Bechard , Joseph Fader , Luiz Lopes , William Elders , Timothy Sowinski , Leo Medeiros , Pete Kiousis
Inventor: Stephen Yue , Mark Kleckner , Maurice Bechard , Joseph Fader , Luiz Lopes , William Elders , Timothy Sowinski , Leo Medeiros , Pete Kiousis
CPC classification number: B60G21/0551 , B60G11/20 , B60G17/025 , B60G2204/44 , B60G2204/45 , B60G2206/427 , B60G2206/8103 , B60G2206/81035 , B60G2206/8112 , B60G2206/8402 , B60G2206/8403 , B60G2500/20 , F16F1/16 , Y10T428/12389 , Y10T428/12486
Abstract: A manufacturing process produces a stabilizer bar having a thickened, reinforced portion formed by thermal spraying a molten metal onto a surface of the bar. The process produces a stabilizer bar having a thickened portion in the areas typically subject to fatigue failure while avoiding the addition of unnecessary material and weight to other locations of the stabilizer bar not needing reinforcement. In one example, the unprocessed bar stock is thermally sprayed in desired locations. The thermally sprayed bar is formed into a desired shape and heat treated. The heat treated, formed, and sprayed bar is shot peened. Alternatively, the bar is thermally sprayed after the surface has been prepared by shot peening. Forming of the bar may also occur before thermal spraying, and pretreated bar stock may also be used.
Abstract translation: 制造过程产生具有通过将熔融金属热喷涂到棒的表面上形成的增厚的增强部分的稳定杆。 该方法产生在通常遭受疲劳破坏的区域中具有加厚部分的稳定杆,同时避免不必要的材料和重量添加到不需要加强的稳定杆的其它位置。 在一个实例中,将未处理的棒材在期望的位置热喷涂。 将热喷涂棒形成为所需形状并进行热处理。 热处理,成型和喷涂的棒材是喷丸硬化的。 或者,在通过喷丸硬化制备表面后,将棒进行热喷涂。 棒的形成也可以在热喷涂之前进行,并且也可以使用预处理的棒料。
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公开(公告)号:US5200005A
公开(公告)日:1993-04-06
申请号:US652872
申请日:1991-02-08
Applicant: Abbas Najah-Zadeh , John J. Jonas , Stephen Yue
Inventor: Abbas Najah-Zadeh , John J. Jonas , Stephen Yue
CPC classification number: C21D8/0231 , C21D8/00 , B21B2201/04 , C21D2211/005 , C21D8/0226
Abstract: The strength of interstitial free steels is increased by up to 100% and the ductile to brittle transition temperature is decreased by up to 100.degree. C. by warm finish rolling in the single phase ferrite region below A.sub.rl to effect ferrite dynamic recrystallization of the steel microstructure to a ferrite structure of grain size having a grain size of up to 5 .mu.m, and especially an ultra fine grain size of 1 to 2 .mu.m; the method may be employed in various hot working methods including strip and rod mills, planetary hot rolling and extrusion.
Abstract translation: 无间隙钢的强度提高高达100%,延性至脆性转变温度通过在Arl以下的单相铁素体区域中的热精轧降低高达100℃,以实现钢微观结构的铁素体动态再结晶 涉及粒径高达5μm的粒径的铁素体结构,特别是1〜2μm的超细晶粒度; 该方法可以用于各种热加工方法,包括带钢和棒磨机,行星式热轧和挤压。
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公开(公告)号:US4840051A
公开(公告)日:1989-06-20
申请号:US55945
申请日:1987-06-01
Applicant: Francisco Boratto , Stephen Yue , John J. Jonas
Inventor: Francisco Boratto , Stephen Yue , John J. Jonas
CPC classification number: B21B1/32 , B21B1/26 , B21B2201/04 , B21B2265/22 , Y10T70/375
Abstract: A series of thermomechanical workings such as temperature-controlled torsional strains are applied to a specimen of steel at strain and temperature levels and interpass times selected to simulate rolling mill conditions. The measured stress values are compared with the temperatures of the steel during the working periods during which the respective values were obtained. Thermomechanical working schedules are repeated at selected varying starting and terminating temperatures thereby to obtain a series of possible rolling schedules. These simulations are selected so that a varying number of reduction passes in the sequence occur at steel temperatures below temperature A.sub.r3. The value of a selected parameter of the worked steel, e.g. yield strength, is measured at ambient temperature. From the rolling mill analogue of possible rolling schedule simulations, an optimized rolling schedule is selected which will predictably impart to the steel a value of the selected parameter falling within a predetermined range. Linear regression analysis is applied to empirically obtained rolling mill data to derive one or more linear relationships between a selected property (e.g. yield strength) of the steel and rolling mill parameters thereby to permit selection of an optimum rolling schedule suitable to obtain a preselected value of the selected property of the steel.
