公开(公告)号：US20070263290A1

公开(公告)日：2007-11-15

申请号：US11744797

申请日：2007-05-04

申请人： Patrick Treado ,  Matthew Nelson ,  Scott Keitzer ,  Ryan Smith

发明人： Patrick Treado ,  Matthew Nelson ,  Scott Keitzer ,  Ryan Smith

IPC分类号： G02B5/04 ,  G02B3/00 ,  G02B5/22

CPC分类号： G02B6/06 ,  A61B1/00165 ,  A61B1/043 ,  A61B1/063 ,  A61B1/0638 ,  A61B1/0646 ,  A61B1/07 ,  A61B5/0075 ,  A61B5/0084 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/0218 ,  G01J3/0227 ,  G01J3/024 ,  G01J3/0256 ,  G01J3/0262 ,  G01J3/0286 ,  G01J3/44 ,  G01J2003/1213

摘要： A fiberscope device is disclosed which is suitable for video imaging, laser Raman spectroscopy and laser Raman spectroscopic (i.e. chemical) imaging. The fiberscope design minimizes fiber background interference arising from the laser delivery fiber optic and the coherent fiber optic light gathering bundle while maintaining high light throughput efficiency through the use of integrated spectral filters. In the fiberscope design, the laser delivery fiber optic is offset from the coherent fiber optic light gathering bundle. The laser delivery field is captured entirely by the light gathering field of view of the coherent fiber bundle. The fiberscope incorporates spectral filter optical elements that provide environmental insensitivity, particularly to temperature and moisture. The fiberscope is suited to the analysis of a wide range of condensed phase materials (solids and liquids), including the analysis of biological materials such as breast tissue lesions and arterial plaques, in such a manner to delineate abnormal from normal tissues.

摘要翻译： 公开了适用于视频成像，激光拉曼光谱和激光拉曼光谱（即化学）成像的纤维内窥镜装置。 纤维镜设计可以最大限度地减少激光传输光纤和相干光纤采集光束产生的光纤背景干扰，同时通过使用集成光谱滤波器保持高光通量效率。 在纤维镜设计中，激光传输光纤与相干光纤聚光束偏移。 激光输送场完全由相干光纤束的聚光场捕获。 纤维镜包含光谱滤光器光学元件，其提供环境不敏感性，特别是对温度和湿度。 纤维内窥镜适用于分析各种凝聚相材料（固体和液体），包括分析生物材料如乳房组织损伤和动脉斑块，以便从正常组织中描绘异常。

公开(公告)号：US20070182959A1

公开(公告)日：2007-08-09

申请号：US10556038

申请日：2005-06-30

申请人： John Maier ,  Patrick Treado ,  David Tuschel ,  Thomas Voigt

发明人： John Maier ,  Patrick Treado ,  David Tuschel ,  Thomas Voigt

IPC分类号： G01J3/44 ,  G01N21/65

CPC分类号： G01N21/6456 ,  G01J3/2803 ,  G01J3/32 ,  G01J3/36 ,  G01J3/44 ,  G01N21/359 ,  G01N21/6486 ,  G01N21/65 ,  G01N21/658 ,  G01N2021/656

摘要： The invention relates to methods of dynamic chemical imaging, including methods of cellular imaging. The method comprises illuminating at least a portion of a cell with substantially monochromatic light and assessing Raman-shifted light scattered from the illuminated portion at a plurality of discrete times. The Raman-shifted light can be assessed at a plurality of Raman shift (RS) values at each of the discrete times, and the RS values can be selected to be characteristic of a pre-selected component at each of the discrete times. Multivariate analysis of Raman spectral features of the images thus obtained can yield the location and chemical identity of components in the field of view. This information can be combined or overlaid with other spectral data (e.g., a visible microscopic image) obtained from the field of view.

