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51.发明授权Mutant E. coli appa phytase enzymes and natural variants thereof, nucleic acids encoding such phytase enzymes, vectors and host cells incorporating same and methods of making and using same 有权突变大肠杆菌植酸酶及其天然变体，编码这种植酸酶的核酸，与其结合的载体和宿主细胞以及制备和使用它们的方法公开(公告)号：US07968342B2
公开(公告)日：2011-06-28
申请号：US10524234
申请日：2003-08-11
申请人： Beat O Blattmann , Aldis Darzins , John M. Davis , Lance P. Encell , Thomas B. Morrison , Gregory T. Mrachko , Volker Schellenberger
发明人： Beat O Blattmann , Aldis Darzins , John M. Davis , Lance P. Encell , Thomas B. Morrison , Gregory T. Mrachko , Volker Schellenberger
CPC分类号： C12N15/8243 , C12N9/16 , C12N15/8245
摘要： Cells producing mutant phytases having modified activity are provided, as well as the phytases so produced. Also provided are methods of making and producing such phytases and the use of the expressed phytase protein in feed as a supplement.
摘要翻译： 提供具有改变活性的产生突变体植酸酶的细胞，以及如此制备的植酸酶。 还提供了制备和生产这种植酸酶的方法以及在饲料中使用表达的植酸酶蛋白作为补充物。
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公开(公告)号：US07125695B2
公开(公告)日：2006-10-24
申请号：US10719571
申请日：2003-11-20
CPC分类号： C12N9/0006 , C07K14/245 , C12N15/102 , C12P7/18 , C12R1/01 , C12R1/19
摘要： The present invention provides methods for directing the evolution of microorganisms comprising the use of mutator genes and growth under conditions of selective pressure. The method discloses mutator genes which can be used in the methods of the present invention and provides ATCC deposits which exemplify the evolved microorganisms produced by the methods.
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公开(公告)号：US07122334B2
公开(公告)日：2006-10-17
申请号：US10899751
申请日：2004-07-26
申请人： Volker Schellenberger , Donald P. Naki , Katherine D. Collier , James T. Kellis, Jr. , Joanne Nadherny
发明人： Volker Schellenberger , Donald P. Naki , Katherine D. Collier , James T. Kellis, Jr. , Joanne Nadherny
IPC分类号： C12Q1/34
CPC分类号： C12Q1/00
摘要： A microtiter method for assaying the wash performance of new enzymes and/or new detergent formulations is described.
摘要翻译： 描述了用于测定新酶和/或新洗涤剂制剂的洗涤性能的微量滴定法。
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公开(公告)号：US20060183171A1
公开(公告)日：2006-08-17
申请号：US11378735
申请日：2006-03-17
申请人： Volker Schellenberger , Amy Liu
发明人： Volker Schellenberger , Amy Liu
IPC分类号： G01N33/53
CPC分类号： B01J19/0046 , B01J2219/00286 , B01J2219/00317 , B01J2219/00319 , B01J2219/0052 , B01J2219/00524 , B01J2219/00533 , B01J2219/00596 , B01J2219/00644 , B01J2219/00659 , B01J2219/00662 , B01J2219/00707 , B01L3/5025 , B01L3/5085 , B01L3/50857 , B01L2300/0654 , B01L2300/0809 , B01L2300/161 , C40B60/14 , G01N35/1065 , G01N2035/00237
摘要： A high throughput screening method. At least one liquid sample is provided that includes an analyte that directly or indirectly produces a detectable characteristic in one or more first assays. A plurality of through holes is filled with portions of the liquid sample in a testing assembly that has a pair of opposing surfaces, each through hole extending from one of the opposing surfaces to the other of the opposing surfaces, with the through holes arranged in group. Surface tension holds the respective portions in the respective plurality of through holes. A detection is made of which of the through holes contains a liquid sample portion that includes said analyte. The detectable characteristic may be produced in various assays such as an absorbance transcription assay, a fluorescent transcription assay, a fluorescent secreted enzyme assay, or a microorganism screening assay.