Abstract translation: 将温度控制的扭转应变的一系列热机械工作应用于钢的样品的应变和温度水平以及所选择的插层时间以模拟轧机条件。 将测量的应力值与获得各个值的工作期间的钢的温度进行比较。 在选择的变化的起始和终止温度下重复热机械工作时间表,从而获得一系列可能的轧制时间表。 选择这些模拟,使得在低于温度Ar 3的钢温度下,顺序发生不同数量的还原。 加工钢的选定参数的值,例如 屈服强度,在环境温度下测量。 从可能的轧制计划模拟的轧机模拟中,选择优化的轧制计划,其可预测地赋予钢所选参数的值落在预定范围内。 线性回归分析被应用于经验获得的轧机数据以导出钢的选定性质(例如屈服强度)与轧机参数之间的一个或多个线性关系,从而允许选择适于获得预定值 钢的选定属性。
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公开(公告)号:US08906612B2
公开(公告)日:2014-12-09
申请号:US13218382
申请日:2011-08-25
Applicant: Gene Shen , Lubomir Sebo , Stephen Yue , Wei-Chuan Sun , Louis Brogley , Andrei Fedorov
Inventor: Gene Shen , Lubomir Sebo , Stephen Yue , Wei-Chuan Sun , Louis Brogley , Andrei Fedorov
IPC: C12Q1/68 , C07H21/00 , C07H21/02 , C07H21/04 , C09B23/06 , C07D209/14 , C09B23/01 , C09B23/08 , G01N33/58 , C07D401/14 , C07D405/14
CPC classification number: C12Q1/6869 , C07D209/14 , C07D401/14 , C07D405/14 , C07H19/10 , C07K5/0815 , C07K5/1019 , C09B23/0025 , C09B23/0033 , C09B23/0066 , C09B23/06 , C09B23/083 , C09B23/105 , C09B23/164 , C12Q1/6844 , C12Q1/6876 , G01N33/52 , G01N33/582 , G01N2333/9126
Abstract: The invention provides a novel class of scaffold-based labeled polymerase enzyme substrates. The polymerase enzyme substrates have a multivalent core or scaffold to which is attached fluorescent dye moieties and nucleoside phosphate moities. The polymerase enzyme substrates have multiple fluorescent dye moities and/or multiple nucleoside phosphate moieties. Preferred multivalent cores comprise trifunctional six membered aromatic moities. The invention also provides for sequencing methods and kits with scaffold-based labeled polymerase enzyme substrates.