摘要翻译： 本发明涉及动态化学成像方法，包括细胞成像方法。 该方法包括以基本上单色的光照亮电池的至少一部分，并且在多个离散时间评估从照射部分散射的拉曼偏移光。 可以在每个离散时间处以多个拉曼位移（RS）值评估拉曼移位的光，并且可以将每个离散时间的RS值选择为预选择的分量的特征。 如此获得的图像的拉曼光谱特征的多变量分析可以产生视场中部件的位置和化学特性。 该信息可以与从视野获得的其他光谱数据（例如，可见显微镜图像）组合或覆盖。

公开(公告)号：US20070145258A1

公开(公告)日：2007-06-28

申请号：US11640197

申请日：2006-12-18

申请人： Matthew Nelson ,  Patrick Treado

发明人： Matthew Nelson ,  Patrick Treado

IPC分类号： G01D18/00

CPC分类号： G01J3/28 ,  A61B5/0059 ,  A61B2560/0223 ,  G01J2003/2866

摘要： The disclosure generally relates to a method and apparatus for automated spectral calibration of a spectroscopy device. In one embodiment, the disclosure relates to a method for simultaneous calibration and spectral imaging of a sample by: simultaneously illuminating the sample and a calibrant with a plurality of illuminating photons; receiving, at the spectrometer, a first plurality of photons collected from the sample and a second plurality of photons collected from the calibrant; forming a calibrant spectrum from the first plurality of collected photons and a sample spectrum from the second plurality of collected photons; comparing the calibrant spectrum with a reference spectrum of the calibrant to determine a wavelength-shift in the calibrant spectrum; applying the wavelength-shift to the sample spectrum to obtain a calibrated sample spectrum.

摘要翻译： 本发明一般涉及一种用于光谱设备的自动光谱校准的方法和装置。 在一个实施方案中，本公开涉及通过以下步骤同时校准和光谱成像的方法：同时用多个照明光子照射样品和校准物; 在光谱仪处接收从样品收集的第一组多个光子和从校准物收集的第二组多个光子; 从所述第一多个收集的光子形成校准谱，以及来自所述第二多个收集的光子的样品光谱; 将校准谱与校准物的参考光谱进行比较，以确定校准谱中的波长漂移; 将波长偏移应用于样本光谱以获得校准的样本光谱。

公开(公告)号：US20060281068A1

公开(公告)日：2006-12-14

申请号：US11269596

申请日：2005-11-09

申请人： John Maier ,  Shona Stewart ,  Patrick Treado

发明人： John Maier ,  Shona Stewart ,  Patrick Treado

IPC分类号： C12Q1/00 ,  C12Q1/70 ,  C12Q1/68 ,  G06F19/00 ,  G01J3/44

CPC分类号： G01N21/65 ,  G01N2021/656

摘要： Raman molecular imaging (RMI) is used to detect mammalian cells of a particular phenotype. For example the disclosure includes the use of RMI to differentiate between normal and diseased cells or tissues, e.g., cancer cells as well as in determining the grade of said cancer cells. In a preferred embodiment benign and malignant lesions of bladder and other tissues can be distinguished, including epithelial tissues such as lung, prostate, kidney, breast, and colon, and non-epithelial tissues, such as bone marrow and brain. Raman scattering data relevant to the disease state of cells or tissue can be combined with visual image data to produce hybrid images which depict both a magnified view of the cellular structures and information relating to the disease state of the individual cells in the field of view. Also, RMI techniques may be combined with visual image data and validated with other detection methods to produce confirm the matter obtained by RMI.

摘要翻译： 拉曼分子成像（RMI）用于检测特定表型的哺乳动物细胞。 例如，本公开包括使用RMI来区分正常和患病细胞或组织，例如癌细胞，以及确定所述癌细胞的等级。 在一个优选实施方案中，可以区分膀胱和其他组织的良性和恶性病变，包括上皮组织如肺，前列腺，肾，乳腺和结肠以及非上皮组织，例如骨髓和脑。 可以将与细胞或组织的疾病状态相关的拉曼散射数据与视觉图像数据组合，以产生描绘细胞结构的放大视图和与视野中的各个细胞的疾病状态有关的信息的混合图像。 此外，RMI技术可以与可视图像数据组合，并用其他检测方法验证，以确定RMI获得的事项。

公开(公告)号：US20060192956A1

公开(公告)日：2006-08-31

申请号：US11257219

申请日：2005-10-24

申请人： Patrick Treado ,  Matthew Nelson ,  Scott Keitzer

发明人： Patrick Treado ,  Matthew Nelson ,  Scott Keitzer

IPC分类号： G01J3/28

CPC分类号： G01N21/359 ,  G01J3/2823 ,  G02B21/0016 ,  G02B21/06

摘要： A chemical imaging system is provided which uses a near infrared radiation microscope. The system includes an illumination source which illuminates an area of a sample using light in the near infrared radiation wavelength and light in the visible wavelength. A multitude of spatially resolved spectra of transmitted, reflected, emitted or scattered near infrared wavelength radiation light from the illuminated area of the sample is collected and a collimated beam is produced therefrom. A near infrared imaging spectrometer is provided for selecting a near infrared radiation image of the collimated beam. The filtered images are collected by a detector for further processing. The visible wavelength light from the illuminated area of the sample is simultaneously detected providing for the simultaneous visible and near infrared chemical imaging analysis of the sample. Two efficient means for performing three dimensional near infrared chemical imaging microscopy are provided.