摘要翻译： 高通量筛选方法。 提供至少一种液体样品，其包括在一个或多个第一测定中直接或间接产生可检测特征的分析物。 多个通孔在具有一对相对表面的测试组件中填充有液体样品的部分，每个通孔从相对表面中的一个延伸到另一个相对表面，通孔布置成 。 表面张力保持相应的多个通孔中的各个部分。 检测哪个通孔包含包含所述分析物的液体样品部分。 可以在各种测定中产生可检测特征，例如吸光度转录测定，荧光转录测定，荧光分泌酶测定或微生物筛选测定。
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55.发明申请Mutant E. coli appa phytase enzymes and natural variants thereof, nucleic acids encoding such phytase enzymes, vectors and host cells incorporating same and methods of making and using same 有权突变大肠杆菌植酸酶及其天然变体，编码这种植酸酶的核酸，与其结合的载体和宿主细胞以及制备和使用它们的方法公开(公告)号：US20060141562A1
公开(公告)日：2006-06-29
申请号：US10524234
申请日：2003-08-11
申请人： Beat Blattmann , Aldis Darzins , John Davis , Lance Encell , Thomas Morrison , Gregory Mrachko , Volker Schellenberger
发明人： Beat Blattmann , Aldis Darzins , John Davis , Lance Encell , Thomas Morrison , Gregory Mrachko , Volker Schellenberger
CPC分类号： C12N15/8243 , C12N9/16 , C12N15/8245
摘要： Cells producing mutant phytases having modified activity are provided, as well as the phytases so produced. Also provided are methods of making and producing such phytases and the use of the expressed phytase protein in feed as a supplement.
摘要翻译： 提供具有改变活性的产生突变体植酸酶的细胞，以及如此制备的植酸酶。 还提供了制备和生产这种植酸酶的方法以及在饲料中使用表达的植酸酶蛋白作为补充物。
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公开(公告)号：US20050164308A1
公开(公告)日：2005-07-28
申请号：US11011422
申请日：2004-12-14
CPC分类号： C12N9/86
摘要： The present invention provides beta-lactamase CD4+ T-cell epitopes, as well as novel variants that exhibit reduced immunogenic responses, as compared to the parental beta-lactamase. The present invention further provides DNA molecules that encode novel beta-lactamase variants, host cells comprising DNA encoding novel beta-lactamase variants, as well as methods for making beta-lactamases less immunogenic. In addition, the present invention provides various compositions that comprise these beta-lactamase variants that are less immunogenic than wild-type beta-lactamases.
摘要翻译： 与亲本β-内酰胺酶相比，本发明提供了β-内酰胺酶CD4 + T细胞表位，以及表现出降低的免疫原性应答的新型变体。 本发明进一步提供了编码新型β-内酰胺酶变体的DNA分子，包含编码新型β-内酰胺酶变体的DNA的宿主细胞，以及制备较少免疫原性的β-内酰胺酶的方法。 此外，本发明提供了包含与野生型β-内酰胺酶相比免疫原性较低的这些β-内酰胺酶变体的各种组合物。
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公开(公告)号：US06838425B2
公开(公告)日：2005-01-04
申请号：US10047689
申请日：2002-01-15
申请人： Chanchal Kumar Ghosh , André Cesar Baeck , Ryohei Ohtani , Alfred Busch , Michael Stanford Showell , Ayrookaran J. Poulose , Volker Schellenberger , James T. Kellis, Jr. , Christian Paech , Joanne Nadherny , Donald P. Naki , Katherine D. Collier , Robert M. Caldwell
发明人： Chanchal Kumar Ghosh , André Cesar Baeck , Ryohei Ohtani , Alfred Busch , Michael Stanford Showell , Ayrookaran J. Poulose , Volker Schellenberger , James T. Kellis, Jr. , Christian Paech , Joanne Nadherny , Donald P. Naki , Katherine D. Collier , Robert M. Caldwell
CPC分类号： C11D3/386
摘要： The present invention relates to cleaning compositions comprising a protease variant. One cleaning composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 of Bacillus amyloliquefaciens subtilisin in combination with a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 1, 3, 4, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 and 275 of Bacillus amyloliquefaciens subtilisin; wherein when said protease variant includes a substitution of amino acid residues at positions corresponding to positions 103 and 76, there is also a subtitution of an amino acid residue at one or more amino acid residue positions other than amino acid residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265 or 274 of Bacillus amyloliquefaciens subtilisin; and one or more cleaning adjunct materials. Another cleaning composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin; and one or more cleaning adjunct materials. Methods for using the cleaning compositions are also provided.