Abstract translation: 本发明提供了一类新型的基于支架的标记聚合酶底物。 聚合酶底物具有连接有荧光染料部分和核苷磷酸酯多糖的多价核心或支架。 聚合酶底物具有多个荧光染料体系和/或多个核苷磷酸酯部分。 优选的多价核心包括三官能六元芳族部分。 本发明还提供了使用基于支架的标记聚合酶底物的测序方法和试剂盒。
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公开(公告)号:US08834847B2
公开(公告)日:2014-09-16
申请号:US13208151
申请日:2011-08-11
Applicant: Stephen Yue , Robert Weber , Xiangxu Kong , Andrei Fedorov , John Lyle
Inventor: Stephen Yue , Robert Weber , Xiangxu Kong , Andrei Fedorov , John Lyle
IPC: A61B5/00 , C12Q1/68 , C12Q1/25 , G01N33/52 , G01N33/543
CPC classification number: C12Q1/6869 , C12N13/00 , C12Q1/25 , G01N33/52 , G01N33/54373 , G01N2333/91245 , G01N2333/916
Abstract: Compositions, devices, systems and methods for reducing and/or preventing photo-induced damage of one or more reactants in an illuminated analytical reaction by addition of one or more photoprotective compounds to the reaction mixture and allowing the reaction to proceed for a period that is less than a photo-induced damage threshold period.
Abstract translation: 用于通过向反应混合物中加入一种或多种光保护性化合物并允许反应进行一段时间,以减少和/或防止照明分析反应中的一种或多种反应物的光诱导损伤的组合物,装置,系统和方法 小于光致伤害阈值期。
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公开(公告)号:US08415477B2
公开(公告)日:2013-04-09
申请号:US13149392
申请日:2011-05-31
Applicant: Gayle Buller , Jixiang Liu , Stephen Yue , Jolene Bradford
Inventor: Gayle Buller , Jixiang Liu , Stephen Yue , Jolene Bradford
IPC: C07D413/04 , C07D417/04
CPC classification number: C09B23/0075 , C07D409/04 , C07D413/04 , C07D413/14 , C07D417/04 , G01N21/6486 , G01N33/52 , G01N33/582 , G01N2021/6441
Abstract: The present invention provides dye compounds optimally excited at about 400 nm and have a Stokes shift of at least about 80 nm. These dyes find use in detection of analyte in a sample and the preparation of dye-conjugates.
Abstract translation: 本发明提供了在约400nm处最佳激发并具有至少约80nm的斯托克斯位移的染料化合物。 这些染料可用于检测样品中的分析物和染料缀合物的制备。
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公开(公告)号:US07871773B2
公开(公告)日:2011-01-18
申请号:US11756765
申请日:2007-06-01
Applicant: Richard Haugland , Stephen Yue
Inventor: Richard Haugland , Stephen Yue
IPC: C12Q1/68 , C12Q1/00 , C12Q1/02 , G01N33/53 , C07D215/00 , C07D417/00 , C12P19/34
CPC classification number: C09B23/02 , C12Q1/6816 , C12Q1/6876 , G01N1/30 , G01N27/44726 , G01N33/52 , G01N33/5308 , G01N33/531 , G01N33/582 , G01N33/68 , G01N2001/302 , Y10S436/80 , Y10T436/143333 , C12Q2563/173 , C12Q2563/107
Abstract: Unsymmetrical cyanine dyes that incorporate an aza-benzazolium ring moiety are described, including cyanine dyes substituted by a cationic side chain, monomeric and dimeric cyanine dyes, chemically reactive cyanine dyes, and conjugates of cyanine dyes. The subject dyes are virtually non-fluorescent when diluted in aqueous solution, but exhibit bright fluorescence when associated with nucleic acid polymers such as DNA or RNA, or when associated with detergent-complexed proteins. A variety of applications are described for detection and quantitation of nucleic acids and detergent-complexed proteins in a variety of samples, including solutions, electrophoretic gels, cells, and microorganisms.
Abstract translation: 描述了包含氮杂 - 苯并唑鎓环部分的不对称花青染料,包括由阳离子侧链取代的花青染料,单体和二聚花青染料,化学反应性花青染料和花青染料的缀合物。 当在水溶液中稀释时,受试染料实际上是非荧光的,但当与核酸聚合物如DNA或RNA相关联时或当与洗涤剂复合的蛋白质相关时,它们显示出明亮的荧光。 描述了用于检测和定量多种样品中的核酸和洗涤剂复合蛋白质的各种应用,包括溶液,电泳凝胶,细胞和微生物。
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