摘要翻译： 提供使用近红外辐射显微镜的化学成像系统。 该系统包括使用近红外辐射波长的光和可见波长的光来照射样品的区域的照明源。 收集来自样品的照射区域的透射，反射，发射或散射的近红外波长辐射光的多个空间解析光谱，并从其中产生准直光束。 提供近红外成像光谱仪用于选择准直光束的近红外辐射图像。 滤波后的图像由检测器收集以进一步处理。 同时检测来自样品的照射区域的可见波长的光，提供样品的同时可见和近红外化学成像分析。 提供了三维近红外化学成像显微镜的两种有效手段。

公开(公告)号：US20060151702A1

公开(公告)日：2006-07-13

申请号：US11366129

申请日：2006-03-02

申请人： Patrick Treado ,  Matthew Nelson ,  Scott Keitzer

发明人： Patrick Treado ,  Matthew Nelson ,  Scott Keitzer

IPC分类号： G01N21/35

CPC分类号： G01N21/359 ,  G01J3/2823 ,  G02B21/0016 ,  G02B21/06

摘要： A chemical imaging system is provided which uses a near infrared radiation microscope. The system includes an illumination source which illuminates an area of a sample using light in the near infrared radiation wavelength and light in the visible wavelength. A multitude of spatially resolved spectra of transmitted, reflected, emitted or scattered near infrared wavelength radiation light from the illuminated area of the sample is collected and a collimated beam is produced therefrom. A near infrared imaging spectrometer is provided for selecting a near infrared radiation image of the collimated beam. The filtered images are collected by a detector for further processing. The visible wavelength light from the illuminated area of the sample is simultaneously detected providing for the simultaneous visible and near infrared chemical imaging analysis of the sample. Two efficient means for performing three dimensional near infrared chemical imaging microscopy are provided.

公开(公告)号：US20060146315A1

公开(公告)日：2006-07-06

申请号：US11364180

申请日：2006-03-01

申请人： Patrick Treado

发明人： Patrick Treado

IPC分类号： G01J3/44 ,  G01N21/64 ,  G01N21/65

CPC分类号： G01N21/65 ,  G01J3/02 ,  G01J3/0224 ,  G01J3/0229 ,  G01J3/0256 ,  G01J3/0264 ,  G01J3/0272 ,  G01J3/0283 ,  G01J3/10 ,  G01J3/44 ,  G01J3/4406 ,  G01N21/6456 ,  G01N2021/6417 ,  G01N2021/6423 ,  G01N2021/6471 ,  G01N2201/0221 ,  G01N2201/0627 ,  G02F1/133371 ,  G02F1/1347

摘要： The disclosure generally relates to a method and apparatus for compact dispersive imaging spectrometer. More specifically, one embodiment of the disclosure relates to a portable system for obtaining a spatially accurate wavelength-resolved image of a sample having a first and a second spatial dimension. The portable system can include a photon emission source for sequentially illuminating a plurality of portions of said sample with a plurality of photons to produce photons scattered by the sample. The photon emission source can illuminate the sample along the first spatial dimension for each of plural predetermined positions of the second spatial dimension. The system may also include an optical lens for collecting the scattered photons to produce therefrom filtered photons, a dispersive spectrometer for determining a wavelength of ones of the filtered photons, a photon detector for receiving the filtered photons and obtaining therefrom plural spectra of said sample, and a processor for producing a two dimensional image of said sample from the plural spectra.