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公开(公告)号：US06831053B1
公开(公告)日：2004-12-14
申请号：US09529904
申请日：2000-04-20
申请人： Chanchal Kumar Ghosh , André Cesar Baeck , Ryohei Ohtani , Alfred Busch , Michael Stanford Showell , Ayrookaran J. Poulose , Volker Schellenberger , James T. Kellis, Jr. , Christian Paech , Joanne Nadherny , Donald P. Naki , Katherine D. Collier , Robert M. Caldwell
发明人： Chanchal Kumar Ghosh , André Cesar Baeck , Ryohei Ohtani , Alfred Busch , Michael Stanford Showell , Ayrookaran J. Poulose , Volker Schellenberger , James T. Kellis, Jr. , Christian Paech , Joanne Nadherny , Donald P. Naki , Katherine D. Collier , Robert M. Caldwell
IPC分类号： C11D300
CPC分类号： A61Q19/10 , A61K8/38 , A61K8/66 , C11D3/386 , C11D3/3907 , C11D3/3945
摘要： The present invention relates to bleaching compositions comprising a protease variant. One bleaching composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at an amino acid residue position corresponding to position 103 of Bacillus amyloliquefaciens subtilisin in combination with a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 1, 3, 4, 8, 9, 10, 12, 13, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274, and 275 of Bacillus amyloliquefaciens subtilisin; wherein when said protease variant includes a substitution of amino acid residues at positions corresponding to positions 103 and 76, there is also a substitution of an amino acid residue at one or more amino acid residue positions other than amino acid residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265, or 274 of Bacillus amyloliquefaciens subtilisin, a bleaching agent; and one or more cleaning adjunct materials. Another bleaching composition comprises a protease variant including a substitution of an amino acid residue with another naturally occurring amino acid residue at one or more amino acid residue positions corresponding to positions 62, 212, 230, 232, 252 and 257 of Bacillus amyloliquefaciens subtilisin; a bleaching agent; and one or more cleaning adjunct materials. Methods for using the bleaching compositions are also provided.
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公开(公告)号：US06815193B2
公开(公告)日：2004-11-09
申请号：US10033325
申请日：2001-11-02
申请人： Ayrookaran J. Poulose , Volker Schellenberger , James T. Kellis, Jr. , Christian Paech , Joanne Nadherny , Donald P. Naki , Katherine D. Collier , Robert M. Caldwell , André C. Baeck
发明人： Ayrookaran J. Poulose , Volker Schellenberger , James T. Kellis, Jr. , Christian Paech , Joanne Nadherny , Donald P. Naki , Katherine D. Collier , Robert M. Caldwell , André C. Baeck
IPC分类号： C12N956
CPC分类号： C12N9/54 , A23G4/123 , A23K20/189 , A61K8/66 , A61K2800/86 , A61Q11/00 , A61Q19/00 , C11D3/386 , C11D3/38609 , C11D3/38618 , C11D3/3907 , C11D3/3945 , C12Y304/00 , C12Y304/21062
摘要： Novel protease variants derived from the DNA sequences of naturally-occurring or recombinant non-human proteases are disclosed. The variant proteases, in general, are obtained by in vitro modification of a precursor DNA sequence encoding the naturally-occurring or recombinant protease to generate the substitution of a plurality of amino acid residues in the amino acid sequence of a precursor protease. Such variant proteases have properties which are different from those of the precursor protease, such as altered wash performance. The substituted amino acid residue corresponds to position 103 in combination with one or more of the following substitutions at residue positions corresponding to positions 1, 3, 4, 8, 10, 12, 13, 15, 16, 17, 18, 19, 20, 21, 22, 24, 27, 33, 37, 38, 42, 43, 48, 55, 57, 58, 61, 62, 68, 72, 75, 76, 77, 78, 79, 86, 87, 89, 97, 98, 99, 101, 102, 104, 106, 107, 109, 111, 114, 116, 117, 119, 121, 123, 126, 128, 130, 131, 133, 134, 137, 140, 141, 142, 146, 147, 158, 159, 160, 166, 167, 170, 173, 174, 177, 181, 182, 183, 184, 185, 188, 192, 194, 198, 203, 204, 205, 206, 209, 210, 211, 212, 213, 214, 215, 216, 217, 218, 222, 224, 227, 228, 230, 232, 236, 237, 238, 240, 242, 243, 244, 245, 246, 247, 248, 249, 251, 252, 253, 254, 255, 256, 257, 258, 259, 260, 261, 262, 263, 265, 268, 269, 270, 271, 272, 274 and 275 of Bacillus amyloliquefaciens subtilisin, wherein when a substitution at a position corresponding to residue position 103 is combined with a substitution at a position corresponding to residue position 76, there is also a substitution at one or more residue positions other than residue positions corresponding to positions 27, 99, 101, 104, 107, 109, 123, 128, 166, 204, 206, 210, 216, 217, 218, 222, 260, 265, or 274 of Bacillus amyloliquefaciens subtilisin.
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公开(公告)号：US06706503B2
公开(公告)日：2004-03-16
申请号：US10037677
申请日：2001-10-23
IPC分类号： C12P104
CPC分类号： C12N9/0006 , C07K14/245 , C12N15/102 , C12P7/18 , C12R1/01 , C12R1/19
摘要： The present invention provides methods for directing the evolution of microorganisms comprising the use of mutator genes and growth under conditions of selective pressure. The method discloses mutator genes which can be used in the methods of the present invention and provides ATCC deposits which exemplify the evolved microorganisms produced by the methods.
摘要翻译： 本发明提供了用于引导微生物进化的方法，所述微生物包括使用突变基因并在选择压力条件下生长。 该方法公开了可用于本发明方法的突变基因，并提供了例证通过该方法产生的进化微生物的ATCC沉积物。
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