摘要翻译： 本发明一般涉及紧凑型分散成像光谱仪的方法和装置。 更具体地，本公开的一个实施例涉及用于获得具有第一和第二空间维度的样本的空间上准确的波长分辨图像的便携式系统。 便携式系统可以包括光子发射源，用于用多个光子顺序照射所述样品的多个部分，以产生由样品散射的光子。 光子发射源可以沿着第一空间维度对第二空间维度的多个预定位置中的每一个照射样本。 该系统还可以包括用于收集散射光子以产生滤光光子的光学透镜，用于确定滤光光子中波长的波长的色散光谱仪，用于接收经滤光的光子并由此获得所述样品的多个光谱的光子检测器， 以及用于从所述多个光谱产生所述样本的二维图像的处理器。

公开(公告)号：US20060050278A1

公开(公告)日：2006-03-09

申请号：US11229539

申请日：2005-09-20

申请人： Patrick Treado ,  Charles Gardner ,  Matthew Nelson

发明人： Patrick Treado ,  Charles Gardner ,  Matthew Nelson

IPC分类号： G01N21/25

CPC分类号： G01N21/255

摘要： The disclosure relates to method and apparatus for obtaining a multimodal hyperspectral image of a sample for widefield spectral analysis. An apparatus according to one embodiment may include a plurality of optical lenses configured to interchangeably receive photons and focus the photons; a plurality of tunable filters positioned in a filter housing, each tunable filter selectively receiving the focused dispersed photons from one of the plurality of optical lenses, each tunable filter providing wavelength-selective filtered photons; a first optical camera and a second optical cameras for selectively receiving the wavelength-selective filtered photons from each of the plurality of tunable filters and combining said filtered photons to form a hyperspectral spatially accurate, wavelength-resolved image of the sample.

摘要翻译： 本公开涉及用于获得用于宽带光谱分析的样品的多峰高光谱图像的方法和装置。 根据一个实施例的装置可以包括多个配置成互换地接收光子并聚焦光子的光学透镜; 多个可调滤光器，其位于过滤器壳体中，每个可调谐滤光器选择性地从多个光学透镜中的一个透镜接收聚焦的分散的光子，每个可调谐滤光器提供波长选择性滤光的光子; 第一光学摄像机和第二光学摄像机，用于从多个可调谐滤光器中的每一个选择性地接收波长选择性滤波的光子，并组合所述滤波的光子以形成样品的高光谱空间准确的波长分辨图像。

公开(公告)号：US20060049354A1

公开(公告)日：2006-03-09

申请号：US11257139

申请日：2005-10-24

申请人： Patrick Treado ,  Matthew Nelson ,  Scott Keitzer

发明人： Patrick Treado ,  Matthew Nelson ,  Scott Keitzer

IPC分类号： G01J5/02

CPC分类号： G01N21/359 ,  G01J3/2823 ,  G02B21/0016 ,  G02B21/06

摘要： A chemical imaging system is provided which uses a near infrared radiation microscope. The system includes an illumination source which illuminates an area of a sample using light in the near infrared radiation wavelength and light in the visible wavelength. A multitude of spatially resolved spectra of transmitted, reflected, emitted or scattered near infrared wavelength radiation light from the illuminated area of the sample is collected and a collimated beam is produced therefrom. A near infrared imaging spectrometer is provided for selecting a near infrared radiation image of the collimated beam. The filtered images are collected by a detector for further processing. The visible wavelength light from the illuminated area of the sample is simultaneously detected providing for the simultaneous visible and near infrared chemical imaging analysis of the sample. Two efficient means for performing three dimensional near infrared chemical imaging microscopy are provided.

公开(公告)号：US20060036181A1

公开(公告)日：2006-02-16

申请号：US11204196

申请日：2005-08-09

申请人： Patrick Treado ,  Matthew Nelson

发明人： Patrick Treado ,  Matthew Nelson

IPC分类号： A61B6/00 ,  A61B18/18 ,  G01J3/44 ,  G01J3/40 ,  A61B5/00

CPC分类号： A61B5/0091 ,  A61B5/4312 ,  G01N21/65

摘要： Apparatus and methods for spatially resolved Raman chemical imaging of breast tissue is disclosed. A region of breast tissue is illuminated with monochromatic light. A spatially organized area of endogenous molecules in the tissue is then detected in the region by detecting a Raman shifted light signal. The Raman shifted light signal is spatially resolved in at least one direction and is thus useful for examining breast tissue, especially to detect malignant tissue.

摘要翻译： 公开了用于乳腺组织的空间分辨拉曼化学成像的装置和方法。 乳腺组织区域用单色光照射。 然后通过检测拉曼移动的光信号在该区域中检测组织中的内分子的空间有组织的区域。 拉曼移动的光信号在至少一个方向上被空间分辨，因此可用于检查乳腺组织，特别是用于检测恶性组织。